• Title/Summary/Keyword: 2D solid

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Response of Mulberry Brown Leaf Spot Fungus Myrothecium roridum to Different Plant Extracts

  • Chattopadhyay, S.;Institute, Traning;Majil, M.D.;Pratheesshkumar;Das, K.K.;Saratchandra, B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.2
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    • pp.183-188
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    • 2002
  • Anti-fungal potential of 5 plant extracts viz., Eucalyptus citriodora, Allium sativum, Cassia sophera, Chromolaena odorata and Datura metel on the growth of mulberry brown leaf spot pathogen Myrothecium roridum were examined. Except fur the aqueous extract of Allium bulb, ethanolic leaf extract of all other plants more efficiently reduced the colony growth of the fungus on potato-dextrose-agar, Of which, Allium and Eucalyptus extracts were more effective. Initiation of radial growth of M. roridum on solid media was deferred maximum 6 days by ethanolic Eucalyptus extract and 4 days by aqueous Allium extract at $0.4 mg.ml^{-1}$. In the liquid media amended with Eucalyptus extract ($0.4 mg.ml^{-1}$) complete inhibition of sporulation was noticed upto 8 days, and initial inhibition of mycelial bio-mass generation was considerably diminished with time and reduction was 1.3 fold 14 days after application. While, complete inhibition of mycelial growth for 6-14 days was recorded with $\geq$0.1 mg.ml$^{-1}$ commercial eucalyptus oil. However, rejuvenation of growth appeared when fungus was re-inoculated in fresh media. Post-inoculate application of different doses Of Eucalyptus and Allium extracts significantly (p < 0.05) reduced the disease severity in pot-ted mulberry. However, persistence of the effect up to 28 days was apparent at $\geq$ 1.0 mg.ml$^{-1}$ and effectively was on par with carbendazim (1 mg.ml$^{-1}$ ). Almost equal control ability of 1.0 mg.ml$^{-1}$ Eucalyptus extracts can be achieved by ca. 10 times lowered dose of commercial eucalyptus oil. It seems, the toxic principle of E. citrodora to M. roridum is fungistatic in nature and may have essential oil based origin.

Train-Structure Dynamic Interaction Analysis of The Bridge Transition Considering Track Irregularity (궤도틀림을 고려한 교대접속부의 열차상호동적거동해석)

  • Choi, Chan-Yong;Kim, Hun-Ki;Chung, Keun-Young;Yang, Sang-Beom
    • Journal of the Korean Geotechnical Society
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    • v.31 no.9
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    • pp.29-38
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    • 2015
  • In this study, track dynamic interaction characteristics caused by the vehicle running through transitional section such as bridge abutments were studied using the finite element analysis program. The geometric condition of track was generated by trigonometric function and allowable maximum track irregularity is determined by KORAIL track maintenance criteria. The sub-infrastructure under rail fastener system was modelled by 3D solid elements. To reduce computational cost only half track line is numerically considered and the roller boundary condition was applied to each side of model. In this study, the vehicle-track dynamic interaction analysis was carried out for standard Korean transition section of concrete track and the dynamic behaviors were investigated. The dynamic characteristics considered are wheel load variation, vertical acceleration at body, and maximum Mises stress at each part of transitional section.

Development of Large Superalloy Exhaust Valve Spindle by Dissimilar Inertia Welding Process (이종재료 마찰용접에 의한 초내열합금 대형 배기밸브 스핀들 개발)

  • Park Hee-Cheon;Jeong Ho-Seung;Cho Jong-Rac;Lee Nak-Kyu;Oh Jung-Seok;Han Mvoung-Seoup
    • Journal of Advanced Marine Engineering and Technology
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    • v.29 no.8
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    • pp.891-898
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    • 2005
  • Inertia welding is a solid-state welding process in which butt welds in materials are made in bar and in ring form at the joint race, and energy required lot welding is obtained from a rotating flywheel. The stored energy is converted to frictional heat at the interface under axial load. The quality of the welded joint depends on many parameters, including axial force, initial revolution speed and energy amount of upset. working time, and residual stresses in the joint. Inertia welding was conducted to make the large exhaust valve spindle for low speed marine diesel engine. superalloy Nimonic 80A for valve head of 540mm and high alloy SNCrW for valve stem of 115mm. Due to different material characteristics such as, thermal conductivity and flow stress. on the two sides of the weld interface, modeling is crucial in determining the optimal weld geometry and Parameters. FE simulation was performed by the commercial code DEFORM-2D. A good agreement between the Predicted and actual welded shape is observed. It is expected that modeling will significantly reduce the number of experimental trials needed to determine the weld parameters. especially for welds for which are very expensive materials or large shaft. Many kinds of tests, including macro and microstructure observation, chemical composition tensile , hardness and fatigue test , are conducted to evaluate the qualify of welded joints. Based on the results of the tests it can be concluded that the inertia welding joints of the superalloy exhaust valve spindle are better properties than the material specification of SNCrW.

