• 사상체질의학회지
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• 제4권1호
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• pp.193-212
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• 1992

Comparative hormones quantities on the blood analysis was carried out to investigate the hormones specific characters on the blood in four types of physical constitution. Thyroid-stimulating (TSH), triiodothyronine($T_3$), triiodothyronine uptake($T_3$ uptake), free triiodothyronine(free $T_3$), thyroxine($T_4$), free thyroxine (free $T_4$), thyroxine-binding globulin(TBG), adrenocorticotropic hormone(ACTH), luteinizing hormone(LH), testosterone were measured. The results obtained were summarized as follows: 1. The value of thyroid-stimulating hormone(TSH) on the constitution didn't shown significant difference, while the value of So-EUM-IN was increased and SO-YANG-IN was decreased. 2. The value of triiodothyronine($T_3$) on the constitution didn't shown significant difference, while the value of TAE-EUM-IN was decreased. 3. The value of triiodothyronine uptake($T_3$ uptake) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-EUM-IN, TAE-EUM-In and SO-YANG-IN showed significant difference. 4. The value of free triiodothyronine(free $T_3$) on the constitution didn't shown signifcant difference, but between the value of TAE-EUM-IN and SO-EUM-In, TAE-EUM-In and SO-YANG-IN showed significant difference. 5. The value of thyroxine($T_4$) on the constitution didn't shown significant difference, while the value of TAE-EUM-IN and SO-YANG-IN showed difference. 6. The value of free thyroxine(free $T_4$) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-YANG-IN showed significant difference. 7. The value of thyroxine-binding globulin(TBG) on the constitution didn't shown significant difference. 8. The value of adrenocorticotropic hormone(ACTH) on the constitution didn't shown significant difference. 9. The value of luteinizing hormone(LH) on the constitution didn't shown significant difference, but between the value of TAE-EUM-IN and SO-EUM-IN, TAE-EUM-IN and SO-YANG-IN showed significant difference. 10. The value of testosterone on the constitution didn't shown significant difference.

• Journal of Chest Surgery
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• 제25권11호
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• pp.1209-1217
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• 1992

Triiodothyronine[T3] is an important regulator of the tissue metabolism, and may have potential use as an inotropic agent. The change of serum T3 level was studied in the pediatric age patients after cardiopulmonary by pass. Thyroid function was tested pre-operatively in 33 patients and total triiodothyronine[TT3] levels were serially measured during and after cardiopulmonary bypass[CPB]. After correction of dilutional effect, effects of various factors on the TT3 levels were analyzed. Abrupt fall of TT3 level was demonstrated at 15 minutes after CPB[80.1$\pm$5.9ng/dL] from the initial level of 133.6$\pm$5.3ng/dL, with some recovery at 6 hours[115.4$\pm$6.7ng/dL]. After then, gradual decrease occured reaching to the level of 77.2$\pm$4.2ng /dL at 24 hours. These falls of the TT3 after CPB were statistically significant. [p<0.01 ANOVA] Statistically significant correlation were found between the degree of hemodilution and TT3 concentration at 15 and 30 minutes after CPB[p<0.05]. But, other factors were analyzed to have no effect on TT3 levels. As the degree of the hemodilution increases, TT3 decreased less. This observation probably supports the fact that decrease of TT3 during CPB may be a result of sequestration of T3 into peripheral tissue. Although it was not statistically significant[p=0.08], the fall of TT3 was greater in the group to which plasmanate was added, than those not added. This finding seemed to be due to the increase of albumin and other thyroid-hormone-binding-proteins in the serum. Increase of these binding proteins might potentiate the sequestration of T3 into the liver and the kidney from serum, and as a consequence, decrese the serum TT3 level further.

