• BMB Reports
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• v.37 no.3
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• pp.304-313
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• 2004

Three-dimensional (3D) models for the 65-kDa activated Cry4A and Cry4B $\delta$-endotoxins from Bacillus thuringiensis subsp. israelensis that are specifically toxic to mosquito-larvae were constructed by homology modeling, based on atomic coordinates of the Cry1Aa and Cry3Aa crystal structures. They were structurally similar to the known structures, both derived 3D models displayed a three-domain organization: the N-terminal domain (I) is a seven-helix bundle, while the middle and C-terminal domains are primarily comprise of anti-parallel $\beta$-sheets. Circular dichroism spectroscopy confirmed the secondary structural contents of the two homology-based Cry4 structures. A structural analysis of both Cry4 models revealed the following: (a) Residues Arg-235 and Arg-203 are located in the interhelical 5/6 loop within the domain I of Cry4A and Cry4B, respectively. Both are solvent exposed. This suggests that they are susceptible to tryptic cleavage. (b) The unique disulphide bond, together with a proline-rich region within the long loop connecting ${\alpha}4$ and ${\alpha}5$ of Cry4A, were identified. This implies their functional significance for membrane insertion. (c) Significant structural differences between both models were found within domain II that may reflect their different activity spectra. Structural insights from this molecular modeling study would therefore increase our understanding of the mechanic aspects of these two closely related mosquito-larvicidal proteins.

• The Journal of Korean Society for Radiation Therapy
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• v.33
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• pp.71-78
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• 2021

Purpose: To evaluate the inter-fractional position and respiratory reproducibility of lung and liver tumors using pressure conserving type(P-type) abdominal compressor in volumetric modulated arc therapy(VMAT). Materials and methods: Six lung cancer patients and three liver cancer patients who underwent VMAT using a P-type abdominal compressor were included in this study. Cone-beam computed tomography(CBCT) images were acquired before each treatment and compared with planning CT images to evaluate the inter-fractional position reproducibility. The position variation was defined as the difference of position shift values between target matching and bone matching. 4-dimensional cone-beam computed tomography(4D CBCT) images were acquired weekly before treatment and compared with planning 4DCT images to evaluate the inter-fractional respiratory reproducibility. The respiratory variation was calculated by the magnitude of excursions by breathing. Results: The mean ± standard deviation(SD) of overall position variation values, 3D vector in the three translational directions were 1.1 ± 1.4 mm and 4.5 ± 2.8 mm for the lung and liver, respectively. The mean ± SD of respiratory variation values were 0.7 ± 3.4 mm (p = 0.195) in the lung and 3.6 ± 2.6 mm (p < 0.05) in the liver. Conclusion: The use of P-type compressor in lung and liver VMAT was effective for stable control of inter-fractional position and respiratory variation by reproduction of abdominal compression. Appropriate PTV margin must be considered in treatment planning, and image guidance before each treatment are required in order to obtain more stable reproducibility

• Journal of the Korean Geotechnical Society
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• v.37 no.5
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• pp.47-63
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• 2021

Recently, the demand for three-dimensional (3D) underground maps from the perspective of digital twins and the demand for linkage utilization are increasing. However, the vastness of national geotechnical survey data and the uncertainty in applying geostatistical techniques pose challenges in modeling underground regional geotechnical characteristics. In this study, an optimal learning model based on multi-layer perceptron (MLP) was constructed for 3D subsurface lithological and geotechnical classification in Seoul, South Korea. First, the geotechnical layer and 3D spatial coordinates of each borehole dataset in the Seoul area were constructed as a geotechnical database according to a standardized format, and data pre-processing such as correction and normalization of missing values for machine learning was performed. An optimal fitting model was designed through hyperparameter optimization of the MLP model and model performance evaluation, such as precision and accuracy tests. Then, a 3D grid network locally assigning geotechnical layer classification was constructed by applying an MLP-based bet-fitting model for each unit lattice. The constructed 3D geotechnical layer map was evaluated by comparing the results of a geostatistical interpolation technique and the topsoil properties of the geological map.

