• Korean Journal of Microbiology
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• v.34 no.4
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• pp.212-218
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• 1998

An obligately methylotrophic bacterium which produces extracellular polysaccharide (EPS) was isolated through methanol-enrichment culture technique. The isolate was aerobic, nonmotile, and gram negative rod and exibited catalase, but no oxidase, activity. Plasmid, carotenoid, and poly-${\beta}$-hydroxybutyric acid were not found. The guanine plus cytosine content of DNA was 52-56%. The isolate was found to grow only on methanol and monomethylamine. Growth was optimal ($t_d=2.4h$) at $35^{\circ}C$ and pH 6.5 in a mineral medium containing 0.5% (v/v) methanol, 25 mM phosphate, and 0.212% ammonium sulfate. Methanol was assimilated through the ribulose monophosphate pathway. Maximun amount of EPS was produced in cells growing at the mid-stationary growth phase at $30^{\circ}C$ in a mineral medium (PH 6.5) containing 1.0% (v/v) methanol in the CIN ratio of 54.7. Thin-layer chromatographic and high performance liquid chromatographic analysis revealed that the EPS was composed of glucose and galactose. EPS which was not treated with ethanol (Pbe) exhibited stable viscosity under various concentrations of salts and temperatures hut showed high viscosity at low pH. EPS precipitated with ethanol (Pae) was found to be more stable in viscosity than the Pbe at various salt concentrations, temperatures, and pH. The Pae also exhibited higher viscosity than the Pbe and xanthan gum. Scanning electron microscopy revealed that the lyophilized Pbe and Pae have a multi-layered structure and a structure of thick fibers, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.4
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• pp.404-412
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• 1994

Radium isotopes were measured together with oceanographical parameters in the southern coastal region of the Korean East Sea during the period of September $2{\sim}8$, 1991. In September, there were various water masses vertically distributed in this region due to formation of strongly seasonal thermoclines. These water masses were characterized by activity of radium isotopes as well as water temperature and dissolved oxygen. Among the water masses, Japan Sea Proper Water(JSPW) below $1^{\circ}C$ had the highest Ra-226 activities but the lowest Ra-228 concentrations. However, Tsushima Surface Water (TSW) above $20^{\circ}C$ in water temperature had the highest Ra-228 which decreased sharply with depth. In TSW, Ra-228 activities were in the range of $194{\sim}270$ dpm/kl, which were approximately 10 times higher than JSPW. Activity ratios(A.R's) of Ra-228/Ra-226 were $1.9{\sim}2.6$ for TSW, $0.7{\sim}1.1$ for Tsushima Middle Water(TMW) of $12{\sim}17^{\circ}C\;to\;0.4{\sim}0.7$ for North Korea Cold Water(NKCW) with $1{\sim}7^{\circ}C$ and below 0.2 for JSPW. The Ra-228/Ra-226 ranged from 0.6 to 0.9 in the cold waters of $2{\sim}6^{\circ}C$, which were observed at depth of $65{\sim}120\;m$ in this study area. Radium isotopes provided a useful means of identifying origins of the cold water which occurred annually at intermediate or bottom layers in the southern coastal zone of the Korean East Sea. By plotting radium isotopes against water temperature, it could be observed clearly that the cold waters between $2{\sim}6^{\circ}C$ did not originate from the mixed water of JSPW and TMW but from NKCW.

• KSBB Journal
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• v.15 no.2
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• pp.125-133
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• 2000

Specific carotenoids and astaxanthin biosynthesis power of Phaffia rhodozyma mutant 876, which was obtained after NTG a and UV treatments, was higher than those of the wild type by 40% and 50%, respectively. The mutant strain did not show t the catabolite repression even at 22% (w/v) glucose concentration. The optimum C{N ratio was 2.0, and the optimum t temperature and initial pH were $22^{\circ}C$ and 6.0, respectively. 80th cell growth and astaxanthin formation decreased drastically a as the fermentation temperature was increased over $22^{\circ}C$, whereas they were comparable in the pH range between 5.0 and 7 7.0. Inoculum size did not affect the final cell density nor the carotenoids biosynthesis, and 3%(v/v) was selected as optimal. H Higher dissolved oxygen concentration facilitated astaxanthin biosynthesis, and aeration rate of 1.0 v/0/m and agitation speed of 400 rpm were selected as optimum. The final cell dens때 of 43.3 g/L and the volumetric astaxanthin and carotenoids concentrations of 110.6 mg/L and 149.4 mg/L, respectively, were obtained. The specific carotenoids concentration was 3.45 m mg{g-yeast(dry). Yx/s and Yp/s values of 0.37 and 1.08 were obtained. The result of this study will provide basic information u useful for mass production of astaxanthin from P. rhodozyma fermentation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.1
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• pp.88-97
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• 1999