• Nuclear Engineering and Technology
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• 제7권2호
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• pp.127-133
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• 1975

3,5,3'-Triiodothyronine (T$_3$) 및 Thyroxine(T$_4$)의 요오드 동위원소 교환표지에 어서는 T$_3$:I$_2$ 또는 T$_4$:I$_2$의 몰비, PH, 반응시간 둥이 중요인자 임을 알수 있었다. T$_3$ 및 T$_4$를 평균 45%, 50%로 각각 표지할 수 있는 개량될 표지반응 조건 및 전 반응생성물을 씰리카 젤 박판과 클로로포름, 메탄올, 암모니아 둥을 전개용매로 사용하는 박층크로마토그래피로 신속 간편하게 분리, 정제하는 방법을 제시하였다.

• 약학회지
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• 제27권2호
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• pp.117-124
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• 1983

For development of $EMIT-T_{3}$ assay, the conjugation of 3, 5, 3'-triiodothyroformic acid NHS ester to G6PDH was attempted in various reaction conditions. Up to now, the best conjugation condition was the ratio of $T_{3}$-NHS:G6PDH=100 in 25% carbitol-Tris buffer at pH 9, $0^{\circ}C$ during overnight. The obtained $T_{3}$-G6PDH conjugates usually had 20% residual enzyme activity which was inhibited by 40-70% with various $anti-T_{3}$ antibodies. Utilizing the conjugate I and an antibody (S2633G), a useful standard curve for $T_{3}$ assay was obtained in the range of 0 to 5ng/ml with 499 EMIT units of separation.

• 한국발생생물학회지:발생과생식
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• 제13권4호
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• pp.313-320
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• 2009

본 연구에서는 외인성 T3처리가 강도다리의 담수 순화시 삼투압 조절과 혈중 코티졸 변화에 어떠한 영향을 미치는지 조사하였다. 해수에서 사육 중인 강도다리에 갑상선호르몬의 일종인 T3를 5, 10 및 $15{\mu}g$/g body weight의 농도로 0.9% NaCl과 함께 주입하고 담수로 옮긴 후 3일째 혈액을 채취하였으며, 삼투압 조절 지표인 $Na^+,\;Cl^-$ 및 삼투질 농도와 스트레스 지표인 cortisol, glucose를 조사하였다. $10{\mu}g$/g의 T3를 주입한 실험구의 혈장 $Na^+$와 $Cl^-$의 농도는 호르몬 처리 없이 담수로 이동한 대조구에 비해 유의하게 높았다. 삼투질 농도는 $10{\mu}g$/g과 $15{\mu}g$/g의 농도를 주입한 실험구가 대조구보다 높았다. 그러나 $5{\mu}g$/g 농도의 T3를 주입한 실험구에서는 삼투압 조절에 영향을 미치지 않는 것으로 나타났다. 또한 본 연구에서는 T3를 주입한 모든 실험구에서 glucose와 상관없이 cortisol의 농도가 모두 증가하는 경향을 나타내었다. 이러한 cortisol의 증가는 스트레스 반응이 아닌 삼투압 조절과 관련이 있으며, 결과적으로 강도다리의 담수 순화시 외인 성 T3는 cortisol을 증가시킴으로 어체의 고삼투압 조절 능력을 향상시키는 것으로 추정된다.

• 대한약침학회지
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• 제1권1호
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• pp.53-76
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• 1997

