• IMMUNE NETWORK
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• 제1권3호
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• pp.250-259
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• 2001

To determine if initial infection with Mycobacterium tuberculosis changes the balance of cytokines between T cells and macrophages, we evaluated interferon (IFN)-${\gamma}$), interleukin-12 (IL)-12, and tumor necrosis factor (TNF)-${\alpha}$ productions by peripheral blood mononuclear cells (PBMC) from 15 untreated active pulmonary tuberculosis (TB) patients and 12 healthy tuberculin reactors (HTR). Freshly isolated PBMC were stimulated with Triton X-100 solubilized protein (TSP), 30-kDa or purified protein derivatives (PPD) antigen for 6, 18 and 96 hours. IL-12 p40 production by antigen-stimulated PBMC from TB patients was significantly decreased compared with that in HTR. In addition, IFN-${\gamma}$ production was significantly depressed in TB patients than that in HTR at a 96-hr stimulation. However, TNF-${\alpha}$ production was significantly higher in antigen-stimulated PBMC from TB than that of HTR. A pronounced increase in IFN-${\gamma}$ protein followed neutralization of IL-10 in early TB patients. However, neutralization of TNF-${\alpha}$ did not significantly alter IFN-${\gamma}$ induction in PBMC from TB patients. There were no significantly differences in the cytokine productions among three proteins, TSP, 30-kDa or PPD antigen. These results indicate that development of TB may be strongly associated with dysregulated productions of IL-12, IFN-${\gamma}$ and TNF-${\alpha}$, during the initial immune responses to M. tuberculosis. Further understanding of operative cytokine networks during human immune cell responses to protein antigens of M. tuberculosis may improve strategies for vaccine development.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.III
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• pp.792-802
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• 2000

The coagulation of Chinese cabbage juice can be accomplished by applying the combine method of the formic acid with rate of 3% and in four hours the propionic acid with rate of 1 % in the juice. The separation of coagulation into the protein paste and the brown juice completed in 6.5 hours by set up method in special storage. The protein paste can be stored safely for 30 days in anaerobic condition.

• Journal of Periodontal and Implant Science
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• 제36권1호
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• pp.155-165
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• 2006

The results of this study confirm that the availability of hemin influences the expression of selected membrane proteins of Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. A 30 kDa (heated 24 kDa) hemin-binding protein whose expression is hemin regulated was identified and purified in P. gingivalis. A strong hemin-binding function was found by LDS-PAGE and TMBZ staining when P. gingivalis cells were grown under hemin-limited conditions. A 50 kDa cell envelope associated protein, whose expression is hemin regulated, is considered to be a putative hemin binding protein from P. intermedia and P. nigrescens, respectively. N-terminal amino acid sequence analysis of CNBr-digested 24 kDa hemin binding protein from P. gingivalis revealed that this protein belongs to a new, so far undescribed hemin-binding class of proteins. N-terminal amino acid sequence of a 50 kDa putative hemin binding protein from P. intermedia was identical with Enolase from Streptococcus intermedia. Work is in progress to further characterize the molecular structure of these proteins.

• Parasites, Hosts and Diseases
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• 제40권2호
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• pp.83-88
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• 2002

The 8 kDa antigenic protein of Clonorchis sinensis was partially purified by ammonium sulfate precipitation and subsequently by a column chromatographic steps. The purified protein was separated into 7 and 8 kDa protein bands through SDS-tricine gel electrophoresis, while the protein was fecund to migrate to a 8 kDa band in 7.5-15% SDS-PAGE. The molecular weight of the antigen was estimated to be 110 kDa by Superose 6 HR 10/30 gel filtration. The purified antigen strongly reacted with the human sera of clonorchiasis. The hyperimmune sera of BALB/c mice immunized against the 8 kDa protein were reacted with both the crude extract and the excretory-secretory product of adult worms, but not with the metacercarial extract. Immunohistochemical staining demonstrated that the protein was distributed to the tegument and subtegumental cells and also to the seminal receptacle. The present findings suggest that the 8 kDa protein is a partition of the multicomplek protein originating from various organs of adult C. sinenis, and that it is composed of several 7 and 8 kDa proteins.

• 한국식품영양학회지
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• 제30권2호
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• pp.371-377
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• 2017

Muscle atrophy is characterized by a decrease in the mass of the muscle. With an increase in life expectancy and chronic illnesses, the incidence of muscle atrophy is increasing and the quality of life of patients is decreasing. Thus, reducing muscle atrophy is of high clinical and socio-economic importance. Mistletoe is a semi-parasitic plant that has been used as a traditional medicine in many countries to treat various human illnesses. It has been reported that Korean mistletoe extract (KME) has diverse biological functions including anti-tumor, anti-oxidant, anti-diabetic, anti-obesity properties, and extension of lifespan. Especially, we have recently reported that KME improves exercise endurance in mice, indicating its beneficial roles in enhancing the capacity of skeletal muscle. In this study, we investigated whether KME could activate the signaling pathway related to protein synthesis in a mouse model of muscle atrophy. Interestingly, KME efficiently activated the Akt/mTOR pathway, and Akt and mTOR are important signaling hub molecules for the acceleration of protein synthesis in muscle cells. In addition, KME also increased the activity of S6 kinase which is involved in the regulation of muscle cell size. Moreover, the ERK activity, required for transcription of ribosomal RNA for protein synthesis, was also enhanced in KME-treated mouse muscle. These data support the idea that KME increases muscle mass via increased protein synthesis. Our findings also suggest that Korean mistletoe might be a promising candidate for the development of functional foods that are beneficial for preventing muscle atrophy.

