• Title/Summary/Keyword: 3% NaCl solution

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Purification of A Novel Protein Induced by Salt Treatment in Rice Seedling (NaCl 처리로 벼 유묘에서 유도된 단백질의 분리와 정제)

  • 민경수;황태익;임현옥;안장순
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.2
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    • pp.134-140
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    • 1997
  • The monoclonal antibody against a novel protein, which was induced in the roots of rice seedlings treated with NaCl, was produced. Oryzea sativa L. cv. Annapruna grains were sown on clean sands and grown for 10days and then the seedlings were soaked in 50mM NaCl aqueous solution. In 48hrs of the NaCl treatment, the roots were collected and homogenated with liquid nitrogen and extraction buffer. The homogenate was centrifuged and to the supernatant 75% ammonium sulfate was added to pre-cipitate proteins. From these proteins a novel protein was purified through DEAE-ion chromatography and FPLC(Phenyl column). This protein appeared as a single band in the native electrophoresis. Using this protein as antigen, monoclonal antibody was produced. Five cell lines that secreted antibodies specifically bound to this protein were constructed.

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Effects of Coagulants and Soaking Solutions of Tofu (Soybean Curd) on Extending its Shelf Life (두부의 저장에 미치는 응고제와 침지액의 효과)

  • Lee, Kap-Sang;Kim, Dong-Han;Baek, Seung-Hwa;Choun, Seung-Ho
    • Korean Journal of Food Science and Technology
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    • v.22 no.2
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    • pp.116-122
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    • 1990
  • In order to improve the shelf life of Tofu, the effect of calcium chloride or acetic acid as coagulants were investigated for microbial and physicochemical changes during the storage in different kinds of soaking solution. The soaking water of Tofu prepared from calcium chloride was found to be spoiled after 15-17 hours of storage at $30^{\circ}C$, by reaching the bacteria count to 10million per ml. The shelf life of the Tofu prepared from acetic acid was better than those prepared by calcium chloride. For the effect of soaking solution on storage life, soaking Tofu in 0.1% acetic acid was found to be more effective than soaking in other solutions of 3% NaCl or 0.1% K-sorbate. The optical density of soaking solution of Tofu increased in proportion to spoilage, and in case of soaked Tofu in 3% sodium chloride and 0.1% K-sorbate solution, it greatly increased. Titratable acidity, and amino nitrogen contents in soaking solution increased as spoilage of Tofu progressed, but pH decreased the early period, one or two days, of stored Tofu and then increased.

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Growth Characteristics and Photosynthesis of Soybean Seedling to NaCl stress in Sand Culture (콩의 유묘기에 있어서 NaCl Stress에 의한 생육특성과 광합성 반응)

  • Cho, Jin-Woong;Kim, Choong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.18 no.4
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    • pp.361-365
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    • 1999
  • This atudy was conducted to determine the growth characteristics and photosynthesis of soybean (Glycine max L. cv. Keumjongkongl) 30 day old seedling to 100mM NaCl concentration containing 1/2 Hoagland`s nutrient solution in sand culture. The nodule formation of root is not found perfectly with NaCl stress. The leaf dry matter weight (g/plant) of stressed plant is more reduction in 77% to control than any other characters. The water content (%) is tend to increase but water potential (MPa) is tend to decrease at NaCl stress. The chlorophyll content (SPAD) is tend to increase at growing leaf age of control but decrease at NaCl stress. The photosynthesis, stomatal conductance and transpiration are tend to decrease sharply at NaCl stress.

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Effect of KNO3 Priming on Various Properties of Kenaf Seed under Non-Saline and Saline Conditions

  • Lee, In-Sok;Kang, Chan-Ho;Lee, Ki-Kwon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.4
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    • pp.373-381
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    • 2017
  • The main objective of this study was to increase the germination percentage of kenaf seeds with less number of times under non-saline and saline conditions. Therefore, the first goal was to assess the response of kenaf seeds to NaCl. The second goal was to evaluate the effects of $KNO_3$ on kenaf seed germination. The germination percentage exhibited a decreasing tendency in germination rate. Plant dry weight was approximately 0.2 g in all treatments at 5 days after germination. As time passed, the electrical conductivity (EC) value of hydro-priming (HP) consistently increased by 8.7 mS/cm at 24 hours of immersion. However, seeds primed with $KNO_3$ showed no difference in EC values even as times passed. Regarding the priming effect, priming in 100 mM $KNO_3$ concentration for 12 hours increased germination up to 85% in $H_20$ solution and in 0 mM $KNO_3$ concentration upto 73.8% under 0.3% NaCl solution, compared to that of Control. Germination synchronization, shoot length, and leaf unfolding of primed seeds were greater than those of the Control. In addition, main root and hair roots appeared more rapidly in the treated seeds and were more abundant compared to that of the Control. The T50 (times to reach 50% of the final germination percentage) of the Control in both $H_20$ and 0.3% NaCl solutions was 18 and 22 hours, respectively. However, when treated $KNO_3$ priming (0 to 100 mM) in $H_20$ and 0.3% NaCl solution, 9 hours was sufficient to reach T50. Primed (hydro-priming and $KNO_3$) seeds had a lower MDG (mean days untill germination; 0.6-0.62) compared to that of the Control (1.13-1.31) in $H_20$ and 0.3% NaCl solutions. Regarding dry weight of plants after priming, an increasing tendency after the priming treatment in the H20 solution was observed. Furthermore, no significant difference in plant dry weight under 0.3% NaCl stress was observed between the Control and primed seeds. Taken together, the results suggest that 50-100 mM $KNO_3$ priming for 24 hours optimize seed germination rate in less number of times of exposure with great vigor. Therefore, it is recommended for kenaf seed invigoration before planting.

