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Effects of Fermented Products by Formitella fraxinea and Sarcodon aspratus on In Vitro Ruminal Fermentation (In vitro 반추위 발효에 미치는 Formitella fraxinea와 Sarcodon aspratus 발효물질의 영향)

  • Kim, Yong-Kook
    • Korean Journal of Agricultural Science
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    • 제31권1호
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    • pp.15-25
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    • 2004
  • In order to determine the effect of fermentation by the mycelia of fungal species, Formitella fraxinea and Sarcodon aspratus, on the in vitro dry matter digestibility and pH of mixtures with sawdust plus 20% wheat bran w/w, on dry matter basis to use as a feedstuff or an additive including fungal mycelium into a feedstuff. The mixtures were unfermented (UF) and fermented by Formitella fraxinea(FF) and Sarcodon aspratus(SA) for two weeks at $29^{\circ}C$ in a incubator. Fungal fermentation products were added to the basal diet to the level of 0, 1, 3 and 5%, w/w of diets each. The in vitro dry matter digestibilities, soluble sugar contents and pH of fermentation fluids were measured at 24, 48 and 72hr after fermentation begin. Neutral detergent fiber(NDF) contents in mixtures were lower for SA and UF(80.4 and 82.2%) than for FF(88.3%) (P<0.05). In vitro DM digestibility for 48h was higer for SA(21.2%) than for UF and FF(17.9 and 12.2%). The in vitro dry matter digestibility for 24hr was higher for diets added with FF 1% as 49.18%, and lower for diet added with FF 5%(43.07%) than basal diet(44.98%)(P<0.05), and tended to be higher for the diets added with fungal products. The pH of in vitro fermentation fluids for 24 and 48 hrs fermentation were lower for diets added with all FF and SA than for UF(P<0.05). However, those for 72 hrs fermentation were higher for SA 1%(6.74) than other diets(P<0.05). The soluble sugar concentration of in vitro fermentation fluid was not different between diets for 24 hr fermentation. However, those were higher for all additive diets than basal diet for 48 and 72 hrs fermentation(P<0.05). It could be concluded that dairy cow's diets added with fungal fermentation products have positive effects, and expected it will be more beneficial if more fungal mycelium was contained.

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Feed Value and Fermentation Quality of Covered Barley Grain Silage with respect to Days after Heading in Honam Region of Korea (호남지역 수확시기에 따른 겉보리 곡실발효사료의 사료가치 및 발효품질)

  • Park, Jong-Ho;Oh, Young-Jin;Cheong, Young-Keun;Song, Tae-Hwa;Park, Tae-Il;Lee, Kwang-Won;Kim, Kyong-Ho;Kim, Yang- Kil;Park, Jong-Chul;Kim, Bo-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • 제62권1호
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    • pp.16-23
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    • 2017
  • This study aimed to determine the optimal harvesting time for covered barley to make grain silage, in Honam region of Korea. We harvested six varieties of barley every third day from 24 to 42 days after heading (DAH). The moisture content decreased from 62.4% at 24 DAH to 24% at 42 DAH. The moisture content at 36 DAH was 44.3%; however, moisture content at 39 and 42 DAH was lower than 40%. Yield of covered barley significantly increased from 24 to 42 DAH (p < 0.05). Yield at 36 DAH (557 kg/10a) was not significantly different from that at 39 and 42 DAH (p < 0.05). With respect to the feed value of barley grain silage, the amount of crude fiber and crude ash was different by harvesting time (p < 0.05). However, the amount of crude protein, crude fat, and total digestible nutrients (TDN) from 24 and 42 DAH was not significantly different. The pH of grain silage from 24 to 42 DAH was between 3.8 and 4.2 and it was stable until 36 DAH (p < 0.05). However, the pH of grain silage at 39 and 42 DAH was 5.2 and 5.8, respectively, which was higher than the pH of silage with good fermentation quality. The lactic acid content of barley grain silage from 24 to 42 DAH decreased from 5.5% to 0.5% (p < 0.05). The amount of lactic acid at 36 DAH was higher than that at 39 and 42 DAH (p < 0.05). With respect to moisture content, yield, feed value, and fermentation, the optimal harvesting time for grain silage of covered barley was 36 DAH. This could increase the use efficiency of harvesting machine for barley and reduce the harvesting time gap between whole barley silage and grain silage in Korea. Moreover, using barley grain silage for animal feed could reduce the import of corn.

