• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.44 no.2
• /
• pp.167-172
• /
• 2011

A three-dimensional ecological model (EMT-3D) was applied to Tokyo Bay to simulate 4,4'-bis (2-sulfostyryl)biphenyl (DSBP). The simulated results were in good agreement with the observed values, with a correlation coefficient of R=0.8431 and a coefficient of determination of $R^2$=0.7108. The sensitivity analysis indicated that the photolysis rate is the most important factor. Therefore, the parameters must be considered carefully in modeling. The mass balance results showed that the standing stock of DSBP in water and in particulate organic carbon was 621.2 and 19.5 kg, respectively, and the effluent flux to the open sea was 2.63 and 0.055 kg/day, respectively.

• Korean Journal of Clinical Laboratory Science
• /
• v.40 no.2
• /
• pp.98-105
• /
• 2008

Colorectal carcinoma from various cancers is fourth ranked occurred to Korean. Due to western dietary life, this cancer has been increased continuously. Therefore, the further study will be needed to find a candidate gene involved in the development and progression of colorectal carcinoma as well as to diagnose and treatment helpfully. The purpose of this study was designed to find a carcinogenesis gene using microsatellite marker on chromosomes 7th and 20th from 30 colon cancer patients. The amplification was investigated in order of D20S97 57% (17/30), D20S101 57% (17/30), D20S119 53% (16/30), D7S483 50% (15/30), D7S495 47% (14/30), D7S498 47% (14/30). The genetic mutation pattern depends on loci of colorectal carcinoma was shown highly amplified with 3.77 from colon cancer than with 2.08 from right colorectal carcinoma (P<0.018). The genetic mutation with lymph nodes was investigated higher with 4.13 at metastasized group than with 1.93 at non-metastasized group (P<0.001). There was no difference at comparison between histological classfication and serological CEA increase as well as on genetic mutated pattern depends on disease stage. It is suggested that the amplification on chromosomes 7q and 20q determines a pivotal role from first stage to metastasis cancer and also functions as an useful marker on diagnosis and treatment of colorectal carcinoma patients as well as follow-up checkup. Recently, the diagnosis and study using genetic analyzer are necessary for efficient application. Fortunately, several university hospitals run this genetic analyzer currently so it is expected that this method makes full use of clinical application.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.2
• /
• pp.173-180
• /
• 2011

Osteocalcin (OC), a marker of bone turnover, displays patterns in relation to physiological and genetic factors. Here, we present an association study in a population of Chinese Holstein cattle (n = 24) with OC serum concentration as a phenotypic trait. We hypothesised that OC status is associated with phylogeny, vitamin D serum level and single nucleotide polymorphisms (SNPs). Mitochondrial DNA (mtDNA) was used as an unlinked marker to examine phylogeny and linkage to measured phenotypic traits of vitamin D and OC status. Following an association study with OC serum variability as the trait, genotyping of SNPs (n = 27) in OC-related genes was performed. Candidate SNPs were chosen in genes with an emphasis on the vitamin D and vitamin K pathways. Multivariant factor analysis revealed a correlation between vitamin D serum concentration and a SNP in the gene GC (rs43338565), which encodes a vitamin D-binding protein, as well as between a SNP in NFATc1 (rs42038422) and OC concentration. However, univariate analysis revealed that population structure, vitamin D serum levels and SNPs were not significant determinants of OC status in the studied group.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2000.11a
• /
• pp.59-75
• /
• 2000

This study was carried out to develop a DNA marker for identifying between Korean cattle (Hanwoo) and other breeds. First experiment was performed to isolate Hanwoo specific DNA marker at sequence characterized amplified regions (SCARs). Five breeds of cattle including Hanwoo, Holstein, Hereford, Angus and Charolais were represented with the from 8 to 20 individuals. Fourteen primers of 300 arbitrary primers of 10 nucleotides showed reproducible polymorphism across the breeds. An amplified band of 0.9 kb in the primer MG-3 showed the specificity to Holstein breed. And MG-6 and MG-12 detected the Hereford and Hanwoo specific markers at the size of 2.0 kb and 1.0 kb, respectively. A 1.0 kb band of MG-12 was cloned and sequenced. A SCAR primer was designed based on the obtained sequences. It was possible to identify the Hanwoo from Holstein breed. Second experiment was carried out to observe the genotype frequencies of MC1R in 1,044 samples of imported beef and eight different cattle breeds including Hanwoo, Holstein, Angus, Brown-Swiss, Charolais, Limousin, Simmental and Hereford. The primers for the amplification of bovine MC1R gene were designed based on a bovine MC1R gene sequence (GenBank accession no.Y19103). A size of 350 bp was amplified by polymerase chain reaction(PCR), digested with two different restriction enzyme, BsrFI and MspA II, and electrophoresed in 2.5% Metaphore agarose gel for determination of genotypes. Genotype frequencies of Hanwoo were 0.10 in E+e and 0.90 in ee. Allele ED was shown in all of Holstein and Angus breeds tested which have black coat color phenotypes. We suggested that SCAR marker and the bovine MC1R gene could be used as a DNA marker for distinguishing beef between Hanwoo and Holstein.

• Proceedings of the Korean Society of Broadcast Engineers Conference
• /
• 2009.01a
• /
• pp.158-163
• /
• 2009

In recent years, there has been increased interest in characterizing and extracting 3D information from 2D images for human tracking and identification. In this paper, we propose a single view-based framework for robust estimation of height and position. In the proposed method, 2D features of target object is back-projected into the 3D scene space where its coordinate system is given by a rectangular marker. Then the position and the height are estimated in the 3D space. In addition, geometric error caused by inaccurate projective mapping is corrected by using geometric constraints provided by the marker. The accuracy and the robustness of our technique are verified on the experimental results of several real video sequences from outdoor environments.

