• Fisheries and Aquatic Sciences
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• 제22권12호
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• pp.28.1-28.8
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• 2019

Background: In the present study, we evaluated four commonly used housekeeping genes, viz., actin-β, elongation factor-1α (EF1α), acidic ribosomal protein (ARP), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as internal references for quantitative analysis of immune genes in nervous necrosis virus (NNV)-infected seven-band grouper, Hyporthodus septemfasciatus. Methods: Expression profiles of the four genes were estimated in 12 tissues of healthy and infected seven-band grouper. Expression stability of the genes was calculated using the delta Ct method, BestKeeper, NormFinder, and geNorm algorithms. Consensus ranking was performed using RefFinder, and statistical analysis was done using GraphpadPrism 5.0. Results: Tissue-specific variations were observed in the four tested housekeeping genes of healthy and NNV-infected seven-band grouper. Fold change calculation for interferon-1 and Mx expression using the four housekeeping genes as internal references presented varied profiles for each tissue. EF1α and actin-β was the most stable expressed gene in tissues of healthy and NNV-infected seven-band grouper, respectively. Consensus ranking using RefFinder suggested EF1α as the least variable and highly stable gene in the healthy and infected animals. Conclusions: These results suggest that EF1α can be a fairly better internal reference in comparison to other tested genes in this study during the NNV infection process. This forms the pilot study on the validation of reference genes in Hyporthodus septemfasciatus, in the context of NNV infection.

• 대한환경공학회지
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• 제32권6호
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• pp.615-624
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• 2010

낙동강 수계에서의 인공 사향물질들의 검출현황을 조사한 결과, 다환 사향물질인 HHCB, AHTN, AHMI 및 ADBI 4종과 염화 사향물질인 MK 1종이 검출었으며, 본류에서는 HHCB, AHTN 및 MK 3종만이 검출되었다. 낙동강 수계에 검출된 인공 사향물질들의 구성비율을 조사한 결과, HHCB가 50% 이상을 차지하여 가장 높게 나타났고, 다음으로 MK와 AHTN이 높은 비율을 나타내었다. 낙동강 본류에서 가장 높은 검출농도를 나타낸 지점은 고령지점 (고령교)으로 2월과 9월에 각각 280.4 ng/L와 195.3 ng/L가 검출되었고, 지류에서는 진천천 지점으로 2월에 2146.6 ng/L와 9월에 1386.4 ng/L의 검출농도를 나타내었다. 인공 사향물질들은 낙동강 상류부근에서는 거의 검출되지 않았으나 중류부근인 구미 지점부터 하수처리장 방류수의 영향을 받아서 농도가 증가하였고, 금호강과 진천천 (hot spot)의 영향을 많이 받는 고령지점에서 최대농도를 나타낸 후 하류로 갈수록 희석효과에 의해 농도가 점점 감소하였다.

• 한국운동역학회지
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• 제25권3호
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• pp.257-263
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• 2015

Objective : The purpose of this study was to investigate the effect of genu valgum on the body mass index, movement of lower limb joints, and ground reaction force. Methods : Gait patterns of 30 college students with genu valgum were analyzed and the static Q angle of the femur was measured for selecting genu valgum of the subjects. To analyze the kinetic changes during walking, the six-camera Vicon MX motion analysis system was used. The subjects were asked to walk 12 meters using the more comfortable walking method for walking. After they walked 12 meters more than 10 times, their most natural walking patterns were chosen three times and analyzed. Results : As a result of measuring a relationship between genu valgum and Q-angle, as the Q-angle increases, it showed a genu valgum also increased. Body Mass Index showed a significant difference between the groups was higher in the genu valgum group.(p<.001). The analysis result showed that genu valgum had a significant effect on the internal rotation moment in the hip joint(p<.05). Also, genu valgum had a significant effect on the internal rotation moment of the knee joint(p<.05). The comparative analysis of the Medial-Lateral ground reaction force in the genu valgum group showed a tendency to increase the medial ground reaction force(p<.05). The vertical ground reaction forces of the middle of the stance phase(Fz0) showed a significant increase in genu valgum group(p<.05), in particular the results showed a decrease in the early stance phase(p<.001). Conclusion : In conclusion, the change in body mass is considered to be made by proactive regular exercise for improvement of the genu valgum. In addition, the prevention of the deformation caused by secondary of the genu valgum in this study may be used as an indicator of the position alignment rehabilitation for structural and functional improvements. Applying a therapeutic exercise program for the next lap will require changes in posture alignment.