CHEMICAL DEGRADATION OF LIGHT CURED COMPOSITE RESINS IN NAOH SOLUTION (NaOH 용액내에서의 광중합형 복합레진의 화학적 분해)

  • Kim, Jung-Ran;Jeong, Byung-Cho;Yang, Kyu-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.3
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    • pp.469-477
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    • 2002
  • One of the major deficiencies of composite restorative resins is their insufficient resistance to wear. Of the multitude of factors that have been associated with wear, subsurface degradation within the restoration is considered to be one. The aim of this study was to evaluate the resistance to degradation of four commercial composite resins in an alkaline solution. The brands studied were Z100(3M), Clearfil AP-X(Kuraray), Tetric Ceram(Vivadent), Aelit flo(Bisco). Resistance to degradation was evaluated on the basis of the following parameters: (a) mass loss(%) - determined from pre-and post-exposure specimen weights: (b) Si loss(ppm) - obtained from ICP-AE analysis of solution exposed to specimens; and (c) degradation depth(${\mu}m$) - measured microscopically (SEM) from polished circular sections of exposed specimens. The results were as follows: 1. The sequence of the mass loss was in ascending order by AE, EL, TC, Z100. There was statistically significant difference of mass loss between AE, CL group and TC, Z100 group(p<0.05). 2. The sequence of the degree of degradation layer depth was in ascending order by AE, CL, TC, Z100. But there was no statistically significant difference of degree of degradation layer depth between AE and CL(p<0.05). 3. For the Si concentration, Z100 was the highest of all. 4. The correlation coefficient between mass loss and degradation depth was relatively high(r=0.71 p<0.05).

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Study on the Salt Tolerance of the Several Forage Crops (몇가지 사료작물(飼料作物)의 내염성에(耐鹽性) 관(關)한 연구(硏究))

  • Kim, Choong Soo;Kim, Yang Sik
    • Korean Journal of Agricultural Science
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    • v.11 no.2
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    • pp.183-189
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    • 1984
  • This study was carried out to investigate the salt tolerance to the seed germination of 4 forage crops and their critical salt concentration. 1. The rate of seed germinations of 4 forage crops at 0.4% NaCl solution was not significantly decreased compared with control plot but the germination percentages at 0.8% NaCl solution were significantly dropped. 2. The days for germination were gradually delayed by increasing salt concentrations. 3. The growth of seedling root and shoot was slightly depressed at 0.4% salt solution but significantly depressed at 0.8% solution. At 1.2% salt solution, the seedling growth was stopped immediatly after seed germination. 4. The NaCl content in the seedling was increased at the medium containing higher amount of NaCl, however increasing rate of NaCl level in the seedling showed significant differences among the forage crops tested in this experiment. 5. The critical salt concentration to the germination of the forage crops was 0.4% to 0.8%. The perennial ryegrass showed the highest salt tolerance in the seed germination, and the tall fescue and the creeping red fescue showed medium, and the orchardgrass showed the lowest tolerance.

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Uncertainty evaluation in electrochemical noise resistance measurement (전기화학적 노이즈 저항 측정에서의 불확도 평가)

  • Kim, Jong Jip;Kang, Su Yeon
    • Corrosion Science and Technology
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    • v.12 no.5
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    • pp.220-226
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    • 2013
  • The uncertainty in statistical noise resistance measurement was evaluated for a type 316 stainless steel in NaCl solutions at room temperature. Sensitivity coefficients were determined for measurands or variables such as NaCl concentration, pH, solution temperature, surface roughness, inert gas flow rate and bias potential amplitude. The coefficients were larger for the variables such as NaCl concentration, pH, inert gas flow rate and solution temperature, and they were the major factors increasing the combined standard uncertainty of noise resistance. However, the contribution to the uncertainty in noise resistance measurement from the above variables was remarkably low compared to that from repeated measurements of noise resistance, and thus, it is difficult to lower the uncertainty in noise resistance measurement significantly by lowering the uncertainties related with NaCl concentration, pH, inert gas flow rate and solution temperature. In addition, the uncertainty in noise resistance measurement was high amounting to 17.3 % of the mean, indicating that the reliability in measurement of noise resistance is low.

