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Ralstonia pickettii Enhance the DDT Biodegradation by Pleurotus eryngii

  • Purnomo, Adi Setyo;Maulianawati, Diana;Kamei, Ichiro
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1424-1433
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    • 2019
  • DDT is a hydrophobic organic pollutant, which can be bio-accumulated in nature and have adverse consequences on the physical condition of humans and animals. This study investigated the relationship between the white-rot fungus Pleurotus eryngii and biosurfactant-producing bacterium Ralstonia pickettii associated with the degradation of DDT. The effects of R. pickettii on fungal development were examined using in vitro confrontation assay on a potato dextrose agar (PDA) medium. R. pickettii culture was added to the P. eryngii culture at 1, 3, 5, 7, and 10 ml ($1ml{\approx}1.44{\times}10^{13}CFU$). After 7 d incubation, about 43% of the initial DDT ($12.5{\mu}M$) was degraded by the P. eryngii culture only. The augmentation of 7 ml of R. pickettii culture revealed a more highly optimized synergism with DDT degradation being approximately 78% and the ratio of optimization 1.06. According to the confrontational assay, R. pickettii promoted the growth of P. eryngii towards the bacterial colony, with no direct contact between the bacterial cells and mycelium (0.71 cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-bis(4-chlorophenyl) ethylene) were identified as metabolic products, indicating that the R. pickettii could enhance the DDT biodegradation by P. eryngii.

Solid Electrolyte Technologies for Next-Generation Lithium Secondary Batteries (차세대 리튬이차전지용 고체 전해질 기술)

  • Kim, K.M.;Oh, J.M.;Shin, D.O.;Kim, J.Y.;Lee, Y.G.
    • Electronics and Telecommunications Trends
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    • v.36 no.3
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    • pp.76-86
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    • 2021
  • Technologies for lithium secondary batteries are now increasingly expanding to simultaneously improve the safety and higher energy and power densities of large-scale battery systems, such as electric vehicles and smart-grid energy storage systems. Next-generation lithium batteries, such as lithium-sulfur (Li-S) and lithium-air (Li-O2) batteries by adopting solid electrolytes and lithium metal anode, can be a solution for the requirements. In this analysis of battery technology trends, solid electrolytes, including polymer (organic), inorganic (oxides and sulfides), and their hybrid (composite) are focused to describe the electrochemical performance achievable by adopting optimal components and discussing the interfacial behaviors that occurred by the contact of different ingredients for safe and high-energy lithium secondary battery systems. As next-generation rechargeable lithium batteries, Li-S and Li-O2 battery systems are briefly discussed coupling with the possible use of solid electrolytes. In addition, Electronics and Telecommunications Research Institutes achievements in the field of solid electrolytes for lithium rechargeable batteries are finally introduced.

Biosorption of Cr (VI) ions by Ficus religiosa barks: Batch and continuous study

  • Karthick, S;Palani, R;Sivakumar, D;Meyyappan, N
    • Membrane and Water Treatment
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    • v.13 no.5
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    • pp.209-217
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    • 2022
  • In the present research, Ficus religiosa Bark (FRB) is used as an adsorbent for the removal of heavy metal Cr (VI) ions. This Ficus religiosa Bark was characterized by Scanning Electron Microscope, Fourier transform infrared Spectroscopy, Thermo Gravimetric Analyzer and the results showed that activated adsorbent have high adsorption capacity and withstand even in high temperature. Batch and Continuous experiments were conducted to determine the effect of various parameters such as pH, contact time, adsorbent dose and initial metal concentration. The biosorption followed pseudo first order kinetic model. The adsorption isotherms of Cr (VI) on Ficus religiosa fitted well with the Temkin model. In Batch study, maximum biosorption capacity of Cr (VI) was found to be 37.97 mg g-1 (at optimal pH of 2, adsorbent dosage of 0.3 grams and concentration of Cr (VI) is100 mg L-1). The Continuous mode of study shows that 97% of Cr (VI) ion removal at a flow rate of 15 ml min-1. From the results, selected Ficus religiosa Bark has the higher adsorption capacity for the removal of Cr (VI) ions from wastewater.

Design and Implementation of Physical Computing Education Content based on Augmented Reality

  • Kim, So-Young;Jung, Eunmi;Kim, Heesun
    • International Journal of Internet, Broadcasting and Communication
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    • v.14 no.4
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    • pp.198-205
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    • 2022
  • Along with a variety of coding education, physical computing education for controlling various sensors is being actively conducted for elementary, middle, and high school students in line with the era of the fourth industrial revolution. A problem with physical computing education using Arduino is pin connection errors between Arduino and various sensors. Most of the students who come into contact with the Arduino for the first time often do not know the purpose of the Arduino pin and the connection position of the pin. Also, hardware built with incorrect pin connections to the Arduino board often does not work properly. If this case continues, students will lose interest in coding education. Therefore, in this paper, we implemented an augmented reality application that informs the connection process of the Arduino board and the sensor during physical computing coding education using Arduino, and designed and implemented educational content for the Arduino pin position and connection process. First, we explain the role of the Arduino board and the sensor and the location of the pins. After that, the students run the educational augmented reality educational content using their smartphones and check the correct pin connection process between the Arduino and the sensor. In the physical computing education, augmented reality content is used to increase the understanding and immersion of the class. It is expected that the educational effect will also increase by inducing fun and interest in physical computing coding education.