The distributional characteristics of chlorophyll-a and their relationship to the oceanographic condition were investigated in the northern part of East China Sea in February, May, August and November during 1995$\~$1997. The temperature and salinity were high in the eastern area and low in the western area. Thermocline and halocline were strongly formed at the 50 m depth in August. Dissolved oxygen was low in August with value of 3.77 ml/$\ell$, particularly in the near coast of East China, where dissolved oxygen was less than 2.0 ml/$\ell$. Transparency was high in May while low in February. Total nitrogen and phosphate concentrations were high in November while silicate concentrations was high in August. They showed the seasonal variations. Total nitrogen and phosphate revealed high concentrations in the near coast of East China, where the salinity was the lowest. Especially, total nitrogen and silicate were higher in August than the other survey months in the near coast of East China. Phosphate showed the even distributional pattern. Chlorophyll-a appeared high in August with 0.512$\mu$g/$\ell$ and low in February with 0.372$\mu$g/$\ell$. Annual means in each survey depth was high at the 10m depth with 0.632 $\mu$g/$\ell$ and gradually decreased toward the more deep depth. But the cencentrations at 150m depth near the bottom again increased as 0.243 $\mu$g/$\ell$. In the seasonal patterns of vortical distribution of chlorophyll-a, the maximum depth value was surface in February, 30 m in May, 10 m in August and 20 m in November. Transparency showed the highest value in May. It means that there are the close relationship between the vertical distribution of chlorophyll-a and transparency.

• Polymer(Korea)
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• v.30 no.4
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• pp.298-304
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• 2006

Blends of poly (acrylic acid-co-maleic acid)(PAM) with poly (vinyl alcohol)(PVA) were obtained by solution blending. The blends were solvent-on to a film to examine thermo-mechanical properties and gas permeability. The transition temperatures $(T_g\;and\;T_m)$ of the blends remained constant regardless of PAM contents. However, the values of enthalpy changes corresponding to melting transition $({\Delta}H_m)$ and initial degradation temperature $({T_D}^i)$ were decreased with increasing PAM content. The values of ultimate strength and initial modulus gave the maximum value at the 12 wt% PAM then decreased with further increase of PAM content up to 15 wt%. To measure the gas permeability of the PVA/PAM blend films, the PVA blend solutions were coated onto both biaxially oriented propylene (BOPP) and poly (ethylene terephthalate)(PET) films. The oxygen transmission rate $(O_2\;TR)$ permeability values mono- tonically decreased with increasing PAM content. However, moisture vapor transmission rate was not affected by PAM content.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2018.06a
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• pp.39-39
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• 2018