Hydration and pH of the Stratum Corneum in High-risk Newborns in the First 2 Weeks of Life

  • Ahn, Young Mee;Sohn, Min;Lee, Sangmi
    • Child Health Nursing Research
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    • 제24권3호
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    • pp.345-352
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    • 2018
  • Purpose: The study was conducted to measure stratum corneum hydration (SCH) and pH (SCP) in high-risk newborns in the early postnatal period and to explore the features related to patterns of change in those parameters. Methods: SCH and SCP were measured on the dorsal hand in 99 hospitalized newborns during the first 14 days of life and the results were analyzed using a general linear model. Results: The mean hydration was 42.9% on day 1, which decreased to 34.6% by 2 weeks (F=15.61, p=<.001). An association was observed between SCH and prematurity (F=21.12, p<.001), as well as for their interaction (F=8.11, p<.001). The mean SCP was $6.2{\pm}0.3$ on day 1, and decreased to $5.7{\pm}0.2$ (F=95.75, p<.001), with no association with prematurity. After adjusting for birth weight, SCH was higher in newborns with vaginal delivery (F=9.07, p=.023) and who received phototherapy (F=11.81, p=.011). For SCP, only delivery type had a significant influence (F=6.40, p=.044). Conclusion: This study suggests that SCH is typically in the 30% range during the early postnatal period, and that an acid mantle on the SC surface is very unlikely to form; these findings could be applied to the nursing process for promoting skin integrity in high-risk neonates.

Real Effect of pH on CIE L*, a*, and b*, of Loins during 24 h Chilling of Beef Carcasses

  • Min, J.S.;Kim, I.S.;Yoon, Y.T.;Lee, M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권2호
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    • pp.279-282
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    • 2002
  • Twenty six male Hanwoo (Korean cattle) carcasses were measured for pH, temperature and instrumental color changes of loins during 24 h post-mortem carcass chilling at $4^{\circ}C$ in the cooler. The average internal temperature of loins was about $5^{\circ}C$ after 24 h of chilling, and with the exclusion of those with an ultimate pH>6.0 (dark-cutters), the average pH value was 5.5. When all carcasses were considered for the partial correlation coefficient between color and pH, with the temperature effect excluded, CIE $L^*$, $a^*$ and $b^*$ seemed to be affected significantly by pH during chilling process (p<0.001). However, when carcasses with dark-cutting condition were excluded, the correlation coefficients were much lower. In contrast, when the partial correlation coefficients between color and temperature, excluding the effect of pH on them, were analyzed, the relationship between color and temperature did not change much after values of DFD (dark, firm, dry) beef were excluded. The results suggested that the known interrelationship of color and pH in chilled beef loins be mainly due to the influence of temperature on pH and color.

Effect of Brazilin from Caesalpinia sappan L. on the Growth of Streptococcus mutans ATCC 25175 (소목으로부터 분리된 Brazilin이 Streptococcus mutans ATCC 25175의 생장에 미치는 효과)

  • Kwon, Hyun-Jung;Han, Man-Deuk
    • Journal of dental hygiene science
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    • 제12권3호
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    • pp.209-215
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    • 2012
  • Somok, the heart wood of Caesalpinia sappan is used in traditional Chinese medicine. This study was performed to investigate the effect of growth and culture conditions of brazilin from C. sappan against S. mutans ATCC 25175. The bacteria were cultured in brain heart infusion (BHI) broth, and then incubated under 5% $CO_2$ at $37^{\circ}C$ for 18-24 hours. The effect of brazilin against S. mutans was confirmed under the changes of the culture conditions, such as growth curve and the change of pH, protein, and total carbohydrate. The growth of S. mutans in control medium was the highest at 24 hr, while brazilin-added medium (0.3 mg/ml) showed maximum growth at 32 hr. The pH values of the control medium was 5.25 at 16 hr, but the media supplemented with brazilin (0.3 mg/ml) was 7.0 at 16 hr. The amounts of total carbohydrate of the control medium was 11 mg/ml at 8 hr, but the brazilin-added media (0.3 mg/ml) was 18 mg/ml at 8 hr. In the protein change of the culture medium, the control culture broth and the brazilin supplemented-cultures was 2.4 mg/ml and 2.54 mg/ml at 24 hr, respectively. Polysaccharide contents of the control medium and test media were 3 mg/ml and 2 mg/ml at 8 hr, respectively. Thus, the application of C. sappan can be considered a useful and practical material for the prevention of dental caries.

