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Volatile Compounds and Antiproliferative Effects of Dendropanax morbifera on HepG2 Cells (황칠나무의 휘발성 화합물 분석 및 HepG2 세포의 증식 억제 효과)

  • Yang, Seun-Ah;Garcia, Coralia V.;Lee, Ji-Won
    • Journal of Life Science
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    • v.27 no.5
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    • pp.561-566
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    • 2017
  • Dendropanax morbifera Lev. is known in Korea for its golden sap and medicinal properties. The many biological activities of the leaf and stem extracts suggest that this tree could be a valuable source of medicinal compounds for the treatment of various ailments such as dermatitis, migraines, dysmenorrhea, muscle pain, and infectious diseases. However, there is little information on the composition and biological activity of the volatile fraction of D. morbifera. Therefore, in this study, the volatile compounds in leaves, stems, and sap of D. morbifera were isolated using solvent and supercritical fluid extraction (SFE), and analyzed by gas chromatography/mass spectrometry to reveal their chemical composition and identify potential compounds of interest. Fifteen compounds were identified in the leaf extracts, whereas 29 and 3 compounds were identified in the stem and sap extracts, respectively. The volatile profiles obtained using solvent and SFE differed. Esters and aromatic hydrocarbons predominated in the solvent extract of leaves and SFE extract of stems, whereas the solvent extract of stems and SFE extract of leaves contained terpenoids. Limonene, ${\alpha}$-pinene, and ${\beta}$-myrcene were identified in the volatile extract of sap, with limonene representing 96.30% of the total peak area. In addition, the antiproliferative effects of the solvent extracts of leaves and stems were evaluated, revealing that these solvent extracts were particularly effective in decreasing the proliferation of HepG2 cells.

Determination of Fission Products in Simulated Nuclear Spent Fuels by Cation.Anion Exchange Chromatography and Inductively Coupled Plasma Atomic Emission Spectrometry (양.음이온교환 크로마토그래피와 유도결합플라스마 원자방출분광법을 이용한 모의 사용후핵연료 중 핵분열생성물 분석)

  • Choi, Kwang Soon;Sohn, Se Chul;Pyo, Hyung Yeol;Suh, Moo Yul;Kim, Do Yang;Park, Yang Soon;Jee, Kwang Yong
    • Analytical Science and Technology
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    • v.13 no.4
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    • pp.446-452
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    • 2000
  • The simulated nuclear spent fuel (SIMFUEL) containing the platinum group elements which will not be dissolved in a nitric acid was completely dissolved with a acid digestion bomb. The metallic elements separated in the SIMFUEL were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES). Because the peaks of metallic elements were spectrally interfered by uranium spectrum, uranium and metallic elements were separated by cation exchange resin for Mo, Pd, Rh and Ru and by anion exchange resin for Ba, Ce, La, Nd, Rh, Sr, Y and Zr, respectively. The recovery of Mo, Pd, Rh and Ru after separation by cation exchange chromatography found to be 99-103% and anion exchange separation showed 96.5-107% of recovery except Y with the simulated solution whose concentration was similar to the spent nuclear fuel. The relative standard deviation of this method showed 1.3-6.7% in the SIMFUEL whose concentrations of metallic elements were between several $10^2-10^3$ppm.

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Evaluation of the Minimum Shear Reinforcement Ratio of Reinforced Concrete Members (철근콘크리트 부재의 최소전단보강근비의 평가)

  • Lee Jung-Yoon;Yoon Sung-Hyun
    • Journal of the Korea Concrete Institute
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    • v.16 no.1 s.79
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    • pp.43-53
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    • 2004
  • The current Korean Concrete Design Code(KCI Code) requires the minimum and maximum content of shear s in order to prevent brittle and noneconomic design. However, the required content of the steel reinforcement In KCI Code is quite different to those of the other design codes such as fib-code, Canadian Code, and Japanese Code. Furthermore, since the evaluation equations of the minimum and maximum shear reinforcement for the current KCI Code were based on the experimental results, the equations can not be used for the RC members beyond the experimental application limits. The concrete tensile strength, shear stress, crack inclination, strain perpendicular to the crack, and shear span ratio are strongly related to the lower and upper limits of shear reinforcement. In this research, an evaluation equation for the minimum content of shear reinforcement is theoretical proposed from the Wavier's three principals of the mechanics of materials.

