• Bulletin of the Korean Chemical Society
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• v.14 no.5
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• pp.588-592
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• 1993

The $^1H-NMR$ signals of the heme methyl, propionate and related chemical groups of cytochrome $c_3$ from Desulfovibrio vulgaris Miyazaki F (D.v. MF) were site-specifically assigned by means of 1D-NOE, 2D-DQFCOSY and 2D-TOCSY spectra. They were consistent with the site-specific assignments of the hemes with the highest and second-lowest redox potentials reported by Fan et al. (Biochemistry, 29, 2257-2263 1990). The site-specific heme assignments were also supported by NOE between the methyl groups of these hemes and the side chain of Val-18.

• Applied Biological Chemistry
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• v.42 no.4
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• pp.351-355
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• 1999

In order to find anti-dementia drugs from natural products, prolyl endopeptidase inhibitors were purified from Puerariae Flos by consecutive solvent partition, followed by silica gel, Sephadex LH-20, and HPLC. Four isoflavonoid inhibitors were isolated and identified as tectorigenin, genistein, 5,7-dihydroxy-4',6-dimethoxyisoflavone, and 5-hydroxy-6,7,4'-trimethoxyisoflavone by means of instrumental analyses including $^{1}H-$, $^{13}C-$, $^{2}D-NMR$ and MS and $IC_{50}$ values against PEP were 5.30 ppm$(17.7\;{\mu}M)$, 10.39 ppm$(38.5\;{\mu}M)$, 13.92 ppm$(44.3\;{\mu}M)$, and 20.61 ppm$(62.8\;{\mu}M)$, respectively. Some previous mistakes in $^{13}C-NMR$ assignment were revised by careful investigation of HMBC and HMQC data.

• Natural Product Sciences
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• v.9 no.2
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• pp.52-55
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• 2003

The aerial parts of Ephedra aphylla afforded two alkaloids belonging to two different classes. The first alkaloid, ephedradine C, belonging to the spermine alkaloid, a group characterized by its hypotensive effect. The other alkaloid, hordenine is a phenylalkylamine alkaloid isolated for the first time from Ephedra Species. The structures were elucidated by spectroscopic methods and the assignment of some carbons in ephedradine C was achieved based on 2 D-NMR experiments.

• Fashion & Textile Research Journal
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• v.14 no.4
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• pp.597-606
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• 2012

To increase the productivity and product quality of customized baseball jerseys, this study developed a multi-variable system for a production process that efficiently controls diverse production management factors. The working time was measured through the establishment of a standard process where skilled workers and Chinese factory workers manufactured 5 sets of the same basic design jerseys. Based on the measured working time (1,136 seconds/per unit), the multi-variable process control system was developed, where hourly production management is possible according to the involved workers and equipment types. Each process was assigned accoding to the production management factors for a total of 28 standard processes. The processes were developed based on consideration of work characteristics according to the order of needlework of open-type set baseball jerseys with sleeves(the basic design of baseball jerseys)to result in a customized production system structure that could be set up with multi-variables. As a result, a total 12 types of systems were developed in consideration of the personnel involved and the number of equipments. The optimal production management system (with the highest efficiency compared to the number of workers)was A-2, B-1, C-1. D-2, E-2, F-1, and G-1. This system had extremely high efficiency and showed 99% assignment efficiency for the 7-person team. Though not optimal, possible process assignment for each working personnel is proposed as a reserve process in case work modification is inevitable due to malfunctions and the absence of equipments.

• Journal of the Korean Chemical Society
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• v.47 no.1
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• pp.43-46
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• 2003

From Portulaca oleracea which is widely distributed in Korea and has long been used as a folk medicine, two biophenolic glycosides, 3-hydroxy-1-(2-hydroxyethyl)phenyl-4-O-${\beta}$-D-glucopyranoside (1) and 2-(3,4-dihydroxyphenyl) ethyl-O-${\beta}$-D-glucopyranoside (2) were isolated using column chromatography and reversed-phase HPLC. $^{13}C$ NMR spectral assignment for these compounds was revised by the extensive 2-D NMR experiments such as NOESY, HMQC, and HMBC. These compounds showed a considerable antioxidant effect in DPPH assay system.

