• Title/Summary/Keyword: 3A

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Molecular Cloning and Characterization of a Flavanone-3-hydroxylase Gene from Rubus occidentalis L.

  • Lee, Seung Sik;Lee, Eun Mi;An, Byung Chull;Barampuram, Shyamkumar;Kim, Jae-Sung;Cho, Jae-Young;Lee, In-Chul;Chung, Byung Yeoup
    • Journal of Radiation Industry
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    • v.2 no.3
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    • pp.121-128
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    • 2008
  • Flavanone-3-hydroxylase (F3H) is one of the key enzymes for the biosynthesis of flavonals, anthocyanins, catechins and proanthocyanins. F3H catalyzes the $3{\beta}$-hydroxylation of (2S)-flavonones to form (2R, 3R)-dihydroflavonols. In this report, we isolated a full-length cDNA of RocF3H from black raspberry (Rubus occidentalis L.) using a reverse transcriptase-PCR and rapid amplification of the cDNA ends (RACE)-PCR. The full-length cDNA of RocF3H contains a 1,098 bp open reading frame (ORF) encoding a 365 amino acid protein with a calculated molecular weight of about 41.1 kDa and isoelectric point (pI) of 5.45. The genomic DNA analysis revealed that the RocF3H gene had three exons and two introns. Comparison of the deduced amino acid sequence of the RocF3H with other F3Hs revealed that the protein is highly homologous with various plant species. The conserved amino acids ligating the ferrous iron and the residues participating in the 2-oxoglutarate binding (R-X-S) were found in RocF3H at the similar positions to other F3Hs. Southern blot analysis indicated that RocF3H exist a multi-gene family. The isolation of RocF3H gene will be helpful to further study the role of F3H gene in the biosynthesis of flavonoids in R. occidnetalis.

Development of 3D Underground Utilities Processing and Partial Update Automation Technology - Focused on 3D Underground Geospatial Map - (3차원 지하시설물 가공 및 부분갱신 자동화 기술개발 - 지하공간통합지도 중심으로 -)

  • LEE, Min-Kyu;CHOI, Sung-Sik;JEON, Heung-Soo;KIM, Sung-Su
    • Journal of the Korean Association of Geographic Information Studies
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    • v.23 no.4
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    • pp.1-15
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    • 2020
  • As cities expand and underground utilities construction projects increase, there is an urgent need for a technology capable of analyzing the underground utilities network in 3D. Since 2015, 3D Underground Geospatial Map project, that has been integrating 15 types of underground information such as underground utilities, underground structures, and ground information, is in progress in S. Korea. However, the construction of 3D underground facilities is currently based on manual work and the logic for building a 3D model is very complicated. And it takes a lot of time and cost to process millions of large amounts of data per local governments. By presenting a framework on the processing and partial updating of the 3D underground utilities model, this paper aims to establish a plan to quickly build a 3D underground utility model at a minimum cost. The underground utilities processing and partial update automation technologies developed in this study are expected to be immediately applied to the 3D Underground Geospatial Map project.

Crude Extract and Solvent-Partitioned Fractions of the Halophyte Atriplex gmelinii Inhibit Adipogenesis in 3T3-L1 Preadipocytes (3T3-L1 지방전구세포에서 염생식물 Atriplex gmelinii의 조추출물과 용매 분획물의 지방세포분화 억제)

  • Jung Im Lee;Jung Hwan Oh;Chang-Suk Kong;Youngwan Seo
    • Journal of Korean Medicine for Obesity Research
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    • v.23 no.2
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    • pp.69-77
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    • 2023
  • Objectives: Atriplex gmelinii C. A. Meyer is a halophyte belonging to the Chenopodiaceae family, and its young leaves and stems are used as fodder for livestock. The aim of the present study was to investigate the effects of A. gmelinii extract and its solvent fractions on lipid accumulation during adipogenesis of 3T3-L1 preadipocytes. Methods: The samples of A. gmelinii were separately extracted using methylene chloride and methanol. Subsequently, they were combined to formulate the initial extract, which was then partitioned based on polarity to prepare solvent fractions. Oil Red O staining was employed to measure lipid accumulation during the differentiation of 3T3-L1 preadipocytes. To verify cytotoxicity in 3T3-L1 cells, MTT assays were conducted. The expression levels of transcription factors in 3T3-L1 preadipocytes were measured through Western blotting analysis. Results: At 50 ㎍/mL, treatment of A. gmelinii extract and its solvent fractions during the differentiation of 3T3-L1 preadipocytes significantly diminished lipid accumulation with no noteworthy cytotoxicity on cell viability. Additionally, when investigating the biochemical pathways that underlie the prevention of lipid accumulation using solvent fractions, it was found that the n-BuOH and n-hexane fractions significantly decreased the expression of key transcription factors involved in the generation of fat, such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and sterol regulatory element-binding protein-1c (SREBP1c). Conclusions: These findings indicate that A. gmelinii can effectively reduce the accumulation of fat in 3T3-L1 adipocytes, making it a potentially valuable material for mitigating and preventing obesity.

