• Title/Summary/Keyword: 2W

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Effect of plasmid curing on the 2, 3-dihydroxybenzoic acid production and antibiotic resistance of Acinetobacter sp. B-W (Acinetobacter sp. B-W의 2, 3-dihydroxybenzoic acid 생산과 항생제 저항성에 미치는 플라스미드 제거 효과)

  • Kim, Kyoung-Ja;Kim, Jin-Woo;Yang, Yong-Joon
    • Korean Journal of Microbiology
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    • v.52 no.3
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    • pp.254-259
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    • 2016
  • Acinetobacter sp. B-W producing siderophore, 2, 3-dihydroxybenzoic acid (DHB) was analyzed for plasmid content. Strain B-W harbored plasmid of 20 kb in size. Growth at $43^{\circ}C$ was effective in producing mutant cured of plasmid of strain B-W. This mutant lost the ability to produce 2, 3-DHB. Formation of siderophore halos on the chrome azurol S (CAS) agar medium was not detected by cured strain B-W. pHs of supernatants of wild type strain B-W and cured mutant grown in glucose and $MnSO_4$ containing medium at $28^{\circ}C$ for 3 days were 4.5 and 8.5, respectively. Antibiotic resistance against ampicillin, actinomycin D, bacitracin, lincomycin, and vancomycin was lost in cured mutant. Plasmid curing of strain B-W resulted in drastic reduction of minimal inhibitory concentration (MIC) of several antibiotics. E. coli $DH5{\alpha}$ was transformed with plasmid isolated from strain B-W. The transformant E. coli $DH5{\alpha}$ harbored a plasmid of the same molecular size as that of the donor plasmid. Transformant E. coli $DH5{\alpha}$ produced 2, 3-DHB and contained antibiotic resistant ability. Thus a single plasmid of 20 kb seemed to be involved in 2, 3-DHB production. Genes encoding resistance to antibiotics were also supposed to be located on this plasmid.

A Study on Microstructure and Mechanical Properties of Modified 9Cr-1Mo and 9Cr-0.5Mo-2W Steels for nuclear Power Plant (원자력용 개량 9Cr-1Mo 및 9Cr-0.5Mo-2W 강의 미세조직과 기계적 특성 연구)

  • Kim, Seong-Ho;Song, Byeong-Jun;Han, Chang-Seok;Guk, Il-Hyeon;Ryu, U-Seok
    • Korean Journal of Materials Research
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    • v.9 no.11
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    • pp.1137-1143
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    • 1999
  • Microstructure and mechanical properties of Mod.9Cr-1Mo and W added 9Cr-0.5Mo2W steels were investigated for liquid metal reactor (LMR) heat exchange tube. The tempering temperatures at which cell structure was formed were $700^{\circ}C$ for Mod.9Cr-1Mo steel and $750^{\circ}C$ for W added 9Cr0.5Mo-2W steel. indicating the recovery of dislocation was delayed by the addition of W. 9Cr-0.5Mo-2W steel had the same kinds of precipitates with Mod.9Cr-1Mo steel, but the W was included in the precipitates in 9Cr-0.5Mo-2W steel. Micro-hardness and ultimate tensile strength of 9Cr-0.5Mo-2W steel were higher than those of Mod.9Cr-1Mo steel. The impact property of Mod.9Cr-1Mo steel was superior to that of 9Cr-0.5Mo-2W steel.

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Effect of $CO_2$ Laser on Caries Inhibition Evaluated by Laser Fluorescence Measurement (이산화탄소 레이저의 우식 억제 효과에 대한 레이저형광측정 평가)

  • Kim, Seong-Hyeong;Lee, Kwang-Hee;Kim, Dae-Eop;Lee, Ji-Young;Song, In-Kyung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.30 no.1
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    • pp.153-160
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    • 2003
  • The purpose of study was to evaluate the effects of the $CO_2$ laser irradiation on demineralization inhibition and rehardening of human primary tooth enamel by laser fluoresecence measurement. Enamel specimens were made from the human primary teeth. The center spots of the specimens about 2 mm diameter were irradiated by $CO_2$ laser at the conditions of focused continuous or defocused pulsed, 3 or 6 W, for 4 seconds, before or after the demineralization by Coca-Cola for 24 hours at $37^{\circ}C$. The Diagnodent was used to measure the degree of demineralization and rehardening. There was no significant difference between focused continuous and defocused pulsed irradiation. 6W irradiation inhibited the demineralization but 3W did not. 6W irradiation rehardened the demineralized enamel but 3W did partially. The color of enamel was changed to brown to black after 6W irradiation but 3W caused no color change. $CO_2$ laser irradiation showed the effects on demineralization inhibition and rehardening of human priamary tooth enamel, and the laser fluoresecence measurement technique seemed to be a valid evaluation method.

