• Journal of Microbiology and Biotechnology
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• v.21 no.11
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• pp.1159-1165
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• 2011

Biodiesel is methyl and ethyl esters of long-chain fatty acids produced from vegetable oils or animal fats. Lipase enzymes have occasionally been used for the production of this biofuel. Recently, biodiesel production using immobilized lipase has received increased attention. Through enhanced stability and reusability, immobilized lipase can contribute to the reduction of the costs inherent to biodiesel production. In this study, methanol-tolerant lipase M37 from Photobacterium lipolyticum was immobilized using the cross-linked enzyme aggregate (CLEA) method. Lipase M37 has a high lysine content (9.7%) in its protein sequence. Most lysine residues are located evenly over the surface of the protein, except for the lid structure region, which makes the CLEA preparation yield quite high (~93%). CLEA M37 evidences an optimal temperature of $30^{\circ}C$, and an optimal pH of 9-10. It was stable up to $50^{\circ}C$ and in a pH range of 4.0-11.0. Both soluble M37 and CLEA M37 were stable in the presence of high concentrations of methanol, ethanol, 1-propanol, and n-butanol. That is, their activities were maintained at solvent concentrations above 10% (v/v). CLEA M37 could produce biodiesel from olive oil and alcohols such as methanol and ethanol. Additionally, CLEA M37 generated biodiesel via both 2-step methanol feeding procedures. Considering its physical stability and reusability, CLEA M37 may potentially be used as a catalyst in organic synthesis, including the biodiesel production reaction.

• Journal of the Korean Society of Radiology
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• v.17 no.1
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• pp.37-46
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• 2023

This study aims to evaluate the usefulness of Deep Learning Image Reconstruction (TrueFidelity, TF), the image quality of existing Filtered Back Projection (FBP) and Adaptive Statistical Iterative Reconstruction-Veo (ASIR-V) were compared. Noise, CNR, and SSIM were measured by obtaining images with doses fixed at 17.29 mGy and altered to 10.37 mGy, 12.10 mGy, 13.83 mGy, and 15.56 mGy in reconstruction techniques of FBP, ASIR-V 50%, and TF-H. TF-H has superior image quality compared to FBP and ASIR-V when the reconstruction technique change is given at 17.29 mGy. When dose changes were made, Noise, CNR, and SSIM were significantly different when comparing 10.37 mGy TF-H and FBP (p<0.05), and no significant difference when comparing 10.37 mGy TF-H and ASIR-V 50% (p>0.05). TF-H has a dose-reduction effect of 30%, as the highest dose of 15.56 mGy ASIR-V has the same image quality as the lowest dose of 10.37 mGy TF-H. Thus, Deep Learning Reconstruction techniques (TF) were able to reduce dose compared to Iterative Reconstruction techniques (ASIR-V) and Filtered Back Projection (FBP). Therefore, it is considered to reduce the exposure dose of patients.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.165-172
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• 2004

This study was carried out to examine the effects of maturation media on in vitro maturation (IVM) of porcine immature oocytes, and on subsequent in vitro fertilization (IVF) and development (IVD). Cumulus-oocyte complexes (COCs) were collected from antral follicles of porcine ovaries collected from abattoir, and were matured in vitro in modified NCSU-37 (mNCSU-37), modified NCSU-23 (mNCSU-23), or TCM-199 supplemented with 10% porcine follicular fluid (pFF). Oocytes matured in vitro, were fertilized in vitro in modified Tris-buffered medium(mTBM) with the final motile sperm concentration of 1${\times}$105 sperm/mL, and subsequently putative embryos were developed in vitro in NCSU-23. The results are as follows. 1. In the result of IVM, the rate of germinal vesicle breakdown (GVBD) and of nuclear maturation were not significantly different among the media, though numeric value of them were slightly lower in TCM-199 than in mNCSU-37 or in mNCSU-23. 2. In the result of IVF, though the rate of sperm penetration was not significantly different among the maturation media, the percentage of oocytes with male pronucleus (MPN) of ones matured in mNCSU-37 (88.0%) was significantly higher than in TCM-199 (71.1%) (p<0.05). 3. In the result of IVD, the percentage of cleaved oocytes of ones matured in mNCSU-37 (52.3%) or in mNCSU-23 (53.7%) was significantly higher than in TCM-199 (43.1%) (p<0.05), but the rate of blastocysts at day 6 was not significantly different among the maturation media, though putative embryos from oocytes matured in mNCSU-37 or in mNCSU-23 were developed more than in TCM-199. These results suggested that mNCSU-37 or mNCSU-23 was more appropriate than TCM-199 as IVM medium for porcine immature oocytes.

