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Effect of Antimicrobiotic of Opuntia ficus-indica on Surface Disinfection (제주도 손바닥선인장 추출물의 표면소독제로써의 항균효과)

  • Kwon, Young-ran;Kim, Da-Song;Shin, Hyun-Jae
    • Journal of Korean Biological Nursing Science
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    • v.23 no.1
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    • pp.1-10
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    • 2021
  • Purpose: To verify the anti-microbiotic activity and to develop eco-friendly surface disinfectants using the Opuntia ficus-indica extract. Methods: The stem and fruits of the Opuntia cultivated in Jeju Island were extracted with hot water, 70% EtOH and 100% MeOH, respectively. To examine the antimicrobial activity of the extracts, gram positive bacteria (Bacillus subtilis, Staphylococcus aureus), gram negative bacteria (Escherichia coli, Salmonella typhimurium), yeast (Candida albicans), mold (Aspergillus flavus) and antibiotic resistant bacteria (E. coli, S. aureus) were measured to form a clear zone. Results: The antibacterial activity of the fruit extract was higher than that of the stem extract, and the antibacterial activity of the 100% MeOH (FM) extract and the 70% EtOH (FE) extract of the palm cactus fruit was suitable for the surface disinfectant overall, and clear-zone formed even at relatively low concentrations (250 mg/mL). The FM and FE have antibacterial activity against various bacteria, therefore they can be used as disinfectants. Conclusion: The antimicrobial activity of Opuntia ficus-indica extract from Jeju Island by stem and fruit was determined at relatively low concentrations (250 mg/mL). The Opuntia ficus-indica extract will be useful for surface disinfectants dissolving the patients' bath, oral gargling, and powdered powder in water or ethanol in appropriate concentrations.

바이오매스 구성성분 중 리그닌의 전환에 관한 연구

  • Yun, Seong-Uk;Lee, Byeong-Hak
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.733-736
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    • 2000
  • Lignin is usable as fuels and heavy oil additives if depolymerized to monomer unit, because the chemical structures are similar to high octane materials found in gasoline. In this study, the solvent-phase thermal cracking(solvolysis) of lignin was performed at the various temperature and time in a laboratory tubular reactor. Conversion yield was measured for the properties of thermal cracking and liquefaction reaction of lignin. Highest conversion yield when acetone was used as thermal cracking solvent was 55.5% at $350^{\circ}C$, 50minutes and highest tar generation were $260{\sim}350mg/g\;{\cdot}\;lignin$ at $250^{\circ}C$, and highest conversion yield after tar removal was 76.88% at $300^{\circ}C$, 30minutes. Conversion yield, product compositions and amounts were determined by tar degradation yield.

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Protective Effects of Fucoidan against Acute Alcohol-induced Liver Injury in Rats (후코이단이 쥐의 급성 알콜성 간손상에 미치는 영향)

  • Kim, Mi-Ja;Jeon, Joseph;Lee, Sung Pyo;Lee, Jin-Sil
    • Korean Journal of Food Science and Technology
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    • v.46 no.2
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    • pp.219-223
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    • 2014
  • The purpose of this study was to investigate the protective effects of fucoidan on acute alcohol-induced liver injury in rats. Experimental animals were randomly divided into four groups: (1) a control group, (2) a group fed 25% ethanol, (3) a group fed 25% ethanol and 250 mg/kg BW of fucoidan (25% ethanol+FUCO250), and (4) a group fed 25% ethanol and 500 mg/kg BW of fucoidan (25% ethanol+FUCO500). Each group was fed orally two times per day for 15 days. Liver weights in the 25% ethanol group were increased compared to the control group, while liver weights in the 25% ethanol+FUCO500 group were significantly decreased compared to the 25% ethanol group (p<0.05). Plasma concentrations of triglyceride, cholesterol, and LDL-cholesterol were elevated in the 25% ethanol group; however, these levels in the 25% ethanol+FUCO250 group were significantly decreased compared to the 25% ethanol group (p<0.05). The glutamic-pyruvic transaminase activity of the 25% ethanol+FUCO500 group also was significantly lower than the 25% ethanol group (p<0.05). These results indicate that fucoidan might protect against acute alcohol-induced liver injury.

