• Title/Summary/Keyword: 2D solid

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Plant Regeneration from Protoplasts Isolated through Embryogenic Cell suspension Culture in Rice (벼 현탁배양을 통하여 분리된 원형질체로부터 식물체 재분화)

  • 정병균
    • Journal of Plant Biology
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    • v.36 no.3
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    • pp.211-218
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    • 1993
  • Plant regeneration was accomplished from protoplast culture of rice (Oryza sativa L. cv. Taebaeg). Embryogenic callus was induced from mature seed on MS medium containing 5 mM proline, 2.5 mg/L 2,4-D, 30 g/L sucrose in the dark at 28$^{\circ}C$ and used to establish embryogenic cell suspension culture. Suspension cells were subcultured every one week in N6 medium supplemented with 5 mM proline, 200 mg/L casein hydrolysate, 2.5 mg/L 2,4-D and amino acids of AA medium. Suspension cultures were composed of cells that were densely cytoplasmic, potentially embryogenic and were at least maintained for more than 6 months in liquid medium. Protoplasts were isolated from fast-growing suspension culture cells and cultured in a slightly modified KpR medium by mixed nurse culture. Isolated protoplasts began to divide within 5~7 days and thereafter, protoplast-derived calli were sequentially transferred to callus proliferating medium that soft agar MS medium contained 2 mg/L 2,4-D and produced distinct embryogenic cells. Microcolonies were then transferred to solid medium which consisted of MS medium containing 5 mg/L kinetin, 1 mg/L NAA, 1 mg/L ABA, 30 g/L sucrose and 10 g/L sorbitol under fluorescent light. Mulitple shoots of 4~5 per callus emerged and were transferred to hormone-free MS medium for root initiation. Thereafter, The plantlets were transferred to pots of soil to mature in the culture room.

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Development and Verification of AMBIKIN2D, A Two Dimensional Kinetics Code for Fluid Fuel Reactors (유동핵연료원자로를 위한 이차원 동특성 코드 AMBIKIN2D 개발 및 검증)

  • Lee, Young-Joon;Oh, See-Kee
    • Journal of Energy Engineering
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    • v.17 no.1
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    • pp.23-30
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    • 2008
  • The neutron kinetic analysis methods for the molten-salt reactors are quite different from those for conventional solid-fuel reactors, which do not take into account the flowing-fuel-induced neutronics effects. Therefore, for dynamics and safety analyses of the molten-salt reactor systems, the conventional kinetics codes would not be appropriate to accurately predict its transient behaviors. A point-kinetics with flowing- fuel model has been used to assess the fluid-fuel reactor system safety, but recognized as not to be sufficient to simulate spatial distributions of delayed-neutron precursors and neutron populations during transients for given detail reactor models. In order to meet this requirement, AMBIKIND, a 2-group, 2-dimensional neutron kinetics code suitable for the molten-salt reactor systems was developed. This paper explains the code's theoretical and numerical descriptions and, as a part of its verification, includes some simulation results of MSRE stability experiments. Even though the present reactor model does not include the recirculation effect of the fuel-salt through the reactor system, the AMBIKIN2D code should be able to predict the power and phase shift at various power levels and reactivity insertions with better accuracy.

Development of Solid Culture Media for the Mass Production of Beauveria bassiana Conidia (흰굳음병균(Beauveria bassiana) 포자의 대량배양을 위한 고체배지의 개발)

  • 서종복;진병래
    • Journal of Sericultural and Entomological Science
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    • v.38 no.1
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    • pp.31-35
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    • 1996
  • To develope a solid culture media for the mass culture of Beauveria bassiana conida, wheat bran was selected as C source and N source. The pellet media were prepared without(P-I) and with(P-II) solidified ingredient. The moisture for the growth of B. bassiana was required over 4 : 2 of Media : D.W (W/V). The growth of Beauveria bassiana on the media type was more effective in media of pellet type than that of powder type. In addition, production of Beauveria bassiana conidia on the media size was more effective in media of S type($\Phi$3mm) than that of L type($\phi$7mm). And the yield of Beauveria bassiana conidia at 2$0^{\circ}C$ cultivation on 3 weeks post inoculation was similar to that of $25^{\circ}C$. Above-mentioned results showed that pellet medium (P-I) was effective to growth of Beauveria, suggesting that the pellet media may be useful in the mass production of Beauveria bassiana conidia.

