• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5825-5831
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• 2013

Background/Objectives: Maintenance of cellular function in culture is vital for transfer and development following adoptive immunotherapy. Dual properties of IL-21 in activating T cells and reducing activation induced cell death led us to explore the mechanism of action of IL-21 enhanced proliferation and cytotoxic potential of CIK cells. Method: CIK cells cultured from PBMCs of healthy subjects were stimulated with IL-21 and cellular viability and cytotoxicity to K562 cells were measured. To elucidate the mechanism of action of IL-21, mRNA expression of cytotoxic factors was assessed by RT-PCR and protein expression of significantly important cytotoxic factors and cytokine secretion were determined through flow cytometry and ELISA. Western blotting was performed to check the involvement of the JAK/STAT pathway following stimulation. Results: We found that IL-21 did not enhance in vitro proliferation of CIK cells, but did increase the number of cells expressing the CD3+/CD56+ phenotype. Cytotoxic potential was increased with corresponding increase in perforin ($0.9831{\pm}0.1265$ to $0.7592{\pm}0.1457$), granzyme B ($0.4084{\pm}0.1589$ to $0.7319{\pm}0.1639$) and FasL ($0.4015{\pm}0.2842$ to $0.7381{\pm}0.2568$). Interferon gamma and TNF-alpha were noted to increase ($25.8{\pm}6.1ng/L$ to $56.0{\pm}2.3ng/L$; and $5.64{\pm}0.61{\mu}g/L$ to $15.14{\pm}0.93{\mu}g/L$, respectively) while no significant differences were observed in the expression of granzyme A, TNF-alpha and NKG2D, and NKG2D. We further affirmed that IL-21 signals through the STAT-3 and STAT-5b signaling pathway in the CIK cell pool. Conclusion: IL-21 enhances cytotoxic potential of CIK cells through increasing expression of perforin, granzyme B, IFN-gamma and TNF-alpha. The effect is brought about by the activation of STAT-3 and STAT-5b proteins.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.2
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• pp.203-209
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• 2013

Digital Radiography (DR) has improved a quality of resolution based on a wide dynamic range, high detective quantum efficiency (DQE), and modulation transfer function (MTF), compared with film/screen(F/s). Unlike expectation that a low level of radiation can be used in examination, high level of signal to noise ratio(SNR) due to over-exposure caused increase of exposed dose to patients. Also, the auto exposure control (AEC) using Kilovolage(kVp) in F/S can cause over-exposure. Hence, in this study, we proposed a proper method for using DR, in which effect of tubing Kilovolage on device's image, DR MTF measurement with changes of tubing current (mA), and the quantitative evaluation of skull phantom captured images' PSNR were evaluated. Changes of contrast with tubing Kilovolage can be improved by retouching, and MTF changes according to tubing current(1.41~1.39 lp/mm in 50% area, and 3.19~2.8 lp/mm in 10% area) does not influence on resolution of image. As a result, high tubing Kilovoltage, and tubing current will be suitable to use of DR.

• Transactions of the Korean Society of Automotive Engineers
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• v.19 no.1
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• pp.58-65
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• 2011

The specific objective of this study was to develop an IRDS (integrated receiver drier subcooling) condenser model for use in a mobile air-conditioning system. A three-zone model based on the desuperheating, two-phase, and subcooling sections of a condenser could be used to estimate the performance with a good accuracy. Overall heat transfer coefficients for each of the three sections, expressed as a function of the air velocity across the condenser and refrigerant mass flow rate and the model using the elemental difference method incorporate calculations to determine the pressure drop, heat performance within the condenser and it includes physical parameters (pass, tube hole size and length) that can be varied to analyze potential design changes without exhaustive experimental efforts. it was found that an accuracy of heat performance was within 5% in case of using the various condensers, the refrigerant pressure drop was predicted within 25% and the pressure drop of air side was well matched with experiment data within 4%.

• Journal of Biosystems Engineering
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• v.34 no.5
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• pp.305-314
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• 2009

The purpose of this paper was to design an effective control system of 1-axis exciter for a seat vibration test of agricultural tractors using MATLAB simulation. The developed simulation model was composed with a hydraulic pump, a hydraulic servo valve, a hydraulic cylinder and load system. Also it was verified by comparing the simulation results with experimental results of actual control system in order to optimize the control performance. And in order to improve its control performance, the designed PID controller in this research was tuned using Ziegler-Nichols 2nd law and zero's moving method of PID controller's transfer function. As the result of these research, the developed position control system was able to control the system's position accurately within 5% errors.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.9 s.174
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• pp.147-154
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• 2005

