• Journal of the Korean Association of Geographic Information Studies
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• v.25 no.4
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• pp.136-150
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• 2022

In this study, a comparative review was conducted on how to use RGB images to obtain river topographic information, which is one of the most essential data for eco-friendly river management and flood level analysis. In terms of the topographic information of river zone, to obtain the topographic information of flow section is one of the difficult topic, therefore, this study focused on estimating the river topographic information of flow section through RGB images. For this study, the river topography surveying was directly conducted using ADCP and RTK-GPS, and at the same time, and orthomosiac image were created using high-resolution images obtained by drone photography. And then, the existing developed regression equations were applied to the result of channel topography surveying by ADCP and the band values of the RGB images, and the channel bathymetry in the study area was estimated using the regression equation that showed the best predictability. In addition, CCHE2D flow modeling was simulated to perform comparative verification of the topographical informations. The modeling result with the image-based topographical information provided better water depth and current velocity simulation results, when it compared to the directly measured topographical information for which measurement of the sub-section was not performed. It is concluded that river topographic information could be obtained from RGB images, and if additional research was conducted, it could be used as a method of obtaining efficient river topographic information for river management.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2000.04a
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• pp.9-10
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• 2000

An important business-field of world-wide steel-industry is the coating of thin metal-sheets with zinc, zinc-aluminum and aluminum based materials. These products mostly go into automotive industry. in particular for the car-body. into building and construction industry as well as household appliances. Due to mass-production, the processing is done in large continuously operating plants where the mostly cold-rolled metal-strip as the substrate is handled in coils up to 40 tons unwind before and rolled up again after passing the processing plant which includes cleaning, annealing, hot-dip galvanizing / aluminizing and chemical treatment. In the liquid Zn, Zn-AI, AI-Zn and AI-Si bathes a combined action of corrosion and wear under high temperature and high stress onto the transfer components (rolls) accounts for major economic losses. Most critical here are the bearing systems of these rolls operating in the liquid system. Rolls in liquid system can not be avoided as they are needed to transfer the steel-strip into and out of the crucible. Since several years, ceramic roller bearings are tested here [1.2], however, in particular due to uncontrollable Slag-impurities within the hot bath [3], slide bearings are still expected to be of a higher potential [4]. The today's state of the art is the application of slide bearings based on Stellite\ulcorneragainst Stellite which is in general a 50-60 wt% Co-matrix with incorporated Cr- and W-carbides and other composites. Indeed Stellite is used as the bearing-material as of it's chemical properties (does not go into solution), the physical properties in particular with poor lubricating properties are not satisfying at all. To increase the Sliding behavior in the bearing system, about 0.15-0.2 wt% of lead has been added into the hot-bath in the past. Due to environmental regulations. this had to be reduced dramatically_ This together with the heavily increasing production rates expressed by increased velocity of the substrate-steel-band up to 200 m/min and increased tractate power up to 10 tons in modern plants. leads to life times of the bearings of a few up to several days only. To improve this situation. the Mechanical Alloying (MA) TeChnique [5.6.7.8] is used to prOduce advanced Stellite-based bearing materials. A lubricating phase is introduced into Stellite-powder-material by MA, the composite-powder-particles are coated by High Energy Milling (HEM) in order to produce bearing-bushes of approximately 12 kg by Sintering, Liquid Phase Sintering (LPS) and Hot Isostatic Pressing (HIP). The chemical and physical behavior of samples as well as the bearing systems in the hot galvanizing / aluminizing plant are discussed. DependenCies like lubricant material and composite, LPS-binder and composite, particle shape and PM-route with respect to achievable density. (temperature--) shock-reSistibility and corrosive-wear behavior will be described. The materials are characterized by particle size analysis (laser diffraction), scanning electron microscopy and X-ray diffraction. corrosive-wear behavior is determined using a special cylinder-in-bush apparatus (CIBA) as well as field-test in real production condition. Part I of this work describes the initial testing phase where different sample materials are produced, characterized, consolidated and tested in the CIBA under a common AI-Zn-system. The results are discussed and the material-system for the large components to be produced for the field test in real production condition is decided. Outlook: Part II of this work will describe the field test in a hot-dip-galvanizing/aluminizing plant of the mechanically alloyed bearing bushes under aluminum-rich liquid metal. Alter testing, the bushes will be characterized and obtained results with respect to wear. expected lifetime, surface roughness and infiltration will be discussed. Part III of this project will describe a second initial testing phase where the won results of part 1+11 will be transferred to the AI-Si system. Part IV of this project will describe the field test in a hot-dip-aluminizing plant of the mechanically alloyed bearing bushes under aluminum liquid metal. After testing. the bushes will be characterized and obtained results with respect to wear. expected lifetime, surface roughness and infiltration will be discussed.