Surface Quality of Products according to the Material and Coating Condition of the Forming Tool in Incremental Sheet Forming (점진성형공구 코팅처리 및 소재에 따른 성형품 표면품질 분석)

  • H. W. Youn;N. Park
    • Transactions of Materials Processing
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    • v.32 no.6
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    • pp.360-366
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    • 2023
  • This study is concerned with the surface quality of products according to the material and coating condition of the forming tool in incremental sheet forming. Three forming tools, SKD11 with and without diamond-like-coating (DLC) and polymer tool tip, were used to form conical and pyramidal geometries to take into account the influence of friction between the forming tool and the sheet on the surface quality including geometric accuracy of deformed samples. Each test was performed using SUS304 with a thickness of 0.4 mm according to different incremental depths per lap of 0.5 mm, 1.0 mm, and 1.5 mm for the contour tool path, considering the increase in normal force which is associated with the frictional behavior during local deformation. The surface quality was then investigated through surface roughness measured with KEYENCE VR-6000 and relative strain distribution including deformed shape analyzed with ARGUS which is a non-contact optical strain measurement system. Differences between 3D CAD surfaces and captured geometry from experiments were evaluated to compare the effect of friction on geometric accuracy. From comparisons of experimental results, it was revealed that the polymer-based tool tip can improve surface quality and geometric accuracy by reducing the undesired material flow due to local friction in the increment sheet forming process.

Development of scaling approach based on experimental and CFD data for thermal stratification and mixing induced by steam injection through spargers

  • Xicheng Wang;Dmitry Grishchenko;Pavel Kudinov
    • Nuclear Engineering and Technology
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    • v.56 no.3
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    • pp.1052-1065
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    • 2024
  • Advanced Pressurized Water Reactors (APWRs) and Boiling Water Reactors (BWRs) employ a suppression pool as a heat sink to prevent containment overpressure. Steam can be discharged into the pool through multi-hole spargers or blowdown pipes in both normal and accident conditions. Direct Contact Condensation (DCC) creates sources of momentum and heat. The competition between these two sources determines the development of thermal stratification or mixing of the pool. Thermal stratification is of safety concern as it reduces the cooling capability compared to a completely mixed pool condition. In this work we develop a scaling approach to prediction of the thermal stratification in a water pool induced by steam injection through spargers. Experimental data obtained from large-scale pool tests conducted in the PPOOLEX and PANDA facilities, as well as simulation results obtained using validated codes are used to develop the scaling. Two injection orientations, namely radial injection through multi-hole Sparger Head (SH) and vertical injection through Load Reduction Ring (LRR), are considered. We show that the erosion rate of the cold layer can be estimated using the Richardson number. In this work, scaling laws are proposed to estimate both the (i) transient erosion velocity and (ii) the stable position of the thermocline. These scaling laws are then implemented into a 1D model to simulate the thermal behavior of the pool during steam injection through the sparger.

A Study on Prediction of Sedimentation Efficiency for Sedimentation Basin using Lagrangian Method (침전지의 유동 특성과 Lagrangian Method를 이용한 침전효율 예측에 관한 연구)

  • Choi, Jong-Woong;Hong, Sung-Taek;Kim, Seong-Su;Kim, Youn-Kwon;Park, No-Suk
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.5
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    • pp.229-236
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    • 2017
  • Flow characteristics analysis and tracer test simulations for the rectangular typed sedimentation basins, which have been operated at D_water treatment plant in Korea, were carried out using CFD (Computational Fluid Dynamics) techniques for design ($15,864m^3/day$) and operation flowrate ($33,333m^3/day$). Also, each efficiency of the sedimentation basin was evaluated by application of the Lagrangin technique on the assumption of the particles flowing into the inlet of the sedimentation basin. From the results of simulation, the mean velocity values for making the flow in the settling basin as a plug flow region were derived as 0.00193 m/s and 0.00417 m/s, respectively. In addition, ${\beta}$ (effective contact factor) values were calculated to be 0.51 and 0.46, and the Morrill Index values were 6.05 and 3.21, respectively for both flowrate conditions.