Reconstruction of the Thumb, the Second and Third Finger in Patient with Amputation of Right Five Fingers (우측 수부의 모든 수지 절단 환자에서 무지와 제 2, 3 수지 재건술)

  • Lee, Jun-Mo;Kim, Gyu-Hyung
    • Archives of Reconstructive Microsurgery
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    • v.10 no.2
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    • pp.143-148
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    • 2001
  • The hand with amputation of all fingers is useless for activities of daily living and traumatic amputation of some of the fingers can result in the diminished ability to perform power grip and precision grip which is vital to maintain normal function of the hand. Precision grip is used to hold an object between the opposable thumb and flexed fingers. In power grip the object is held between the flexed fingers and the palm while the thumb applies the necessary counterpressure to maintain the grip on the object. A 35 year old male lost his right all fingers including thumb at the level of proximal phalanx from the pressure machinary accident. Thumb was reconstructed using wrap around flap and the second and third fingers were reconstructed using the second and third toe transplantation. Seven years after reconstruction, he uses the reconstructed thumb and the second and the third fingers for eating meals, writing down a paper with a pencil and putting on socks.

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The Solvent Extraction of Univalent Cation Picrates by New Podands (새로운 포단드에 의한 피크린산 일가 양이온 염의 용매추출)

  • Jung, Jong Hwa;Cho, Sung Bae;Kim, Jineun;Kim, Jae Sang;Lee, Shim Sung
    • Analytical Science and Technology
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    • v.6 no.1
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    • pp.29-37
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    • 1993
  • Some new podands containing phenyl(B), benzyl(Bz), pyridine(Py), quinoline(Q) and naphthalene(Np) as end-groups, and oxygen(O) and sulfur(S) in ether chains as donor atoms have been synthesized. The univalent cation binding characteristics of these podands have been studied by NMR titration and solvent extraction. By NMR titration we have found that the most of podands form 1:1 complexes with $Ag^+$ ion. Especially, the substituted sulfur atoms in ether chains show the effects to enhance the stabilities. We also carried out the extractions of univalent cation picrates including alkaline metal, $Ag^+$, $Tl^+$ and $NH_4{^-}$ ions from aqueous to chloroform layer by using these podands. We found that the extractabilities of $Ag^+$ ion with the quinoline-containing podands such as, $Q_2O_4$, $Q_2O_5$ and $BQO_5$ were 86.8, 86.6 and 48.0% respectively, but the naphthalene-containing podands such as, $Np_2O_4$ and $Np_2O_5$ extracted quite small amount. Otherwise, in cases of $Bz_2O_3S_2$(89.4%), $B_2O_2S_2$(96.8%), $B_2O_3S_2$(58.9%), $Py_2O_2S_2$(58.8%), $Py_2O_3S_2$(42.1%), and $B_2O_4S$(15.0%), interestingly, $Bz_2O_3S_2$ which have sulfur atoms and benzyl groups showed the highest extraction selectivity for $Ag^+$ ion. This result seems due to not only the strong interaction of $Ag^+$ ion with sulfur donors according to the HSAB theory, but also the effective ${\pi}-{\pi}$ stacking interaction between two aromatic end-groups which is enhanced by the flexible methylene spacing group in benzyl groups instead of phenyl groups. The extraction coefficients gave the similar tendency as the extractabilities and the stabilities. From these results, it could be concluded that the predominant factor affected to extraction coefficients is the stabilities, which are strongly influenced by the structures of podands.

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Incidence and Associated Factors of Delirium after Orthopedic Surgery (정형외과 수술 후 발생한 섬망의 발생 빈도와 관련 인자)