• Food Science and Biotechnology
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• v.14 no.4
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• pp.503-508
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• 2005

Screening for antilisterial activity was performed in about three thousand isolates of lactic acid bacteria (LAB) from Chinese cabbage kimchi, and finally based on the relatively stronger antilisterial activities eight bacterial strains were selected. The bacteria were further characterized in terms of their tolerance to artificial gastric juice, pH 2.5, bile salts (0.3% oxgall), and to the different NaCl concentrations. Of the isolates, YK005 was especially investigated for its physiological characteristics due to its inhibitory activity against gram-positive Listeria monocytogenes as well as gram-negative Escherichia coli O157:H7, as they have been constantly reported to be resistant against bacteriocins produced by a number of LAB strains. YK005 was found to be rod-shaped, $3.8\;{\mu}m$ long ${\times}\;0.5\;{\mu}m$ wide, non-sporeforming, non-motile, catalase-negative, and produced gas from glucose (heterolactic). Based on the biochemical data obtained by API 50 CHL medium, the isolate was tentatively identified as Lactobacillus brevis. To validate the result obtained by the biochemical identification, rRNA-based PCR experiments using a pair of species-specific primers for L. brevis were conducted and a single band of 1400 bp was observed, which strongly indicated that YK005 belongs to L. brevis. The LAB isolates are potentially exploited as human probiotic organisms and are employed to control some food-borne pathogens like L. monocytogenes.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.6
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• pp.739-747
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• 2000

This experiment was conducted to evaluate the influence of dimethyl-sulfoxise (DMSO, 0, 5, 50, 100 and $500{\mu}M$) on the motility and acrosome reaction of the frozen-thawed spermatozoa from 3 different bulls (Bull A, Band C). Also we evaluated the developmental capacity of bovine embryos fertilized in a medium containing DMSO at various concentrations. DMSO had negligible effects on the sperm motility and acrosome reaction in all three bulls. However, the development rates from 2 to 16 cells stage on the 3rd day after insemination with 50, 100 and $500{\mu}M$ DMSO in Bull-B, and up to the blastocyst stage fertilized with 5, 50, 100 and $500{\mu}M$ in Bull-A were significantly higher (p<0.05) than those of control ($0{\mu}M$ DMSO) group from each bull. Furthermore, the rates of blastocysts per cleaved embryos of 5 to $500{\mu}M$ DMSO group in Bull-A and of 5 to $100{\mu}M$ DMSO in Bull-C were also significantly higher (p<0.05) than those for their $0{\mu}M$ groups, respectively. These results indicate that DMSO at micromol level used for in vitro fertilization might stimulate the development of embryos for some bulls.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.1648-1653
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• 2003

The paper studies effect of quasi-periodic or airplane flutter phenomenon on television broadcasting signal. Airplane flutter is a very important problem. It causes the receiving antenna to receive both direct signal by the Tx (Transmitter antenna) and reflected signal scattered by the airplane with phase delay. The sum of two signals results in fading, sometime collapse and distortion of picture on TV screen. We performed measurement and modeling this phenomenon on TV signal when the airplane flew across and range Tx and Rx (Receiver antenna). The frequency 60.75MHz (Aural frequency of CH3) is used under tests. A single scatter multipath model is introduced. It is used to duplicate some of the measured data and show the dependence of power variation on the airplane fluttering. The fluctuation of the airplane flutter phenomenon was calculated to be around 2-4dB. The Yaki antenna is used for improving airplane flutter problem because it can make high gain and high directivity.