• 한국운동역학회지
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• 제19권3호
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• pp.519-528
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• 2009

상해 예방을 위해 이용하는 테이핑의 효과는 이미 선행연구들에서 이해할만하게 이야기 되어져 왔지만, 기능적 발목 불안정성을 가진 대상자에게 동적 임무를 가지고 지면반력과 안정성을 분석한 연구는 거의 없다. 본 연구는 발목 불안정성을 가진 선수들을 대상으로 점프 후 착지 시 지면반력 변인과 안정성에 미치는 영향을 알아보는데 있다. 이 실험을 위하여 기능적 발목 불안정성을 가진 14명의 선수가 참가하였고 동작분석과 지면반력 값을 산출하기 위해 적외선 카메라 8대(Vicon MX-F20, Oxford Metric Ltd, Oxford, UK)로 구성된 동작분석시스템(Vicon Motion Systems)과 지면반력기를 사용하였다. 본 연구 결과 발목 불안정성이 있는 선수에게 테이핑의 적용은 착지 시 배측굴곡 각속도, 내번 각속도, 최대 수직지면반력을 감소시켰으며, 안정성과 관련된 변인인 A-P cop, M-L cop에서 안정성을 향상시켰다. 임상에서 발목 불안정성이 있는 선수들에게 상해 예방을 위한 하나의 방법으로 테이핑의 사용을 권장해도 될 것으로 사료된다.

• 대한치과교정학회지
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• 제44권4호
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• pp.168-176
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• 2014

Objective: The aim of this randomized controlled clinical trial was to compare oral health-related quality of life (OHRQoL) of patients treated with conventional, active self-ligating (ASL), and passive self-ligating (PSL) brackets in different therapeutic phases. Methods: Sixty patients (mean age 18.3 years; 29 males and 31 females) requiring orthodontic treatment were randomly and equally assigned to receive conventional (Victory Series), ASL (In-Ovation R), or PSL (Damon 3MX) brackets. OHRQoL was measured with a self-administered modified 16-item Malaysian version of the Oral Health Impact Profile for immediate (soon after the visit) and late (just before the subsequent visit) assessments of the bonding and activation phases. Data were analyzed with the Kruskal-Wallis and chi-square tests. Results: The PSL and ASL groups showed more immediate and late impacts in the bonding phase, respectively; the conventional group was affected in both the assessments. The first activation phase had similar impacts in the groups. After the second activation, the conventional group showed more immediate impacts, whereas the PSL and ASL groups had more late impacts. The commonly affected domains were "physical disability," "functional limitation," "physical pain," and "psychological discomfort." No significant differences in the prevalence and severity of immediate and late impacts on OHRQoL of the patients were noted in any therapeutic phase. Conclusions: No bracket system seems to ensure superior OHRQoL. This information could be useful for explaining the therapeutic phases, especially the initial one, and selecting the optimal bracket system based on the patient's preference.

• 한국자기학회지
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• 제25권4호
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• pp.106-110
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• 2015

Fe 원자 2개를 포함한 2종류의 이중 철 효소의 전기구조 및 자기적 성질을 제1원리의 범밀도함수법을 이용하여 계산하였다. 계산 된 결과, 전자구조는 주위 6개의 배위 원자로 인해 비슷한 모습을 보이고 있었다. 총 에너지 계산에서는 두 종류 모두 반강자성적 상호작용이 낮은 에너지를 가지고 있었다. 계산 된 $Fe^{+3}$ 원자 사이의 교환상호작용 크기는 diiron-4가 diiron-2보다 한 단위 큰 값을 얻었고, 실험과 잘 일치하였다. 이 원인은 diiron-4가 더 가까운 Fe-O 원자거리를 가지고 있었고, Fe 사이 산소 매개 원자의 2p 준위가 높아 강한 초교환상호작용을 일으켰다. 또한, 직각에 가까운 diiron-2 Fe-O-Fe 각도보다 커서 Fe-O의 강한 ${\sigma}$ 결합으로 인한 초교환상호작용의 결과이다.