  • Lee, Si-Wook;Cho, Chul-Hyun;Bae, Ki-Cheor;Lee, Kyung-Jae;Son, Eun-Seok;Um, Sang-Hyun
    • Journal of the Korean Orthopaedic Association
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    • v.54 no.2
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    • pp.157-163
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    • 2019
  • Purpose: To investigate the incidence and associated factors of delirium after orthopedic surgery. Materials and Methods: A total of 2,122 cases, who were older than 20 years and underwent orthopedic surgery at a single medical center during a one year period were included. Among them, 132 patients who were diagnosed with delirium after surgery under the Diagnostic and Statistical Manual of Mental Disorders-V criteria and medicated under the consultation of a psychiatrist were included in the study The differences in the incidence of delirium and several affecting factors were analyzed. Results: The overall incidence of delirium after surgery was 6.2% (132 in 2,122 cases). The mean age of the delirium group was 77.4 years (range, 54-92 years), which was higher than that of the non-delirium group (58.1 years). The percentage of women in the delirium group was 63.6% (84 in 132 cases), which was higher than that of the women in the non-delirium group (49.0%). The incidence of delirium after surgery was 9.3% (85 in 916 cases) due to trauma and 3.9% (47 in 1206 cases) due to disease. The incidence of postoperative delirium according to the surgical region was 29.2% (7 in 24 cases) in two or more regions, 13.7% (72 in 526 cases) in the hip, and 9.6% (14 in 146 cases) in the spine, 3.5% (20 in 577 cases) in the knee-lower leg, 2.5% (5 in 199 cases) in the foot-ankle, 2.4% (11 in 457 cases) in the shoulder-elbow, and 1.6% (3 in 189 cases) in the forearm-wrist-hand. Delirium occurred more rapidly in women and surgery due to disease, and the duration of delirium was longer in patients with dementia and major depressive disorders. Conclusion: The incidence of postoperative delirium was high in cases of surgery due to trauma and in cases of surgery in two or more sites. The incidence of postoperative delirium according to a single surgical region was higher in the order of the hip, spine, and knee. Active intervention is needed regarding the correctable risk factor.

Characteristics of Groundwater, Sewage Water and Stream Water Contamination Based on VOCs Concentration Around Ulsan, Korea (울산지역 지하수, 하수 및 하천수의 VOCs 오염특성)

  • Cho, Byong-Wook;Yun, Uk;Im, Hyun-Chul;Sung, Ig-Hwan;Jang, Woo-Seog
    • Economic and Environmental Geology
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    • v.38 no.1
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    • pp.57-65
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    • 2005
  • To investigate the characteristics of the volatile organic compounds (VOCs) concentration in the groundwater around Ulsan, Korea, 168 groundwaters, 12 stream waters, and 6 sewage waters were analyzed for 61 VOCs. Results showed that VOCs were not detected in stream waters and total VOCs concentration in 5 sewage waters was in the range of ND-22.3 ${\mu}$g/L. In 78 groundwater samples more than one VOCs were detected and VOCs concentration of the samples ranged from 0.1 ${\mu}$g/L to 387.1 ${\mu}$g/L. However, VOCs concentration of 66 samples out of 78 samples showed less than 10 ${\mu}$g/L and that of only 6 samples exceeded Korea drinking water limit (KDWL). 42 VOCs detected from the 168 groundwaters were 14 aromatic hydrocarbons out of 25, 27 chlorinated aliphatic hydrocarbons out of 35, and methyl tert-butyl ether (MTBE). Detection rate of each VOCs in the groundwaters was as follows: chloroform in 43 samples (25.6%), methylene chloride in 36 samples (21.4%), TCE in 26 samples (15.5%), 1,1-dichloroethane in 19 samples (1.3%), PCE in 16 samples (9.5%), cis-1,2-DCE in 15 samples (8.9%), and toluene in 14 samples (8.3%). Even though VOCs concentration in the groundwaters of the study area is still low, the city is expanding and the drinking water limit is becoming strict, and therefore continuous monitoring is necessary.

Characteristics of Biosurfactant Producing Pseudomonas sp. HN37 (생물계면활성제 생성 세균 Pseudomonas sp. HN37의 특성)

  • Jung, Da Hee;Chang, Dong Ho;Kim, Yeong Eun;Jeong, Mi Rang;Hahn, Kyu Woong;Kim, Hyong Bai;Park, Kyeong Ryang
    • Korean Journal of Microbiology
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    • v.50 no.1
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    • pp.33-39
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    • 2014
  • One hundred forty four bacterial colonies which were able to degrade crude oil were isolated from soil samples that were contaminated with oil in Daejeon area. Among them, one bacterial strain was selected for this study based on its emulsification activity, growth rate and surface tension activity, and this selected bacterial strain was identified as Pseudomonas sp. HN37 through physiological- biochemical tests and analysis of its 16S rRNA sequence. Pseudomonas sp. HN37 utilize the several aliphatic hydrocarbons, 3,5-dinitrosalicylic acid and 2,4-dichlorophooxyacetic acid as a sole carbon source. And this bacterial strain showed a high resistance to antibiotics such as ampicillin and chloramphenicol, as well as heavy metals such as Ba, Cr, Li and Mn. Also, it was found that the optimal pH and temperature for the cell growth, surface tension, and emulsification activity of Pseudomonas sp. HN37 were pH 6.0-9.0 and $30^{\circ}C$, respectively. The emulsification and surface tension activity was reached the maximum to 1% (V/V) crude oil and 1% (W/V) NaCl concentration. The surface tension of the culture broth was decreased from 62 to 27 dyne/cm after fifteen hours of inoculation in LB media.