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A Study on Rheological Changes of Redbean Jam druing Storage (단팥의 저장 중 물성변화에 관한 연구)

  • Lee, Jeong-Hoon;Shin, Ho
    • The Korean Journal of Food And Nutrition
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    • v.11 no.3
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    • pp.283-288
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    • 1998
  • The changes of the rheological properties of redbean jam were investigated during storage. The relationship between moisture content (40, 45 and 50%) and agar concentration (1, 0.1 and 0.2%) were studied for 5 days of storage. Water holding capacity was measured by placing redbean jam on Toyo Filter paper(No. 5A) and moisture movement was measured by placing redbean jam in bread. Sensory characteristics were examined in terms of color, flavour, preference, bitterness and taste. The results were as follows. Water holding capacity of redbean jam was increased as the solid content of sugar and agar concentration increased. Moisture movement was decreased as the solid content of sugar and agar concentration increased. Color, flavour, preference and taste were increased for the first day but decreased from the second day. There was significant difference(P<0.01). Bitterness did not show significant difference. 45% of moisture content, 0.1% of agar concentration and the first day showed the highest preference.

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Application of Anaerobic Sequencing Batch Reactor to Mesophilic Digestion of Municipal Sewage Sludge (중온 혐기성 연속회분식 공정에 의한 도시하수슬러지의 소화가능성 평가)

  • Hur, Joon-Moo;Chang, Duk;Chung, Tai-Hak;Son, Bo-Soon;Park, Jong-An
    • Journal of Environmental Health Sciences
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    • v.24 no.2
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    • pp.9-19
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    • 1998
  • Laboratory experiments were carried out to investigate the performance of anaerobic sequencing batch reactor(ASBR) for digestion of a municipal sludge. Each cycle of the ASBR comprised feeding, two-or three-day reaction, one-day thickening, and withdrawal. The reactors were operated at an HRT of 10days and 5days with an equivalent organic loading rate of 0.8-1.54 gVS/l/d, 1.81-3.56 gVS/l/d at 35$\circ$C, respectively. Solids accumulation was remarkable in the ASBR during start-up period, and directly affected by settleable solids in the feed sludge. Floatation thickening occured in the ASBRs, and Solids profiles at the end of thickening step dramatically changed at solid-liquid interface. Slight difference in solids concentrations was observed within thickened sludge bed. Efficiencies through floatation thickening were comparable to that of additional thickening of the completely mixed control reactor. Average solids concentrations in the ASBRs were 2.2-2.6 times higher than that in the control throughout the total operation period. The dehydrogenase activity had a strong correlation with the solids concentration. Organics removals based on clarified effluent of the ASBRs were consistently above 86%. Remarkable increase in equivalent gas production of 27-52% was observed at the ASBRs compared with the control though the control and ASBRs showed similiar effluent quality. Thus, digestion of a municipal sludge was possible using the ASBR in spite of high concentration of solids in the sludge.

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Solid Foods Intake Pattern During the First 6 Months of Life (영아 전반기 영양공급방법에 따른 이유보충식의 섭취 양상)

  • 배현숙;안홍석
    • Korean Journal of Community Nutrition
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    • v.1 no.3
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    • pp.335-345
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    • 1996
  • The solids(supplementary food) consumption of 200 healthy infants aged from 4 to 6 months have been measured. Five groups were assigned to different feeding patterns. Breastfed group(BF, n=38), formula-fed group(FF, n=102) and mixed-fed group(MF, n=14) were fed breast milk, formula and mixed(breast mile+formula) from birth until 6 months of age respectively. Convert 1 group(C1F, n=14) and convert 2 group(C2F, n=32) were fed breast milk and mixed milk at 2 months of age respectively and were switched to formula thereafter. All infants received solids form 4 months of age. The energy intake from solids at 4 and 6 months averaged 59.1 and 110.3㎉/d among BF groups vs 151.9 and 239.3㎉/d among FF groups respectively. Intakes of protein, calcium and iron were 38-46$\%$, 34-44% and 25-37$\%$ higher in the FF than in the Bf group during the first 6 months. However, no significant differences were found in the intakes of energy and nutrient intakes from solids in all 4 groups excluding BF group(39$\%$). Although BF infant's nutrient intakes from solids were lower than other group's intakes, they intaked evenly solids from various food groups. As the age of infant had increased, the energy intake ratio from solids was cereals and dairy products was increased whereas energy intake ratio from meats, eggs, fruits and vegetables was decreased. TSC4, TSC6(the frequency score for the kinds of weaning food taken) showed no significant difference among 5 feeding groups but CIF-and C2F group's scores tended to be high. Consequently, these results suggest that BF group's solid intake pattern is more desirable and that Korean lactating Mother's dependence on commercial weaning food is too high.

