• Title/Summary/Keyword: 3D (3Dimensional)

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Identification of Diapause and Non-diapause Associated Proteins in the Eggs of Multivoltine Silkworm Bombyx mori by MALDI MS Analysis

  • Saravanakumar, Ragunathan;Ponnuvel, Kangayam M.;Velu, Dhanikachalam;Koundinya, Panguluri R.;Sinha, Randir K.;Qadri, Syed M. Hussaini
    • International Journal of Industrial Entomology and Biomaterials
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    • v.16 no.2
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    • pp.37-48
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    • 2008
  • The diapause and non diapause associated proteins of multivoltine silkworm eggs were analysed by two dimensional (2D) gel electrophoresis. The study was made at 0 hr, 24 hrs and 48 hrs after oviposition. A total of four protein spots in diapause eggs at 24 hrs of oviposition and two protein spots in non diapause eggs at 0 hrs of oviposition were observed. All the six protein spots were considered to have association with diapause and non diapause characters. The molecular weight (MW) and isoelectric point (PI) of these 6 protein spots were calculated. The protein spots 1 and 2 observed in 0 hr of non diapause eggs were found to have the MW of 67 and 75 KDa and PI of 8.6 and 8.4 respectively. Similarly the four protein spots observed in diapause egg at 24 hrs of oviposition exhibited MW viz., 15, 17,20 and 25 KDa and PI of 5.3, 5.8, 6.5 and 6.0 respectively. All these 6 identified protein spots were subjected to in-gel digestion and resulted tryptic peptides were analyzed by Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI TOF-MS). Databases searched based on experimentally determined molecular weights of peptides for the determination of the identities of proteins. The identified proteins indicated homology of 34% to 95%. The results indicate that the proteins may playa role in development of diapause and non diapause eggs.

Evaluation of Accuracy and Inaccuracy of Depth Sensor based Kinect System for Motion Analysis in Specific Rotational Movement for Balance Rehabilitation Training (균형 재활 훈련을 위한 특정 회전 움직임에서 피검자 동작 분석을 위한 깊이 센서 기반 키넥트 시스템의 정확성 및 부정확성 평가)

  • Kim, ChoongYeon;Jung, HoHyun;Jeon, Seong-Cheol;Jang, Kyung Bae;Chun, Keyoung Jin
    • Journal of Biomedical Engineering Research
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    • v.36 no.5
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    • pp.228-234
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    • 2015
  • The balance ability significantly decreased in the elderly because of deterioration of the neural musculature regulatory mechanisms. Several studies have investigated methods of improving balance ability using real-time systems, but it is limited by the expensive test equipment and specialized resources. Recently, Kinect system based on depth data has been applied to address these limitations. Little information about accuracy/inaccuracy of Kinect system is, however, available, particular in motion analysis for evaluation of effectiveness in rehabilitation training. Therefore, the aim of the current study was to evaluate accuracy/inaccuracy of Kinect system in specific rotational movement for balance rehabilitation training. Six healthy male adults with no musculoskeletal disorder were selected to participate in the experiment. Movements of the participants were induced by controlling the base plane of the balance training equipment in directions of AP (anterior-posterior), ML (medial-lateral), right and left diagonal direction. The dynamic motions of the subjects were measured using two Kinect depth sensor systems and a three-dimensional motion capture system with eight infrared cameras for comparative evaluation. The results of the error rate for hip and knee joint alteration of Kinect system comparison with infrared camera based motion capture system occurred smaller values in the ML direction (Hip joint: 10.9~57.3%, Knee joint: 26.0~74.8%). Therefore, the accuracy of Kinect system for measuring balance rehabilitation traning could improve by using adapted algorithm which is based on hip joint movement in medial-lateral direction.

