• Applied Biological Chemistry
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• 제36권2호
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• pp.121-126
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• 1993

참깨박에 함유되어 있는 불용성 형태의 단백질을 가용성 형태의 단백질로 용출시키기 위하여 Bacillus sp. CW-1121이 생성하는 효소를 작용시켰다. 이때 참깨박의 단백질 용출을 위한 최적 pH는 7.5였으며, 최적 온도는 $40^{\circ}C$였고 최적 조건하에서 2시간의 소화로 약 60%의 용해도를 보였다. 단백질의 용해도는 참깨 단백질이 pH4.5에서 가장 낮아 등전점을 보였고, pH3.0과 pH6.0 이상에서는 크게 증가하였다. 효소처리 참깨박 단백질을 전기영동한 결과 수용성 단백질은 4개, 염용성 단백질은 2개의 밴드가 관찰되었다. 분리 단백의 아미노산 조성은 수용성 단백질의 경우 serine이 17.24 mg/g, 염용성 단백질은 glutamic acid가 10.77 mg/g, 유리아미노산은 glutamic acid가 6.55 mg/g으로 가장 많이 함유되어 있었으며 특히 필수아미노산의 조성도 상당히 좋은 편이었다.

• 한국간호교육학회지
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• 제24권3호
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• pp.214-224
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• 2018

Purpose: This study examines the effectiveness of an educational program of nursing biomedical ethics for nursing students to increase awareness of the Code of Ethics for Nurses, moral sensitivity, and application of the Code of Ethics for Nurses. Methods: A quasi-experimental research design and t-test were used. The experimental group (n=31) and control group (n=32) were second-year university nursing students in two major cities. The experimental group received 15 hours of nursing ethics education during the 15-week semester. The training was not provided to the control group. Results: The application of the Code of Ethics for Nurses increased significantly in the experimental group (t=-1.06, p=.017), nurses and patient (t=-2.23, p=.029), obligation of nurse as an expert (t=-2.08, p=.042), nurse and cooperator (t=-2.54, p=.014). The consciousness of biomedical ethics increased significantly in the experimental group (t=4.28, p=.021), newborn's right to live (t=-2.61, p=.011), euthanasia (t=-2.36, p=.021). Conclusion: The results of the study show that providing a nursing biomedical ethics program to nursing students is an effective method to enhance the application of the Code of Ethics for Nurses and Consciousness of Biomedical Ethics. Implementing an intervention program of the Code of Ethics for Nurses in the regular nursing curriculum may reduce conflicts involving ethical decision making by nurses.

• 성인간호학회지
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• 제24권2호
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• pp.139-148
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• 2012

Purpose: A study was designed to compare heart rate variability (HVR) of older adults with postprandial hypotension (PPH) with older adults with no PPH. Methods: The samples were community-dwelling older adults (${\geq}$ 60 years old) with PPH (n=35) and without PPH (n=35). HRV was measured by 10 minutes of electrocardiography using Physiolab 400 and analyzed in time domain (SDNN) and with power spectral analysis in frequency domain (TP, LF, HF, LF/HF). Blood pressure was measured every 15 minutes before and until 120 minutes after the meal. Results: All HRV parameters were lower in the PPH group compared to those with no PPH. Specifically the measures of SDNN, TP, LF, and LF/HF of the PPH group were significantly lower (U=385.0, $p$=.008; U=361.0, $p$=.003; U=375.0, $p$=.005; U=368.0, $p$=.004, respectively). Further, the LF and LF/HF of SBP (${\geq}140$ mmHg) group and the LF/HF of DBP (${\geq}$ 90 mmHg) group were significantly lower ($x^2$=6.33, $p$=.042; $x^2$=33.10, $p$ <.001; $x^2$=11.03, $p$=.004, respectively). Conclusion: These findings indicate that the autonomic nervous functions in persons with PPH are less activated than those persons with no PPH. The findings contribute to an understanding of HRV and the association of autonomic nervous function with the mechanism of PPH.