• Journal of electromagnetic engineering and science
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• v.17 no.3
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• pp.153-158
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• 2017

This work reports on the effect of plasma area on the frequency characteristics of the monostatic radar cross section (RCS) of a square metallic plate. A dielectric barrier discharge (DBD) plasma actuator consisting of 10 rings is proposed. The actuator is fabricated in three different configurations such that only three inner rings, seven inner rings, and all rings can be biased. By applying an 18-kV bias at 1 kHz, the three types of DBD actuators generate plasma with a total area of 16.96, 36.74, and $53.69cm^2$, respectively, in a ring or circular form. The experimental results reveal that when the DBD actuator is placed in front of a $20mm{\times}20cm$ conducting plate, the monostatic RCS is reduced by as much as 18.5 dB in the range of 9.41-11.65 GHz. Furthermore, by generating the plasma and changing the area, the frequency of maximum reduction in the monostatic RCS of the plate can be controlled. The frequency is reduced by nearly 20% in the X band when all rings are biased. Finally, an electromagnetic model of the plasma is obtained by comparing the experimental and full-wave simulated results.

• Biomedical Science Letters
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• v.13 no.2
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• pp.105-110
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• 2007

Aspartyl-tRNA synthetase (AspRS) exists in two different forms with respect to tRNA recognition. The discriminating enzyme (D-AspRS) recognizes only tRNA$^{Asp}$, while the non-discriminating one (ND-AspRS) also recognizes tRNA$^{Asn}$ and therefore forms both Asp-tRNA$^{Asn}$ and Asp-tRNA$^{Asp}$. Plus primary sequence distinguishes two general groups of AspRS. There is a predominantly bacterial-type, larger AspRS (about 580 aa) in addition to a shorter archaeal/eukaryotic type (about 430 aa). In vivo data made clear that discriminating and non-discriminating enzymes exist in both groups. The determinants in the protein sequence responsible for tRNA discrimination are not hewn. The AspRS from Acidithiobacillus ferrooxidans might be suggested ND-AspRS fur missing of AsnRS in genomic sequencing data. Therefore, we analyzed the AspRS from A. ferrooxidans with in vitro aminoacylation assay with E. coli unfractionated tRNA, in vivo missense suppression assay with tipA34 mutant and Northern hybridization with probes which were specific with tRNA$^{Asp}$ or tRNA$^{Asn}$. The AspRS from A. ferrooxidans produced more Asp-tRNA than that from E. coli. Only aspS gene from A. ferrooxidans suppressed trpA34 strain in minimal media without tryptophan. Only AspRS from A. ferrooxidans showed mischarged Asp-tRNA$^{Asn}$ band. Therefore, AspRS from A. ferrooxidans is definitely ND-AspRS.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.538-541
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• 2005