최근 플라즈마 의학이 발달하면서 제트, 펜, 니들, 토치 등의 다양한 형태의 플라즈마 발생기가 개발되었으며 내부의 가스라인으로 가스의 종류, 유속, 조성 등을 조절하여 생물학적 효과를 극대화 할 수 있고 안정적으로 플라즈마 방전상태를 유지할 수 있으나 처리 면적이 좁아 실제 생물학적 시스템 (세포, 조직, 그리고 박테리아) 적용에 있어 한계점이 존재한다. 이러한 한계점을 극복하기 위해서 유전체격벽방전 (Dielectric barrier discharge, DBD) 방식을 이용한 플렉서블 활성종 발생기를 제작하고 생물학적 시스템에 적용하기 위한 방전 특성 평가를 진행하였으며, 간단한 in vitro 모델인 한천 젤을 이용하여 플라즈마 처리에 따른 전달물질의 침투거리를 확인하였다. 플라즈마 방전 시 생성되는 수산화기 [OH], 과산화수소 [$H_2O_2$], 초산소음이온 [$O_2{^-}$], 오존 [$O_3$], 그리고 산화질소 [$NO_x$]와 같은 산소 및 질소 활성종 (Reactive oxygen and nitrogen species, RONS)은 세포벽 또는 세포막의 주요 구성성분인 다당류와 인지질의 과산화 반응을 통해 구조를 변화시키고 생물학적 시스템의 표면의 pH를 낮춘다. 이러한 RONS의 작용은 살균, 소독 뿐만 아니라 약물의 침투를 돕는다. 일반적으로 한천 겔은 농도에 따라 생체 내 뇌 조직과 물리적 특성이 유사하고, 미생물학 기질, 방사선학 연구를 위한 조직모델로 사용되기 때문에 본 연구에서는 3%와 5% 농도의 한천 젤을 사용하여 침투거리를 확인하였다. 한천 젤은 $2.5{\times}2.5{\times}2.5cm^3$의 크기로 준비되었고 대조군으로 염료가 포함된 에멀젼을 0.01 g 도포하고, 실온에서 30분간 보존 후 단면을 잘라 현미경으로 침투거리를 확인하였으며, 실험군으로 플라즈마 전처리 후 에멀젼을 도포한 시표와 에멀젼 도포 후 플라즈마 처리한 시료에 대해 에멀젼 침투거리의 변화를 확인하였다. 본 연구의 플렉서블 활성종 발생기는 인체에 부착하여 사용되기 때문에 화상, 홍반을 유발을 방지하기위해 $40^{\circ}C$의 온도에서 실험을 진행하였고 이때에 플라즈마 방전조건은 $0.065W/cm^2$ 수준의 전력을 소모하는 1.7 kV의 전압, 16 kHz의 주파수로 10분간 처리하였다. 그 결과 3%의 한천 젤의 경우 침투거리 0.779 mm에서 0.826 mm, 0.942 mm까지 침투거리가 증가하였고 5%의 한천 젤의 경우 0.859 mm, 0.949 mm로 증가하였다. 이러한 침투거리 증가는 젤 표면의 다당류를 구성하고 있는 단량체가 플라즈마 처리시 화확적 구조가 끊어져 결론적으로 약물 침투가 증가된 것으로 판단된다.

• Korean Journal of Food Science and Technology
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• v.46 no.5
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• pp.622-629
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• 2014

We studied the effects of Akebia quinata fruit extract (AQ) on acute alcohol-induced hepatotoxicity in mice. AQ (30-1,000 mg/kg body weight (BW) per day) was orally administered to the study group, once daily for 1 week. On the last day of AQ treatment, ethanol (6 mg/kg BW) was orally administered to induce acute liver injury. The AQ-treated group showed significantly lower levels of alanine aminotransferase and aspartate aminotransferase, compared to the only ethanol-treated group (ETG). The glutathione level in the AQ-treated group elevated up to 20.6%, compared to that observed in the ETG. The mRNA expression of glutathione synthetic enzymes was also higher in the AQ-treated group, compared to the ETG. The AQ-treated group also exhibited lower levels of expression of NADPH oxidase 4 and tumor necrosis factor alpha mRNA. Thus, these results show that AQ treatment can be a potential method to reduce oxidative stress and inflammation in ethanol-treated mouse liver and also that AQ can be a useful therapeutic agent for acute alcohol-induced hepatotoxicity.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.442-450
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• 1997

The chemical properties of water masses were investigated at 33 stations of the southeastern last Sea in February, 1995 on board R/V Tam-Yang. The water masses were not clearly distinguished due to the vortical mixing in winter. However, on the basis of the T-S and $T-O_2$ diagrams, water masses in the study area were divided into five groups (Type I, Type II, Type III, Type IV, Type V). (1) $>9.0^{\circ}C,\;>34.35\;psu,\;5.08\~5.60m\ell/\ell$ at Type I, (2) $6.0\~9.0^{\circ}C,\;34.15\~34.35\;psu,\;5.60\~5.90\;m\ell/\ell$ at Type II, (3) $4.0\~6.0^{\circ}C,\;34.00\~34.15\;psu,\;>5.90m\ell/\ell$ at Type III, (4) $1.5\~4.0^{\circ}C,\;34.00\~34.05\;psu,\;5.40\~5.90\;m\ell/\ell$ at Type IV, (5) $<1.5^{\circ}C,\;34.05\~34.07\;psu,\;4.80\~5.40\;m\ell/\ell$ at Type V. In the vertical profiles of nutrients, the concentrations were very low in the surface layer and increased rapidly with depth. The highest concentrations occurred in Type IV, while the concentrations in Type I were the lowest. The N/P ratios were less than Redfield ratio, indicating that nitrogenous nutrients were the limiting factor tor phytoplankton growth. The concentrations of POC and PON were in the range of $0.49\~20.03\;{\mu}g-at/\ell\;and\;0.09\~5.34\;{\mu}g-at/\ell$, respectively. The relatively high concentration occured in the surface layer of inner shore, showing that the concentration at each water mass followed the order Type I > Type II > Type III > Type IV > Type V, respectively. The C:N ratio in particulate organic matter was lower than the values reported in other region due to relatively high concentrations of PON in the study area. Relatively high ratios of POC to chlorophyll $\alpha$ during the study periods indicate that non-living detritus comparised most of the POC in the study area.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.1
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• pp.8-15
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• 2003