SHM benchmark for high-rise structures: a reduced-order finite element model and field measurement data

  • Ni, Y.Q.;Xia, Y.;Lin, W.;Chen, W.H.;Ko, J.M.
    • Smart Structures and Systems
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    • v.10 no.4_5
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    • pp.411-426
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    • 2012
  • The Canton Tower (formerly named Guangzhou New TV Tower) of 610 m high has been instrumented with a long-term structural health monitoring (SHM) system consisting of over 700 sensors of sixteen types. Under the auspices of the Asian-Pacific Network of Centers for Research in Smart Structures Technology (ANCRiSST), an SHM benchmark problem for high-rise structures has been developed by taking the instrumented Canton Tower as a host structure. This benchmark problem aims to provide an international platform for direct comparison of various SHM-related methodologies and algorithms with the use of real-world monitoring data from a large-scale structure, and to narrow the gap that currently exists between the research and the practice of SHM. This paper first briefs the SHM system deployed on the Canton Tower, and the development of an elaborate three-dimensional (3D) full-scale finite element model (FEM) and the validation of the model using the measured modal data of the structure. In succession comes the formulation of an equivalent reduced-order FEM which is developed specifically for the benchmark study. The reduced-order FEM, which comprises 37 beam elements and a total of 185 degrees-of-freedom (DOFs), has been elaborately tuned to coincide well with the full-scale FEM in terms of both modal frequencies and mode shapes. The field measurement data (including those obtained from 20 accelerometers, one anemometer and one temperature sensor) from the Canton Tower, which are available for the benchmark study, are subsequently presented together with a description of the sensor deployment locations and the sensor specifications.

The influence of tunnelling on the behaviour of pre-existing piled foundations in weathered soil

  • Lee, Cheol-Ju;Jeon, Young-Jin;Kim, Sung-Hee;Park, Inn-Joon
    • Geomechanics and Engineering
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    • v.11 no.4
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    • pp.553-570
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    • 2016
  • A series of three-dimensional (3D) parametric finite element analyses have been performed to study the influence of the relative locations of pile tips with regards to the tunnel position on the behaviour of single piles and pile groups to adjacent tunnelling in weathered soil. When the pile tips are inside the influence zone, which considers the relative pile tip location with respect to the tunnel position, tunnelling-induced pile head settlements are larger than those computed from the Greenfield condition. However, when the pile tips are outside the influence zone, a reverse trend is obtained. When the pile tips are inside the influence zone, the tunnelling-induced tensile pile forces mobilised, but when the pile tips are outside the influence zone, compressive pile forces are induced because of tunnelling, depending on the shear stress transfer mechanism at the pile-soil interface. For piles connected to a cap, tensile and compressive forces are mobilised at the top of the centre and side piles, respectively. It has been shown that the increases in the tunnelling-induced pile head settlements have resulted in reductions of the apparent factor of safety up to approximately 43% when the pile tips are inside the influence zone, therefore severely affecting the serviceability of the piles. The pile behaviour, when considering the location of the pile tips with regards to the tunnel, has been analysed in great detail by taking the tunnelling-induced pile head settlements, axial pile forces, apparent factor of safety of the piles and shear transfer mechanism into account.

Characteristics of Magnetic Resonance(M.R.) and Comprehension of its Imaging Mechanism (자기공명(M.R.)진단법의 특징 및 그 영상기전의 이해)

  • Chang, Jae-Chun;Hwang, Mi-Soo;Kim, Sun-Yong
    • Journal of Yeungnam Medical Science
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    • v.4 no.1
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    • pp.1-15
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    • 1987
  • Magnetic Resonance (M.R.) is rapidly emerging technique that provides high quality images and potentially provides much more diagnostic information than do conventional imaging modalities. M.R.I. is conceptually quite different from currently used imaging methods. The complex nature of M.R.I. allows a great deal of flexibility in image product ion and available information, and key points are as follows. 1. M.R.I. offers a non-invasive technique with which to gene rate in vivo human images without ionizing radiation and with no known adverse biological effects. 2. Imaging mechanism of M.R.I. is quite different from conventional imaging modality and for more accurate diagnostic application, It is necessary for physician to understand imaging mechanism of M.R.I. 3. M.R. makes available basic chemical parameters that may provide to be useful for diagnostic medical imaging and more specific pathophysiologic information which are not available by alternate techniques. 4. M.R. can be produced by number of different methods. This flexibility allows the imaging technique to be applicated for particular clinical purpose. Multiplanar and three dimensional imaging may extend the imaging process beyond the single section available with current CT. 5. Future directions include efforts to; a. Further development of hard ware b. More fasternning scan time c. Respiratory and cardiac gated imaging d. Imaging of additional nuclei except hydrogen e. Further development of contrast media f. M.R. in vivo spectroscopy g. Real time M.R. imaging.