A comparison of fixation methods using three-dimensional finite element analysis following anterior segmental osteotomy

  • Yun, Kyoung In;Park, Min-Kyu;Park, Myung-Kyun;Park, Je Uk
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.38 no.6
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    • pp.332-336
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    • 2012
  • Objectives: This study sought to evaluate fixation methods and determine the best method for the postoperative stabilization of maxillary osteotomy. For our analysis we performed a three-dimensional finite element analysis of stress distribution on the plate, screw, and surrounding bone, as well as displacement onto the plate. Materials and Methods: We generated a model using synthetic skull scan data; an initital surface model was changed to a solid model using software. Modified anterior segmental osteotomy (using Park's method) was made using the program, and four different types of fixation methods were used. An anterior load of 100 N was applied on the palatal surface of two central incisors. Results: The Type 1 (L-shaped) fixation method gave stresses of 187.8 MPa at the plate, 45.8 MPa at the screw, and 15.4 MPa at the bone around the plate. The Type 2 (I-shaped) fixation method gave stresses of 186.6 MPa at the plate, 75.7 MPa at the screw, and 13.8 MPa at the bone around the plate. The Type 3 (inverted L-shaped) fixation method gave stresses of 28.6 MPa at the plate, 29.9 MPa at the screw, and 15.3 MPa at the bone around the plate. The Type 4 (I-shaped) fixation method gave stresses of 34.8 MPa at the plate, 36.9 MPa at the screw, and 14.9 MPa at the bone around the plate. The deflection of the plates for the four fixation methods was 0.014 mm, 0.022 mm, 0.017 mm, and 0.018 mm, respectively. Conclusion: The Type 3 (inverted L-shaped) fixation method offers more stability than the other fixation methods. We therefore recommend this method for the postoperative stabilization of maxillary osteotomy.

Slip Behavior of High-Tension Bolted Joints Subjected to Compression Force (압축력을 받는 고장력 볼트 이음부의 미끄러짐 거동)

  • Han, Jin Hee;Choi, Jong Kyoung;Heo, In Sung;Kim, Sung Bo
    • Journal of Korean Society of Steel Construction
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    • v.20 no.2
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    • pp.279-288
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    • 2008
  • In this study, the slip behavior of high-tension bolted joints subjected to compression force is investigated through 3D finite element analysis and experiments. The relation with sliding load, bolt deformation, and failure load are studied with the metal thickness affecting the bolted joint. The post-sliding behavior considering bolt stiffness is presented and compared with the results by finite element and experiments. The finite element model is constructed by solid elements in ABAQUS, in consideration of all the friction effects between metal plates and bolts. The stress-strain relations in the literature are used, and the sliding displacements and axial stresses around the bolt connection are investigated. The flexural buckling of species happened when the plate thickness is less than the bolt diameter. However, the shear failures of bolt occurred in the opposite case.

Stabilization of Retinol through Incorporation into Liposomes

  • 이승철;육현균;이동훈;이경은;황용일;Richard D. Ludescher
    • BMB Reports
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    • v.35 no.4
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    • pp.358-363
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    • 2002
  • Chemical and photochemical processes during storage and preparation rapidly degrade retinol, the most active form of vitamin A. therefore, the efficacy of incorporation into liposomes in order to modulate the kinetics of retinol degradation was investigated. Retinol was readily incorporated into multilamellar liposomes that were prepared form soybean phosphatidylcholine; the extent of the incorporation was 98.14±0.93% at pH 9.0 at a ratio of 0.01 : 1 (wt:wt) retinol : phospholipid. It was only marginally lower at higher retinol concentrations. The pH of the hydration buffer had a small effect. The incorporation efficiency ranged from 99.25±0.47% at pH 3 to 97.45±1.13% at pH 11. The time course of the retinol degradation in the aqueous solution in liposomes was compared to that of free retinol and free retinol with α-tocopherol under a variety of conditions of pH(3, 7, and 11), temperature(4, 25, 37, and 50℃), and light exposure(dark, visible, and UV). The retinol that was incorporated into the liposomes degraded significantly slower than the free retinol or retinol with α-tocopherol at pH 7 and 11. At pH 3, where the free retinol degrades rapidly, the degradation kinetics were similar in liposomes and the presence of α-tocopherol. At pH 7.0 and 4℃ in the light, for example, free aqueous retinol was completely degraded within 2 days, while only 20% of the retinol in the liposomes were degraded after 8 days. In general, the protective effect of the liposome incorporation was greater at low temperatures, at neutral and high pH, and in the dark. The results suggest that protection is greater in the solid, gel phase than in the fluid liquid crystalline phase lipids. These results indicate that the incorporation into liposomes can extend the shelf-life of retinol under a variety of conditions of temperature, pH, and ambient light conditions.

