• Tissue Engineering and Regenerative Medicine
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• v.19
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• pp.1099-1111
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• 2022

Background: Bone marrow-derived mesenchymal stem cells (BMSCs) and bone morphogenetic protein-2 (BMP-2) have been studied for bone repair because they have regenerative potential to differentiate into osteoblasts. The development of injectable and in situ three-dimensional (3D) scaffolds to proliferate and differentiate BMSCs and deliver BMP-2 is a crucial technology in BMSC-based tissue engineering. Methods: The proliferation of mouse BMSCs (mBMSCs) in collagen/poly-γ-glutamic acid (Col/γ-PGA) hydrogel was evaluated using LIVE/DEAD and acridine orange and propidium iodide assays. In vitro osteogenic differentiation and the gene expression level of Col/γ-PGA(mBMSC/BMP-2) were assessed by alizarin red S staining and quantitative reverse-transcription polymerase chain reaction. The bone regeneration effect of Col/γ-PGA(mBMSC/BMP-2) was evaluated in a mouse calvarial bone defect model. The cranial bones of the mice were monitored by micro-computed tomography and histological analysis. Results: The developed Col/γ-PGA hydrogel showed low viscosity below ambient temperature, while it provided a high elastic modulus and viscous modulus at body temperature. After gelation, the Col/γ-PGA hydrogel showed a 3D and interconnected porous structure, which helped the effective proliferation of BMSCs with BMP-2. The Col/γ-PGA (mBMSC/BMP-2) expressed more osteogenic genes and showed effective orthotopic bone formation in a mouse model with a critical-sized bone defect in only 3-4 weeks. Conclusion: The Col/γ-PGA(mBMSC/BMP-2) hydrogel was suggested to be a promising platform by combining collagen as a major component of the extracellular matrix and γ-PGA as a viscosity reducer for easy handling at room temperature in BMSC-based bone tissue engineering scaffolds.

• Analytical Science and Technology
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• v.18 no.5
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• pp.381-385
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• 2005

The title compound, $\{H_2NCH_2CH_2NH_2CH_2CH_2\}\{Cu(H_2O)_6\}(SO_4)_2$, I, has been synthesized under solvo/hydrothermal conditions and their crystal structure analyzed by X-ray single crystallography. Compound I crystallizes in the monoclinic system, $P2_1/n$ space group with a = 6.852(1), b = 10.160(2), $c=11.893(1){\AA}$, ${\beta}=92.928(8)^{\circ}$, $V=826.9(2){\AA}^3$, Z = 2, $D_x=1.815g/cm^3$, $R_1=0.031$ and ${\omega}R_2=0.084$. The crystal structure of the piperazine templated Cu(II)-sulfate demonstrate zero-dimensional compound constituted by doubly protonated piperazine cations, hexahydrated copper cations and sulfate anions. The central Cu atom has a elongated octahedral coordination geometry. The crystal structure is stabilized by three-dimensional networks of the intermolecular $O_{water}-H{\cdots}O_{sulfate}$ and $N_{pip}-H{\cdots}O_{sulfate}$ hydrogen bonds between the water molecules and sulfate anions and protonated piperazine cations. Based on the results of thermal analysis, the thermal decomposition reaction of compound I was analyzed to have three distinctive stages.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.5
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• pp.433-441
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• 2009

Generally, the events in nature have multi-disciplinary characteristics. To solve this problems, these days loosely coupled methods are widely applied because of advantage of solvers which are already developed and well proved. Those solvers use different mesh system, so transformation and mapping of data are vital in the field of fluid-structure interaction(FSI). In this paper, the interpolation of deformation which is used globally and compactly supported radial basis functions(RBF), and mapping of force which use principle of virtual work are examined for computing time and accuracy to compare ability with simple 3-D problem. As the results, interpolation scheme of compactly supported radial basis functions are useful to interpolation and mapping for large-scale airplane in FSI with a k-dimensional tree(kd-tree) which is a space-partitioning data structure for organizing points in a k-dimensional space.