Fast 3D mesh generation using projection for line laser-based 3D Scanners (라인 레이저 기반 3차원 스캐너에서 투영을 이용한 고속 3D 메쉬 생성)

  • Lee, Kyungme;Yoo, Hoon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.3
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    • pp.513-518
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    • 2016
  • This paper presents a fast 3D mesh generation method using projection for line laser-based 3D scanners. The well-known method for 3D mesh generation utilizes convex hulls for 4D vertices that is converted from the input 3D vertices. This 3D mesh generation for a large set of vertices requires a lot of time. To overcome this problem, the proposed method takes (${\theta}-y$) 2D depth map into account. The 2D depth map is a projection version of 3D data with a form of (${\theta}$, y, z) which are intermediately acquired by line laser-based 3D scanners. Thus, our 2D-based method is a very fast 3D mesh generation method. To evaluate our method, we conduct experiments with intermediate 3D vertex data from line-laser scanners. Experimental results show that the proposed method is superior to the existing method in terms of mesh generation speed.

Synthesis of the Nano-sized SrAl2O4 Phosphors by Wet Processing and its Photoluminescence Properties (SrAl2O4계 축광재료의 습식공정에 의한 나노분말 합성 및 발광특성)

  • Kim, Jung-Sik
    • Journal of the Korean Ceramic Society
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    • v.45 no.8
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    • pp.477-481
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    • 2008
  • $Eu^{2+}$ and $Dy^{3+}$ co-doped strontium aluminate, $SrAl_2O_4$ long phosphorescent phoshor was fabricated and its photoluminescence was characterized. The phosphor, $SrAl_2O_4:Eu^{2+},Dy^{3+}$ was synthesized by a coprecipitation in which metal salts of $Sr(NO_3)_2$, $Al(NO_3)_3{\cdot}9H_2O$, were dissolved in $(NH_4)_2CO_3$ solution with adding $Eu(NO_3)_3{\cdot}5H_2O$ and $Dy(NO_3)_3{\cdot}5H_2O$ as a activator and co-activator, respectively. The coprecipitated products were separated from solution, washed, and dried in a vacuum dry oven. The dried powders were then mixed with 3 wt% $B_2O_3$ as a flux and heated at $800{\sim}1400^{\circ}C$ for 3 h under the reducing ambient atmosphere of 95%Ar+$5%H_2$ gases. For the synthesized $SrAl_2O_4:Eu^{2+},Dy^{3+}$, properties of photoluminescence such as emission, excitation and decay time were examined. The emission intensity increased as the annealing temperature increased and showed a maximum peak intensity at 510 nm with a broad band from $400{\sim}650\;nm$. Monitored at 520 nm, the excitation spectrum showed a maximum peak intensity at $315{\sim}320\;nm$ wavelength with a broad band from $200{\sim}500\;nm$ wavelength. The decay time of $SrAl_2O_4:Eu^{2+},Dy^{3+}$ increased as the annealing temperature increased.

A Parallel Algorithm for 3D Geographic Information System (3차원 공간정보 시스템을 위한 병렬 알고리즘)

  • Jo, Jeong-U;Kim, Jin-Seok
    • The KIPS Transactions:PartA
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    • v.9A no.2
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    • pp.217-224
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    • 2002
  • Many systems handle 3D-image were used. High-performance computer systems and techniques of compressing images to handle 3D-image were used. But there will be cost Problems, if GIS system is implemented, using the high-performance system. And if GIS system is implemented, using the techniques of compressing images, there will be some loss of a image. It will take a long processing time to handle 3D-images using a general PC because the size of 3D-image files are very huge. The parallel algorithm presented in the paper can improve speed to handle 3D-image using parallel computer system. The system uses the method of displacing images from nodes to screens, dividing a 3D-image into multiple sub images on multiple nodes. The performance of the presented algorithm showers improving speed by experiments.