• Proceedings of the Korea Multimedia Society Conference
• /
• 2001.11a
• /
• pp.596-601
• /
• 2001

본 논문에서는 두 대의 카메라로부터 입력받은 인간의 신체와 같은 관절체의 움직임을 Color Marker의 색상 특성을 이용하여 3차원 공간 정보를 인식하는 방법에 관해 기술한다. 이 방법은 인체에 물리적인 장치를 하지 않고 단순히 영상정보만을 이용하여 3차원 정보를 구하였다. 보다 정확한 인체의 특징점을 구하기 위해 환 논문에서는 신체의 관절각에 칼라 마커를 부착하고 칼라 마커틀을 세그먼트하여 관절각의 2차원 정보를 구한 다음 스테레오 기하(Stereo Geometry)를 이용하여 3차원 정보를 계산하고 가상 공간상에서 인간의 움직임을 추적하는 방법을 제안한다. 제안하는 방법은 제스쳐 인식과 3차원 Virtual Reality 인터페이스 시스템 구성 등에 사용될 수 있다.

• Journal of the Korea Society of Computer and Information
• /
• v.17 no.7
• /
• pp.155-166
• /
• 2012

This paper proposes a new method of estimating the hand pose through the Mean-Shift tracking algorithm using the fusion of color and depth information for marker-less augmented reality. On marker-less augmented reality, the most of previous studies detect the hand region using the skin color from simple experimental background. Because finger features should be detected on the hand, the hand pose that can be measured from cameras is restricted considerably. However, the proposed method can easily detect the hand pose from complex background through the new Mean-Shift tracking method using the fusion of the color and depth information from 3D sensor. The proposed method of estimating the hand pose uses the gravity point and two random points on the hand without largely constraints. The proposed Mean-Shift tracking method has about 50 pixels error less than general tracking method just using color value. The augmented reality experiment of the proposed method shows results of its performance being as good as marker based one on the complex background.

• Horticultural Science & Technology
• /
• v.31 no.1
• /
• pp.72-79
• /
• 2013

Inactivation of the gene coding for dihydroflavonol 4-reductase (DFR) is responsible for the color difference between red and yellow onions (Allium cepa L.). Two inactive DFR-A alleles, DFR-$A^{PS}$ and DFR-$A^{DEL}$, were identified in our previous study. A functional marker was developed on the basis of the premature stop codon that inactivated the DFR-$A^{PS}$ allele. A derived cleaved amplified polymorphic sequences (dCAPS) primer was designed to detect the single nucleotide polymorphism, an A/T transition, which produced the premature stop codon. Digested PCR products clearly distinguished the homozygous and heterozygous red $F_2$ individuals. Meanwhile, to develop a molecular marker for detection of the DFR-$A^{DEL}$ allele in which entire DFR-A gene was deleted, genome walking was performed and approximately 3 kb 5' and 3' flanking sequences of the DFR-$A^R$ coding region were obtained. PCR amplification using multiple primers binding to the extended flanking regions showed that more of the extended region of the DFR-A gene was deleted in the DFR-$A^{DEL}$ allele. A dominant simple PCR marker was developed to identify the DFR-$A^{DEL}$ allele using the dissimilar 3' flanking sequences of the DFR-A gene and homologous DFR-B pseudogene. Distribution of the DFR-$A^{PS}$ and DFR-$A^{DEL}$ alleles in yellow onion cultivars bred in Korea and Japan was surveyed using molecular makers developed in this study. Results showed predominant existence of the DFR-$A^{PS}$ allele in yellow onion cultivars.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.17 no.6
• /
• pp.1275-1282
• /
• 2022

In this study, real-time character navigation using AR lens developed by Nreal is developed. Real-time character navigation is not possible with general marker-based AR because NPC characters must guide while moving in an unspecified space. To replace this, a markerless AR system was developed using Digital Twin technology. Existing markerless AR is operated based on hardware such as GPS, gyroscope, and magnetic sensor, so location accuracy is low and processing time in the system is long, resulting in low reliability in real-time AR environment. In order to solve this problem, using the SLAM technique to construct a space into a 3D object and to construct a markerless AR based on point location, AR can be implemented without any hardware intervention in a real-time AR environment. This real-time AR environment configuration made it possible to implement a navigation system using characters in tourist attractions such as Suncheon Bay Garden and Suncheon Drama Filming Site.

• Korean Journal Plant Pathology
• /
• v.12 no.1
• /
• pp.41-46
• /
• 1996

본 실험은 이병 딸기의 조직에서 분리 동정된 시들음병균(Fusarium oxysporum f. sp. fragariae) 균주들의 유？거 변이를 random amplified polymorphic DNA(RAPD) marker들을 이용하여 조사하였다. 총 24개의 딸기 시들음병 균주들의 DNA를 주형으로 하여 16개의 random 10-mer primer들을 사용하여 증폭시킨 결과 총 231개의 marker들을 이용하여 유전적 변이를 조사해 본 결과 크게 RAPD I과 RAPD II의 2개 그룹으로 나눌 수 있었다. RAPD I그룹에 속하는 균주는 VCG A에 속하는 Y1, K1, K2, K3, K4, N2, N3, N4-1, N6-1, N6-2, N8, N9, N10, M1-2-1 균주, VCG B에 속하는 M4-1 균주 그리고 VCG C에 속하는 N1, Y2 균주들이었고, RAPD II그룹에는 VCG B에 속하는 M1-1, M2-2-1, M2-4-2, M3-2, M3-3-2 균주와 VCG D에 속하는 N1 1 균주가 속하였다. 이들 2그룹 간에는 31%의 유사성이 있었다.