• Journal of Applied Biological Chemistry
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• v.53 no.4
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• pp.225-231
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• 2010

To understand the inhibitory activity with changing hydroxyl substituents ($R_l-R_9$) of polyhydroxy substituted 2-phenyl-l,4-benzopyrone analogues (1-25) against tyrosinase (PDB ID: oxy-form; 1WX2), molecular docking and the three dimensional quantitative structure-activity relationships (3D-QSARs: Comparative molecular field analysis (CoMFA) & Comparative molecular similarity indices analysis (CoMSIA)) were studied quantitatively. The statistically best models were CoMFA 1 and CoMSIA 1 model from the results. The optimized CoMSIA 1 model with the sensitivity of the perturbation and the prediction produced ($dq^2'/dr_{yy'}^2$=1.009 & $q^2$=0.51l) by a progressive scrambling analysis were not dependent on chance correlation. The inhibitory activities with optimized CoMSIA 1 model were dependent upon electrostatic factor (51.4%) of substrate molecules. Contour mapping the 3D-QSAR models to the active site of tyrosinase provides new insight into the interaction between tyrosinase as receptor and 2-phenyl-l,4-benzopyrone analogues as inhibitor. Therefore, the results will he able to apply to the optimization of a new potent tyrosinase inhibitors.

• Korean Journal of Applied Biomechanics
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• v.15 no.4
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• pp.181-189
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• 2005

To know the proper impact posture and changes for the various clubs, changes of impact variables according to the change of golf club length was investigated. Swing motions of three male low handicappers including a professional were taken using two high-speed video cameras. Four clubs iron 7, iron 5, iron 3 and driver (wood 1) were selected for this experiment. Three dimensional motion analysis techniques were used to get the kinematical variables. Mathcad and Kwon3D motion analysis program were used to analyze the position, distance and angle data in three dimensions. Major findings of this study were as follows. 1. Lateral position of the head remained more right side of the target up to 3.5cm compared to the setup as the length of the club increased. 2. Left shoulder raised up to 5cm and right shoulder lowered up to 2.5cm compared to setup. The shoulder line opened slightly (maximum 11 degrees) to the target line. 3. Forward lean angle of the trunk decreased up to 4 degrees (more erected) compared to setup. 4. Side lean angle of the trunk increased compared to setup and increased up to 16 degrees as the club length increased. 5. The pelvis moved to the target line direction horizontally and opened up to 31 degrees. Right hip moves laterally to the grip position at the setup. 6. Flexion of the left leg maintained almost constantly but the right leg flexed up to 11 degrees compared to setup. 7. Left arm is straightened but the right arm flexed about 20degrees compared to straight. 8. Center of the shoulders were in front of the knees and toes of the feet. 9. Hands moved to the left (8.7cm), forward (5.7cm) and upward (11.6cm) compared to the setup. This is because of the rotation of pelvis and shoulders. 10. Shaft angle to the ground was smaller than the lie angle of the clubs but it increased close to the lie of the clubs at impact.

• Journal of the Korean GEO-environmental Society
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• v.22 no.4
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• pp.15-23
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• 2021

Various micropiles have been developed through research related to micropiles, which have been carried out with the increased use of micropiles. Among the micropile construction methods being developed, the triaxial micropile (tmp), which is recently developed for the purpose of increasing the horizontal bearing capacity (seismic resistance), is representative. The three-axis micropile has the advantage of a method that can resist horizontal load more effectively because three micropiles installed inclined on each axis resist horizontal load. However, there is a problem in effectively using this pile method due to insufficient research on the support characteristics of the triaxial group micropile. In order to effectively utilize the triaxial group micropile (tmp), it is required to evaluate the bearing capacity for the factors that affect the horizontal bearing capacity of the pile. Therefore, in this study, field horizontal loading Tests were performed for each load direction, field loading Tests were verified through three-dimensional finite element analysis, behavioral characteristics of triaxial micropiles were evaluated, and appropriate horizontal bearing capacity was analyzed in consideration of horizontal load directions.