Synthesis and base pairing properties of DNA-RNA heteroduplex containing 5-hydroxyuridine

  • Cui, Song;Kim, Yong-Hoon;Jin, Cheng-Hao;Kim, Sang-Kook;Rhee, Man-hee;Kwon, Oh-Shin;Moon, Byung-Jo
    • BMB Reports
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    • v.42 no.6
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    • pp.373-379
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    • 2009
  • 5-Hydroxyuridine (5-OHU) is a major lesion of uridine and cytosine produced in RNA by various chemical oxidants. To elucidate its biochemical and biophysical effects on RNA replication, the site-specifically modified oligoribonucleotides containing 5-OHU were synthesized with C5-hydroxy-5'-ODMTr-2'-TBDMS-uridine phosphoramidite using automated solid phase synthesis. The base-pairing properties of nucleotides opposite 5-OHU in 24 mer oligoribonulcleotides with dNTP were studied using three reverse transcriptases (Super-$Script^{TM}II$-, AMV-, MMLV-RT) in cDNA synthesis. Adenine as well as guanine was incorporated preferentially by all reverse transcriptases. In the UV-melting temperature experiment, the results from the relative stabilities of the base pairs were A : 5-OHU > G : 5-OHU > T : 5-OHU $\approx$ C : 5-OHU. Circular Dichroism (CD) studies showed that DNA-RNA containing 5- OHU heteroduplexes exhibit a similar conformation between the A-type RNA and B-type DNA. These results suggest that 5- OHU from oxidative damage was mainly influenced by adenine mismatch.

Methane hydrate : The state of the art of Production technologies and environmental issues (메탄 하이드레이트의 생산 기술 현황과 환경에 미치는 영향)

  • Chang Seung yong
    • The Korean Journal of Petroleum Geology
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    • v.7 no.1_2 s.8
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    • pp.13-18
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    • 1999
  • Methane hydrate is an ice-like solid material and it has a structure which water molecules enclose gas molecules. For low temperature and high pressure, hydrocarbon gas forms hydrate and due to this condition, it is existed in the arctic region or deep sea. Presently, the amount of methane hydrate is unpredictable, but it is assumed that the amount will be enormous. For this reason, it is expected that it will play a major role as natural gas resources in the future. However, the production technologies are stayed on the low level and the economical technology was not developed yet. Also, emission of natural gas from methane hydrate will cause global warming and thus it is considered as a critical environmental problem. In this paper, the state of the art of the production technologies and environmental effects of methane hydrate were summarized.

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Studies on Moisture Evaporation and Economical Analysis for Composting of Piggery Slurry (양돈 슬러리 퇴비화 방법의 수분증발율 및 경제성 분석에 관한 연구)

  • Park, C.H.;Yoon, T.H.;Ryoo, J.W.;Kim, J.H.;Choi, T.B.;Choi, D.Y.;Kwag, J.H.;Jeong, K.W.;Kim, H.H.
    • Journal of Animal Environmental Science
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    • v.8 no.2
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    • pp.119-124
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    • 2002
  • To evaluate composting process of piggery slurry, we made $1m^3$ bench scale composting reactor, and investigated the input and output of mass, moisture evaporation, volatile solid degradation, and running cost. For 15 days experimentation the results was as followed, The quantity of sawdust used was 0.47ton(18,000won) per 1ton slurry. The amount of moisture evaporated was 16.8kg/kg VS degradated. Total evaporation and VS degradation were 24.8%, 5.7% respectably. To analysis the running cost of that, we included only two factors, sawdust cost and compost income. The compost price to sell was 63won/kg(50% of market price) it made 8,694won income to 756 won loss, but 25won/kg, it was $13,650{\sim}23,100won$ loss per 1 ton slurry in the current pig farmer's condition. This cost was $8.5{\sim}14.4%$ of pig production cost and damage strongly to pig farmers to treat it.

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Effects of Psychrotrophic Bacteria, Serratia liquefaciens and Acinetobacter genomospecies 10 on Yogurt Quality

  • Shin, Yong Kook;Oh, Nam Su;Lee, Hyun Ah;Choi, Jong-Woo;Nam, Myoung Soo
    • Food Science of Animal Resources
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    • v.34 no.4
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    • pp.543-551
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    • 2014
  • The aim of this study was to evaluate the effect of proteolytic (Serratia liquefaciens, match %: 99.39) or lipolytic (Acinetobacter genomospecies 10, match %: 99.90) psychrotrophic bacteria (bacterial counts, analysis of free fatty acids (FFA) and analysis of free amino acids) on the microbial and chemical properties (yogurt composition), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of yogurt during storage. Yogurts were prepared with raw milk preinoculated with each psychrotrophic bacteria. The total solid, fat, and protein content were not affected by preinoculation, but the pH of yogurt preinoculated with psychrotrophic bacteria was higher than in control. There was a dramatic increase in short chain free fatty acids among FFA in yogurt with Acinetobacter genomospecies 10. For 14 d of cold storage condition, SCFFA was 25.3 mg/kg to 34.4 mg/kg (1.36 times increased), MCFFA was 20.4 mg/kg to 25.7 mg/kg (1.26 times increased), and LCFFA was 240.2 mg/kg to 322.8 mg/kg (1.34 times increased). Serratia liquefaciens (match %: 99.39) in yogurt caused a greater accumulation of free amino acids (FAA), especially bitter peptides such as leucine, valine, arginine, and tyrosine, but SDS-PAGE showed that the inoculation of Serratia liquefaciens did not affect the degree of casein degradation during storage. Taken together, the excessive peptides and FFA in yogurt generated from psychrotrophic bacteria could develop off-flavors that degrade the quality of commercial yogurt products.