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On the Suitable Shape of Bottom for the Application of Air Cavity on Hull Bottom to the Practical Hull Form (선저부 공기공동을 이용한 실선선형의 저항성능 개선을 위한 선저형상 개량연구)

  • Seok-Cheon Go;Hyo-Chul Kim
    • Journal of the Society of Naval Architects of Korea
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    • v.36 no.3
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    • pp.1-7
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    • 1999
  • This paper describes the modification of hull bottom for the air lubrication technique to the passenger boat in service at the Chung-Ju lake, which has a large beam-draft ratio. From numerical analysis of 2-D cavity problem by potential theory, the cavity shape, length and the pressure in cavity are estimated for the simplified geometry of hull bottom, and the non-dimensional parameters affecting air cavity phenomena are investigated. Extensive resistance tests for the model ship which has variation of step height and side strip have been performed to investigate the formation of air cavity and the drag reduction effectiveness. And also, the development of attached cavity to the bottom were observed from the flat bottom made by transparent acrylic plate. From this survey on the modification of bottom shape and the air lubrication technique, the total resistance of model ship could be reduced by about 25% at the design speed compared to the proto type hull form.

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Comparison of time and cost between conventional surgical planning and virtual surgical planning in orthognathic surgery in Korea

  • Park, Si-Yeon;Hwang, Dae-Seok;Song, Jae-Min;Kim, Uk-Kyu
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.41
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    • pp.35.1-35.7
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    • 2019
  • Background: The purpose of this study was to measure the time of the conventional surgical planning (CSP) and virtual surgical planning (VSP) in orthognathic surgery and to compare them in terms of cost. Material and method: This is a retrospective study of the patients who underwent orthognathic surgery at the Pusan National University Dental Hospital from December 2017 to August 2018. All the patients were analyzed through both CSP and VSP, and all the surgical stents were fabricated through manual and three-dimensional (3D) printing. The predictor variables were the planning method (CSP vs. VSP) and the surgery type (group I: Le Fort I osteotomy + bilateral sagittal split osteotomy [LFI+BSSO] or group II: only bilateral sagittal split osteotomy [BSSO]), and the outcomes were the time and cost. The results were analyzed using the paired t test. Results: Thirty patients (12 females, 18 males) met the inclusion criteria, and 17 patients were excluded from the study due to missing or incomplete data. There were 20 group I patients (LFI+BSSO regardless of genioplasty) and 10 group II patients (BSSO regardless of genioplasty). The average time of CSP for group I was 385 ± 7.8 min, and that for group II was 195 ± 8.33 min. The time reduction rate of VSP compared with CSP was 62.8% in group I and 41.5% in group II. On the other hand, there was no statistically significant cost reduction. Conclusions: The time investment in VSP in this study was significantly smaller than that in CSP, and the difference was greater in group I than in group II.

Effect of Grape Seed Proanthocyanidins on Tumor Vasculogenic Mimicry in Human Triple-negative Breast Cancer Cells