Mobile Panorama System via 3D Model Reconstruction (3차원 모델 재구성을 통한 모바일 파노라마 시스템)

  • Kim, Jin-Hee;Choy, Yoon-Chul;Han, Tack-Don;Lim, Soon-Bum
    • Journal of Korea Multimedia Society
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    • v.14 no.8
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    • pp.1094-1107
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    • 2011
  • We can use panorama systems or image based modeling systems when we want to make 3D space model and look around result. Panorama systems make 3D model to stitch images and map cylinder or cube. The structure of 3D model, made by panorama system, is not same as structure of a real room, so user can't infer a real structure. Typically, Image based modeling systems work on a desktop computer. That makes it difficult to reconstruct 3D model in real time and take long time for processing. In this paper, we propose a 3D panorama modeling system that uses images on a mobile device. This system reconstructs a 3D space model, similar with a real room in real time, from multiple images captured part of rooms. Using this system, user can reconstruct various shape of space and look around a 3D space model.

Hardware Implementation of FPGA-based Real-Time Formatter for 3D Display (3D 디스플레이를 위한 FPGA-기반 실시간 포맷변환기의 하드웨어 구현)

  • Seo Young-Ho;Kim Dong-Wook
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.9 no.5
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    • pp.1031-1038
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    • 2005
  • In this paper, we propose real-time 3D image converting architecture by a unit of pixel for 2D/3D compatible PC and LCD of cellular phone with parallax burier, and implement a system for overall display operation after designing a circuit based on FPGA. After digitizing anolog image signal from PC, we recompose it to 3D image signal according to input image type. Since the architecture which rearranges 2D image to 3D depends on parallax burier, we use interleaving method which mixes pixels by a unit of R, G, and B cell. The propose architecture is designed into a circuit based on FPGA with high-speed memory access technique and use 4 SDRAMs for high performance data storing and processing. The implemented system consists of A/D converting system, FPGA system to formatting 2D signal to 3D, and LCD panel with parallax barrier, for 3D display.

5kg $U_{3}O_{8}$ Batch Scale Mock-up Test for the Electrochemical Reduction of Spent Oxide Fuel (사용후핵연료의 전기화학적 금속전환을 위한 5kg $U_{3}O_{8}$ Batch 규모의 Mock-up 시험)

  • 오승철;허진목;홍순석;이원경;서중석;박승원
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.1 no.1
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    • pp.47-53
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    • 2003
  • An electrochemical reduction technology which can reduce the decay heat, volume, and radioactivity of spent fuel by a factor of quarter through converting oxide type spent fuel to a metallic form in a molten salt was developed and tests in a scale of g (3- 40g $U_{3}O_{8}$ batch) have been carried out by Korea Atomic Energy Research Institute. In this research, the reaction apparatus in a scale of 5kg $U_{3}O_{8}$ batch was designed and manufactured for the mock-up test to obtain design data of the apparatus which will be used for the hot test in a scale of 20kg $U_{3}O_{8}$ batch. The electrochemical reduction behavior of $U_{3}O_{8}$ was analyzed regarding the operational factors and fresh $U_{3}O_{8}$ powder was metallized with a more than 99% yield verifying the process validity of electrochemical reduction process in a kg scale.

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CAD Data Conversion to a Node-Relation Structure for 3D Sub-Unit Topological Representation (3차원 위상구조 생성을 위한 노드 - 관계구조로의 CAD 자료 변환)

  • Stevens Mark;Choi Jin-Mu
    • Journal of the Korean Geographical Society
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    • v.41 no.2 s.113
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    • pp.188-194
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    • 2006
  • Three-dimensional topological data is essential for 3D modeling and application such as emergency management and 3D network analysis. This paper reviewed current 3D topological data model and developed a method to construct 3D topological node-relation data structure from 2D computer aided design (CAD) data. The method needed two steps with medial axis-transformation and topological node-relation algorithms. Using a medial-axis transformation algorithm, the first step is to extract skeleton from wall data that was drawn polygon or double line in a CAD data. The second step is to build a topological node-relation structure by converting rooms to nodes and the relations between rooms to links. So, links represent adjacency and connectivity between nodes (rooms). As a result, with the conversion method 3D topological data for micro-level sub-unit of each building can be easily constructed from CAD data that are commonly used to design a building as a blueprint.