Effect of Photoperiod Considering Animal Welfare for Growth Ability and Blood Properties in Broiler Chicks (동물복지를 고려한 점등시간이 육계 병아리의 성장 능력 및 혈액 성상에 미치는 영향)

  • Kim, Hee-Jin;Son, Jiseon;Jeon, Jin-Joo;Kim, Hyun-Soo;Kang, Hwan-Ku;Lee, Woo-Do;Yun, Yeon-Seo;Hong, Eui-Chul
    • Korean Journal of Poultry Science
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    • 제49권3호
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    • pp.139-144
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    • 2022
  • This study was conducted to investigate the effect of three different photoperiods on growth performance, blood properties, and stress indicators in broiler chicks between 1-7 days after hatching. Two hundred and fifty-two 1-day-old male broiler chicks (57.0±0.12 g) were divided into three treatments, with 4 replicates per treatment and 22 birds per replicate subjected to three different photoperiods of 24L, 22L/2D and 18L/6D. A light-emitting diode bulb served as the light source, with an illuminance of 30 lx. As an experimental diet, a commercial feed based on a corn-soybean meal, with 22% CP and 3,150 kcal/kg ME diet, and water were fed ad libitum. Body weight gain, feed conversion ratio, and liver weight ratio showed a statistically significant difference between the 18L/6D and 24L treatments (P<0.05), but with no significant difference between the 22L/2D treatment and either the 24L or 18L/6D treatment. The breast meat ratio was 5.59% in the 18L/6D treatment group, which was lower than that of other treatment groups (P<0.05). The triglyceride levels were highest (P<0.05) in the 18L/6D treatment among treatments, but alanine aminotransferase levels were significantly higher (P<0.05) in the 22L/2D treatment than in the 24L treatment. Levels of cytokines, i.e., Interleukin-6 and Tumor Necrosis Factor-α did not show a significant difference among the treatments, but corticosterone content was significantly higher (P<0.05) in the 24L treatment than in the 18L/6D treatment. In conclusion, 22 hours of lighting is appropriate between 1~7 days after hatching, considering growth performance and the overall health of broiler chicks.

Factors Influencing Unmarried Mothers' Parenting Stress: Based on Depression, Social Support, and Health Perception (미혼모의 양육 스트레스 영향요인: 우울, 사회적 지지, 건강지각을 중심으로)

  • Oh, Eun Jeong;Kim, Hye Young
    • Women's Health Nursing
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    • 제24권2호
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    • pp.116-125
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    • 2018
  • Purpose: To identify factors affecting parenting stress of unmarried mothers. Methods: The subjects were 108 unmarried women who were parenting their children under the age of six. The data was collected from December 15, 2016 to March 5, 2017. The instruments consisted of self-reported questionnaires that included93 items: 21 on depression, 16 on social support, 20 on health perception, and 36 on parenting stress. The collected data were analyzed by t-test, ANOVA, Pearson correlations, and hierarchical multiple regression. Results: The factors influencing parenting stress were health perception (${\beta}=-.55$, p<.001), evaluative support (${\beta}=-.42$, p=.005), informative support (${\beta}=-.35$, p=.040), emotional support (${\beta}=-.24$, p=.045), partner support (${\beta}=-.20$, p=.048), and depression (${\beta}=.14$, p=.033), and the explanatory power was 57%. Conclusion: In order to alleviate the parenting stress of unmarried mothers, it is necessary to develop programs considering the physical, psychological, and social factors of unmarried mothers. Their subjective assessment of their health can increase or decrease their parenting stress, so it is necessary to develop parenting-stress intervention focusing on health perception.

H.P.L. Value in Serum of Normal Pregnancy and Pospartum State by Hemagglutination-Inhibition Reaction (정상(正常) 임산부(妊産婦)의 혈청중(血淸中) H.P.L.의 면역학적(免疫學的) 측정(測定)에 관(關)한 연구(硏究))

  • Chung, Ae-Rhee;Shin, Myun-Woo
    • Clinical and Experimental Reproductive Medicine
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    • 제3권1호
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    • pp.13-19
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    • 1976
  • Serum levels of human placental lactogen have been measured by hemagglutination-inhibition reaction in 67 normal pregnant state and in 15 postpartum 24 hour state, HAIR is less sensitive and reliable method than radioimmunoassay, but simple, rapid, less expensive and fairly accurate, so it is more helpful in screening of large antenatal population with or without high risk complications. 1) Sensitivity of HPL-HAIR test kit was $0.1{\mu}g$/ml of H.P.L. serum level and had no cross reaction to HCG or male serum or non-pregenant female or newborn infant, 2) H.P.L. value was around $2{\mu}g$/ml until 24th week of pregnancy and rose to $6{\sim}8$ ${\mu}g$/ml continuously until about 36th week of pregnancy and then slightly decreased or stationary. 3) H.P.L. value in postpartum 24 hour state was undetectable. 4) There was poor correlation between maternal serum H.P.L. value at term and baby weight.