In order to study the effect of aqua-acupuncture with Radix Angelicae gigantis extract solution by acuncture point. Radix Angelicae gigantis aqua-acupuncture was carried on every day for 3 days and 5 days 7 days respectively on the corresponding loci of Chondol(CV22) and non-acupuncture points (loci at the root of tail), after inducing hypothyroidism by thiourea administration and thereafter the contents of serum triiodothyronine$(T_3)$, thyroxine$(T_4)$, triiodothyronine uptake, free $T_3$, free $T_4$, total protein, albumin, creatinine, creatine, BUN, cholesterol, triglyceride, and total lipid were measured in hypothyroidism rats. The following results have been obtained : 1. $T_3$ was increased with statistical significance by Radix Angelicae gigantis aquq-acupuncture for 3 days, 5 days and 7 days Chondol(CV22) and non-acupuncture points as compared with the control group. 2. $T_3$ uptake was increased with statistical significance by Radix Angelicae gigantis aquq-acupuncture for 3 days, 5 days and 7 days to Chondol(CV22) as compared with the control group. 3. Free $T_4$ was increased with statistical significance by Radix angelicae gigantis aqua-acupuncture for 3 days, 5 days and 7 days to Chondol(CV22), and non-acupuncture points respectively as compared with the control group. 4. Albumin was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22), and non-acupuncture points respectively as compared with the control group. 5. Creatine was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22), and non-acupuncture points respectively as compared with the control group. 6. Cholesterol was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22) and non-acupuncture points respectively as compared with the control group. 7. Triglyceride was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22) and non-acupuncture points respectively as compared with the control group. 8. Total lipid was decreased with statistical significance by Radix Angelicae gigantis aqua-acupuncture for 3 days, and 5 days and 7 days to Chondol(CV22) and non-acupuncture points respectively as compared with the control group.

• 한국수산과학회지
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• 제35권6호
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• pp.551-556
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• 2002

출산기에 임박한 조피볼락 암컷에 20mg/kg체중의 농도로 3,5, 3'-triiodo-L-thyronine $(T_{3}$)를 주사한 뒤, 출산 자어로의 호르몬 전이여부, 자어 성장과 활력을 조사하였다. 출산자어의 갑상선 호르몬을 분석한 결과,출산직후에 $T_{3}$구가 대조구 보다 높은 $T_{3}$농도를 나타냈으나, L-thyroxine $(T_{4}$)의 농도는 대조구와 유의차가 없었다. 그러나 실험기간 동안 어체내 모든 실험구의 감상선호르몬 농도는 $T_{3}$가 $T_{4}$에 비해 상대적으로 낮았고, 실험구별로는 $T_{3}$구가 대조구에 비해 높은 것을 알 수 있었다. 자어의 성장률은 대조구에 비하여 T,구에서 높았으며, 출산후 경과일수에 따라 직선적인 전장성장 경향을 나타냈다. 실험종료시 생존율은$ T_{3}$구의 자어가 대조구에 비해 높았으며, 유영활성에서도 $T_{3}$구의 자어가 대조구에 비해 유의하게 높았다. 출산직전 $T_{3}$ 모체주사는 외부영양원 섭취개시기의 자어성장 및 생리활성을 증진시키는 데 효과적인 것으로 나타났다. 이상의 연구결과로부터 출산전 모체주사를 통하여 외인성 $T_{3}$가 자어로 전이되며, 전이된 호르몬은 출산후의 자어의 초기 발달기 동안 생리적으로 긍정적인 작용을 할 것으로 추측된다.

• 대한방사선기술학회지:방사선기술과학
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• 제5권1호
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• pp.55-62
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• 1982

Measurement of triiodothyronine($T_3$) concentration is useful for the diagnosic and treatment of thyroid gland diseases. Fundermental studies of measurement of $T_3$ concentration by radioimmunoassay were performed and values determined by commercially available kit, Coat-A-Count, Diagnostic Products Corporation. The optimal utilization of the radioimmunoassay in measuring $T_3$ concentrations is dependant not only on high quality performance of the assay, but also on their appropriate application to the clinical situation. These are several aspects that must be considered in every individual case. These include factors such as accurate pippeting in reagent preparation in the assay and through decanting to remove all visible moisture after incubation steps and so forth. In attempt to assess quality control of the radioimmunoassay of serum $T_3$, serum pools with high, medium, low $T_3$ concentrations were assayed for each of 5 samples. The results obtained with this study were as follow: 1. The coefficient of variation(C. V.) for the standard curve ranged between $0.2{\sim}3.5%$. 2. It was necessary that both incubation time and temperature should correctly be maintained all the in the assay performance. 3. The precison with the $T_3$ RIA procedure was good. 4. The measured values of serially diluted serum $T_3$ concentration with Ong/dl standard solution was proportional to the predicted values. However dilution curve of distilled water was not strait. 5. Calculated $T_3$ values of patient serum in normal group was $107.8{\pm}25.90ng/dl$ in male patient and $127.29{\pm}24.08ng/dl$ in female patient.

