• Animal Systematics, Evolution and Diversity
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• 제38권1호
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• pp.26-33
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• 2022

Two Pseudopolydora polychaetes, P. bassarginensis and P. reticulata, originally described from Peter the Great Bay in Russia and Taiwan, respectively, were recorded firstly in Korea with DNA information. Two species are known to have distinct morphological characteristics that are separated from other Pseudopolydora species. They are characterized by reticulate pigmentations on the dorsal sides of the anterior chaetigers, a longitudinal black band-like pigmentation on the caruncle, and black paired spots on the ventral sides of the anterior chaetigers. These two species can be distinguished morphologically from each other by the length of the caruncle. Methyl green staining pattern of the species is a good method for delimiting Pseudopolydora species. The partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA (16S rDNA), and the nuclear 18S ribosomal DNA (18S rDNA) from Korean specimens of the two species were determined. The morphological descriptions and images of the two Pseudopolydora species are provided.

• 미생물학회지
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• 제26권4호
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• pp.312-317
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• 1988

The genes for ribosomal RNA in Rhizobium meliloti and Bradyrhizobium japonicum were analyzed by southern hybridization of BamHI, EcoRI, HindIII digested chromosomal DNA with purified 5' $^{32}P$-labeled 16S and 23S rRNA. The big differences in the hybridization pattern of both rhizobia were found. The comparative results were discussed in relation to the copy number and conservativity of restriction sites in the rRNA genes of both rhizobia.

• 생명과학회지
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• 제26권11호
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• pp.1341-1344
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• 2016

보길도에서 자라고 있는 황칠나무(Dendropanax morbifera)를 구입하여, 캘러스로 유도한 후, ribosomal DNA(nrDNA)의 internal transcribed spacer (ITS) region의 염기서열을 결정하였다 보길도의 황칠나무(Dendropanax morbifera)의 ITS region의 염기서열을 분석한 결과, 총 689염기를 결정하였다. 결정된 689염기 중에서 ITS1은 222 개염기, 5.8S rDNA는 160염기, ITS2는 233염기인 것으로 판명되었다. GenBank의 BLAST 프로그램(http://www.ncbi.nlm.nih.BLAST)을 사용하여 GenBank/EMBL/DDBJ에 등록되어 있는 Dendropanax 속 33의 염기서열을 수집한 후 multiple alignment를 수행한 결과, 유사도는 99.7%(D. chevalieri)에서 92.6%(Dendropanax arboreus)로 나타났으며, 일본황칠나무(D. trifidus)와는 유사도가 99.4%로 판명되었다.

• 식물분류학회지
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• 제53권1호
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• pp.32-37
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• 2023

Spiraea prunifolia f. simpliciflora Nakai is a perennial shrub widely used for horticultural and medicinal purposes. We simultaneously obtained the complete plastid genome (plastome) and nuclear ribosomal gene transcription units, 45S nuclear ribosomal DNA (nrDNA) and 5S nrDNA of S. prunifolia f. simpliciflora, using Illumina short-read data. The plastome is 155,984 bp in length with a canonical quadripartite structure consisting of 84,417 bp of a large single-copy region, 18,887 bp of a short single-copy region, and 26,340 bp of two inverted repeat regions. Overall, a total of 113 genes (79 protein-coding genes, 30 tRNAs, and four rRNAs) were annotated in the plastome. The 45S nrDNA transcription unit is 5,848 bp in length: 1,809 bp, 161 bp, and 3,397 bp for 18S, 5.8S, and 26S, respectively, and 261 bp and 220 bp for internal transcribed spacer (ITS) 1 and ITS 2 regions, respectively. The 5S nrDNA unit is 512 bp, including 121 bp of 5S rRNA and 391 bp of intergenic spacer regions. Phylogenetic analyses showed that the genus Spiraea was monophyletic and sister to the clade of Sibiraea angustata, Petrophytum caespitosum and Kelseya uniflora. Within the genus Spiraea, the sections Calospira and Spiraea were monophyletic, but the sect. Glomerati was nested within the sect. Chamaedryon. In the sect. Glomerati, S. prunifolia f. simpliciflora formed a subclade with S. media, and the subclade was sister to S. thunbergii and S. mongolica. The close relationship between S. prunifolia f. simpliciflora and S. media was also supported by the nrDNA phylogeny, indicating that the plastome and nrDNA sequences assembled in this study belong to the genus Spiraea. The newly reported complete plastome and nrDNA transcription unit sequences of S. prunifolia f. simpliciflora provide useful information for further phylogenetic and evolutionary studies of the genus Spiraea, as well as the family Rosaceae.

• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1644-1650
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• 2013

Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expression in seven tissues by real time PCR and protein expression in testis tissue by immunohistochemisty. Sequence analysis indicated that the obtained goat S6 was a 808 bp product, including a 3' untranslated region of 58 bp and an open reading frame of 750 bp which predicted a protein of 249 amino acids. The predicted amino acid sequence was highly homologous to cattle, human, mouse and rat S6. Expression analysis indicated S6 mRNA was expressed extensively in detected tissues and S6 protein was expressed in testis tissue.

