• Wind and Structures
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• v.34 no.3
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• pp.291-301
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• 2022

Although mostly used in wind turbine market, single rotor wind turbines have problems with transportation and installation costs due to their large size. In order to solve such problems, multi-rotor wind turbine is being proposed; however, light weight design of multi-rotor wind turbine is required considering the installation at offshore or deep sea. This study proposes the systematic design process of the multi-rotor wind turbine focused on its supporting structure with simultaneous consideration of static and dynamic behaviors in an ideal situation. 2D and successive 3D topology optimization process based on the density method were applied to minimize the compliance of supporting structure. To realize the conceptual design obtained by topology optimization for manufacturing feasibility, the derived 3D structure was modified to have shell structures and optimized again through parametric design using the design of experiments and the response surface method for detail design of their thicknesses and radii. The resultant structure was determined to satisfy the stress and the buckling load constraint as well as to minimize the weight and the resultant supporting structure were verified numerically.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.694-699
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• 2003

Simultaneous measurement with PLIF(Planar Laser-Induced Fluorescence) and Stereo-PIV(Stereoscopic Particle Image Velocimetry) was performed to investigate the structural characteristics of flow field in Rushton Turbine Mixer. Instantaneous 3D velocity fields are measured by two 2K ${\times}$ 2K CCD cameras focused on an object plane with the angular displacement methods while the concentration fields are obtained through the measurement of the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. Finally, the spatial structures of turbulent mixing around Rushton turbine were identified by the calculation of cross-correlation fields between the velocity and concentration field.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.213-225
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• 2016

To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.

• Nuclear Engineering and Technology
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• v.30 no.2
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• pp.91-98
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• 1998

Time history analysis is usually performed to characterize the nonlinear seismic behavior of a spent fuel storage rack(SFSR). In the past, the seismic analyses of the SFSR were performed with two-dimensional planar models, which could not account for torsional response and simultaneous multi-directional seismic input In this study, three-dimensional seismic analysis methodology is developed for the single SFSR using the ANSYS code. The 3D- Model can be used to determine the nonlinear behavior of the rack, i.e., sliding, uplifting, and impact evaluation between the fuel assembly and rack, and rack and the pool wall, This paper also reviews the 3-D modeling of the SFSR and the adequacy of the ANSYS for the seismic analysis. AS a result of the adquacy study, the method of ANSYS transient analysis with acceleration time history is suitable for the seismic analysis of highly nonlinear structure such as an SFSR but it isn't appropriate to use displacement time history of seismic input.

• Journal of the Korean Society of Radiology
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• v.85 no.3
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• pp.596-606
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• 2024

Purpose To examine the technical considerations of endovascular treatment for aortoiliac occlusive disease (AIOD) based on a 10-year experience in Songklanagarind Hospital. Materials and Methods This retrospective cohort study included 210 patients who underwent endovascular treatment for symptomatic AIOD between January 2010 and December 2020. The patients' clinical and lesion characteristics, including technical considerations of the procedure, were collected, analyzed, and stratified using the Transatlantic Inter-Society Consensus (TASC). Results Most patients (80%) in this study had chronic limb-threatening ischemia lesions, with an occlusion rate of 37%. The technical success rate of TASC C & D was lower than that of TASC A & B, 84.4% vs. 99.2% p ≤ 0.001. A technical success rate of 93.3% (14/15) was found for the femoral and brachial approach, compared with a success rate of 89.0% (57/64) for the unibifemoral approach in TASC C & D, without a statistically significant difference (p = 0.076). However, the puncture site complications in this route were up to 17.6%, which is the highest rate compared with other techniques. These complications could be treated either conservatively or minimally invasively. Conclusion In cases of failed femoral access, simultaneous femoral and brachial approaches improved the technical success rate of endovascular recanalization of TASC C & D aortoiliac occlusions.

• Toxicological Research
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• v.3 no.2
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• pp.73-80
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• 1987

Primary cultures of adult rat hepatocytes were used to study the cytotoxicity of D-galactosamine. Hepatocytes were isolated by a collagenase perfusion technique and maintained as monolayers in serum-free medium on collagen-coated culture dishes. Treatment of galactosamine to the culture markedly inhibited the uptake of ${\alpha}$-aminoisobutyric acid (AIB) inducible with glucagon and dexamethasone. At0.1 mM of galactosamine, AIB uptake was inhibited significantly when treated for 12 hr. At higher doses (0.25, 0.5 and 1.0mM), a significant inhibition was noticed after 1 hr exposure. Generally the magnitude of the inhibition was related to the dose and treatment time of galactosamine. Treatment of galactosamine also produced a dose- and treatment time-related suppression of the tyrosine aminotransferase (TAT) induction caused by dexamethasone. Meanwhile, uptake of ouabain was not affected by the treatment of galactosamine. The viability of the hepatocytes was decreased only slightly by the treatment of galactosamine; more than 87% of the cells excluded tryphane blue when treated 1 mM galactosamine for 12 hr. Galactosamine induced depressions of AIB uptake and TAT activity were prevented by the simultaneous addition of uridine to the culture. D-Galactosamine, cytotoxicity, hepatocytes culture, ${\alpha}$-aminoisobutyric acid uptake, tyrosine aminotransferase.

