• 한국기계가공학회지
• /
• 제11권4호
• /
• pp.39-45
• /
• 2012

In this paper, process conditions are investigated for elimination of the grain coarsening and improved material flow during forging process by both of experiments and FEM analysis. Particular interest has been given to understand role of preform shape on the grain coarsening behavior and magnitude of the hammer forging load. As the results of FEM simulation by using DEFORM-3D, the simulated forging loads were 2,200ton in the case of a machined bar which is machined from 65mm to 60mm diameter, and below 1,900ton in the case of machined preform, respectively. The use of preform has been beneficial for reduction of the forging load and elimination of the grain coarsening. However, in the case of as received bar and the round bar, which was machined to 2.5mm thickness in surface layer, some degree of local grain coarsening behavior has been observed. The optimized preform shape could be properly designed by applying the FEM simulation.

• 소성∙가공
• /
• 제1권1호
• /
• pp.52-65
• /
• 1992

In this study a new simplified three-dimensional numerical method and the associated computer program have been developed to simulate the non-axisymmetric extrusion processes. The two-dimensional rigid-plastic finite element method under the generalized plane-strain condition is combined with the slab method. To define the die geometry for a non-axisymmetric extrusion. area mapping technique was used. Streamlined die surface was used to minimize the total extrusion pressure. Extrusion of square, hexagonal and 'T' section from round billet have been simulated and experimented with a model material. The computed results were in good agreement with the experiments in cross-sectional grid distortion. Computational results will be valuable for designing tool geometries and corresponding processes.

• 대한용접접합학회:학술대회논문집
• /
• 대한용접접합학회 2005년도 춘계학술발표대회 개요집
• /
• pp.91-93
• /
• 2005

Forming sheet metal by laser-induced thermal stresses (laser forming) has been extensively studied, and the research has focused on two-dimensional geometries using a multi-pass straight line scan. Recently there came out some useful studies or three-dimensional laser forming which is applied to doubly curved shapes. The task of 3D laser forming sheet metal is to determine a set of process parameters such as laser scanning paths, laser power and scanning speed that will make a given shape. New method for laser forming of a doubly curved surface by using geometrical information was proposed and verified by experiments. This method shows good performance in the sense of calculation time and accuracy compared to the inherent strain method.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2003년도 하계학술대회 논문집 C
• /
• pp.1436-1438
• /
• 2003

In this paper thermal and mechanical properties of elastic epoxy for applying high voltage products were investigated. Glass transition temperature(Tg) of elastic epoxies can't find form room temperature to $200^{\circ}$ by DSC. It occurred weight reduce on $285^{\circ}$ and $451^{\circ}$ by thermo-gravimeter. The first temperature was effected on addictives and the other was epoxy's character. Max, tensile strain showed $28.3kgf/cm^2$ at 20% of mechanical stress in addictives 35phr. SEM micrograph of the fracture surface observed in void and tearing of elastic epoxy at addictives 35phr. The other side, SEM micrograph of rigid epoxy showed the trace which broke.

• International Journal of Oral Biology
• /
• 제33권4호
• /
• pp.179-186
• /
• 2008

Several lines of evidence suggest that osteocytes play a critical role in bone remodeling. Both healthy and apoptotic osteocytes can send signals to other bone surface cells such as osteoblasts, osteoclasts, osteoclast precursors, and bone lining cells through canalicular networks. Osteocytes responding to mechanical strain may also send signals to other cells. To determine the role for osteocytes an mechanical strain in bone remodeling, we examined the effects of fluid flow shear stress on osteoclast precursor cell and osteoblast proliferation and recruitment induced by osteocytes. In addition, the effects of fluid flow shear stress on osteocyte M-CSF, RANKL, and OPG mRNA expression were also examined. MLO-Y4 cells were used as an in vitro model for osteocytes, RAW 264.7 cells and MOCP-5 cells as osteoclast precursors, and 2T3 cells as osteoblasts. MLO-Y4 cells conditioned medium (Y4-CM) was collected after 24h culture. For fluid flow experiments, MLO-Y4 cells were exposed to 2h of pulsatile fluid flow (PFF) at 2, 4, 8, $16{\pm}0.6\;dynes/cm^2$ using the Flexcell $Streamer^{TM}$ system. For proliferation assays, MOCP-5, RAW 264.7, and 2T3 cells were cultured with control media or 10-100% Y4 CM. Cells were cultured for 3d, and then cells were counted. RAW 264.7 and 2T3 cell migration was assayed using transwells with control media or 10-100% Y4-CM. M-CSF, RANKL and OPG in MLO-Y4 mRNA expression was determined by semiquantitative RT-PCR. Y4-CM increased osteoclast precursor proliferation and migration, but decreased 2T3 cell proliferation and migration. CM from MLO-Y4 cells exposed to PFF caused decreased RAW 267.4 cell proliferation and migration and 2T3 migration compared to control Y4-CM. However, Y4-CM from cells exposed to PFF had no effect on 2T3 osteoblastic cell proliferation. PFF decreased RNAKL mRNA and increased OPG mRNA in MLO-Y4 cells compared to control(without PFF). PFF had no effect on M-CSF mRNA expression in MLO-Y4 cells. These results suggest that osteocytes can regulate bone remodeling by communication with osteoclast precursors and osteoblasts and that osteocytes can communicate mechanical signals to other cells.