• Restorative Dentistry and Endodontics
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• 제26권5호
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• pp.393-398
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• 2001

Polymerization of light-activated restorations results in temperature increase caused by both the exothermic reaction process and the energy absorbed during irradiation. Within composite resin, temperature increases up to 2$0^{\circ}C$ or more during polymerization. But, insulation of hard tissue of tooth lowers this temperature increase in pulp. However, many clinicians are concerned about intrapulpal temperature injury. The purpose of this study was to evaluate temperature changes in the pulp according to various restorative materials and bases during curing procedure. Caries and restoration-free mandibular molars extracted within three months were prepared Class I cavity of 3$\times$6mm with high speed handpiece fissure bur. 1mm depth of dentin was evaluated with micrometer in mesial and distal pulp horns. Pulp chambers were filled with 37.0$\pm$0.1$^{\circ}C$ water to CEJ. Chromium-alumina thermocouple was placed in pulp horn below restorative materials for evaluating of temperature changes. This thermocouple was connected to temperature-recording device(Multiplication analyzer MX, 6.000, JAPAN). Temperature changes was evaluated from initial 37.$0^{\circ}C$ after temperature changes to 37.$0^{\circ}C$. Tip of curing unit was placed in the center of prepared cavity separated 1mm from restorative materials. Curing time was 40s. The restorative materials were used with Z 100, Fuji II LC, Compoglass flow and bases were used with Vitrebond, Dycal. Resrorative materials were placed in 2mm. The depth of bases were formed in 1mm and in this upper portion, resin of 2mm depth was placed. This procedure was performed 10 times. The results were as follows. 1. All the groups showed that the temperature in pulp increased as curing time increased 2. The temperature increase of glass ionomer was significantly higher than that of Resin and Compomer during curing procedure (P<0.05). 3. The temperature increase in glass ionomer base was significantly higher than that of Calcium hydroxide base during Resin curing procedure (P<0.05).

• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1155-1164
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• 2019

Lichens contain diverse bioactive secondary metabolites with various chemical and biological properties, which have been widely studied. However, details of the inhibitory mechanisms of their secondary metabolites against influenza A virus (IAV) have not been documented. Here, we investigated the antiviral effect of lichen extracts, obtained from South Korea, against IAV in MDCK cells. Of the lichens tested, Nipponoparmelia laevior (LC24) exhibited the most potent inhibitory effect against IAV infection. LC24 extract significantly increased cell viability, and reduced apoptosis in IAV-infected cells. The LC24 extract also markedly reduced (~ 3.2 log-fold) IAV mRNA expression after 48 h of infection. To understand the antiviral mechanism of LC24 against IAV, proteomic (UPLC-$HDMS^E$) analysis was performed to compare proteome modulation in IAV-infected (V) vs. mock (M) and LC24+IAV (LCV) vs. V cells. Based on Ingenuity Pathway Analysis (IPA), LC24 inhibited IAV infection by modulating several antiviral-related genes and proteins (HSPA4, HSPA5, HSPA8, ANXA1, ANXA2, $HIF-1{\alpha}$, AKT1, MX1, HNRNPH1, HNRNPDL, PDIA3, and VCP) via different signaling pathways, including $HIF-1{\alpha}$ signaling, unfolded protein response, and interferon signaling. These molecules were identified as the specific biomarkers for controlling IAV in vitro and further confirmation of their potential against IAV in vivo is required. Our findings provide a platform for further studies on the application of lichen extracts against IAV.

• 대한핵의학회지
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• 제38권4호
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• pp.318-324
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• 2004

