• 한국양식학회지
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• 제21권1호
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• pp.19-25
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• 2008

본 연구는 어린 돌기해삼 Stichopus japonicus 사료의 적정 단백질과 지질 함량을 조사하기 위해 단백질(10%, 20%, 30% 및 40%)과 지질(3%, 7% 및 11%)이 달리 함유된 12 종류의 실험사료를 제조하였다. 평균 체중 1.1 g의 치삼을 각 수조마다 50마리씩 3반복으로 수용하여 8주간 수행하였다. 사육실험 종료 후, 생존율은 86% 이상으로 실험구간에 유의한 차이가 없었다(P>0.05). 증중율과 일간성장율은 동일한 지질함량에서 단백질 30%에 지질 3% 함유 사료 공급구가 다른 실험구에 비해 유의하게 높은 값을 나타내었다(P<0.05). 치삼의 수분, 지질 및 회분 함량은 사료의 단백질과 지질함량에 따른 차이를 보이지 않았으나, 단백질함량은 실험구간에 유의한 차이를 보였으며(P<0.05), 단백질 30%에 지질 3% 함유 사료 공급구에서 가장 높게 나타났고, 단백질 20%에 지질 11% 공급구에서 가장 낮았다. 지방산 조성은 사료의 지방산에 영향을 받아 지질함량이 증가함에 따라 18:2n-6은 증가하였고 n-3HUFA는 감소하는 경향을 보였다. 이상의 결과로부터, 해삼의 최대 성장에 필요한 단백질함량은 30%이고, 지질함량은 3%로 판단된다.

• Fisheries and Aquatic Sciences
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• 제21권10호
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• pp.30.1-30.6
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• 2018

A study of three feeding trials was conducted to investigate the dietary protein requirements of Pacific white shrimp (Litopenaeus vannamei) at three different growth stages. Six experimental diets were formulated to include increasing protein levels of 25, 30, 35, 40, 45, and 50% (designated as P25, P30, P35, P40, P45, and P50, respectively) for three feeding trials. The three feeding trials were conducted in different-sized shrimp at 0.65 g (trial 1), 4.80 g (trial 2), and 10.5 g (trial 3). Triplicate groups of shrimp were fed one of the experimental diets for 36, 42, and 48 days in trials 1, 2, and 3, respectively. In trial 1, the growth performance was not affected by the dietary protein levels. However, protein efficiency ratio was significantly higher in P30 diet compared to P40, P45, and P50 diets. In trial 2, growth rate was significantly higher in P35 diet than in P25 diet. In trial 3, the lowest growth performance was obtained in P25 diet which significantly differed from that of other experimental diets. Broken line analysis of growth data indicates that the optimal dietary level of crude protein is 34.5, 35.6, and 32.2% for small-, medium-, and large-sized (juvenile, sub-adult, and adult stages) Pacific white shrimp, respectively.

• KSBB Journal
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• 제30권6호
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• pp.296-301
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• 2015

Eggshell-based biocomposites have become attractive due to their exquisite nanostructure and biological properties, which are mainly composed of highly organized calcium carbonate crystals controlled by organic macromolecules such as proteins and polysaccharides. Here, we designed the recombinant fusion protein of a putative eggshell matrix protein named as GG1234 and a fluorescent reporter protein of DsRed. The protein was successfully over-expressed in E. coli and purified by Ni-NTA affinity chromatography. In vitro calcium carbonate crystallization was conducted in the presence of the fusion protein, and morphological change was investigated. The protein inhibited the calcite growth in vitro, and spherical calcium carbonate micro-particles with the diameter of about $20-30{\mu}m$ were obtained. We expect that this study would be helpful for better understanding of eggshell-based biomineralization.

• Applied Biological Chemistry
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• 제36권3호
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• pp.172-177
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• 1993

참깨박에 함유되어 있는 불용성 형태의 단백질을 Bacillus sp. CW-1121이 생성하는 효소를 이용하여 가용성 형태의 단백질로 용출시키기 위하여 효소를 작용시켰다. 효소 처리 참깨박 단백질의 기포 형성력은 염용성 단백질이 수용성 단백질에 비해 현저히 낮았다. 기포 안정성은 염용성 단백질이 10분 이내 크게 감소된 반면 수용성 단백질의 기포 안정성은 30분 까지 완만하게 감소하였다. 유화력은 각 단백질의 등전점 부근에서 가장 낮았으며, 염용성 단백질이 수용성 단백질에 비해 낮았다 유화 안정성은 유화력과 비슷한 크기로 나타나 $80^{\circ}C$에서의 30분 가열에서도 이들 단백질이 열에 안정함을 보였다. 유지 및 수분 흡착력은 염용성 단백질이 수용성 단백질 보다 높은 값을 나타내었다.

