• Journal of Photoscience
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• v.9 no.2
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• pp.110-113
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• 2002

We demonstrate here a dynamic structure of bacteriorhodopsin (bR) as revealed by $^{13}$ C NMR studies on [3_$^{13}$ C]_,[1-$^{13}$ C]Ala- and/or Val-labeled wild type and a variety of site-directed mutants at ambient temperature. For this purpose, well-resolved (up to twelve) I$^{13}$ C NMR peaks were assigned with reference to the displacement of peaks due to the conformation-dependent I$^{13}$ C chemical shifts and reduced peak-intensities due to site-directed mutations. Revealed bR structure was not rigid as anticipated from 2D crystals of hexagonal array but a dynamically heterogeneous, undergoing a variety of local fluctuations depending upon specific site with frequency range of 10$^2$ -10$^{8}$ Hz. In particular, dynamics- dependent suppression of peaks turned out to be very sensitive to the motion of 10$^{-4}$ s and 10$^{-5}$ s interfered with frequency of magic angle spinning and proton decoupling, respectively. It is also noteworthy that such dynamic feature is strongly dependent upon the manner of 2D crystalline packing: $^{13}$ C NMR peaks of monomeric bR yielded either highly broadened or completely suppressed signals, depending upon the type of $^{13}$ C-labeled amino-acid residues.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.157-161
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• 1999

This study was performed to enhance the proliferation rate of Gladiolus 'Topaz' callus. The callus was induced from the cermet tissue explants on MS solid medium with 10 mg/L 2,4-D. In the case of liquid shaking culture, proliferation of the callus was effective in MS medium with 0.05 mg/L 2,4-D at 2$0^{\circ}C$ under 16 hours daylength and in a 100 mL Erlenmeyer flask containing 20 mL of the liquid medium and at 75 rpm in rotation speed of the horizontal shaking culture. Furthermore the callus was also able to be subcultured in the same liquid medium.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.952-956
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• 1995

It is known that the 3D Solid CAD system can provide various information which is useful for implementing CAPP and CAE. However the commercial 3D CAD systems available today do not support the handling of non-geometric information such as geometry tolerance and surface finish. It is impossible to input the non-geometric information during designof parts while CAPP needs the information for selecting machine tools. fiztures, inspection method, etc. In this paper the need of research on handling tolerance information In 3D CAD systems is considered. The development of inspection planning support system is also explained with an example. The development of inspection planning support systm receives the design geometry information from the 3D CAD system in the form of 2D draft and generates the inspection data base and the inspection sheet through the user interaction.

• Toxicological Research
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• v.31 no.3
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• pp.255-264
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• 2015

Heterocyclic amines (HCAs) and acrylamide are unintended hazardous substances generated by heating or processing of foods and are known as carcinogenic and mutagenic agents by the animal experiments. A simple method was established for a rapid and accurate determination of 12 types of HCAs (IQ, MeIQ, Glu-P-1, Glu-P-2, MeIQx, Trp-P-1, Trp-P-2, PhIP, $A{\alpha}C$, $MeA{\alpha}C$, Harman and Norharman) and acrylamide in three food matrices (non-fat liquid, non-fat solid and fat solid) by isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS). In every sample, a mixture of internal standards including $IQ-d_3$, $MeIQx-d_3$, $PhIP-d_3$, $Trp-P-2-^{13}C_2-^{15}N$ and $MeA{\alpha}C-d_3$ was spiked for quantification of HCAs and $^{13}C_3$-acrylamide was also spiked for the analysis of acrylamide. HCAs and acrylamide in sample were extracted with acetonitrile and water, respectively, and then two solid-phase extraction cartridges, ChemElut: HLB for HCAs and Accucat: HLB for acrylamide, were used for efficiently removing interferences such as pigment, lipid, polar, nonpolar and ionic compounds. Established method was validated in terms of recovery, accuracy, precision, limit of detection, limit of quantitation, and linearity. This method showed good precision (RSD < 20%), accuracy (71.8~119.1%) and recovery (66.0~118.9%). The detection limits were < 3.1 ng/g for all analytes. The correlation coefficients for all the HCAs and acrylamide were > 0.995, showing excellent linearity. These methods for the detection of HCAs and acrylamide by LC-MS/MS were applied to real samples and were successfully used for quantitative monitoring in the total diet study and this can be applied to risk assessment in various food matrices.