• 대한기계학회논문집A
• /
• 제26권8호
• /
• pp.1672-1679
• /
• 2002

This paper provides an engineering J estimation equation for cylinders with finite internal axial surfacecracks under internal pressure. The proposed equation is the J estimation equation based on deformation plasticity using Ramberg-Osgood (R-O) materials. Based on detailed 3-D FE results using deformation plasticity, plastic influence functions for fully plastic J components are tabulated for practically interesting ranges of the mean radius-to-thickness ratio, the crack depth-to-length ratio, the crack depth-to-thickness ratio. the strain hardening index for the R-O material, and the location along the semi-elliptical crack front. Based on tabilated plastic influence functions, the J estimation equation along the crack front is proposed and validated for R-O materials. Good agreements between the FE results and the proposed J estimation provide confidence in the use of the proposed method to elastic-plastic fracture mechanics of pressurized piping.

• 한국정밀공학회지
• /
• 제20권6호
• /
• pp.222-229
• /
• 2003

The purpose of this study is to evaluate the mechanical properties of 6061 Al foams, which were fabricated by P/M and multi-step induction heating method, and to build the database, which is needed for computer aided modeling or foam components design. Aluminium foams, consisting of solid aluminium and large quantities of porosities, is widely used in automotive, aerospace, naval as well as functional applications because of its high stiffness at very low density, high impact energy absorption, heat and fire resistance, and greater thermal stability than any organic material. In this study, 6061 Al foams were fabricated for variation of fraction of porosities (%) according to porosities (%)-final heating temperature ( $T_{a3}$) curves. Mechanical properties such as compressive strength, energy absorption capacity, and efficiency were investigated to evaluate the feasibility of foams as crash energy absorbing components. Moreover, effect of the surface skin thickness on plateau stress and strain sensitivity of the 6061 Al foams with low porosities (%) were studied.d.

• 한국정밀공학회지
• /
• 제20권8호
• /
• pp.205-212
• /
• 2003

In this study, in order to investigate a modeling technique of the structure with bolted joints, four kinds of finite element model are introduced; a solid bolt model, a coupled bolt model, a spider bolt model, and no bolt model. All proposed models take account on prestrained effect and contact behavior of flanges to be joined. Among these models, a solid bolt model, which is modeled by using a 3-D solid element and a surface-to-surface contact element between the head/nut and the flange interfaces, has the best accurate responses compared with the experimental results. In addition, coupled bolt model, which couples the degree of freedom between the head/nut and the flange, shows the best effectiveness and usefulness in view of computational time and memory usage. Finally, the bolt model proposed here is adopted for structural analysis of a large diesel engine of a ship consisting of several parts which is connected by long stay bolts.

• 한국결정성장학회지
• /
• 제9권4호
• /
• pp.387-395
• /
• 1999

The strain, surface and interfacial energies of III-V ternary systems were calculated for three kinds of structure modes: the Frank-van der Merwe(FM) mode, the Stanski-Krastanov(SK) mode and the Volmer-Weber(VW) mode. The free energy for each mode was estimated as functions of thickness and composition or lattice misfit. Through comparison of the free energy of each mode, it was found that the thickness-composition phase diagrams of III-V ternary systems can be determined only by considering the balance of the free energy and three kinds of structure modes appear in the phase diagrams. The SK mode appears only when the lattice misfit is large and/or the lattice layer is thick. The most stable structure of the SK mode is a cluster with four lattice layers or minimum thickness on a wetting layer of increasing lattice layers. The VW mode appears when the lattice misfit is large and the lattice layer is thin and only in the INPSb/InP and GaPSb/GaP system which have the largest lattice misfit of III-V ternary systems. The stable region of the SK mode in the GaPSb/GaP and InPSb/InP phase diagrams is largest of all because the composition dependence of the strain energy of these systems is stronger than that of the other systems. The critical number of lattice layers below which two-dimensional(2D) layers precede the three-dimensional(3D) nucleation in the SK mode at x=1.0 depends on the lattice misfit.

• Journal of Microbiology and Biotechnology
• /
• 제17권2호
• /
• pp.207-217
• /
• 2007

The Saccharomyces0 cerevisiae KNU5377 strain, which was isolated from spoilage in nature, has the ability to convert biomass to alcohol at high temperatures and it can resist against various stresses [18, 19]. In order to understand the defense mechanisms of the KNU5377 strain under menadione (MD) as oxidative stress, we used several techniques for study: peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) followed by two-dimensional (2D) gel electrophoresis, liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), and surface-enhanced laser desorption ionization-time of flight (SELDI-TOF) technology. Among the 35 proteins identified by MALDI-TOF MS, 19 proteins including Sod1p, Sod2p, Tsa1p, and Ahp1p were induced under stress condition, while 16 proteins were augmented under normal condition. In particular, five proteins, Sod1p, Sod2p, Ahp1p, Rib3p, Yaf9p, and Mnt1p, were induced in only stressed cells. By LC-ESI-MS/MS analysis, 37 proteins were identified in normal cells and 49 proteins were confirmed in the stressed cells. Among the identified proteins, 32 proteins were found in both cells. Five proteins including Yel047cp and Met6p were only upregulated in the normal cells, whereas 17 proteins including Abp1P and Sam1p were elevated in the stressed cells. It was interesting that highly hypothetical proteins such as Ynl281wp, Ygr279cp, Ypl273wp, Ykl133cp, and Ykr074wp were only expressed in the stressed cells. SELDI-TOF analysis using the SAX2 and WCX2 chips showed that highly multiple-specific protein patterns were reproducibly detected in ranges from 2.9 to 27.0 kDa both under normal and stress conditions. Therefore, induction of antioxidant proteins, hypothetical proteins, and low molecular weight proteins were revealed by different proteomic techniques. These results suggest that comparative analyses using proteomics might contribute to elucidate the defense mechanisms of KNU5377 under MD stress.