• Journal of Broadcast Engineering
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• v.17 no.3
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• pp.437-446
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• 2012

A face detection algorithms using two-dimensional (2-D) intensity or color images have been studied for decades. Recently, with the development of low-cost range sensor, three-dimensional (3-D) information (i.e., depth image that represents the distance between a camera and objects) can be easily used to reliably extract facial features. Most people have a similar pattern of 3-D facial structure. This paper proposes a face detection method using intensity and depth images. At first, adaboost algorithm using intensity image classifies face and nonface candidate regions. Each candidate region is divided into $5{\times}5$ blocks and depth values are averaged in each block. Then, $5{\times}5$ block rank pattern is constructed by sorting block averages of depth values. Finally, candidate regions are classified as face and nonface regions by matching the constructed depth map based block rank patterns and a template pattern that is generated from training data set. For template matching, the $5{\times}5$ template block rank pattern is prior constructed by averaging block ranks using training data set. The proposed algorithm is tested on real images obtained by Kinect range sensor. Experimental results show that the proposed algorithm effectively eliminates most false positives with true positives well preserved.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.2B
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• pp.113-121
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• 2012

When a heavy rain brings flooding, a high turbid water is flowing into a reservoir. In this study, the effectiveness of the intake structures for the selective withdrawal from the various levels of a stratified reservoir was evaluated by the field experiments and the numerical modeling of the three-dimensional approaching flows. The temperature, the turbidity, and the velocity fields for the selective withdrawal were measured using both YSI6600EDS and YSI6600ADV, respectively. A threedimensional model, FLOW-3D, was used to predict the performance of the intake tower in Imha reservoir. The comparisons of the vertical velocity field showed a good agreement with the field measurements. The efficiency of the turbid-water elimination of the selective withdrawal method from low levels was higher up to 46% than that of the surface withdrawal. From the analysis of the numerical simulation, the efficiency of turbidity elimination increased by 10% for the selective withdrawal from middle levels, and by 30% from low levels. These results showed that the selective withdrawals from middle and low levels are more effective than the surface-water intake. The similar results were obtained by the one-dimensional model, SELECT, which is much more computationally time-efficient.

• Journal of the Korean Geotechnical Society
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• v.17 no.3
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• pp.41-50
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• 2001

본 연구는 모래사면의 언덕근처에 설치된 짧은 말뚝의 수평하중의 영향에 관한 것이다. 3차원 탄소성 유한요소법해석과 실내 모형실험의 결과를 비교하였다. 경사 30$^{\circ}$의 사면에 시공된 짧은 말뚝의 특성을 파악하기 위해, 사면언덕에서 모형말뚝까지의 거리를 3종류로 상이하게 하여, 모형실험을 실시하였다. 사용된 모래의 지반특성은 배수조건하의 삼축압축실험으로 결정하였다. 동시에 3차원 탄소성 유한요소법에 의한 수치해석결과와 모형실험결과를 비교하였다. 본 유한요소법의 해석에 있어서 모래지반을 탄성완전소성모델(Elastic-perfectly plastic model)로 가정하여, 파괴기준으로 Mohr-Coulomb 식과 소성 포텐셜에 대해서는 Drucker-Prager 식을 적용한 MC-DP 모델로 하였다. 이러한 MC-DP 모델의 구성식은 유한요소법에서 있어 계산치의 수렴에 유익하다. 3차원 탄소성 유한요소법에 의한 수치해석이 사질토 사면의 언덕 부근에 설치된 단하의 수평거동에 대한 파악에 유효하다는 것을 확인하였다.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.41 no.4
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• pp.101-112
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• 2004

In this paper, we developed a numerical analysis model by using ADI-FDTD method to analyze three-dimensional interconnect structure. We discretized maxwell's curl equation by using ADI-FDTD. Using ADI-FDTD method, a sampler circuit designed from 3.3 V CMOS technology is simplified to 3-metal line structure. Using this simplified structure, the time delay and signal distortion of complex interconnects are investigated. As results of simulation, 5∼10 ps of delay time and 0.1∼0.2 V of signal distortion are measured. As demonstrated in this paper, the full-wave analysis using ADI-FDTD exhibits a promise for accurate modeling of electromagnetic phenomena in high-speed VLSI interconnect.