• Journal of the Optical Society of Korea
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• 제20권1호
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• pp.204-211
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• 2016

Oil pollution seriously endangers the biological environment and human health. Due to the diversity of oils and the complexity of oil composition, it is of great significance to identify the oil contaminants. The 3-D fluorescence spectrum combined with a second order correction algorithm was adopted to measure an oil mixture with overlapped fluorescence spectra. The self-weighted alternating trilinear decomposition (SWATLD) is a kind of second order correction, which has developed rapidly in recent years. Micellar solutions of #0 diesel, #93 gasoline and ordinary kerosene in different concentrations were made up. The 3-D fluorescence spectra of the mixed oil solutions were measured by a FLS920 fluorescence spectrometer. The SWATLD algorithm was applied to decompose the spectrum data. The predict concentration and recovery rate obtained by the experiment show that the SWATLD algorithm has advantages of insensitivity to component number and high resolution for mixed oils.

• 박물관보존과학
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• 제12권
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• pp.9-17
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• 2011

본고는 적색계 염재인 소목, 꼭두서니, 홍화, 자초로 염색한 직물편에 대한 비파괴 자외 가시광 분광분석 및 3차원 형광 분광 분석 내용으로 고대 직물에 염색된 염료 규명을 위한 기초 자료 구축을 위함이다. 직물이나 매염제의 종류에 따라 각 분석 결과에 영향을 주는지 여부를 확인하기 위하여 직물 2 종류(면과 견)와 매염 방법 3 가지(무매염, 백반, 철)로 염색한 직물시편을 제작하였다. 염색 직물편에 대한 자외-가시 분광반사 스펙트럼 결과 소목, 꼭두서니, 자초로 염색한 경우 직물의 종류에 관계없이 매염제에 따른 차이를 보였다. 또한 홍화는 직물 및 매염제에 따른 차이가 없었다. 3차원 형광 스펙트럼 측정결과 소목은 매염제에 따른 차이를 보이며 꼭두서니의 경우 직물에 따른 차이를 보였고, 홍화의 경우 직물과 매염제 관계없이 고유한 형광스펙트럼으로 나타났으며, 자초의 경우 형광스펙트럼이 나타나지 않았다.

• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4247-4252
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• 2011

The porphyrin-incorporated arylether dendrimers ZnP-D1 and ZnP-D4 were investigated to discover the influence of dendritic environments for the axial ligation of pyridine and photoinduced electron transfer by methyl viologen. Absorption and fluorescence spectra of ZnP, ZnP-D1, and ZnP-D4 were measured in dichloromethane with the addition of pyridine or methyl viologen dichloride. Axial ligation of pyridine was confirmed by red-shifted absorption spectrum. The complex formation constants $K_f$ (Table 1) for axial coordination of pyridine on ZnP, ZnP-D1, and ZnP-D4 were estimated to be $4.4{\times}10^3\;M^{-1}$, $3.3{\times}10^3\;M^{-1}$, and $1.7{\times}10^3\;M^{-1}$, respectively. The photoinduced electron transfer to methyl viologen dichloride was confirmed by fluorescence quenching. Stern-Volmer constants Ksv for ZnP, ZnP-D1, and ZnP-D4 were calculated to be $2.6{\times}10^3$, $2.5{\times}10^3$, and $2.1{\times}10^3$, respectively. ZnP-D4 surrounded by 4 aryl ether dendrons shows the smallest $K_f$ and Ksv values, with comparison to ZnP and ZnP-D1.

• 박물관보존과학
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• 제11권
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• pp.61-72
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• 2010

본 고는 우리나라 황색계 염재인 울금, 황벽, 황련, 치자, 괴화로 염색한 직물편에 대한 비파괴 자외-가시광 분광 분석 및 3차원 형광 분광 분석 내용이다. 직물이나 매염제 종류에 따라서 각 염재의 분석결과에 영향을 주는지 여부를 확인하기 위하여 직물 2종류(견과 면)와 매염방법 3가지(무매염, 백반, 철)로 염색한 직물시편을 제작하였다. 이 염색 직물편에 대한 자외-가시 분광반사 스펙트럼 측정 결과, 울금, 황벽, 황련으로 염색한 경우 직물의 종류에 관계없이 무매염과 백반매염한 시편의 결과가 유사한 반면 철매염한 시편에서는 차이를 보였다. 또한 치자로 염색한 경우 직물의 종류에 따라 다른 결과를 보였으나 매염제에는 영향을 받지 않았다. 반면에 괴화로 염색한 경우 직물의 종류에 관계없이 매염제에 따른 차이를 보였다. 3차원 형광 스펙트럼 측정 결과 울금, 황벽, 황련으로 염색한 직물편은 직물과 매염제에 관계없이 고유한 형광 스펙트럼으로 나타났으나 치자의 경우 직물의 종류에 따라 차이를 보인 반면 괴화의 경우는 직물의 종류에는 관계가 없었으나 매염제에 따른 차이를 보였다.

