• Title/Summary/Keyword: 2DGE

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Physiological and proteomic analysis of young rice leaves grown under nitrogen-starvation conditions

  • Kim, Sang-Gon;Wang, Yiming;Wu, Jingni;Kang, Kyu-Young;Kim, Sun-Tae
    • Plant Biotechnology Reports
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    • v.5 no.4
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    • pp.309-315
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    • 2011
  • Rice grown in anaerobic waterlogged soil accumulates ammonium as a major source of nitrogen (N). We have compared the physiological symptoms of rice seedlings subjected to N-starvation stress with those receiving sufficient N, based on measurements of shoot/root length and weight and an analysis of protein expression patterns. N starvation marginally increased root growth but notably decreased shoot biomass. N uptake was reduced by >50% in the roots and shoots of N-starved seedlings. To better understand the mechanism of N starvation in rice, we performed a comparative proteome analysis of proteins isolated from rice leaves. Twenty-five differentially expressed proteins were analyzed by matrixassisted laser desorption/ionization time-of-flight (TOF) mass spectrometry and electron spray ionization quadrupole TOF. Functional analysis of the N-starvation response proteins suggested their involvement in protein synthesis and fate, metabolism, and defense. These results indicate that these proteins may play important roles in regulating the plant's complex adaptation responses for N use during N starvation. The proteins may be useful for further characterization of protein function in plant N nutrition.

A Study on the Damage to a Concrete Bridge Pier due to Fire (화재를 입은 콘크리트 교각의 손상에 관한 연구)

  • 윤우현
    • Magazine of the Korea Concrete Institute
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    • v.7 no.2
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    • pp.117-125
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    • 1995
  • In this study, the damage to a concrete br~dge pier due to flre caused by the fall of an oil truck were investigated by the use of FEM and by tensile tests for reinfortements. And thtse results were analyzed and compared with the measured values. In the FEM calculations, the selected variable was the fire temperature $T_a=500-800^{\circ}C$. The fixed values were the heat transition coefficient ${\alpha}=2000W/m^2{\cdot}K$. the initial temperature of concrete $T_0=5{\circ}C$ and the fire duration t=30 minutes. As the results obtained from numerical calculations, the property darrlage zone ap,)eared to be 1.5-4.1cm and the structure damage zone appeared to be 8.7- 10.1cm from the concrete surface. And this results give values very similar to those measured, nanlelv 2-4cm and 8~10cm respectively. The results frorn tensile tests give no serious loss of the tensile strength.

Web-Based Alcohol Preventive Education (웹기반 음주예방 교육)

  • Jeong, Mun-Hui;Jeon, Hye-Jeong;Kim, Mi-Hye;Kim, Yeong-Mi;Lee, Geum-Sun
    • Journal of the Korean Society of School Health
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    • v.19 no.2
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    • pp.117-125
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    • 2006
  • Purpose : The purpose of this study is to test the effect of the web-b ased alcohol preventive education for ndicate the model of alcohol preventive education for students spreading al over the family and the comunity.Methods : This study was performed on the 1 public and 1 private eleme ntary school students who are the six grade in Seongdong-gu, Seoul. The number of experimental gr oup was 117 and control group's number was 131, totaled 248. eeks. The pre- and the post-test for web-based alcohol preventive education were tested about knowle dge and attitude toward drinking by questionnaire. The data analysis was done using SPS/Win 12.0 p rogram.Results:The results of this study are as follows;1. Compared with control group, experimental group which was educated the web-based alcohol preventive icant diference in the knowledge toward drinking.2. Compared with control group, also the experimental group w as improved with significant diference in attitude toward drinking after the education.3. In control group, female students' knowledge of drinking w as more increased than male students. And the he group which experienced drinking.Conclusions : The web-based alcohol preventi ve education is worth as a int ervention to get positive influence for the health education. Especially in female studen ts who didn't met with drinking, early education for alcohol prevention is very important. Therefore i t will be helpful that coperate with district ofice ithin the comunity for becoming established the controled drinking pattern.

Quantitative importance and species composition of polychaetes in the benthic community of the Yellow Sea (황해의 저서군집내에서 다모류의 중요성과 종조성)

  • LEE Jae-Hac;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.22 no.4
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    • pp.189-195
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    • 1989
  • The ecological studies of the benthic polychaetes were carried out in the follow Sea for the period of August 1982 through February 1986. Special emphasis were placed on quantitative species composition and the importance of polychaetes in the benthic community. Polychaete group ranked numerically high, composing $35.1\~46.4\%$ of the total benthos. In the biomass, echinoderms or mollusks showed as a dominant group, but the range of its seasonal variation was very large. Due to the use of sampling gear, the relative importance of polychaetes was somewhat different. The case of a dredge showed a little lower composition rate of polychaetes than a grab one. This result was caused by high density of benthic animals because the dredge would tow only much more surface sediments than the grab sampler. The present studies included a total of 141 species and the representative polychaetes in this area were Spiophanes bormbyx, Ampharete arctica, Geniada maulata, Nephtys caeca, Nothria iridescens.