A precision hi-directional actuator for a high precision leveling system with $Z{\Theta}_x{\Theta}_y$ motions is proposed and designed in this paper. The actuator is composed of a force generation structure, a guide mechanism, and a symmetric structure. At first, its driving force is generated by a change of flux in air gaps by permanent and changeable flux. The permanent flux is generated by a permanent magnet. The changeable flux is created by variable current flowing through coil. The combination of permanent and changeable flux makes various flux densities in air gaps between moving part and fixed yokes. And then, the difference between flux densities in lower and upper gaps creates forces fur the $bi-direction({\pm}z)$ motion. The guide mechanism of this actuator is composed of two circular plates and one shaft. Reducing motions generated by forces except z-motion, these circular plates endow the actuator with high stiffness for fast settling time. And the function of the shaft is to transfer motion to an object. At last, total body has a symmetric structure to be stable on thermal error. The actuator is designed by MAXWELL 2D and ProMECHANICA. The designed actuator is evaluated by 8nm laser doppler vibrometer, dynamic signal analyzer, and simple PID controller.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.5
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• pp.1700-1716
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• 1996

The two-dimensional, unsteady, laminar conservation equations for mass, momentum, energy and species transport in the gas phase and mass, momentum and energy in the liquid phase are solved simultaneously in spherical coordinates in order to study heating and vaporization of a droplet entrained in the oscillating flow. The numerical solution gives the velocity and temperature distribution in both gas and liquid phase as a function of time. When the gas flow oscillates around an vaporizing droplet, the liquid flow circulates in the clockwise or counterclockwise direction and the temperature distribution in the liquid phase changes its shapes, depending on the gas fow direction. When the gas flow changes its direction of circulating liquid flow is opposite to the gas flow, forming two vortex circulating in the opposite direction. During the heating period, the difference in the maximum and minimum temperature is large, followed by the almost uniform temperature slightly below the boiling temperature. The mass and heat transfer from the droplet depend on the droplet temperature, droplet diameter and the magnitude of relative velocity, giving the droplet lifetime different from the d$^{2}$-law.

• Proceedings of the Acoustical Society of Korea Conference
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• spring
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• pp.141-146
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• 1999

This study aims at establishing an digital signal processing technique to control 3-D sound localization, especially focusing our eyes on the role of information provided by Head-Related Transfer Function(HRTF). In order to clarify the cues to control the auditory distance perception, two conventional models named Hirsch-Tahara model and auditory parallax model were examined. As a result, it was shown that both models have limitations to universally explain the auditory distance perception. Hence, the auditory parallax model was extended so as to apply in broader cases of auditory distance perception. The results of the experiment by simulating HRTFs based on the extented parallax model showed that the cues provided by the new model were almost sufficient to control the perception of auditory distance from an actual sound source located within about 2 m.

• Journal of the Korean Home Economics Association
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• v.35 no.5
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• pp.249-264
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• 1997

The purpose of this study was to provide the reference information for user and potential users of apparel CAD system is Korea. Two interviews were carried out for the study. The apparel CAD system of Assyst, Gerber and Yuka was selected for technical comparative study. The results were as follows: 1. The future development of the apparel CAD system is the transfer of the developed pattern design from a 3D design system and of CIM concept. 2. The share of data is working closely in a module function. So the Assyst system provide connectivity and communication between all apparel CAD system's module and other automation programs. This system is suitable for CIM production line. 3. The Gerber system is developed the Apparel CAD system which is given by the CAM system's technical ability. This system is given high insurance of ability to the service and data transportation with other systems from users in the Korea. 4. The Yuka system is developed pattern making by the Apparel CAD system. This system's different methods which is compared with other systems are the split grading and auto pattern making. So this system is suitable for users which want to product many items and a little amount garment by using the Apparel CAD system.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.12
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• pp.1652-1661
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• 2000

A visualization study using the schlieren method is adopted in an optically-accessible, cylindrical constant volume combustion chamber to identify the mechanism of ignition energy and ignition system interaction in spark ignited, lean gasoline-air mixture. In order to research the effects of ignition system on flame propagation, two kinds of ignition system are designed, and several kinds of spark plugs are tested and evaluated. To control the discharge energy, the dwell time is varied. The initial flame development is quantified in terms of 2-D images which provides information about the projected flame area and development velocity as a function of ignition system and discharge energy. The results show that high ignition energy and extended spark plug gap can shorten the combustion duration in lean mixtures. The material, diameter and configuration of electrodes the flame development by changing the transfer efficiency from electrical energy to chemical energy and discharge energy. However these factors do not affect of flame development as much a ignition energy or extended gap does.

• The Journal of the Acoustical Society of Korea
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• v.23 no.1
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• pp.30-39
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• 2004

This study aims at establishing a digital signal processing technique to control 3-D sound localization, especially focusing our ores on the role of information provided by Head-Related Transfer Function (HRTF). In order to clarify the cues to control the auditory distance perception, two conventional models named Hirsch-Tahara model and auditory parallax model were examined. As a result, it was shown that both models have limitations to universally explain the auditory distance perception. Hence, the auditory parallax model was extended so as to apply in broader cases of auditory distance perception. The results of the experiment by simulating HRTFs based on the extended parallax model showed that the cues provided by the new model were almost sufficient to control the perception of auditory distance from an actual sound source located within about 2m.