If contaminated river water is sprayed over a floodplain, the microbial processes can simultaneously remove organic matter and nitrogen during the infiltration through the sediment profile. The effect of rhizosphere on the removal of organic matter and nitrogen from contaminated river water was investigated using floodplain lysimeters. River water was sprayed at a rate of $68.0L\;m^{-2}\;d^{-1}$ on the top of the lysimeters with or without weed vegetation on the surface, Concentrations of $NO_3$, $NH_4$ and dissolved oxygen (DO), and chemical oxygen demand (COD) and Eh in water were measured as functions of depth for 4 weeks after the system reached a steady state water flow and biological reactions. A significant reductive-condition for denitrification developed in the 30-cm surface profile of lysimeters with weeds. At a depth of 30 cm, COD and $NO_3$-N concentration decreased to 5.2 and $0.9mg\;L^{-1}$ from the respective influent concentrations of 18.2 and $9.8mg\;L^{-1}$. The removal of $NO_3$ in lysimeters with weeds was significantly higher than in those without weeds. Vegetation on the top was assumed to remove $NO_3$ directly by absorption and to create more favorable conditions for denitrification by supply of organic matter and rapid $O_2$ consumption, In the lysimeters without weeds, further removal of $NO_3$ was limited by the lack of an electron donor, i.e. organic matter. These results suggest that the filtration through native floodplains, which include rhizospheres of vegetation on the surface, can be effective for the treatment of contaminated river water.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.8
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• pp.1286-1293
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• 2004

This study was conducted to investigate the biological activity and hepatoprotective effect of various fractions and isolated compounds from Kochiae fructus (KF) extract on D-galactosamine (GaIN)-intoxicated rats. Male Sprague-Dawley rats were divided into control, GaIN treated group (GaIN), GaIN plus KF methanol extract treated group (KFM 200-GaIN), GaIN plus KF butanol extract treated group (KFB 200-GaIN), GaIN plus momordin Ic treated group (Momordin Ic 30-GaIN) and GaIN plus oleanolic acid treated group (Oleanolic acid 30-GaIN). KFM (200 mg/kg BW), KFB (200 mg/kg BW), momordin Ic (30 mg/kg BW) and oleanolic acid (30 mg/kg BW) were orally administered once a day for 14 days. GaIN (400 mg/kg BW) was injected at 30 minutes after the final administration of the compounds. The activities of serum aspartate aminotransferase and alanine aminotransferase were increased in the GaIN group compared to the control group and significantly lower in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Hepatic lipid peroxide level was increased in the GaIN group compared to the control group and was lower in the KFM 200-GaIN, KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Activities of xanthine oxidase and aldehyde oxidase in liver were higher in the GaIN group than in the control group and were significantly decreased in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group compared to the GaIN group. Hepatic glutathione, ${\gamma}$-glutamylcysteine synthetase and catalase activities were decreased in the GaIN group compared to the control group and were higher in the KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group than in the GaIN group. Activities of hepatic glutathione reductase, glutathione S-transferase, superoxide dismutase and glutathione peroxidase were lower in the GaIN group than in the control group and were improved in the KFM 200-GaIN, KFB 200-GaIN, momordin Ic 30-GaIN and oleanolic acid 30-GaIN group compared to the GaIN group. Therefore, the current results indicate that momordin Ic administration alleviated the GaIN-induced adverse effect through enhancing the antioxidant enzyme activities.