Aromatic Constituents and Essential Oil Content of Codonopsis lanceolata Trautv Cultivated at Different Altitudes (표고에 따른 더덕의 향기 성분과 정유 함량)

  • Kim, Sang-Kuk;Kang, Dong-Kyoon;Min, Gi-Gun;Chung, Sang-Hwan;Lee, Seong-Phil;Lee, Sang-Chul;Choi, Boo-Sull
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.1
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    • pp.58-62
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    • 1999
  • This study was to determine aromatic constituents and essential oil content in the root of Codonopsis lanceolata grown at different altitudes; Andong(150m), Mt. Hagka(550m) and Mt. Irwol(750m). Soil pH in Mt. Hagka and Mt. Irwol was 5.6 and 6.1, respectively, which was lower than in Andong(150m), but organic matter content was higher than that of Andong with 6.7 to 8.8%. Fresh root yield also increased as cultural region was located at higher altitude. In particular, yield in Mt. Irwol was 281kg/10a. Essential oil content was highest of 0.006% in the Mt. Irwo1. Forty-eight volatile aromatic compounds in the root of Codonopsis lanceolata were identified by GC/MS. Major aromatic compounds were 1-hexanol, cis-3-hexano1. and Trans-2-hexanol. In partucular, Trans-2-hexanol, was highest in Mt. Irwol attaining the 47.22% area. As a result, it was considered that cultural region above 700m in altitude was the most effective for the improvement of essential oil and aromatic constituents in the roots of Condonopsis lanceolata Trautv.

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Conservation Management Methods and Classification Type of Flora in the Bongamsa(Temple) Area, Mungyeong (문경 봉암사 일대의 식물상 유형별 분류 및 보전관리방안)

  • Oh, Hyun-Kyung;Han, Yun-Hee;Choi, Song-Hyun
    • Korean Journal of Environment and Ecology
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    • v.25 no.4
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    • pp.447-469
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    • 2011
  • The flora of Bongamsa (Temple) area, Mungyeong were listed 552 taxa; 99 families, 303 genera, 468 species, 1 subspecies, 70 varieties and 13 forms. Based on the rare plants (IUCN), total 12 taxa; Crypsinus hastatus (Polypodiaceae), Eranthis stellata (Ranunculaceae), Cnidium tachiroei (Umbelliferae), Monotropa hypopithys (Pyrolaceae), Rhododendron micranthum (Ericaceae), Salvia chanroenica (Labiatae), Gastrodia elata (Orchidaceae), etc. Based on the endemic plants, total 11 taxa; Asarum chungbuensis, Aconitum pseudolaeve, Corydalis maculata, Lonicera subsessilis, Aster koraiensis, Saussurea seoulensis, etc. Based on the specific plants by floral region were total 48 taxa (8.7% of all 552 taxa of vascular plants); 2 taxa in class V, 5 taxa (Cimicifuga heracleifolia, Wistaria floribunda, Elsholtzia angustifolia, etc.) in class IV, 10 taxa (Betula davurica, Spiraea chinensis, Acer barbinerve, Vaccinium koreanum, Syringa reticulata var. mandshurica, etc.) in class III, 7 taxa (Potentilla dickinsii, Viola orientalis, Ostericum melanotilingia, Melica nutans, Veratrum parulum, etc.) in class II, 24 taxa (Camptosorus sibiricus, Cephalotaxus koreana, Betula schmidtii, Ilex macropoda, Tilia amurensis, Triadenum japonica, Lobelia sessilifolia, etc.) in class I. Based on the naturalized plants, total 21 taxa (Silene armeria, Oenothera lamarckiana, Symphytum officinale, Erechtites hieracifolia, etc.) and naturalization rate was 3.8% of all 552 taxa of vascular plants. The flora of importance in the Bongamsa, rare plants were Crypsinus hastatus, Eranthis stellata, Gastrodia elata, Monotropa uniflora, Monotropa hypopithys, Rhododendron micranthum and Cnidium tachiroei, Iris ensata var. spontanea in intermediate marsh and alien species classify into planted species of Bongamsa, area for investigation of afforestation and artificial forests, introduced of naturalized plants.