• 한국수산과학회지
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• 제29권4호
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• pp.464-473
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• 1996

쥐노래미의 단백질 요구량을 조사하기 위해 카제인과 북양어분을 단백질원으로 하여, 사료의 단백질 함량이 30, 40, 50, $60\%$가 되도록 조정한 4종의 실험사료와 사료의 적정 에너지 함량을 조사하기 위해 가용에너지/단백질 (EP) 비가 9, 10, 11, 12인 4종의 실험 사료를 각각 설계하여 사육실험을 실시하였다. 29g 전후의 쥐노래미를 2개월간 사료의 단백질 함량별로 2반복으로 사육 실험한 결과, 증체율 및 사료효율은 모두 단백질 $50\%$ 사료에서 가장 좋았고, 단백질 $60\%$ 사료에서 성장효과는 오히려 감소하여 4개 실험구 중에서 가장 낮은 값을 나타내었다 (P<0.05). 수학적인 방법으로 이차회귀곡선상에서 증체율을 지표로 하여 단백질요구량을 추정한 결과 최대 단백질 요구량이 $45\%$로 나타났다. 일일사료섭취율은 실험구간에 서로 차이가 없었으나, 단백질섭취율은 사료의 단백질 함량이 증가됨에 따라 증가하는 경향을 보였으며, 단백질효율은 사료 단백질 함량이 증가될수록 유의하게 감소하였다(P<0.05). 가장 성장이 좋았던 실험구의 일일단백질섭취율이 어체중 100g 당 1.7g 이였으며, 이차회귀곡선상에서는 일일단백질 요구량이 어체중 100g당 1.5g으로 나타났다. 전어체의 수분함량은 사료 단백질 함량 $40\~60\%$구간에서는 차이가 없었으나, 사료 단백질 $30\%$구는 $40\%$구보다 유의하게 낮은 값을 보였다(P<0.05). 단백질과 회분 함량은 실험구간에 차이가 없었으며, 지질 함량은 단백질 $30\%$ 실험구가 가장 높았고, $40\%$ 실험구가 가장 낮았다(P<0.05) E/P 비가 다른 사료로 사육 실험한 결과, 증체율, 사료효율 및 단백질효율이 E/P 비가 12인 실험구가 다른 실험구보다 높은 값을 보였다. 전어체의 수분, 단백질 및 회분 함량은 사료의 에너지 함량에 영향을 받지 않았으나, 지질 함량은 에너지가 높은 사료인 E/P 비 11과 12 사료에서 유의적으로 높은 값을 보였다. 본 실험 결과로부터 평균체중 100g 이하의 성장기 쥐노래미의 단백질 요구량은 $45\~50\%$, 일일단백질 요구량은 어체중 100g 당 $1.5\~1.7g$으로 추정되며, 쥐노래미 사료의 적정 E/P 비는 12 전후가 적당할 것으로 생각된다.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.56-57
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• 2005

• 한국식품영양학회지
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• 제30권4호
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• pp.796-803
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• 2017

This study investigated the crude protein and amino acid contents of local agricultural products widely and specifically grown in Korea, including 25 vegetables and 13 fruits. The crude protein content of vegetables and fruits ranged from 0.46 to 6.53% and 0.29 to 2.23%, respectively. Totally, 17 types of amino acids were found in most samples. The total amino acid content of vegetables and fruits ranged from 457.38 to 9,303.18 mg% and 368.82 to 3,118.75 mg%, respectively. The total amino acid contents of garlic and passion fruit was higher compared to other vagetables and fruits. The calibration curves of the standard components showed good linearity ($r^2$>0.99), except Met ($r^2=0.989$). The limits of LOD and LOQ were in the range 0.034 to $0.991{\mu}g/mL$ and 0.009 to $0.474{\mu}g/mL$, respectively. The results of the study can serve as a fundamental source of information regarding crude protein and amino acids contents in food, for diet planning.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.355-361
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• 2001

A Chromate tolerant strain of Pseudomonas aeruginosa isolated from the effluent of a tannery showed significant enzymatic activity of chromate reduction. Cells grown in chromate-supplemented medium reduced 8 $\mu\textrm{g}$ chromate/mg protein/h in the presence of NADH/NADPH. The chromate reducing activity was inducible as cells pregrown in chromate showed higher chromate reduction. In contrast, the periplasmic fraction of cells gown in chromate reduce $75\%$ chromate in 4 h and the spheroplast fraction failed to do so, indicating that chromate reductase may be located in the periplasm. The presence of a 30 kDa protein in the periplasmic extracts of cells grown in the presence of chromate, but its absence of the protein in cells grown without chromate, points out a possible role of this protein in chromate reduction.

• BMB Reports
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• 제40권1호
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• pp.65-71
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• 2007

The Helicoverpa armigera nucleopolyhedrovirus (HearNPV) ORF80 (ha80) has 765 bp encoding a protein with approximately 254 amino acids and a predicted molecular weight of 30.8 kDa. Homologues of ha80 are found in most baculovirus sequences, including those from lepidopteran NPVs, lepidopteran granuloviruses (GVs), hymenopteran baculoviruses, and one dipteran baculovirus, yet their functions remain unclear. In this study we characterized ha80, and showed that it was transcribed late in infected host cells (HzAM1). The product of ha80 was a 31 kDa protein that was not a structural protein of budded virus (BV) or occlusion-derived virus (ODV) particles. Ha80 was first detected in the cytoplasm of infected HzAM1 cells at 12 h p.i., and was observed in the nucleus at later stages of infection, suggesting that it may be involved in transporting viral proteins into the host cell nucleus or play its roles in the nucleus.