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A Study for the Relationship between the BMI and the Eating Disorder, Nutritional Knowledge among Female Middle School Girls in Won Ju Province (원주지역 여중생들의 BMI와 이상식이습관, 영양지식과의 관련성)

  • Oh, Hae-Sook;Won, Hyang-Rye;Rhie, Seung-Gyo
    • The Korean Journal of Community Living Science
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    • v.18 no.1
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    • pp.97-105
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    • 2007
  • This study was carried out to find the relationship between the eating disorder associated with BMI(body mass index) and nutrition knowledge by surveying 476 middle school girl students in Won Ju, Gang-Won Province, South Korea. BMI is a standard suggested for oriental people and it was differentiated into the underweight, the normal weight and the overweight. To determine the eating disorder, EAT-26(Eating Attitude Test-26) was used and in order to find out the level of nutrition knowledge DGE (Deutsche Gesellschaft fur Ernahrung) was used after some modifications and supplementations. And the results are as follows; 1) Out of the students surveyed, 22% are in underweight group, 64% are in normal weight group and just 12% are in overweight group. Except the students in underweight group, most of the surveyed students wish to lower the weight below normal weight. 2) Relationship was found between BMI and EAT-26 score that is related with eating disorder behavior, Total score was higher in overweight group than in underweight and normal weight group (P<0.001). When the score for individual question about eating disorder is considered in detail, the score for the questions preoccupied with the weight reduction was higher in overweight group than in underweight and normal weight group(P<0.001). For the questions about bulimia there was no difference according to BMI, however, for the questions about restraining food intake the score was high in overweight group(P<0.001). 3) The score of nutrition knowledge in line with BMI was different. It was found that the total score and the score for the questions about fat, calorie requirement, and calorie content in food were different. The total score showed the highest in overweight group in comparison with the ones in underweight and normal weight group. (P<0.05). Out of maccronutrient like carbohydrate, fat and protein, only the fat showed significant difference according to BMI and it was lower in overweight group than in underweight and normal weight group.(P<0.05) Out of the questions about calorie requirement, significant difference was found in the score for the questions about calorie requirement and calorie content in food. It was lower in overweight group than in underweight and normal weight group (p<0.05).

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A Study on Third-trimester Gravidas' Konwledge of Infant Care Activity (임신 말기 임신부의 영아 돌보기 지식에 관한 연구)

  • Lee, Su-Yeon
    • The Korean Nurse
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    • v.29 no.3
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    • pp.64-78
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    • 1990
  • The purpose of this study was to measure the degree of gravidas' knowledge of infant care activity, and to identify the factors influencing the difference of gravidas' knowledge. The subjects of this study consisted of 159 gravida visited 2 general hospitals, 2 OB/GY clinics and 2 midwives' clinics in J city for anenatal care. The data were collected from December 1 to 30, J.989. The instrument used for this study was true false type question which was developed by investigator through literature review. Data were analyzed by percentage, mean, t-test and ANOVA on significant difference with SPSS program. The results of this study were, summarized as follow: 1) The degree of gravidas' knowledge of infant care activity was 36.75, the degree of gravidas' know ledge of physical care activicy was 16.70, and the degree of gravidas' knowledge of psychosocial care activity was 20.00. 2) Among the physical care activity knowledge items, the diaper should be changed whenever it is soiled, $\lceil$shortly after feeding is over, bathing is bad$\rfloor$, $\lceil$gently patted or stroked on the back when bubbling$\rfloor$, $\lceil$10 minutes time in bathing is appropriate$\rfloor$, $\lceil$infant is crying whenever he is hungry per 3-4 hours$\rfloor$, and "room humidity controled 60 percent, more or less> were over a' percentage of 80 of right answer. Among the physical care, accivity knowledge items. $\lceil$a reason for burping was the air' he has swallowed will rise to the top of his stomach and be eructated$\rfloor$, $\lceil$burping is advisable after the feeding$\rfloor$, $\lceil$dressing of umbilical area is not necessary$\rfloor$, $\lceil$the thermometer should not be boiled for disinfection$\rfloor$, $\lceil$it is important chat the infant grasp the whole nipple within his mouth$\rfloor$, $\lceil$using alcohol sponge is bad whenever diaper is soiled", and $\lceil$when temperature is taken by the rectal method, infant legs shoud be grasped firmly_! were less than a percentage of 60 of right answer. 3) Among the psychosocial care activity knowledge items, $\lceil$it is nice, mother smile at bady frequently$\rfloor$, $\lceil$it is nice, mother praise a bady frequently$\rfloor$, $\lceil$it is nice, mother express bady's behavior$\rfloor$, $\lceil$talk frequently to the bady, the bady :should not be disturbed while feeding$\rfloor$, $\lceil$it is nice, mother see a baby face to face , $\lceil$it is nice, mother concentrate one's attention on her baby while playing$\rfloor$, $\lceil$it is nice, mother pat one's baby while he is feeding$\rfloor$, $\lceil$it is advisable, the baby is allowed to touch mother's breast while he is feeding$\rfloor$, $\lceil$it is advisable to stop feeding, if the baby protrudes one's tongue or cries while feeding$\rfloor$, $\lceil$baby is able to hear, see and smell$\rfloor$, $\lceil$it is nice, mother Concentrate one's attention on her baby while feeding$\rfloor$, and $\lceil$it is not advisable for mother to scold baby while he is crying out> were over a percentage of 80 of right answer. Among the psychosocial care activity knowledge items, $\lceil$Using' baby talks is bad when mother talks to baby> and $\lceil$it is nice, mother soothes the baby as soon as possible when he is crying I were less than a percentage of 60 of right answer. 4) There was statistically significant relationship between the degree of gravidas' knowledge of infant care activity and the demographic variables of gravida such as the age(p<0.01) and education level(p<0.01). From these results, it may be concluded that gravidas' knowledge of infant care activity is moderate level, and gravidas' knowledge of psychosocial care activity is greater than that of physical care activity. Also prenatal nursing educations should focus on subjects who have lower percentage of right answer in infant care activity knowledge items.dge items.

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