• Transactions of the Korean Society of Mechanical Engineers
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• v.11 no.1
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• pp.82-87
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• 1987

In this study a method of shape optimization is applied to two dimensional heat transfer system. For this the optimization problem is defined in a functional form including cost, constraints and the system governing equation. Then the material derivative concept in continuum mechanics and the adjoint variable method are employed for the shape design sensitivity analysis. With the sensitivity analysis results, an optimum is sought with the gradient projection optimization algorithm. The two dimensional isoparametric finite elements are used for accurate analysis and sensitivity calculations. The above method is employed to find the boundary shape to achieve a desired temperature distribution along a segment of the boundary subject to the maximum area constraint.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.38 no.5
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• pp.271-275
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• 2012

Objectives: This study evaluated a range of fixation methods to determine which is best for the postoperative stabilization of a mandibular osteotomy using three-dimensional finite element analysis of the stress distribution on the plate, screw and surrounding bone and displacement of the lower incisors. Materials and Methods: The model was generated using the synthetic skull scan data, and the surface model was changed to a solid model using software. Bilateral sagittal split ramus osteotomy was performed using the program, and 8 different types of fixation methods were evaluated. A vertical load of 10 N was applied to the occlusal surface of the first molar. Results: In the case of bicortical screws, von-Mises stress on the screws and screw hole and deflection of the lower central incisor were minimal in type 2 (inverted L pattern with 3 bicortical repositioning screws). In the case of plates, von-Mises stress was minimal in type 8 (fixation 5 mm above the inferior border of the mandible with 1 metal plate and 4 monocortical screws), and deflection of the lower central incisor was minimal in types 6 (fixation 5 mm below the superior border of the mandible with 1 metal plate and 4 monocortical screws) and 7 (fixation 12 mm below the superior border of the mandible with 1 metal plate and 4 monocortical screws). Conclusion: Types 2 and 6 fixation methods provide better stability than the others.

• The Mathematical Education
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• v.48 no.1
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• pp.1-20
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• 2009

The study was to investigate an effect of students' learning for enhancing spatial ability, using a geometric manipulative recently designed. A mixed methodology was chosen to achieve the purpose of the study. To find students' achievement, 152 of the 8th graders in Kyunggi Do participated in data collection. At the same time. students' performance of the class was videotaped and analyzed to see students' responses, The results showed that the effect of using the manipulative was statistically significant at level, p<.05 to enhance the spatial ability. Specifically, in comparison of each component. spatial orientation was more effective than spatial visualization. In the spatial orientation, the part of field was more effective than the reorganized whole. It showed that students were given more opportunities to find mathematical properties and relations between 2nd and 3rd-dimensional figures through their intuitive observation, and also the manipulative helped the students find the property of the part of field because it gave an easy way to manipulate the property of the find parts of whole which was composed of the frame of the solid figures without surfaces. In using the manipulative, students were very flexible in finding the number of plane figures, but the relations between the 2nd and 3rd dimensional figures need to be clearly guided in consideration of the characteristics of the manipulative, based on the definitions of geometric properties(cf. points can make lines, not surfaces directly).

• Journal of Sensor Science and Technology
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• v.12 no.6
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• pp.249-257
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• 2003

The bio-telemetry technologies, that use the wireless miniaturized telemetry module implanted in the human body and transmits several biomedical signal from inside to outside of the body, have been expected to solve the problem such as the patient's inconvenience and the limit for diagnosis. In the case of transceiver system using the wireless RF transmission method, the method of three-dimensional localization for implantable miniaturized telemetry module is necessary to detect the exact position of disease. A new method for three-dimensional localization using small loop antenna in the implantable miniaturized telemetry module was proposed in this paper. We proposed a method that can accurately determine the position of telemetry module by analyzing the differences in the strength of signal, which is received at each of the small size RF receiver array installed on the body surface.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.6
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• pp.714-725
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• 2003