• Venture DIGEST
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• s.135
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• pp.37-37
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• 2009

• Journal of Korea Water Resources Association
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• v.31 no.4
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• pp.469-481
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• 1998

A point frequency analysis is carried out for the Indogyo site at the Han river using 68 annual maximun flood data for the period of 1918-1992. Computed frequency discharges using the three parameter log-normal, type-I extreme value, type-III extreme value, and Pearson type-III computed as 35,500 m3/sec and 39,000 m3/sec, respectively, 33,500 m3/sec and 37,500 m3/sec of corresponding return periods are computed when the flood control effect of the dams are taken into account. The resulting flood discharge of 37,500 m3/sec is similar to the current design flood of 37,000 m3/sec in downstream reach of Han river, so, it could be desirable to keep the the current design flood, considering the increasing tendency of the flood due to the climate change. Keywords : frequency analysis, flood discharge, Han river.

• Electrical & Electronic Materials
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• v.10 no.6
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• pp.533-540
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• 1997

The microwave dielectric properties of ((P $b_{1-x}$ C $a_{x}$)Zr $O_3$ and (P $b_{0.63}$,C $a_{0.37-x}$ $M_{x}$)Zr $O_3$(M=Mg,Sr) ceramics were investigated. In (P $b_{1-x}$ C $a_{x}$)Zr $O_3$ (X=0.33~0.40) ceramics, high quality factor and small temperature coefficient of resonant frequency were obtain in (P $b_{0.63}$C $a_{0.37}$)Zr $O_3$with perovskite structure. In the case of (P $b_{0.63}$C $a_{0.37-x}$M $g_{x}$)Zr $O_3$ dielectric constant temperature coefficient of resonant frequency increased and quality factor decreased due to increase of polarization of A-O bonding. When replacing Ca ion with Sr ion with large ion radius, polarization decreased with increased of bonding length and thus dielectric constant and temperature coefficient of resonant frequency decreased.decreased.creased.

• Korean Journal of Microbiology
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• v.27 no.4
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• pp.334-341
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• 1989

Toluate degradative plasmid from Pseudomonas cepacia SUB37 was determined to be molecular weight as $79.3\times 10^6$

• Journal of the Speleological Society of Korea
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• v.36 no.37
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• pp.33-37
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• 1994

한반도는 지질적으로 안정된 지괴를 유지하고 있기 때문에 활화산이 없고 화산지형도 극히 제한된 지역에 분포하고 있다. 지질사적으로 중생대 이전의 화산활동을 제외하면, 주로 현지 표면상에 나타나는 화산활동의 흔적에 의한 지형들은 대게 신생대 제3기의 주요 화산활동으로써 백두산(2744m), 무두봉(1930m), 대연시봉(2360m), 북포태산(2289m), 남포태산(2435m), 소연지봉(2123m), 관모봉(1387m), 소배산(2174m), 두류산(2309m), 칠보산(906m) 등이 백두산 화산대(그림 1)의 열하를 따라 남동쪽 설령(2442m), 만탑산(2205m) 과 거의 일직선상의 수많은 분출공에서 유동성이 큰 현무암의 분출이 있었다.(중략)

• Journal of Microbiology and Biotechnology
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• v.32 no.5
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• pp.672-679
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• 2022

Microbial lipases are used widely in the synthesis of various compounds due to their substrate specificity and position specificity. 4-Ethyl malate (4-EM) made from diethyl malate (DEM) is an important starting material used to make argon fluoride (ArF) photoresist. We tested several microbial lipases and found that Photobacterium lipolyticum M37 lipase position-specifically hydrolyzed DEM to produce 4-EM. We purified the reaction product through silica gel chromatography and confirmed that it was 4-EM through nuclear magnetic resonance analysis. To mass-produce 4-EM, DEM hydrolysis reaction was performed using an enzyme reactor system that could automatically control the temperature and pH. Effects of temperature and pH on the reaction process were investigated. As a result, 50℃ and pH 4.0 were confirmed as optimal reaction conditions, meaning that M37 was specifically an acid lipase. When the substrate concentration was increased to 6% corresponding to 0.32 M, the reaction yield reached almost 100%. When the substrate concentration was further increased to 12%, the reaction yield was 81%. This enzyme reactor system and position-specific M37 lipase can be used to mass-produce 4-EM, which is required to synthesize ArF photoresist.

• Kyungpook Mathematical Journal
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• v.53 no.1
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• pp.37-47
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• 2013

Let M and X be right R-modules. We introduce several modules relative to the class of B(M, X) and we investigate relation among these modules. In this note, we show if M is X-${\oplus}$-supplemented such that $M=M_1{\oplus}M_2$ implies $M_1$ and $M_2$ are relatively B-projective, then M is an X-H-supplemented module.