• Archives of Pharmacal Research
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• 제24권6호
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• pp.568-571
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• 2001

The purpose of this work was to study the effect of storage time and temperature on the in vitro release kinetics of a commercial sustained-release dosage form of theophylline, at different pHs of the dissolution medium. The formulation was stored at $35^{\circ}C$ for 16 months and at $45^{\circ}C$ for 8 months, with a relative humidity of 60%. The in vitro release tests were performed at pHs 2, 4, 6 and 7.4. The mean values of the transport coefficient n, were close to 0.5 in all the conditions tested, which indicates that the transport system is not modified after storage of the formulation at $35^{\circ}C$ and $45^{\circ}C$. The mean values of the dissolution rate constant ranged from 0.036 to 0.043 $min^{-n}$, under all the conditions tested. Significant differences (${\alpha}=0.05$) were found between pHs 2, 4 and 6, 7.4 for all the model-independent parameters studied. When the formulation was kept at $35^{\circ}C$ for 16 months, the mean percentage of drug dissolved at 8 hours was 25.61% (pHs 2, 4) and, 36.12% (pHs 6, 7.4), representing a 26% and 24% reduction, respectively. Simitar results were obtained after storing the formulation at $45^{\circ}C$ for 8 months, corresponding to 33.3% (pHs 2, 4) and, 22.5% (pHs 6, 7.4) diminution, respectively. The values of the similarity factory $f_2$, obtained were lower than 50, which indicates the lack of similarity among the dissolution profiles, after storing the formulation under the experimental Conditions tested.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.952-956
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• 2011

The objective of the study was to evaluate the effect of passive transfer status, determined by measuring serum immunoglobulin (Ig) concentration 24 hours after parturition, on growth performance in buffalo calves allowed to nurse the dam during the first month of life. Serum Ig concentration 24 hours after birth ranged from 28.1 to 35.9 mg/ml, birth weight ranged from 29 to 41 kg, body weight 30 days after birth ranged from 48.5 to 62.9 kg. The Average Daily Gain (ADG) from birth to day 30 ranged from 448 to 1,089 g/d. Significant linear associations were detected between serum Ig concentration 24 hours after birth and day-30 weight (p< 0.05; $R^2$ = 0.31) and between serum Ig concentration 24 hours after birth and ADG from birth to day 30 (p<0.001; $R^2$ = 0.72). Results indicated that passive transfer status was a significant source of variation in growth performance when buffalo calves nursed the dam. Maximizing passive transfer of immunity by allowing calves to nurse the dam can increase growth performance during the first month of life.

• The Korean Journal of Physiology and Pharmacology
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• 제1권3호
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• pp.263-273
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• 1997

The purpose of this study was to evaluate the effects of electrical stimulation on vestibular compensation following ULX in rats. Electrical stimulation (ES) with square pulse ($100{\sim}300uA$, 1.0 ms, 100 Hz) was applied to ampullary portion bilaterally for 6 and 24 hours in rats receiving ULX. After ES, animals that showed the recovery of vestibular symptoms by counting and comparing the number of spontaneous nystagmus were selected for recording resting activity of type I, II neurons in the medial vestibular nuclei (MVN) of the lesioned side. And then the dynamic neuronal activities were recorded during sinusoidal rotation at a frequency of 0.1 Hz and 0.2 Hz. The number of spontaneous nystagmus was significantly different 24 hours (p<0.01, n=10), but not 6 hours after ULX+ES. As reported by others, the great reduction of resting activity only in the type I neurons ipsilateral to lesioned side was observed 6, 24 hours after ULX compared to that of intact labyrinthine animal. However, the significant elevation (p<0.01) of type I and reduction (p<0.01) of type II neuronal activity were seen 24 hours after ULX+ES. Interestingly, gain, expressed as maximum neuronal activity(spikes/sec)/maximum rotational velocity(deg/sec), was increased in type I cells and decreased in type II cells 24 hours after ULX+ES in response to sinusoidal rotation at frequencies of both 0.1 Hz and 0.2 Hz. This result suggests that accompanying the behavioral recovery, the electrical stimulation after ULX has beneficial effects on vestibular compensation, especially static symptoms (spontaneous nystagmus), by enhancing resting activity of type I neurons and reducing that of type II neurons.