• Journal of Korean Academy of Oral Health
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• 제41권4호
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• pp.290-295
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• 2017

Objectives: Dental plaque emits red fluorescence under a visible blue light near the ultra-violet end of the light spectrum. The fluorescence characteristics of each microorganism have been reported in several studies. The aim of this study was to evaluate changes in red fluorescence of oral microorganisms that is affected by blood in the culture media. Methods: The gram-positive Actinomyces naeslundii (AN, KCTC 5525) and Lactobacillus casei (LC, KCTC 3109) and gram negative Prevotella intermedia (PI, KCTC 3692) that are known to emit red fluorescence were used in this study. Each bacterium was activated in broth and cultivated in different agar media at $37^{\circ}C$ for 7 days. Tryptic soy agar with hemin and vitamin $K_3$ (TSA), TSA with sheep blood (TSAB), basal medium mucin (BMM) medium, and BMM with sheep blood (BMMB) were used in this study. Fluorescence due to bacterial growth was observed under 405-nm wavelength blue light using the quantitative light-induced fluorescence-digital (QLF-D) device. The red, green, and blue fluorescence values of colonies were obtained using image-analysis software and the red to green ratio (R/G value) and red to total RGB ratio (R/RGB value) were calculated for quantitative comparison. Results: The QLF-D images of the AN, LC, and PI colonies showed red fluorescence in all media, but the fluorescence of all bacteria was reduced in TSA and BMM media, compared with in TSAB and BMMB media. Both the R/G and the R/RGB values of all bacteria were significantly reduced in growth media without blood (P<0.001). Conclusions: Based on this in vitro study, it can be concluded that red fluorescence of oral bacteria can be affected by growth components, especially blood. Blood-containing medium could be a significant factor influencing red fluorescence of oral bacteria. It can be further hypothesized that bleeding in the oral cavity can increase the red fluorescence of dental plaque.

• 박물관보존과학
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• 제14권
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• pp.81-89
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• 2013

본고는 우리나라 청색계 염재인 쪽과 황색계 염재인 울금, 치자, 황련, 황벽으로 염색한 청색, 녹색계열의 직물편에 대한 비파괴 자외-가시광 분광 분석 및 3차원 형광 분광 분석 내용이다. 직물의 종류에 따라 각 염재의 분석결과에 영향을 주는지 여부를 확인하기 위하여 견과 면에 염색한 직물시편을 제작하였다. 염색 직물편에 대한 자외-가시광 반사도 측정 결과 쪽으로 염색한 경우 직물의 종류에 관계없이 쪽의 고유한 반사 스펙트럼이 나타났다. 녹색 계열의 직물은 3차원 형광 분광 분석결과 직물의 영향없이 황색 염재인 울금, 황련 및 황벽의 고유한 형광 스펙트럼이 확인되나 치자 및 쪽의 형광 스펙트럼은 나타나지 않았다. 이와 같은 결과는 견이나 면에 염색된 청색 및 녹색 염료의 종류를 규명하는 자료로 활용할 수 있을 것으로 판단된다.

• 한국멀티미디어학회논문지
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• 제22권12호
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• pp.1339-1346
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• 2019

In the characteristic of the brain malignant, the blood vessels and tumors have the same color and shape, and the boundary distinction is not clear, Therefore, it is difficult to observe the naked eye. Because of the high invasiveness, the risk of recurrence is high. Therefore, complete resection of the tumor is essential. The method for distinguishing the boundary between blood vessels and tumors is a fluorescence contrast method using indocyanine green (ICG), a fluorescence contrast agent. In ICG, the concentration range analysis is very important because the fluorescence expression state varies depending on the concentration. However, since the analysis result of the fluorescence expression condition is insufficient according to the current concentration, this paper proposes by analyzing the initial protocol of the concentration range. 780 nm infrared light was irradiated to the ICG sample to observe the fluorescence expression through a near infrared (NIR) camera. The wavelength is measured by using a spectrum instrument (ocean view) to observe the fluorescence expression wavelength of 811nm. As a result of analyzing the mol concentration according to each sample, the fluorescence expression range was found to be 16.15-0.16M and the optimum fluorescence concentration on the brightest part was found to be 3.23-0.81M.