• 대한핵의학회지
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• 제9권1호
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• pp.31-43
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• 1975

In an attempt to establish the diagnostic value of serum triiodothyronine and to correlate it with pathophysiologic mechanisms of thyroid hormones in various thyroid disorders, the author measured the serum triiodothyronine levels by means of radioimmunoassay and compared them with other thyroid function tests. This study was carried out in 152 cases with various thyroid functions; 28 cases as control, 51 cases of hyperthyroidism, 50 cases of euthyroidism and 23 cases of hypothyroidism. The results obtained were as follows: 1. The serum $T_3$ level in normal control group ranged between $131{\pm}34ng/dl$. 2. The serum $T_3$ levels ranged between $306{\pm}97ng/dl$ in hyperthyroidism $138{\pm}32ng/dl$ in euthyroidism and $60{\pm}27ng/dl$ in hypothyroidism. The significant differences between these groups were noted in this study. 3. In 5(9.9%) out of 51 cases with hyperthyoidism and 9(39.1%) of 23 cases with hypothyroidism, the serum $T_3$ were measured to be in normal range. Accordingly, the diagnostic value of the measurement of serum $T_3$ with hyperthyroidism was justifiable, but with hypothyroidism, it was less creditable than that of serum thyrotropin. 4. There was little significant difference between the diagnostic value of serum thyroxine and triiodthyronine levels in various thyroid disorders. However, $T_4/T_3$ ratio was decreased in patients with untreated hyperthyroidism because of more elevation of $T_3\;than\;T_4$. Consequently, the serum $T_3$ was more sensitive than $T_3$ in some thyroid disorders. 5. The serum $T_3$ level was much more sensitive and showed prompt shift in its level during the course of treatment on the patient with various thyroid disorders. And the measurement of serum $T_3$ was a good index for the evaluation of the thyroid function. From these results obtained, the measurement of serum $T_3$ by means of radioimmunoassay is a good way to understand the status of thyroid function with various thyroid disorders and evaluate the effects of the treatment given on these patients.

• 대한스포츠의학회지
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• 제36권4호
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• pp.214-220
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• 2018

Purpose: The purpose of this research is to study changes in pituitary hormone in anterior lobe and thyroid hormone before, after, and during recovery time in severe 100 km ultramarathon. Methods: Healthy middle-aged runners (age, $52.0{\pm}4.8$ years) participated in the test. Grade exercise test is done, and then blood is taken from those participants before and after completing 100 km ultramarathon at the intervals of 24 hours (1 day), 72 hours (3 days), and 120 hours (5 days) to analyze their luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and free thyroxine (Free T4). Results: For LH, it decreased more significantly at 100 km than pre-race. However, after 1 day result increased more than that of 100 km. At 3 days, it was significantly higher than pre-race and 100 km, recovering at 5 days. In terms of FSH, it decreased at 100 km, 1 day, and 3 days more than pre-race but recovered at 5 days. TSH was higher at 1 day and 5 days compared to pre-race. T3 was only higher at 100 km than pre-race. T4 was higher till 5 days at 100 km than pre-race. Free T4 increased more significantly at 100 km than pre-race. Conclusion: In terms of severe long distance running, LH and FSH which belong to hormone from anterior lobe as well as T3, T4, and Free T4 which belong to thyroid hormone showed their variation within the standard range. However, TSH showed abnormal increase from enhanced concentration of blood after marathon becoming hyper-activation even during the recovery period.