Development of MgH2-Ni Hydrogen Storage Alloy Requiring No Activation Process via Reactive Mechanical Grinding

  • Song, Myoung Youp;Kwak, Young Jun;Lee, Seong Ho;Park, Hye Ryoung
    • Korean Journal of Metals and Materials
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    • v.50 no.12
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    • pp.949-953
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    • 2012
  • $MgH_2$ was employed as a starting material instead of Mg in this work. A sample with a composition of 94 wt% $MgH_2-6$ wt% Ni (called $MgH_2-6Ni$) was prepared by reactive mechanical grinding. The hydriding and dehydriding properties were then examined. An $MgH_2-Ni$ hydrogen storage alloy that does not require an activation process was developed. The alloy was prepared in a planetary ball mill by grinding for 4 h at a ball disc revolution speed of 250 rpm under a hydrogen pressure of about 12 bar. The sample absorbed 3.74 wt% H for 5 min, 4.07 wt% H for 10 min, and 4.41 wt% H for 60 min at 573 K under 12 bar $H_2$, and desorbed 0.93 wt% H for 10 min, 1.99 wt% H for 30 min, and 3.16 wt% H for 60 min at 573 K under 1.0 bar $H_2$. $MgH_2-6Ni$ after reactive mechanical grinding contained ${\beta}-MgH_2$ (a room temperature form of $MgH_2$), Ni, ${\gamma}-MgH_2$ (a high pressure form of $MgH_2$), and a very small amount of MgO. Reactive mechanical grinding of Mg with Ni is considered to facilitate nucleation, and to reduce the particle size of Mg. $Mg_2Ni$ formed during reactive mechanical grinding also increases the hydriding and dehydriding rates of the sample.

Influences of Hydrocortisone, DHEA, Estradiol and Testosterone on the Polyamine Metabolism of Mouse Brain, Kidney, Liver and Intestine (Glucocorticoid 및 성(性) Steroid 홀몬에 의한 뇌(腦) 및 복부내(腹部內) 장기(臟器)의 Polyamine 대사(代謝)의 변동(變動)에 관한 연구(硏究))

  • Choi, Sang-Hyun;Chun, Boe-Gwun;Chun, Jong-Cheol;Chun, Yeon-Sook
    • The Korean Journal of Pharmacology
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    • v.27 no.1
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    • pp.81-88
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    • 1991
  • The bilateral castration of male mice was operated under light ether anesthesia, and the sham operated mice were considered as the uncastrated. The treatments of mice with the following steroids were started one hour after operation. Hydrocortisone 50 mg/kg (HC), dehydroepiandrosterone 250 mg/kg (DHEA), ${\beta}-estradiol$ 5 mg/kg (E2), and testosterone 20mg/kg (TS) were subcutaneously injected into male ICR mice at noon for four days. Animals were sacrificed in the next-morning (at 10-12 A.M.) after the last injection. The intestinal putrescine(PT) content was lower and the liver and intestinal spermine(SM) contents were higher in castrated mice(CM), comparing with those of uncastrated mice (UCM). The intestinal PT content of UCM was markedly increased HC. But all brain polyamines of CM were significantly decreased by it. And HC also increased the spermidine(SD) content of kidney and liver and the intestinal PT content in CM. E2 induced the marked increase of liver PT content with the moderate increase of renal SD in UCM. And E2 significantly increased the brain and liver PT contents and the all renal polyamine contents in CM. Both of DHEA and TS induced the increase of renal PT content in UCM, and they also induced the marked increases of all renal polyamines of CM. In addition, TS increased the brain SM of CM. These results suggest that the steroidal regulation mechanism of brain, kidney, liver, and intestine seems to be different from one another, and the renal activity of polyamine synthesis can be markedly enhanced by sex steroids.