• YAKHAK HOEJI
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• v.58 no.5
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• pp.314-321
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• 2014

Cibotii Rhizoma, the dried rhizome of Cibotium barometz J. Smith (C. barometz), has long been used to treat bone or nervous system disorders. In this regard, we isolated three main phenolic compounds, onitin-4-O-${\beta}$-D-glucopyranoside (1), irisdichototins E & F epimeric mixture (2), and protocatechuic acid (3) from C. barometz methanol extract. In addition, we screened their antioxidative activities by DPPH, ABTS radical, and superoxide scavenging assays. Among these three compounds, irisdichototins E & F and protocatechuic acid showed strong antioxidant activities. Also, the antioxidant activities of the C. barometz extracts were proportional to the contents of irisdichototins E & F and protocatechuic acid, thus these two phenolic compounds could be main active compounds of C. barometz. In addition, onitin-4-O-${\beta}$-D-glucopyranoside is considered as a marker compound of C. barometz because this compound is specifically contained in C. barometz which belongs to Pteridophyta order. A rapid analysis method for the simultaneous determination of phenolic compounds was also developed by UPLC (Ultra Performance Liquid Chromatography). Using the developed method, the two active compounds (irisdichototins E & F and protocatechuic acid) and a marker compound (onitin-4-O-${\beta}$-D-glucopyranoside) were successfully quantified in 14 commercial samples that were collected from different regions.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.919-921
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• 2013

영상시장의 개척과 디지털 기술의 발전과 더불어 차세대 3D 입체영상기술에 대한 관심과 수요가 증가하고 있다. 입체 정보는 크게 '단안 입체 정보(monoscopic depth cue)'와 '양안 입체 정보(stereoscopic depth cue)'로 분류 할 수 있다. 단안 입체 정보는 은폐, 상대적 크기, 상대적 밀도, 시야 안의 높이, 공기투시, 운동투시, 초점조절인 7가지로 경험에 의한 입체감을 지각하는 것을 말하며 양안 입체 정보는 두 눈으로 볼 때 처음으로 깊이를 지각 할 수 있는 것으로 크게 '동시시(simultaneous perception)', '융합(sensory fusion)', '입체시(stereoscopic vision)'의 세종류의 기능으로 분류한다. 3D 촬영은 이 양안시의 원리를 이용하여 두 대의 카메라의 좌우 영상을 합성하여 깊이감 있는 영상을 만들어 내게 된다. 본 논문에서는 3D 촬영방법은 촬영방식에 따라 크게 평행방식, 직교방식, 교차방식이 있는데 이중 중 원거리 촬영에 유리한 교차방식을 활용하여 사이드 바이 사이드 리그(Rig; 카메라를 수평으로 설치할 수 있도록 만들어진 장치)를 원거리 촬영에 맞게 축간거리를 기존의 리그 사이즈보다 2배 이상 긴 리그를 제작하여 보다 먼 거리에서의 상이한 좌우 영상획득이 가능하도록 설계하였다. 또한, 일정한 간격에 따라 피사체를 촬영하면서 거리에 따른 양 카메라의 가장 이상적인 IOD(Interocular Distance)와 CONV(Convergence)를 찾고, 교차방식촬영에 따른 특징적인 아티팩트인 키스톤 왜곡(Keystone distance)의 보정을 통한 원거리 입체영상을 효과적으로 획득하는데 본 연구방법을 제안하고자 한다.

• Journal of the Korean Society for Heat Treatment
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• v.14 no.1
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• pp.1-7
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• 2001

A method to simultaneously synthesize and consolidate the silicide $NiSi_2$ and the composite $NiSi_2$-20vol.%Nb from powders of Ni, Si, and Nb was investigated. Combustion synthesis was carried out under the combined effect of an electric field and mechanical pressure. The final density of the products increased nearly linearly with the applied pressure. Highly dense $NiSi_2$ and $NiSi_2$-20vol.%Nb with relative densities of up to 97% were produced under the simultaneous application of a 60MPa pressure and a 3000A current on the reactant powders. The respective Vickers microhardness values for these materials were 6.0 and 5.8 GPa. From indentation crack measurements, the fracture toughness values for $NiSi_2$ and $NiSi_2$-20vol.%Nb were calculated to be 3.3 and 4.7 $MPa{\cdot}m^{1/2}$, respectively.

• Bulletin of the Korean Chemical Society
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• v.35 no.7
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• pp.1956-1964
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• 2014

To provide the scientific corroboration of the traditional uses of Akebia quinata (Thunb.) Decne., a detailed analytical examination of A. quinata stems was carried out using a reversed-phase high performance liquid chromatography (RP-HPLC) method coupled to photodiode array detector (PDA) for the simultaneous determination of four phenolic substances; cuneataside D (1), 2-(3,4-dihydroxyphenyl)ethyl-O-${\beta}$-D-glucopyranoside (2), 3-caffeoylquinic acid (3) and calceolarioside B (4). Particular attention was focused on the main compound, 3-caffeoylquinic acid (3), which has a range of biological functions. In addition, 2-(3,4-dihydroxyphenyl)ethyl-O-${\beta}$-D-glucopyranoside (2) was considered as a discernible marker of A. quinata from its easy confuse plants. The contents of compounds 2 and 3 ranged from 0.72 to 2.68 mg/g and from 1.66 to 5.64 mg/g, respectively. The validation data indicated that this HPLC/PDA assay was used successfully to quantify the four phenolic compounds in A. quinata from different locations using relatively simple conditions and procedures. The pattern-recognition analysis data from 53 samples classified them into two groups, allowing discrimination between A. quinata and comparable herbs. The results suggest that the established HPLC/PDA method is suitable for quantitation and pattern-recognition analyses for a quality evaluation of this medicinal herb.