Substitutional Adsorption and Thermodynamic Characteristics of Proton and Di-iso-butylnitrosoamine in NaCl Aqueous Solution (NaCl 수용액내의 양성자와 디이소부틸니트로소아민의 치환흡착과 열역학적 특성)

  • HWANG Kum-Sho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.16 no.3
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    • pp.239-245
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    • 1983
  • Electrochemical adsorption always was accompanied with solvent displacement and relative size factor(x) of adsorbate and solvent and hydrogen coverage(${\theta}$) on the lead anodic film electrode formed in phosphoric acid in NaCl solution and the sea water at $15{\sim}35^{\circ}C$ were studied by means of constant current-potential method and potentiodynamic cathodic polarization method. In this experiment, various constants and thermodynamic quantities calculated from the hydrogen coverage were also described to explain the reactivities of di-iso-butylnitrosoamine(DBNA) and proton ($H^+$) according to the changes of interactions between solute and solvent in the bulk phase and interphase. It was investigated that the average values of relative size factor and the coverage of hydrogen atoms studied with the electrode of lead anodic film formed in phosphoric acid solution in 60mM DBNA+0.5M NaCl and in 60mM DBNA+$6\%0$ sea water were about 11.0 and 0.2 respectively. Hydrogen evolution was electrochemical mechanism because of substitutional adsorption of aromatic substance with their delocalization of electrons, but in the case of non-charge transfer adsorption of aliphatic substance(DBNA) interacting relatively little with the electrode, it was combination mechanism.

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Characteristic of Oxidants Production and Dye Degradation with Operation Parameters of Electrochemical Process (전기화학적 공정의 운전인자에 따른 산화제 생성과 염료 분해 특성)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.18 no.11
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    • pp.1235-1245
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    • 2009
  • The purpose of this study is to investigate electro-generation of free Cl, $ClO_2$, $H_2O_2$ and $O_3$ and degradation of Rhodamine B in solution using Ru-Sn-Sb electrode. Electrolysis was performed in one-compartment reactor using a dimensionally stable anode(DSA) of Ru-Sn-Sb/Ti as the working electrode. The effect of applied current (0.5-3 A), electrolyte type (NaCl, KCl, HCl, $Na_2SO_4$ and $H_2SO_4$) and concentration (0.5-2.5 g/L), air flow rate (0-3 L/min) and solution pH (3-11) was evaluated. Experimental results showed that concentration of 4 oxidants was increased with increase of applied current, however optimum current for RhB degradation was 2 A. The generated oxidant concentration and RhB degradation of the of Cl type-electrolyte was higher than that of the sulfate type. The oxidant concentration was increased with increase of NaCl concentration and optimum NaCl dosage for RhB degradation was 1.75 g/L. Optimum air flow rate for the oxidants generation and RhB degradation was 2 L/min. $ClO_2$ and $H_2O_2$ generation was decreased with the increase of pH, whereas free Cl and $O_3$ was not affected by pH. RhB degradation was increase with the pH decrease.

Effect of Synthetic Hydrotalcite on Salt Water Resistance of Chloroprene rubber Foam (Synthetic Hydrotalcite가 클로로프렌 고무 발포체의 내염수성에 미치는 영향 연구)

  • Park, Eun Young;Seo, Eun Ho;Lim, Sung Wook
    • Textile Coloration and Finishing
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    • v.31 no.3
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    • pp.177-186
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    • 2019
  • In this study, we investigated for synthetic hydrotalcite in chloroprene rubber foam. Experiments were carried out to find the optimum content ratio by controlling the contents of MgO and Hydrotalcite. Swelling test in toluene immersion was made to measure the crosslinking density of CR foams, and the cure properties were investigated with flat die rheometer and Mooney viscosity. The difference of hardness, tensile strength and elongation at break were observed after immersing in 7% NaCl or 21% NaCl solutions for a day and four days. In addition, the volume change and water content remaining in CR foam were measured after immersing NaCl solution. As content of MgO increased, the value of the cure torque tended to increase, but it was almost constant above 2phr of MgO. However, the Mooney viscosity decreased with increasing MgO content. The crosslinking density, determined by the swelling ratio, showed that the CR compound without MgO showed a higher degree of swelling. When the content of hydrotalcite/MgO was 3:2, it was the lowest volume change of CR form. Also, As the content of hydrotalcite decreased, the difference of mechanical properties before and after immersion NaCl solution increased.