• Tuberculosis and Respiratory Diseases
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• v.58 no.1
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• pp.31-42
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• 2005

Background : Peroxiredoxins (Prxs) are a relatively newly recognized, novel family of peroxidases that reduce $H_2O_2$ and alkylhydroperoxide into water and alcohol, respectively. There are 6 known isoforms of Prxs present in human cells. Normally, Prxs exist in a head-to-tail homodimeric state in a reduced form. However, in the presence of excess $H_2O_2$, it can be oxidized on its catalytically active cysteine site into inactive oxidized forms. This study surveyed the types of the Prx isoforms present in the pulmonary epithelial, macrophage, endothelial, and other cell lines and observed their response to oxidative stress. Methods : This study examined the effect of exogenous, excess $H_2O_2$ on the Prxs of established cell lines originating from the pulmonary epithelium, macrophages, and other cell lines, which are known to be exposed to high oxygen partial pressures or are believed to be subject to frequent oxidative stress, using non-reducing SDS polyacrylamide electrophoresis (PAGE) and 2 dimensional electrophoresis. Result : The addition of excess $H_2O_2$ to the culture media of the various cell-lines caused the immediate inactivation of Prxs, as evidenced by their inability to form dimers by a disulfide cross linkage. This was detected as a subsequent shift to its monomeric forms on the non-reducing SDS PAGE. These findings were further confirmed by 2 dimensional electrophoresis and immunoblot analysis by a shift toward a more acidic isoelectric point (pI). However, the subsequent reappearance of the dimeric Prxs with a comparable, corresponding decrease in the monomeric bands was noted on the non-reducing SDS PAGE as early as 30 minutes after the $H_2O_2$ treatment suggesting regeneration after oxidation. The regenerated dimers can again be converted to the inactivated form by a repeated $H_2O_2$ treatment, indicating that the protein is still catalytically active. The recovery of Prxs to the original dimeric state was not inhibited by a pre-treatment with cycloheximide, nor by a pretreatment with inhibitors of protein synthesis, which suggests that the reappearance of dimers occurs via a regeneration process rather than via the de novo synthesis of the active protein. Conclusion : The cells, in general, appeared to be equipped with an established system for regenerating inactivated Prxs, and this system may function as a molecular "on-off switch" in various oxidative signal transduction processes. The same mechanisms might applicable other proteins associated with signal transduction where the active catalytic site cysteines exist.

• Geotechnical Engineering
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• v.14 no.3
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• pp.25-46
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• 1998

This paper shows that there are the results of a series of model tests on the behavior of single pipe pile which is subjected to lateral load in, Nak-dong River sand. The purpose of the present paper is to estimate the effect of Non-homogeneity. constraint condition of pile head, lateral load velocity, relative density, and embedded length of pile on the behavior of single pile. These effects can be quantified only by the results of model tests. Also, these are compared with the results of the numerical methods (p-y method, modified Vlasov method; new ${\gamma}$ parameter, Characteristic Load Method'CLM). In this study, a new ${\gamma}$ parameter equation based on the Vlasov method was developed to calculate the modulus of subgrade reaction (E. : nhz.) proportional to the depth. The p-y method of analysis is characterized by nonlinear behavior. and is an effective method of designing deep foundations subjected to lateral loads. The new method, which is called the characteristic load method (CLM). is simpler than p-y analysis. but its results closely approximates p-y analysis results. The method uses dimensional analysis to characterize the nonlinear behavior of laterally loaded piles with respect to be relationships among dimensionless variables. The modulus of subgrade reaction used in p-y analysis and modified Vlasov method obtained from back analysis using direct shear test (DST) results. The coefficients obtained from DST and the modified ones used for the prediction of lateral behavior of ultimate soil reaction range from 0.014 to 0.05. and from 0.2 to 0.4 respectively. It is shown that the predicted numerical results by the new method (CLM), p-y analysis, and modified Vlasov method (new parameter) agree well with measured results as the relative density increases. Also, the characteristic load method established applicability on the Q-Mnu. relationship below y/D=0.2.