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In Vitro Antioxidant Activity of Alnus firma Extracts (사방오리(Alnus firma) 추출물의 in vitro 항산화 활성)

  • Choi, Hye Jung;Joo, Woo Hong
    • Journal of Life Science
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    • v.29 no.2
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    • pp.231-238
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    • 2019
  • This study evaluated the antioxidant activity of the extract and fractions of Alnus firma. Alnus firma had the highest total phenolic content ($452.80{\pm}7.01{\mu}g$ gallic acid equivalents/mg) in a methanol (MeOH) fraction and the highest total flavonoid content ($112.29{\pm}11.14{\mu}g$ rutin equivalents/mg) and antioxidant capacity ($936.23{\pm}0.07{\mu}g$ ${\alpha}$-tocopherol equivalents/mg) in an ethylacetate (EA) fraction. The antioxidant activities of various solvent extract fractions of Alnus firma were evaluated using various antioxidant assays, including ${\beta}$-carotene-linoleate assay, reducing power assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, metal chelating activity assay, superoxide anion radical scavenging assay, and lipid peroxidation inhibition assay using the ferric thiocyanate method. These activities were compared with those of ascorbic acid, butylated hydroxyanisole (BHA), gallic acid (GA), butylated hydroxytoluene (BHT), and ${\alpha}$-tocopherol. First, at a $250{\mu}g/ml$ concentration, the EA and MeOH fractions of A. firma showed 92.43% and 89.20% DPPH radical scavenging activity, respectively. Second, $50{\mu}g/ml$ of the EA fraction exhibited 72.49% superoxide anion radical scavenging activity, a little greater than the same dose of GA (60.88%). Finally, 0.5 and 1 mg/ml of the EA fraction showed 73.45% and 73.29% inhibition of peroxidation in the ${\beta}$-carotene-linoleic acid system, respectively. The decreasing order of reducing power was EA fraction > n-butanol (BuOH) fraction > dichloromethane (DCM) fraction > n-hexane (HX) fraction. The results obtained in the present study indicated that Alnus firma can be used as an easily accessible potential source of natural antioxidants.

Rooting Characteristics of Sageretia thea by Cutting Time, Rooting Medium and Plant Growth Regulators (삽목시기, 삽목용토, 생장조절제에 따른 상동나무 삽목발근 특성)

  • Son, Yonghwan;Park, Sunghyuk;Son, Hojun;Kim, Ji-Ah;Cho, Hyejung;Lee, Sun-Young;Kim, Hyun-Jun
    • Korean Journal of Plant Resources
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    • v.35 no.5
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    • pp.667-674
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    • 2022
  • This study was conducted to determine the three main factors [rooting time, plant growth regulators (PGRs), rooting medium] of cuttage for mass production of Sageretia thea. The experiment was observed on cuttage tray after 120 days and classified by rooting times, plant growth regulators, rooting medium. Materials were collected from natural habitats of S. thea from the Korean peninsula at March, July and September during 2019. As a result of the experiment, rooting times were showed statistically significant differences. March group's rooting rate and root length were 15 and 200% better than others. In PGRs experiment by concentration, There was no significant correlation between the control and experiment in terms of rooting rate. However, In the case of root length, IBA 500 mg/L, IBA 250 mg/L and IAA 1,000 mg/L groups were lengthened up to 170% compared to the control group, confirming statistically significant differences. As a result, we recommend treating cuttings with IBA 500 mg/L in March with Hardwood cutting.