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Drying of By-product Gypsum by Microwave Heating (마이크로파 가열에 의한 부산석고(副産石膏)의 건조 특성)

  • Kim, Hyung-Seok;Chae, Young-Bae;Jung, Su-Bok;Jang, Young-Nam
    • Journal of the Mineralogical Society of Korea
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    • v.21 no.2
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    • pp.193-200
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    • 2008
  • We tried to evaporate and dry the moisture contained Flue gas desulfurization gypsum and phosphogypsum by using the microwave directly. In the result of the heating to the Flue gas desulfurization gypsum and phosphogypsum using 2.45 GHz microwave which was created by magnetron 700 W, 1,000 W and 1,700 W, respectively. According to the increasing the microwave output intensity from 700 W to 1,700 W, the evaporate time of moisture was shortened from 10 to 3 minutes. Gypsum were changed to calcium sulfate hemihydrate. However, ${\beta}$-calcium sulfate hemi-hydrate were not changed to anhydrite.

Metal Injection Molding of Nanostructured W-Cu Composite Powders Prepared by Mechanical Alloying (기계적 합금방법으로 제조한 극초미세 조직의 W-Cu 복합분말의 금속사출성형 연구)

  • 김진천
    • Journal of Powder Materials
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    • v.5 no.2
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    • pp.145-153
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    • 1998
  • W-Cu alloy is attractive to thermal managing materials in microelectronic devices because of its good thermal properties. The metal injection molding (MIM) of W-Cu systems can satisfy the need for mass production of the complex shaped W-Cu parts in semiconductor devices. In this study, the application of MIM process of the mechanically alloyed (MA) W-Cu composite powders, which had higher sinterability were investigated. The MA W-Cu powders and reduction treated (RT) powders were injected by using of the multicomponent binder system. The multi-stage debinding cycles were adopted in $N_2$ and $H_2$ atmosphere. The isostatic repressing treatment was carried out in order to improve the relative density of brown parts. The brown part of RT W-Cu composite powder sintered at 110$0^{\circ}C$ had shown the higher sinterability compared to that of MA powder. The relative sintered density of all specimens increased to 96% by sintering at 120$0^{\circ}C$ for 1 hour. The relationship between green density and the sintering behavior of MA W-Cu composite powder was analyzed and discussed on the basis of the nanostructured characteristics of the MA W-Cu composite powder.

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Synthesis and Characteristics of W-Ni-Fe Nanocomposite Powder by Hydrogen Reduction of Oxides (산화물 수소환원에 의한 W-Ni-Fe 나노복합분말의 합성과 특성)

  • 이창우;윤의식;이재성
    • Journal of Powder Materials
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    • v.8 no.1
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    • pp.49-54
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    • 2001
  • The synthesis and characteristics of W-Ni-Fe nanocomposite powder by hydrogen reduction of ball milled W-Ni-Fe oxide mixture were investigated. The ball milled oxide mixture was prepared by high energy attrition milling of W blue powder, NiO and $Fe_2O_3$ for 1 h. The structure of the oxide mixture was characteristic of nano porous agglomerate composite powder consisting of nanoscale particles and pores which act as effective removal path of water vapor during hydrogen reduction process. The reduction experiment showed that the reduction reaction starts from NiO, followed by $Fe_2O_3$ and finally W oxide. It was also found that during the reduction process rapid alloying of Ni-Fe yielded the formation of $\gamma$-Ni-Fe. After reduction at 80$0^{\circ}C$ for 1 h, the nano-composite powder of W-4.57Ni-2.34Fe comprising W and $\gamma$-Ni-Fe phases was produced, of which grain size was35nm for W and 87 nm for $\gamma$-Ni-Fe, respectively. Sinterability of the W heavy alloy nanopowder showing full density and sound microstructure under the condition of 147$0^{\circ}C$/20 min is thought to be suitable for raw material for powder injection molding of tungsten heavy alloy.

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Flow Resistance and Modeling Rule of Fishing Nets 3. Establishment of Modeling Rule and its Theoritical Examination (그물어구의 유수저항과 모형수칙 3. 모형수칙의 수립 및 이론적 검토)