Tidal flats develop along the south coast ofthe Korean peninsula. These areas are famous for sea farming. Specially, strong and coherent radar backscattering signals are observed over oyster sea farms that consist of artificial structures. Tide height in oyster farm is possible to measure by using interferometric phase and intensity of SAR data. It is assumed that the radar signals from oyster farm could be considered as double-bouncing returns by vertical and horizontal bars. But, detailed backscattering mechanism and polarimetric characteristics in oyster farm had not been well studied. We could not demonstrate whether the assumption is correct or not and exactly understand what the properties of back scattering were in oyster farm without full polarimetric data. The results of AIRSAR L-band POLSAR data, experiments in laboratory and JERS-l images are discussed. We carried out an experiment simulating a target structure using vector network analyser (Y.N.A.) in an anechoic chamber at Niigata University. Radar returns from vertical poles are stronger than those from horizontal poles by 10.5 dB. Single bounce components were as strong as double bounce components and more sensitive to antenna look direction. Double bounce components show quasi-linear relation with height of vertical poles. As black absorber replaced AI-plate in bottom surface, double bounce in vertical pole decreased. It is observed that not all oyster farms are characterized by double bounced scattering in AIRSAR data. The image intensity of the double bounce dominant oyster farm was investigated with respect to that of oyster farm dominated by single bounce in JERS-l SAR data. The image intensity model results in a correlation coefficient (R2 ) of 0.78 in double bounce dominant area while that of 0.54 in single bouncing dominant area. This shows that double bounce dominant area should be selected for water height measurement using In8AR technique.

• BMB Reports
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• v.32 no.5
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• pp.461-467
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• 1999

Recombinant Mycobacterium smegmatis expressing ovalbumin was used to immunize C57BL/6(H-$2^b$) mice, and the humoral immunity against recombinant ovalbumin was analyzed. Antibodies were purified by denatured ovalbumin-conjugated affinity chromatography. The epitopes of the antibodies were screened with a random peptide library displayed on the tip of fUSE5 filamentous phage pIII minor coat proteins. Two peptides, IRLADR and SPGAEV, were selected predominantly by the recognition of purified antibodies using biopanning methods. The composition of the peptide sequence with the primary structure of OVA revealed that the peptide sequence analogizes to INEAGR, part of the $^{323}ISQAVHAAHAEINEAGR^{339}$ sequence previously reported as the antigenic determinant for murine Band also Th cell epitopes (I-$A^d$ binding). Also, the structures of these mimotopes obtained from restrained molecular dynamic computations resulted in the formation of a $\beta$-turn proven to be a secondary structure of the parent peptide within the ovalbumin molecule, enabling us to confirm the structural similarity. This study demonstrates that immunization with recombinant M. smegmatis can generate neutralizing antibodies identical with those induced by the administration of natural antigenic proteins and supports the potential use of mycobacteria as vaccine delivery vehicles.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.11
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• pp.1950-1956
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• 1997

An engineering goal in vibration and noise professionals is to develope quiet machines at the preliminary design stage, and various numerical techniques such as FEM, SEA or BEM are one of the schemes toward the goal. In this paper, the research has been focused on the sensitivity effect of mesh sizes for FEM application so that the optimum size of the mesh that leads to engineering solution within acceptable computing time could be generated. In order to evaluate the mesh size effect, three important parameters have been examined : natural frequencies, number of modes and driving point mobility. First, several lower modes including the fundamental frequency of a 2-D plate structure have been calculated as mesh size changes. Since theoretical values of natural frequencies for a simple structure are known, the deviation between the numerical and theoretical values is obtained as a function of mesh size. The result shows that the error is no longer decreased if the mesh size becomes a quarter wavelength or smaller than that. Second, the mesh size effect is also investigated for the number of modes. For the frequency band up to 1.4 kHz, the structure should have 38 modes in total. As the mesh size reaches to the quarter wavelength, the total count in modes approaches to the same values. Third, a mobility function at the driving point is compared between SEA and FEM result. In SEA application, the mobility function is determined by the modal density and the mass of the structure. It is independent of excitation frequencies. When the mobility function is calculated from a wavelength to one-tenth of it, the mobility becomes constant if the mesh becomes a quarter wavelength or smaller. We can conclude that dynamic parameters, such as eigenvalues, mode count, and mobility function, can be correctly estimated, while saving the computing burden, if a quarter wavelength (.lambda./4) mesh is used. Therefore, (.lambda./4) mesh is recommended in structural vibration analysis.