3-Dimensional Analysis of the Running Motion in the Max-Velocity Phase and the Fatigue Phase During 400m Sprint by Performed Elementary School Athletes (달리기시 최고 속도 및 피로 구간의 3차원 동작 분석)

  • Bae, Sung-Jee
    • Korean Journal of Applied Biomechanics
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    • v.16 no.4
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    • pp.115-124
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    • 2006
  • This study was conducted to investigate the running motion in the max-velocity phase(150-160m) and the fatigue phase(350-360m) during 400m sprint by performed elementary school athletes. Eighteen elementary school male athletes who achieved at least the 3rd place in the sprint at the Korea Gangwon-Do elementary school track and field meetings during 2004 and 2005 were selected as subjects. The running motions performed by the subjects were recorded using two 8mm high speed cameras at the nominal speed of 100 frames per second. The Direct Linear Transformation technique was adopted from the beginning of filming to the final stage of data extraction. KWON 3D motion analysis package program was used to compute the 3 Dimensional coordinates, smoothing factor in which lowpass filtering method was used and cutoff frequency was 6.0 Hz. The movement patterns during foot touchdown and takeoff for the running stride were related with the biomechanical consideration. Within the limitations of this study it is concluded: In order to increase running velocity, several conditions must be fullfilled at the instant of leg touchdown and takeoff during the fatigue phase(350-360m). First, the body C.O.G(Center of Gravity) height should be raised at the instant of leg touchdown and takeoff during the fatigue phase. Second, the foot contact time should be shortened and the takeoff distance should be increased at the foot takeoff during the fatigue phase. Third, the shank angular velocity with respect to a transverse axis through the center of gravity should be increased during the leg touchdown and takeoff in the fatigue phase. Forth, the active landing style described as clawing the ground with the sole of the foot should be performed during the leg touchdown and takeoff in the fatigue phase) phase. Fifth, In order to increase running velocity in the fatigue phase while taking a slightly greater leg knee angle and body lean angle within the range of the subject's running motion during the fatigue phase would result in greater flight distance.

Non-restraint Master Interface of Minimally Invasive Surgical Robot Using Hand Motion Capture (손동작 영상획득을 이용한 최소침습수술로봇 무구속 마스터 인터페이스)

  • Jang, Ik-Gyu
    • Journal of Biomedical Engineering Research
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    • v.37 no.3
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    • pp.105-111
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    • 2016
  • Introduction: Surgical robot is the alternative instrument that substitutes the difficult and precise surgical operation; should have intuitiveness operationally to transfer natural motions. There are limitations of hand motion derived from contacting mechanical handle in the surgical robot master interface such as mechanical singularity, isotropy, coupling problems. In this paper, we will confirm and verify the feasibility of intuitive Non-restraint master interface which tracking the hand motion using infra-red camera and only 3 reflective markers without the hardware handle for the surgical robot master interface. Materials & methods: We configured S/W and H/W system; arranged 6 infra-red cameras and attached 3 reflective markers on hands for measuring 3 dimensional coordinate then we find the 7 motions of grasp, yaw, pitch, roll, px, py, pz. And we connected Virtual-Master to the slave surgical robot(Laparobot) and observed the feasibility. To verify the result of motion, we compare the result of Non-restraint master and that of clinometer (and protractor) through measuring 0~180 degree, 10degree interval, 1000 samples and recorded standard deviation stands for error rate of the value. Results: We confirmed that the average angle values of Non-restraint master interface is accurately corresponds to the result of clinometer (and protractor) and have low error rates during motion. Investigation & Conclusion: In this paper, we confirmed the feasibility and accuracy of 3D Non-restraint master interface that can offer the intuitive motion of non-contact hardware handle. As a result, we can expect the high intuitiveness, dexterousness of surgical robot.

Characterization and Seawater Filtration Performance of Commerical Microfiltration and Ultrafiltration Membranes (상업용 정밀여과/한외여과막의 특성 분석 및 해수 여과 성능 평가)

  • Choi, Changkyoo;Kim, In S.
    • Journal of Korean Society of Environmental Engineers
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    • v.39 no.9
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    • pp.542-547
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    • 2017
  • This paper was to analyze the membrane characterization of hydrophilicity, surface morphology and membrane chemical anlysis of three commercial microfiltration/ultrafiltration membranes, and evaluate the filtration performance of a seawater to assess the availability for pretreatment of desalination process. From the results of contact angle, Mem-3, fabricated with polyacrylonitrile, was highly hydrophilic. It find out that Mem-3 has more anti-biofouling property. In Field emission scanning electron microscope (FESEM), Mem-1 (polyethylene) and Mem-2 (Polyvinylidenefluoride) showed the sponge-like shape and Mem-3 showed finger-like shape. Membrane chemical analysis by energy dispersive spectrometer (EDS) presented that Mem-2 was mostly fluoride and Mem-3 had s high ratio of N (32.47%) due to the nitrile group. The permeation flowrate per time on suction pressures using deionized water (D.I. water) tends that permeation rate of Mem-3 more increased when the pressure was increased compared to other membranes. From the results of turbidity and total suspended solids (TSS) removal, turbidity of permeate was 0.191 NTU to 0.406 NTU and TSS was 2.2 mg/L to 3.0 mg/L in all membranes, indicating that it was not suitable for the pretreatment of seawater desalination by short-term experiments.