목적: Philips GEMINI PET/CT 스캐너는 GSO 섬광결정을 사용해 제작된 전신용 PET/CT 스캐너이다. 이 연구에서는 NEMA에서 새롭게 제안한 NEMA NU2-2001에 따라 GEMINI PET/CT 스캐너의 공간분해능, 민감도, 산란분획, NECR 등을 평가하고 그 결과를 BGO, LSO등의 섬광결정의 특성과 비교하였다. 대상 및 방법: GEMINI는 Philips ALLEGRO PET과 MX8000 D multi-slice CT 스캐너를 결합한 PET/CT 스캐너로서 검출기는 GSO 섬광결정 ($4{\times}6{\times}20mm^3$)을 사용하였고 축방향 시야는 18 cm이다. 공간분해능. 민감도, 산란분획, NECR 등을 평가하기 위하여 PET 데이터를 획득하였다(동시계수창: 8 ns, 에너지창: $409{\sim}664$ keV). 공간분해능 측정을 위하여 축횡단면의 중심에서 1 cm, 10 cm 떨어진 지점의 각 3지점((a) x=0, y=1, (b)x=10, y=0, (c)x=0, y=10)에서 영상을 획득한 다음 여과후역투사방법(램프필터 사용)과 3D RAMLA를 이용하여 영상재구성을 하고 FWHM을 구하였다. 민감도 측정을 위하여 선선원(F-18)을 축횡단면의 중심과 중심에서 10 cm 벗어난 지점에서 5개의 알루미늄관을 차례로 씌워 매질감쇠에 따라 달라지는 참계수를 구하고 이 값을 회귀분석하여 감쇠매질이 없는 이상적인 상황에서의 민감도를 측정하였다(랜덤계수가 참계수의 1%이내). 산란분획과 NECR을 측정하기 위하여 F-18 선선원(1110 MBq)을 산란팬텀에 주입하여 7반감기동안 계수를 획득하였다. SSRB을 사용하여 3D 데이터를 재구성한 다음 랜덤계수율이 참계수율이 1% 미만인 영역에서 산란분획을 구하고 각 횡단면의 값을 평균하여 전체 산란분획을 얻었다. 이 값을 기초로 각 프레임, 각 횡단면에 대한 랜덤계수율, 산란계수율, NECR을 구하였다. 결과: 스캐너의 중심에서 1 cm 벗어난 지점에서 횡축방향, 축방향 공간분해능은 (1) 5.3, 6.5 mm (FBP), (2) 5.1, 5.9 mm (3D RAMLA)이었다. 횡단면의 중심에서 10 cm 벗어난 지점에서 횡축반경방향, 횡축접선방향, 축방향 공간분해능은 (1) 5.7, 5.7, 7.0 mm (FBP), (2) 5.4, 5.4, 6.4 mm (3D RAMLA)이었다. 감쇠매질이 없는 이상적인 상황에서의 민감도는 횡단면의 중심에서 3,620 counts/sec/MBq, 횡단면의 중심에서 10 cm 벗어난 지점에서 4,324 counts/sec/MBq이었다. 산란분획은 40.6%, 최대 참계수율과 최대 NECR은 각각 88.9 kcps @ 12.9 kBq/mL, 34.3 kcps @ 8.84 kBq/mL이었다. 결론: 이 실험에서 NEMA NU2-2001을 이용해 GSO 섬광결정을 사용해 제작된 PET/CT에 대한 성능 평가를 실시하였다. 이는 BGO, LSO 섬광결정을 사용해 제작된 PET 스캐너의 특성과 비교할 수 있는 자료를 제공하며 PET 영상 획득 시 객관적 평가와 분석에 유용하였다.

• Bulletin of the Korean Chemical Society
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• 제32권5호
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• pp.1692-1696
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• 2011

The reactions of 1H-1,2,4-triazole (Htr) with $MX_2$ ($ZnCl_2$ for 1; $CdBr_2$ for 2) resulted in two coordination polymers, [Zn(tr)Cl]$_n$ (1) and $[Cd(Htr)_2Br_2]_n$ (2). The structural analyses indicate that 1 and 2 feature a 2D layer and 1D triple chain, respectively. In 1, neighouring Zn atoms are connected by ${\mu}_3-1$ ${\kappa}N$: 2 ${\kappa}N$: $4{\kappa}N-tr^-$ anionic ligand into 6- and 16-membered rings, further grow into a 2D sheet. Cd atoms in 2 are bonded by two ${\mu}_2-Br^-$ bridges and neutral ${\mu}_2$-1 ${\kappa}N$: 2 ${\kappa}N$-Htr to form a 1D triple chain. The fluorescent characterizations of 1, 2 and the free Htr ligand feature simlilar emission peakes at 444, 446 and 423 nm respectively, which can be assigned to intra-ligand ${\pi}-{\pi}^*$ transition of (H)tr. The energy gaps of 5.90 eV for 1, 5.16 eV for 2, and 5.93 eV for Htr suggest that the compounds behave as insulators.