  • KIM Dae-An
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.4
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    • pp.543-549
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    • 1997
  • The problems in the existing modeling rules for fishing nets, especially in the Tauti's rule which had been used most commonly, were investigated and it was found that the rules could not give a good similarity between the prototype and model nets because they din neither analyze the flow resistance of nets accurately nor decide the ratio of flow velocity between the two nets properly. Thus, the modeling rule was newly derived by regarding the nets as holey structures sucking water into their mouth and then filtering water through their meshes as in the previous paper. The similarity conditions obtained, between the two nets distinguished by subscript 1 and 2, are as follows; $$\frac{d_2}{d_1}=\sqrt{\frac{l_2}{l_1}},\;\frac{N_2}{N_1}=(\frac{d_1}{d_2})^{1.5}\frac{L_2}{L_1},\;\varphi_1=\varphi_2,\;\frac{d_{r2}}{d_{r1}}=\sqrt{\frac{L_2{(\rho_{r1}-\rho_{w1})}}{{L_1{(\rho_{r2}-\rho_{w2})}}$$ $$\frac{N_{a2}}{N_{a1}}=\frac{W_{a1}}{W_{a2}}(\frac{L_2}{L_1})^2,\;\nu_1=\nu_2\;and\;\frac{R_2}{R_1}=(\frac{L_2}{L_1})^2$$, where L is the length of nettings, d the diameter of netting twines, 2l the mesh size, $2\varphi$ the angle between two adjacent bars, N the number of meshes at the sides of nettings, $d_r$, the diameter of ropes, $\rho_r$, the specific gravity of ropes, $W_a$ the weight in water of one piece of float or sinker, $N_a$ the number of floats or sinkers, $\nu$ the flow velocity, and R the flow resistance of net. In the case where the model experiments aim at investigating the influence of weight in water of nettings on their shapes in nets subjected to the water flow of very low velocity, however, the following condition is added; $$\frac{\rho_2-\rho_{w2}}{\rho_1-\rho_{w1}}=\frac{d_1}{d_2}$$ where $\rho$ is the specific gravity of netting twines.

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Evaluation of the Applicability of Azon and Azo-Core for Enhancing Airtightness and Thermal Insulation Performance of Insulated Shutters (단열 셔터의 기밀성능 및 단열성능 확보를 위한 Azon 및 Azo-Core의 적용성 평가)

  • Kil, Min-Woo;Kim, Gyu-Yong;Choi, Byung-Cheol;Ji, Sung-Jun;Youn, Hye-Young;Nam, Jeong-Soo
    • Journal of the Korea Institute of Building Construction
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    • v.24 no.4
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    • pp.437-445
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    • 2024
  • This study investigated the thermal insulation performance of Azon and Azo-Core, two materials commonly used in window frames, for potential application in window shutters. A three-pronged evaluation approach was employed, utilizing the Therm 7.4 software, confidential test results, and dedicated thermal insulation testing. The simulation data indicated that both Azon and Azo-Core offered superior insulating properties compared to conventional shutter insulation materials. When incorporated into shutters, these materials achieved a first-grade sealing performance and a measured average thermal conductivity of 2.018W/m2·K. While this thermal conductivity value surpasses the standard requirements of 1.5W/m2·K for central regions and 1.8W/m2·K for southern regions in Korea, it falls within the acceptable limit of 2.2W/m2·K established for the Jeju region. Based on these findings, Azo-Core, warranting further dedicated research, presents itself as a promising candidate for shutter insulation material, particularly in applications targeting the Jeju region.

STUDIES ON THE BIOLOGY OF THE SPRING SPAWNING GROUPS OF PENAEUS JAPONICUS BATE (보리새우 Penaeus japonicus Bate의 춘계 산란군에 관한 생물학적 연구)

  • PYEN Chnng Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.4
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    • pp.219-227
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    • 1970
  • The shrimp fishery for Penaeus japonicus on the Koje-Do coast commences in May and continues to October. The best catches are made twice a year, namely June and September. Modal carapace length for the female is $51\~55\;mm$ in May, $51\~60\;mm$ in June occupying about 80 percent of the total catch. There is conspicuous mode in July and $46\~50\;mm$ in August. Modal carapace length for the male is $41\~45\;mm$ in May, $46\~50\;mm$ in June, $41\~45\;mm$ in July and August. The stock of p. japonicus can be divided into two groups, namely, spring and fall groups. Among samples of p. japonicus during the period from May through August the sex ratio consisted of about 33 percent females and 67 percent males, showing wide departure from a 1:1 ratio. The relationships between carapace length ($\iota$), body length (L) and body weight (W) are expressed by the following formulae : $$L=2.9418{\iota}+2.0166$$, $$W:1.449{\iota}^{2.2858}$$ (Mayssample) $$L=2.5551{\iota}+4.2986$$, $$W=1.528{\iota}^{2.2595}$$ (June sample) $$L=2.6738{\iota}+3.4037$$, $$W=1.341{\iota}^{2.3598}$$ (July sample) The relatlonship between carapace length ($\iota$) and ovary weight (W) is shown as follows: $W=2.695\times10^{-3}\iota^{4.2973}$ for May, June and July Samples.

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