• Animal cells and systems
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• 제3권1호
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• pp.9-15
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• 1999

Monophyly and intergeneric relationships of the tribe Helleboreae, sensu Tamura, and related genera were studied using a 1,100-bp segment at the 5'end of the 26S ribosomal RNA gene. Forty-one OTUs, including eight species of the Helleboreae, were either directly sequenced or obtained from previous publications. Data were analyzed using distance and discrete character methods to infer phylogenetic relationships among the included taxa. The inferred phylogeny did not support monophyly of either Helleboreae or Cimicifugeae whose members were intermixed in our inferred phylogeny. This result is congruent with our previous study, which recommended against finely subdividing, suprageneric higher taxa within the R-chromosome group (subfamily Ranuncluloideae, sensu lato) until more molecular data were accumulated. Our data convincingly suggest the presence of the following three monophyletic groups: the Cimicifuga group (the clade of Actaea, Cimicifuga, Souliea, Eranthis, Anemonopsis, and Beesia), the Trollius group (the clade of Trollius, Megaleranthis, Adonis), and a clade including Anemonopsis and Beesia. Our data also suggest that Trollius and Megaleranthis might be congeners and Eranthis a paraphyletic group.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1057-1064
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• 2013

p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that encodes the goat p70S6K gene 40S ribosomal S6 kinase 1 (S6K1) (GenBank accession GU144017) and its 3' noncoding sequence in Inner Mongolia Cashmere goats (Capra hircus). Goat S6K1 cDNA was 2,272 bp and include an open reading frame (ORF) of 1,578 bp, corresponding to a polypeptide of 525 amino acids, and a 694-residue 3' noncoding sequence with a polyadenylation signal at nucleotides 2,218 to 2,223. The relative abundance of S6K1 mRNA was measured by real-time PCR in 6 tissues, and p70S6K expression was examined by immunohistochemistry in heart and testis. The phosphorylation of p70S6K is regulated by mitogen-activated protein kinase (MAPK) signaling in fetal fibroblasts.

• 한국육종학회지
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• 제42권2호
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• pp.163-168
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• 2010

본 실험은 곡류 작물중에서 육종의 소재로써 많은 장점을 지닌 호밀을 Gimsa C-분염법과 FISH기법을 이용하여 구성이질 염색질과 5S와 18S-26S rRNA 유전자의 염색체상의 위치를 확인하고자 본 실험을 수행하였다. 그 결과를 요약하면 아래와 같다. 표지되었으며 2차협착으로 부수체가 존재하는 1번 염색체의 부수체의 말단과 5번 염색체의 중간에 표지되었고, 18S-26S rDNAs 유전자는 1개의 염색체에 표지되었으며 이 염색체는 2차협착으로 부수체가 존재하는 1번 염색체의 인 형성체 부위에 표지되었다. 1번 염색체에는 5S 와 18S-26S rDNAs 유전자가 표지되었고 5번 염섹체에는 5S rDNA 유전자만이 표지되었다.

• Mycobiology
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• 제40권1호
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• pp.42-46
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• 2012

This report describes the isolation and identification of a potent acetylcholinesterase (AChE) inhibitor-producing yeasts. Of 731 species of yeast strain, the S-3 strain was selected as a potent producer of AChE inhibitor. The selected S-3 strain was investigated for its microbiological characteristics. The S-3 strain was found to be short-oval yeast that did not form an ascospore. The strain formed a pseudomycelium and grew in yeast malt medium containing 50% glucose and 10% ethanol. Finally, the S-3 strain was identified by its physiological characteristics and 26S ribosomal DNA sequences as $Yarrowia$ $lipolytica$ S-3.

• 한국식품위생안전성학회지
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• 제35권1호
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• pp.45-50
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• 2020

본 연구는 원주시 내 대형 초밥 뷔페에서 제공되는 초밥, 회 등 26개 수산물 가공품을 대상으로 DNA 바코드를 분석하여 원재료의 종을 동정하였다. DNA 바코드 증폭을 위하여 미토콘드리아의 16S ribosomal RNA 및 cytochrome c oxidase subunit I 유전자 부위를 증폭하는 프라이머 세트를 이용하여 증폭된 PCR 산물의 염기서열을 분석하였다. 확보한 염기서열은 BLAST Search를 이용하여 미국국립보건원 GenBank에 등록되어있는 생물 종의 염기서열과 비교하여 염기서열 유사도와 매칭 점수를 고려하여 최종 종을 동정하였다. 모니터링 결과 분석 제품의 58%는 제품명과 사용된 원재료가 일치하였지만, 27%에서는 불일치가 관찰되었다. 초메기초밥에는 가이양(Pangasianodon hypophthalmus)이 꽃돔회에는 붉평치(Lampris guttatus)가 사용되었으며, 날치알군함 및 청어알무침에는 열빙어(Mallotus villosus) 알이 사용되었음을 확인하였다. 타코와사비군함 및 오징어간장소스에 사용된 원재료는 주꾸미(Amphioctopus fangsiao) 및 남방주꾸미(Amphioctopus membranaceus)로 각각 동정되었다.