• Journal of Korean Society of Transportation
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• v.19 no.2
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• pp.43-52
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• 2001

대부분의 관측교통량기반 수요추정기법은 소규모 및 중규모 교통망 등의 상대적으로 규모가 작은 교통망에서 기본적으로 가정된 수요를 가지고 얻은 추정O/D를 기본O/D와 비교하여 그 추정의 정확성이 어느 정도인가를 오차분석법 등을 이용하여 비교.분석하는 것이 그 주요한 분석방향이라고 할 수 있었다. 이러한 접근법은 실제 현실에서는 알 수 없는 참O/D나 참관측교통량을 가정하고 제시된 모형을 면밀히 관찰하여 모형의 장단점이 무엇인지를 파악하거나 타모형과의 비교.분석을 용이하게 하고자 할 때 많이 이용된다. 그러나 이러한 가정된 교통망이나 참O/D(true O/D) 등은 모형의 적용가능성을 살필 경우에 이용 가능한 방법이라고 할 수 있지만, 참O/D를 알지 못하는 현실상황(대규모 교통망)에서는 추정O/D의 신뢰성을 평가하기란 매우 힘든 작업이거나 거의 불가능한 일이라 할 수 있다. 이러한 문제점을 보완하고자 본 연구에서는 서울시의 1996년도 교통센서스 자료를 이용하여 가정된 수요가 아닌 실제적이고 현실적인 자료를 가지고 대규모 교통망에서 이용될 수 있는 모형을 살펴보았다. 연구방법은 대규모 교통망에 기존의 단일차종기반모형과 본 연구에서 제시한 다차종(multiclass)기반모형을 적용하여 추정된 O/D에 TLFD(Trip Length Frequency Distribution)개념을 이용하여 추정된 O/D의 신뢰성을 평가하고자 하였다. 또한, $R^2$를 이용하여 모형 적용 전후의 관측교통량과 배분교통량을 비교하여 추정력을 분석하였다. 본 연구에서는 단일차종기반모형보다는 차종간 혼잡효과 및 노선선택비율을 차종별로 감안할 수 있는 다차종기반모형이 대규모교통망에서는 보다 적절한 결과를 나타내는 것으로 분석되었다.

• Natural Product Sciences
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• v.16 no.1
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• pp.6-9
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• 2010

In continuing our search for biologically active compounds from Korean Compositae medicinal plants, we investigated the constituents of the aerial parts of Lactuca indica L. and isolated a phenylpropanoid derivative from its MeOH extract. The chemical structure was characterized by spectroscopic methods, including 1D and 2D NMR to be di-E-caffeoyl-meso-tartaric acid (1). Compound 1 was isolated for the first time from this plant. In this paper, we suggest that the NMR assignment at C-2 of (+)-taraxafolin-B should be corrected. In the human HBV-transfected liver cell line HepG2.2.15, the compound 1 effectively reduced HBV DNA level in the release of mature HBV particles from HepG2.2.15 cultivation.

• Korean Journal of Mathematics
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• v.23 no.4
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• pp.607-618
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• 2015

An L(2; 1)-labeling of a graph G is a function f from the vertex set V (G) to the set of all non-negative integers such that ${\mid}f(u)-f(v){\mid}{\geq}2$ if d(u, v) = 1 and ${\mid}f(u)-f(v){\mid}{\geq}1$ if d(u, v) = 2. The ${\lambda}$-number of G, denoted ${\lambda}(G)$, is the smallest number k such that G admits an L(2, 1)-labeling with $k=\max\{f(u){\mid}u{\in}V(G)\}$. In this paper, we consider the square of a cycle and provide exact value for its ${\lambda}$-number. In addition, we also completely determine its edge span.

• Korean Journal of Pharmacognosy
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• v.28 no.2
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• pp.88-92
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• 1997

The full NMR assignment of hispidulin 7-0-neohesperidoside (1) isolated from Cirsium japonicum var. ussuriense was made with the aid of 2D correlation NMR techniques such as HMQC and HMBC. To investigate detoxification of bromobenzene-induced hepatic lipid peroxidation by compound 1, hepatic lipid peroxide level and the activities of enzymes responsible for production and removal of epoxide were studied. The level of lipid peroxide elevated by bromobenzene was significantly reduced by compound 1. This compound administered daily over one week before intoxication with bromobenzene did not affect the activities of aminopyrine N-demethylase, aniline hydroxylase, glutathione S-transferase. Epoxide hydrolase activity was decreased significantly by bromobenzene, which was restored to the control level by pretreatment of persicarin. The results suggest that the bromobenzene-induced hepatic lipid peroxidation by compound 1 is reduced by enhancing the activity of epoxide hydrolase, an enzyme removing bromobenzene epoxide.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.59-66
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• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.