• Preventive Nutrition and Food Science
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• 제19권3호
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• pp.156-163
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• 2014

We investigated the effects of a high-protein diet and resveratrol supplementation on immune cells changes induced by abdominal irradiation in rats. Female Wistar rats were divided into 5 groups: 1) control diet, 2) control diet with irradiation 3) 30% high-protein diet with irradiation, 4) normal diet with resveratrol supplementation and irradiation, and 5) 30% high-protein diet with resveratrol supplementation and irradiation. We measured blood protein and albumin concentrations, lipid profiles, white blood cell (WBC) counts, proinflammatory cytokine production, and splenocyte proliferation in rats that had been treated with a 17.5 Gy dose of radiation 30 days prior. A high-protein diet affected plasma total cholesterol and very low density lipoprotein-cholesterol levels, which were increased by the radiation treatment. In addition, the lymphocyte percentage and immunoglobulin M (IgM) concentration were increased, and the neutrophil percentage was decreased in rats fed a high-protein diet. Resveratrol supplementation decreased the triglyceride (TG) level, but increased the IgM concentration and splenocyte proliferation. Proinflammatory cytokine production was lower in rats fed a high-protein diet supplemented with resveratrol than in rats fed a control diet. The results of the present study indicate that high-protein diets, with or without resveratrol supplementation, might assist with recovery from radiation-induced inflammation by modulating immune cell percentages and cytokine production.

• Journal of Applied Biological Chemistry
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• 제42권4호
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• pp.161-165
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• 1999

The antiviral activity of CAP30 from Chenopodium album, a type1 ribosome-inactivating protein (RIP), was examined against 5 different plant viral pathogens, and its activity against Tobacco mosaic virus was compared to those of well known antiviral proteins such as Pokeweed Antiviral protein from leaves and seeds. When the inoculating concentration of Tobacco mosaic virus was varied from 0.4 to $400{\mu}g/ml$, it was observed that CAP30 at the concentration of $1{\mu}g/ml$ suppressed the viral infection of C. amaranthicolor and C. quinoa almost completely up to $40{\mu}g/ml$ Tobacco mosaic virus. Results from the assays for the inhibitions of in vitro translation of rabbit reticulocyte lysate and the suppression of Tobacco mosaic virus infection ($10{\mu}g/ml$) to C. quinoa indicated that CAP30 is a strong inhibitor of protein synthesis and virus infection. The infection of several viruses other than Tobacco mosaic virus to host plants were also inhibited by $5{\mu}g/ml$ CAP30, suggesting that a gene encoding CAP30 can be used to develop transgenic virus-resistant plants.

• Fisheries and Aquatic Sciences
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• 제18권3호
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• pp.265-271
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• 2015

A feeding trial of four dietary protein levels (20, 30, 40, and 50%) and two lipid levels (7 and 14%) with a factorial design was conducted to determine the optimal dietary protein and lipid levels for juvenile Israeli carp Cyprinus carpio. Triplicate groups of fish (average body weight, $1.3{\pm}0.02g$) were fed the experimental diets for 9 weeks. Survival of fish was not affected by either dietary protein or dietary lipid level. Weight gain and feed efficiency increased as dietary protein levels increased up to 40 and 50%, respectively. Weight gain was higher in fish fed the high-lipid diets with 20 and 40% protein content. Feeding efficiency increased as the dietary lipid level increased for the 30, 40, and 50% protein diets. Daily feed intake decreased with increasing protein level and the minimum feed consumption was observed in fish fed the 50% protein diet with 14% lipid content. Moisture and lipid contents of the whole body were affected by both dietary protein and lipid levels. The crude lipid content of fish fed the 14% lipid diet was higher than that of fish fed the 7% lipid diet at each protein level. The results of this study indicate that a diet containing 40% protein with 14% lipid content is optimal for the growth and effective protein utilization of juvenile Israeli carp.

• 한국산학기술학회논문지
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• 제12권4호
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• pp.1763-1769
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• 2011

한국산 청삼종자를 식품산업 분야에서 응용하기 위해서 청삼종자의 영양화학적 분석을 실시하였다. 청삼종자는 약 30.52%의 지방과 30.02%의 단백질로 구성되어 있었다. 청삼종자 지방은 약 88%의 불포화지방산으로 구성되어 있었으며, 오메가-6와 오메가-3 지방산 함량의 비율이 약 3:1이었다. 또한 청삼종자는 높은 필수 아미노산 함량을 나타내었다. 뿐만 아니라 산-염기 침전법으로 추출한 청삼종자 단백질은 자유라디칼 소거활성을 나타내었다. 이러한 결과들은 청삼 헴프단백질이 우수한 단백질 공급원으로 사용될 수 있음을 보여준다.

• Bulletin of the Korean Chemical Society
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• 제30권8호
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• pp.1891-1892
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• 2009

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2001년도 학술 발표회 진행표 및 논문초록
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• pp.46-46
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• 2001

Evidence has suggested that an anion channel blocker, 4, 4'-diisothiocyanatostilbene-2, 2' disulfonic acid (DIDS) could trigger Ca release from skeletal sarcoplasmic reticulum (SR) by binding to a 30 kDa SR protein. Since the high molecular weight $Ca^{2＋}$ release channel (CRC)/ryanodine receptor (RyR) is the main SR protein that conducts $Ca^{2＋}$ efflux in skeletal muscles, the relationship between CRC and the 30kDa protein remains to be elucidated.(omitted)