• Composites Research
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• v.28 no.2
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• pp.58-64
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• 2015

The purpose of this paper is to suggest the effective equivalent finite element model for the impact limiter of a nuclear spent fuel shipping cask made of sandwich composite panels. The sandwich composite panels were composed of a metallic facesheet and a core material made of urethane foam, balsa wood and red wood, respectively. The effective equivalent finite element model for the impact limiter was proposed by comparing the results of low-velocity impact test of sandwich panels. An explicit finite element analysis based on LS-DYNA 3D was done in this study. The results showed that the solid elements were recommended to model the facesheet and core of sandwich panels for impact limiter compared to combination modeling method, in which the layered shell element for facesheet and solid element for core material are used. In particular, the solid element for balsa and red wood core materials should be modeled by the element elimination approach.

• Journal of computational fluids engineering
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• v.13 no.3
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• pp.8-13
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• 2008

We present a numerical simulation technique and some preliminary results of the impact and spreading of a droplet containing particles on the solid substrate in 2D. We used the 2nd-order Adams-Bashforth / Crank-Nicholson method to solve the Navier-Stokes equation and employed the level-set method with the continuous surface stress for description of droplet spreading with interfacial tension. The impact velocity has been generated by the instantaneous gravity. The distributed Lagrangian-multipliers method has been combined for the implicit treatment of rigid particles and the discontinuous Galerkin method has been used for the stabilization of the interface advection equation. We investigated the droplet spreading by the inertial force and discussed effects of the presence of particles on the spreading behavior using an example problem. We observed reduced oscillation and spread for the particulate droplet.

• Journal of Electrochemical Science and Technology
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• v.9 no.2
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• pp.126-132
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• 2018

For the first time, an innovative approach using P(VDF-TrFE) as a polymer electrolyte for high efficiency, all-solid-state supercapacitors is presented. The polymer electrolyte was successfully achieved by dissolving P(VDF-TrFE) copolymers in dimethylformamide (DMF). Thermal analysis and infrared spectroscopy revealed excellent thermal stability up to $400^{\circ}C$ and copolymer's interaction with DMF. Electrochemical capacitors fabricated using P(VDF-TrFE) in DMF and ZnO NWs demonstrated high capacitive performance. Furthermore, the gel electrolyte-based supercapacitors demonstrated excellent mechanical durability up to a bend angle of $120^{\circ}$. Novel P(VDF-TrFE) electrolytes could be a promising approach for applications in flexible, fabric-based, and high-efficiency energy devices.

• Research in Mathematical Education
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• v.18 no.2
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• pp.103-128
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• 2014

This paper presents the Global van Hiele (GVH) questionnaire as a tool for mapping knowledge and understanding of plane and solid geometry. The questionnaire facilitates identification of the respondents' mastery of the first three levels of thinking according to van Hiele theory with regard to key geometrical topics. Teacher-educators can apply this questionnaire for checking preliminary knowledge of mathematics teaching candidates or pre-service teachers. Moreover, it can be used when planning a course or granting exemption from studying in basic geometry courses. The questionnaire can also serve high school mathematics teachers who are interested in exposing their students to multiple-choice questions in geometry.

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.2
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• pp.367-375
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• 1990

A data structure representing 3-D objects was designed for the personal computer. It is very simple to be used in the personal computers which have small memory and low speed. A homogeneous transformation for developing 3-D objects was derived. Each developing procedure consists of five transformations : one translational-three rotational-one translational. Developing a solid is a creative work. So the results of developing vary with the order of surfaces to be developed. One method to reduce the length of seam was considered. The programs used in this study were written in Pascal and Assembly and a modeller that generates 3-D primitives was included. This program is an interactive dual-screen system. While all the menus in Korean are displayed at the monochrome monitor, the development figures with projective views are drawn at the color monitor. The program has wide applications for plate works.

• Proceedings of the Korean Institute of Communication Sciences Conference
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• 1991.10a
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• pp.69-72
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• 1991

This paper proposes an efficient algorithm that constructs 3 dimensional solid object from orthographic views. The 3D object construction algorithm inputs vertices and edges information of 3 orthogonal views, generates 2 dimensional surfaces of input views, 3 dimensional possible vertices, possible edges and possible surfaces, compares 2 dimensional projections of 3 dimensional possible surface with two dimensional surfaces from orthogonal views and then determines the solution. This algorithm has been proved to be efficient in reducing the time taken and is useful for CAD system, 3 dimensional scene analysis system and object modellings for 3D graphics. The algorithm has been implemented in C language on the IBM PC/AT.