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Change of the Moisture and Temperature in Planting Ground as Effected by Different Soil Thickness, Soil Mixture Ratios and Ground Cover Plants in the Green Roof System (옥상녹화에서 토심, 토양배합비 및 지피식물에 따른 식재지반 수분 및 온도변화)

  • Ju, Jin Hee;Yoon, Young Han
    • KIEAE Journal
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    • 제10권3호
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    • pp.11-16
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    • 2010
  • This paper has attempted to investigate the change in soil moisture volume and temperature of architecture by planting ground(soil thickness and soil mixture ratio) and ground cover plants(Sedum sarmentosum, Zoysia japonica, Chrysanthemum zawadskii) for middle region green roof system. For this, a test was conducted on the roof of Konkuk University building from April 2009 to October 2009. In terms of treatment, five types(SL, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$, $P_4P_4L_2$) depending on soil mixture ratio and two types(15cm, 25cm) by soil depth were created. Results of soil moisture volume by soil mixture ratio in the 15cm soil thickness showed that the difference was significance between simple soil and mixture soil treatment, however, the statistical significance was not recognized according to soil mixture ratio. In case of 25cm soil thickness, soil moisture volume by soil mixture ratio was more higher 7Vol.%~10Vol.% in the mixture soil than simple soil treatment. In terms of districts planted ground cover plants, soil volume moisture differed among plants in the order Zoysia japonica 17.74 Vol.%$34.86^{\circ}C$, district non-planted $27.49^{\circ}C$, Sedum sarmentosum $25.11^{\circ}C$, Chrysanthemum zawadskii $23.08^{\circ}C$, Zoysia japonica $24.45^{\circ}C$ respectively So, concrete surface showed more higher $5^{\circ}C{\sim}15^{\circ}C$ than other things among the all the time. Result of inner temperature of the architecture and soil, it was measured inner of architecture $25.69^{\circ}C$, inner district non-planted $24.29^{\circ}C$, Chrysanthemum zawadskii $23.90^{\circ}C$, Zoysia japonica $24.02^{\circ}C$, Sedum sarmentosum $25.13^{\circ}C$, respectively.

Effect of Choice Feeding on the Nutrient Intake and Performance of Broiler Ducks

  • Men, Bui Xuan;Ogle, Brian;Lindberg, Jan Erik
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권12호
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    • pp.1728-1733
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    • 2001
  • An experiment was conducted at the experimental duck farm of Cantho University in the Mekong Delta of Vietnam to evaluate the effects of offering pairwise feeds with different crude protein (CP) levels to Cherry Valley broiler ducks from 28-63 days of age on feed intake and performance. Five diets consisting of mixtures of soya bean meal, fish meal, maize meal and fine rice bran, were formulated to give isocaloric diets with 30, 24, 18, 16 and 12% CP. These were offered separately as pellets to growing ducklings in the following combinations: 30+18, 30+12, 24+16 and 18+12. A diet with 20% CP (CP20) was used as control. All the diets were offered ad libitum. Daily feed intakes (g dry matter, DM) were 161, 168, 150, 101 and 143 g for the 30+18, 30+12, 24+16, 18+12 and CP20 treatments, respectively. CP intakes were 29.6, 27.0, 24.8, 17.1 and 22.9% of total DM intake, and the ratio of methionine+cystine to lysine was 0.76, 0.75, 0.75, 0.76 and 0.75 for the 30+18, 30+12, 24+16, 18+12 and CP20 treatments, respectively. Live weights at 63 days of age were 2,937, 3,054, 2,898, 2,200, and 2,811 g (p<0.001), and daily live weight gains 57.8, 61.7, 57.0, 38.5 and 55.7 g (p<0.001) for the 30+18, 30+12, 24+16, 18+12 and CP20 treatments, respectively. The weights and yields of the carcass, breast and thigh muscles, and heart decreased in the order 30+18, 30+12, 24+16, CP20 and 18+12 (p<0.001). The present study suggests that growing broiler offered a combination of high and low protein feeds pairwise will eat excessive amounts of the high protein feed, thus resulting in protein intakes above requirements.