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Synthesis of Methoxy Polyoxyethlene Dodecanoates (Methoxy Polyoxyethylene Dodecanoate의 합성)

  • Kang, Yun-Seog;Noh, Sueng-Ho;Choi, Seung-Ok;Nam, Ki-Dae
    • Applied Chemistry for Engineering
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    • v.9 no.5
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    • pp.749-753
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    • 1998
  • Methoxy polyoxyethylene dodecanoates, kinds of nonionic surfactants, could be obtained from addition of ethylene oxide (5, 7, 9, and 12mol) with fatty acid methyl ester utilizing solid catalyst, metal oxide. Because ethylene oxide (EO) couldn't react directly in acid or alkali catalyst with dodecanoic acid methyl ester (DME) that had no active hydrogen, the reaction to add EO was carried out using active solid catalyst. By using IR, HPLC and $^1H$ NMR analysis, structural confirmation of methyl polyoxy ethylene dodecanate showed high yield ranging from 93 to 97%. EO unit mol number of reacted products was 5.2, 7.1, 9.2 and 12.1 mol respectively. Also, EO adduct distrobution of ethoxylated methyl laurate (MPD) had normal distribution curve like polyoxyethylene alkyl ether (AE).

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Comparison of Sulfate Reduction Rates Associated with Geochemical Characteristics at the Continental Slope and Basin Sediments in the Ulleung Basin, East Sea (동해 울릉분지에서 대륙사면과 분지 퇴적물의 지화학적 특성에 따른 황산염 환원 비교)

  • You, Ok-Rye;Mok, Jin-Sook;Kim, Sung-Han;Choi, Dong-Lim;Hyun, Jung-Ho
    • Ocean and Polar Research
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    • v.32 no.3
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    • pp.299-307
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    • 2010
  • In conjunction with geochemical characteristics, rate of sulfate reduction was investigated at two sediment sites in the continental slope and rise (basin) of the Ulleung Basin in the East Sea. Geochemical sediment analysis revealed that the surface sediments of the basin site (D2) were enriched with manganese oxides (348 ${\mu}mol$ $cm^{-3}$) and iron oxides (133 ${\mu}mol$ $cm^{-3}$), whereas total reduced sulfur (TRS) in the solid phase was nearly depleted. Sulfate reduction rates (SRRs) ranged from 20.96 to 92.87 nmol $cm^{-3}$ $d^{-1}$ at the slope site (M1) and from 0.65 to 22.32 nmol $cm^{-3}$ $d^{-1}$ at the basin site (D2). Depth integrated SRR within the top 10 cm depth of the slope site (M1; 5.25 mmol $m^{-2}$ $d^{-1}$) was approximately 6 times higher than that at the basin site (D2; 0.94 mmol $m^{-2}$ $d^{-1}$) despite high organic content (>2.0% dry wt.) in the sediment of both sites. The results indicate that the spatial variations of sulfate reduction are affected by the distribution of manganese oxide and iron oxide-enriched surface sediment of the Ulleung Basin.

Three Dimensional Direct Monte Carlo Simulation on OLED Evaporation Process (유기EL 증착 공정에 대한 3차원 Monte Carlo 해석)

  • Lee, Eung-Ki
    • Journal of the Semiconductor & Display Technology
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    • v.8 no.4
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    • pp.37-42
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    • 2009
  • The performance of an OLED(organic luminescent emitting device) fabrication system strongly depends on the design of the evaporation cell-source. Trends in display sizes have hauled the enlargement of mother glass substrates. The enlargement of substrates requires the improvement and the enlargement of the effusion cell-source for OLED evaporation process. The deposited layers should be as uniform as possible, and therefore it is important to know the effusion profile of the molecules emitted from the cell-source. Conventional 2D DSMC algorithm cannot be used for simulating the new concept cell-source design, such as a linear source. This work concerns the development of 3D DSMC (direct simulation Monte Carlo) analysis for simulating the behavior of the evaporation cell-sources. In this paper, the 3D DSMC algorithm was developed and the film thickness profiles were obtained by the numerical analysis.

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Eco-friendly Solventless Synthesis of 5-Indolylpyrimido[4,5-d]pyrimidinones and Their Antimicrobial Activity

  • Gupta, Ragini;Jain, Anshu;Joshi, Rahul;Jain, Meenakshi
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.899-904
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    • 2011
  • A series of 5-indolylpyrimido[4,5-d]pyrimidinones (4a-h) were obtained by multi-component reaction of 3-formylindole (1), thiobarbituric acid/barbituric acid (2) and thiourea/urea (3) under microwave irradiation in dry media. Secondly, grinding together neat reactants also gave the titled compounds in good yields. All the synthesized compounds have been characterized on the basis of elemental analyses and spectral data (IR, $^1H$ NMR and Mass). Representative compounds were also evaluated for their antimicrobial activity against Rhizopus stolonifer, Fusarium oxysporum, Escherichia coli, and Pseudomonas aeruginosa at different concentrations. Some of the compounds showed promising activity.