• 한국수정란이식학회지
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• 제27권4호
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• pp.253-258
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• 2012

This study was carried out to effects of ethylene glycol concentration, sucrose and culture day of in vitro production embryo on slow-down freezing in Hanwoo. 6, 7, 8 and 9 day embryos produced in vitro were frozen using 1.8M EG+0.1M sucrose, 1.8M EG+0.5% BSA and 1.5M EG+0.1M sucrose media. Survivability was confirmed after frozen-thawed 24 and 48h and ICM, TE cell number were counted by Hoechst 33342 and PI staining after frozen-thawed 24h. As a result, 1.8M EG+0.1M sucrose group was most significantly (p<0.05) higher compared with the other treatment groups on survivability, TE and total cell number after frozen-thawed 24h ($94.2{\pm}2.6%$, $94.67{\pm}3.4$ and $129.67{\pm}5.5$). ICM number did not found significant (p<0.05) differences between the three treatment groups. in 6, 7, 8 and 9 day of embryos using three types of freezing media, frozen-thawed, 1.8M EG+0.1M sucrose groups with embryos cultured 8 day was significantly (p<0.05) highest survivability to $98.3{\pm}1.7%$ after frozen-thawed 24h. 1.5M EG+0.1 sucrose group with embryos cultured 9 day was significantly higher survivability than group of embryos cultured 8 day after frozen-thawed 24 and 48h. In conclusion, 1.8M EG+0.1M sucrose media is considered to be effective to cryopreservation of embryos cultured 8 and 9 day.

• 대한물리의학회지
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• 제5권2호
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• pp.211-221
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• 2010

Purpose : The purpose of this study is to find out the effects of combined exercise including walking and yoga on cognitive functions in the elderly women. Methods : Sixteen elderly women aged above 65 are invited in this study. Each subject participated in exercise three times a week for eight weeks from July 14th to September 13th in 2008. The changes between pre and post exercise are analyzed by Wilcoxon sign rank test and repeated ANOVA test with SPSS (ver 17.0) package program. Results : After exercise, In the below 23 points group, only interference STROOP test (p<.05) among sub items of Cognition Scale for Older Adults (CSOA) is improved significantly. In the above 24 points group, words memory (p<.05), delayed recall (p<.05), and picture naming (p<.05) among sub items of CSOA are improved significantly. But it is no different to the change patterns among two groups. Conclusion : These results show that combined exercise including walking and yoga is helpful to improve cognitive functions. And we find that exercise is helpful in the above 24 points elderly women more than in the below 23 points.

• Journal of Korean Biological Nursing Science
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• 제24권3호
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• pp.190-199
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• 2022

Purpose: This study investigated the effect of pressure injury nursing knowledge, and pressure injury nursing attitudes, on pressure injury nursing practice. Methods: Participants in this descriptive study were 141 nurses at 20 geriatric hospitals. Data was collected August 24, 2021- April 4, 2022, and analyzed in terms of Independent t-test and one-way ANOVA, Scheffé test, Pearson's correlation coefficient, and multiple regression, using the SPSS/WIN 27.0 program. Results: Pressure injury nursing practice positively correlated with pressure injury nursing attitudes (r= .44, p< .001). Factors influencing pressure injury nursing practice were pressure injury nursing attitudes (β= .43, p< .001), and gender (β= .21, p= .006). The model used in this study explains 22.8% of pressure injury nursing practice (Adjusted R²= 22.8, F= 11.30, p< .001). Conclusion: Results show factors that influence pressure injury nursing practice of nurses in geriatric hospitals. Based on the results, pressure injury nursing practice programs must include factors that improve pressure injury nursing attitudes. A follow-up study to confirm the impact of developing a program for increasing pressure injury nursing practice is also recommended.

• 디지털융복합연구
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• 제15권9호
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• pp.249-259
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• 2017

본 연구의 목적은 발-반사 마사지가 지역사회 재가노인의 수면, 우울 및 변비에 미치는 효과를 검증하는 것이다. 본 연구는 비동등성 대조군 전후설계 유사실험연구이다. 연구대상자는 D광역시 소재 2개 노인복지관을 이용하는 65세 이상 노인 46명이다(실험군=24명, 대조군=22명). 실험군은 2주간 1회 20분씩 주 5회, 총 10회의 발-반사 마사지에 참여하였다. 수집된 자료는 SPSS 22.0 프로그램을 이용하여 서술통계, ${\chi}^2-test$, independent t-test, Pearson correlation coefficient로 분석하였다. 본 연구결과, 2주 후 발-반사 마사지에 참여한 실험군은 참여하지 않은 대조군에 비해 통계적으로 유의한 수면증진 (t=-5.60, p<.001), 우울감소(t=3.93, p<.001), 및 변비완화 (t=4.90, p<.001)효과가 나타났다. 따라서 발-반사 마사지는 재가노인의 수면증진과 우울 및 변비 감소에 효과적인 중재임이 확인되었다.