• Journal of Plant Biology
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• 제39권3호
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• pp.173-177
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• 1996

A novel calcium binding protein (CaBP) was purified to electrophoretic homogeneity from Dunaliella salina. In the course of purification experiment, this CaBP was identified as a monomer and its molecular weight was about 21 kDand isoelectric point (pI) value was about 4.1 using isoelectrofocusing. This CaBP was able to bind Ca2+ even in the pressence of an excess MgCl2 and KCI both in solution. In the SDS-PAGE, the Ca2+-bound form was slower than the Ca2+-free form in the nondenaturing PAGE. This means that the CaBP undergoes conformational change in the Ca2+-bound condition. Furthermore, UV absorption spectrum and fluorescence intensity of this CaBP was investigated. UV absorption peak was appeared at about 258 nm and decreased somewhat in Ca2+-bound condition. In the measurement of fluorescence, maximum intensity was appeared at 303 nm and decreased in Ca2+-bound state, similarly as UV absorption spectrum. These show distinct changes upon Ca2+-binding, which indicate of structural and/or dynamic changes largely reminiscent of other members of the EF-hand Ca2+-binding protein family.

• BMB Reports
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• 제29권3호
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• pp.230-235
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• 1996

The interaction of ${\alpha}-ketoglutarate$ dehydrogenase complex (${\alpha}-KGDC$) with a hydrophobic fluorescent probe [1,1'-bi(4-aniline)naphthalene-5,5'-disulfonic acid] (bis-ANS) was studied. The punfied ${\alpha}-KGDC$ was potently inhibited by bis-ANS with an apparent half maximal inhibitory concentration ($IC_{50}$) of 9.8 ${\mu}m$ at pH 8.0. The catalytic activities of both the E1o and E2o subunits were predominantly inhibited while that of the E3 component was hardly affected. The binding of bis-ANS to the enzyme caused a marked enhancement and blue shift from 523 nm to 482 nm in the fluorescence emission spectrum. The dissociation constant ($K_d$) and the number of binding sites (n) were calculated to be 0.87 mM and 158, respectively. Allosteric regulators such as purine nucleotides and divalent cations further increased the fluorescence intensity of the $bis-ANS-{\alpha}-KGDC$ binary complex. These data suggest that the binding of these allosteric regulators to ${\alpha}-KGDC$ may cause the conformational changes in the enzyme and that bis-ANS could be used as a valuable probe to study the interaction of the multi-enzyme complex and its allosteric regulators.

• 폴리머
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• 제38권4호
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• pp.535-543
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• 2014

이 논문은 napthalimidopropylacrylate(NIPA)와 glycidylmethacrylate(GMA) 공중합체의 합성과 물성에 관한 연구로서 공중합체의 조성, 단량체 반응성비, 공명효과(Q)와 극성효과(e), 나프탈렌발색단을 가진 물질의 형광특성 등을 조사하였다. Azobisisobutyronitronitryl(AIBN) 개시제와 dimethylformamide(DMF) 용매를 $60^{\circ}C$에서 공중합하였다. 단량체의 반응성비는 Fineman-Ross(F-R), Kelen-$T{\ddot{u}}d{\ddot{o}}s$(K-T) 법으로 단량체 반응성비를 구한바, $r_1$은 $r_2$보다 크게 나타났다. NIPA이 GMA보다 공중합의 형성이 더 많음을 알 수 있다. 단량체 반응성비의 곱($r_1{\cdot}r_2$)이 1보다 적어서 불규칙한 교대공중합체가 형성되었고 다른 단량체끼리 결합하게 된 교호공중합체를 형성된 것으로 간주된다. 380 nm에서 약한 분자 형광띠와 460 nm에서 강한 중합체 엑시머 형광띠가 나타났다. NIPA 단량체의 형광수명은 실온에서 UV 355 nm 파장에서 형광붕괴커브를 나타냈으며 $5.1449{\times}10^{-7}s$로 나타났다. GMA 공단량체를 아크릴계에 공중합시켜 우수한 내열성 열경화성 접착제나 코팅제에 응용될 것으로 사료된다.