The theory of the current-type electromagnetic flowmeter for a high temporal resolution was developed for two-phase flow measurements. To predict the output of the current-type flowmeter, the three-dimensional virtual potential distribution C and the newly introduced flow pattern coefficient f were derived and computed. The output of flowmeter depends on the liquid conductivity (sensitive to temperature) and flow configurations of the two-phase flow with the sinusoidal excitation over 100 Hz. The flow pattern coefficient was specially devised to separate the dependency on the flow configuration of the two-phase flow from that on the liquid conductivity which can be expressed with the calibration of single-phase flow. Using the finite difference method, the three-dimensional virtual potential distributions were computed for the electrode of finite size. By taking derivative of the virtual potential, the weight functions were evaluated and compared with existing analytic series solution for the point-electrode. There was a reasonable correspondence between the present and existing results. In addition, the flow pattern coefficients were evaluated for annular flows with various film thicknesses, and compared with the experimental results by the impedance spectroscopy. The numerical results agreed well with the experimental data.

• Atmosphere
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• v.30 no.2
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• pp.169-181
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• 2020

We conducted a study on the impact of observation station density; this was done in order to enable the accurate estimation of spatial meteorological variables. The purpose of this study is to help operate an efficient observation network by examining distributions of temperature, relative humidity, and wind speed in a test area of a three-dimensional meteorological observation project in the Yeongdong region in 2019. For our analysis, we grouped the observation stations as follows: 41 stations (for Step 4), 34 stations (for Step 3), 17 stations (for Step 2), and 10 stations (for Step 1). Grid values were interpolated using the kriging method. We compared the spatial accuracy of the estimated meteorological grid by using station density. The effect of increased observation network density varied and was dependent on meteorological variables and weather conditions. The temperature is sufficient for the current weather observation network (featuring an average distance about 9.30 km between stations), and the relative humidity is sufficient when the average distance between stations is about 5.04 km. However, it is recommended that all observation networks, with an average distance of approximately 4.59 km between stations, be utilized for monitoring wind speed. In addition, this also enables the operation of an effective observation network through the classification of outliers.

• Journal of The Korean Astronomical Society
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• v.34 no.4
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• pp.309-311
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• 2001

Strong thermal X-ray emission, called Galactic Ridge X-ray Emission, is observed along the Galactic plane (Koyama et al. 1986). The origin of hot ($\~$7 keV) component of GRXE is not known, while cool ($\~$0.8 keV) one is associated with supernovae (Kaneda et al. 1997, Sugizaki et al. 2001). We propose a possible mechanism to explain the origin; locally strong magnetic fields of $B_{local}\;\~30{\mu}G$ heat interstellar gas to $\~$7 keV via magnetic reconnection (Tanuma et al. 1999). There will be the small-scale (< 10 pc) strong magnetic fields, which can be observed as $(B)_{obs} \;\~3{\mu}G$ by integration of Faraday Rotation Measure, if it is localized by a volume filling factor of f $\~$ 0.1. In order to examine this model, we solved three-dimensional (3D) resistive magnetohydrodynamic (MHD) equations numerically to examine the magnetic reconnect ion triggered by a supernova shock (fig.l). We assume that the magnetic field is Bx = 30tanh(y/20pc) $\mu$G, By = Bz = 0, and the temperature is uniform, at the initial condition. We put a supernova explosion outside the current sheet. The supernova-shock, as a result, triggers the magnetic reconnect ion, and the gas is heatd to > 7 keV. The magnetic reconnect ion heats the interstellar gas to $\~$7 keV in the Galactic plane, if it occurs in the locally strong magnetic fields of $B_{local}\;\~30{\mu}G$. The heated plasma is confined by the magnetic field for $\~10^{5.5} yr$. The required interval of the magnetic reconnect ions (triggered by anything) is $\~$1 - 10 yr. The magnetic reconnect ion will explain the origin of X-rays from the Galactic ridge, furthermore the Galactic halo, and clusters of galaxies.