  • Luan, Yun-Yan;Liu, Zi-Min;Zhong, Jin-Yi;Yao, Ru-Yong;Yu, Hong-Sheng
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.2
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    • pp.531-535
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    • 2015
  • Vasculogenic mimicry (VM) refers to the unique ability of highly aggressive tumor cells to mimic the pattern of embryonic vasculogenesis, which was associated with invasion and metastasis. The grape seed proanthocyanidins (GSPs) had attracted much attention as a potential bioactive anti-carcinogenic agent. However, GSPs regulation of VM and its possible mechanisms in a triple-negative breast cancer cells (TNBCs) remain not clear. Therefore, we examined the effect of GSPs on VM information in HCC1937 cell model. In this study, we identified the VM structure via the three-dimensional (3D) matrix in vitro. Cell viability was measured using the CCK8 assay. The effects of GSPs on human triple-negative breast cancer cells (TNBCs) HCC1937 in terms of related proteins of VM information were determined using western blot analysis. In vitro, the tubular networks were found in highly invasive HCC1937 cells but not in the non-invasive MCF-7 cells when plated on matrigel. The number of vascular channels was significantly reduced when cells were exposed in GSPs ($100{\mu}g$/ml) and GSPs ($200{\mu}g/mL$) groups (all p<0.001). Furthermore, we found that treatment with GSPs promoted transition of the mesenchymal state to the epithelial state in HCC1937 cells as well as reducing the expression of Twist1 protein, a master EMT regulator.GSPs has the ability to inhibit VM information by the suppression of Twist1 protein that could be related to the reversal of epithelial-to-mesenchymal (EMT) process. It is firstly concluded that GSPs may be an p otential anti-VM botanical agent for human TNBCs.

Structures and Magnetic Properties of Monomeric Copper(II) Bromide Complexes with a Pyridine-Containing Tridentate Schiff Base

  • Kang, Sung Kwon;Yong, Soon Jung;Song, Young-Kwang;Kim, Young-Inn
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3615-3620
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    • 2013
  • Two novel copper(II) bromide complexes with pyridine containing Schiff base ligands, $Cu(pmed)Br_2$ and $Cu(pmed)Br_2$ where pmed = N'-((pyridin-2-yl)methylene)ethane-1,2-diamine (pmed) and dpmed = N,N-diethyl-N'-((pyridin-2-yl)methylene)ethane-1,2-diamine (dpmed) were synthesized and characterized using X-ray single crystal structure analysis, optical and magnetic susceptibility measurements. Crystal structural analysis of $Cu(pmed)Br_2$ showed that the copper(II) ion has a distorted square-pyramidal geometry with the trigonality index of ${\tau}=0.35$ and two intermolecular hydrogen bonds, which result in the formation of two dimensional networks in the ab plane. On the other hand, $Cu(pmed)Br_2$ displayed a near square-pyramidal geometry with the value of ${\tau}=0.06$. In both compounds, the NNN Schiff base and one Br atom occupy the basal plane, whereas the fifth apical position is occupied by the other Br atom at a greater Cu-Br apical distance. The reported complexes show $g_{\mid}$ > $g_{\perp}$ > 2.0023 with a $d_{x2-y2}$ ground state and a penta-coordinated square pyramidal geometry. Variable temperature magnetic susceptibility measurements showed that the developed copper(II) complexes follow the Curie-Weiss law, that is there are no magnetic interactions between the copper(II) ions since the Cu--Cu distance is too far for magnetic contact.

Comparative Study of the Surface Roughness of Working Models Fabricated by Dental CAD/CAM Scannable Stone and Type IV Gypsum Products (치과용 캐드캠 스캔전용 석고와 Type IV 석고로 제작된 작업모형의 표면조도 비교 연구)

  • Kim, Sa-Hak;Kim, Jae-Hong
    • Journal of dental hygiene science
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    • v.14 no.4
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    • pp.455-460
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    • 2014
  • This study compared the surface roughness and surface characteristics between a type IV stone and scannable stone. Materials used were a type IV stone, two different kind of scannable stone. Ten specimens per experimental group were prepared according to manufacturer's direction. Surface roughness of specimen was measured using profilometer. The measurement was based on the standard of Japanese Industrial Standards 1994. The mean and standard deviations of each reference point were analyzed using one-way ANOVA and Scheff$\acute{e}$ post hoc test. The program used to handle statistical analysis was SPSS 20.0 and the significance level was set at 0.05. The difference of surface roughness was statistically significant in order of Scannable Stone $10.07{\pm}0.02{\mu}m$, Scannable Stone $20.08{\pm}0.03{\mu}m$, Type IV $0.10{\pm}0.04{\mu}m$. These results will have to be confirmed in further clinical application researches.