Effect of Additives on Densification and Grain Growth of Magnesia (마그네시아의 치밀화 및 입자성장에 미치는 첨가물의 영향)

  • Lee, Hae-Weon;Kim, Whan
    • Journal of the Korean Ceramic Society
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    • v.19 no.2
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    • pp.127-132
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    • 1982
  • This experiment has been carried out for the purpose of investigating the effect of additives on densification and grain growth in magnesium oxide by a two-step process; hot pressing and heat treating. MgO powder has been obtained by calcining extra reagent grade MgCO3 at 90$0^{\circ}C$ for 30 minutes, and additives have been added to $MgCO_3$ in the form of soluble salts-Al$(NO_3)_3$$. $9H_2O$ and $Cr(NO_3)_3$.9H_2O$. The hot pressing has been carried out with changes of soaking time at 125$0^{\circ}C$ under the pressure of 250kg/$\textrm{cm}^2$, and the heat treating also at same temperature. The initial particle size of MgO measured by particle size analyzer was 0.86 microns. Densification rate obeyed the equation D=K lnt + C, and grain growth rate obeyed the equation G-G0=kt1/2. It was vaporization of some $Cr_2O_3$ and formation of solid solution that had an influence on desification of MgO containing $Cr_2O_3$. Activation energy for grain growth of pure MgO was 62.4 kcal/mole, therefore grain growth was supposed to be diffusioncontrolled process. But after heat treatmeat, excess additives were expected to slow down the grain growth by the formation of second phase or the solute atoms at grainboundary.

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Highly Sensitive and Selective Ethanol Sensors Using Magnesium doped Indium Oxide Hollow Spheres

  • Jo, Young-Moo;Lee, Chul-Soon;Wang, Rui;Park, Joon-Shik;Lee, Jong-Heun
    • Journal of the Korean Ceramic Society
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    • v.54 no.4
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    • pp.303-307
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    • 2017
  • Pure $In_2O_3$, 0.5 and 1.0 wt% Mg doped $In_2O_3$ hollow spheres were synthesized by ultrasonic spray pyrolysis of a solution containing In-, Mg-nitrate and sucrose and their gas sensing characteristics to 5 ppm $C_2H_5OH$, p-xylene, toluene, and HCHO were measured at 250, 300 and $350^{\circ}C$. Although the addition of Mg decreases the specific surface area and the volume of meso-pores, the gas response (resistance ratio) of the 0.5 wt% Mg doped $In_2O_3$ hollow spheres to 5 ppm $C_2H_5OH$ at $350^{\circ}C$ (69.4) was significantly higher than that of the pure $In_2O_3$ hollow spheres (24.4). In addition, the Mg doped $In_2O_3$ hollow spheres showed the highest selectivity to $C_2H_5OH$. This was attributed to the dehydrogenation of $C_2H_5OH$ assisted by basic MgO into reactive $CH_3CHO$ and $H_2$.

A Study on the Contents of Tryptophan and Available Lysine in Korean Foods (수종한국상용식품중(數種韓國常用食品中)의 Tryptophan 및 Available lysine 함량(含量)에 대(對)하여)

  • Kim, Soong-Won;Lee, Sung-Dong
    • Journal of Nutrition and Health
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    • v.12 no.2
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    • pp.65-74
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    • 1979
  • In order to observe the contents of some essential amino acids in Korean foods, total and free tryptophan, and available lysine in fifty kinds of Korean foods were analyzed by the sfectrophotometry. The results obtained are summarized as follows : 1) The tryptophan contents per 100g of soybean, wet green laver and skim milk were over 250 mg, and the contents Per g nitrogen in ginger, dried persimmon and chestnut were over 150 mg. 2) The free tryptophan contents per 100 g of soybean, small red bean, dried persimmon, ginseng stem were over 100 mg, ana the contents per g nitrogen in dried persimmon was over 150 mg. 3) The available lysine contents Per 100g of soybean, wet green laver and skim milk, fish(auchovy), dried yeast, casein and silkworm pupa were over 1000 mg, and the contents per g nitrogen in potato, perilla(wild sesame), red pepper, sausage and skim milk were over 300mg. 4) The contents of tryptophan and available lysine in soybean, green laver and skim milk were higher than in other samples. 5) In general, the contents of tryptophan and available lysine were abundant in seaweeds.

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