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Antimicrobial Activities of Oak Smoke Flavoring (참나무 목초액의 항균효과)

  • Seo, Kwon-Il;Ha, Ki-Jeong;Bae, Young-Il;Jang, Jin-Kyu;Shim, Ki-Hwan
    • Food Science and Preservation
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    • v.7 no.3
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    • pp.337-341
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    • 2000
  • Antimicrobial activities of oak smoke flavoring against saprogenous and food poisoning microbes were studied. When tested on a paper disc, antimicrobial activities were observed in all microbial species at a dose of as low as 20 ${\mu}{\ell}$, and microbial growth was completely suppressed with 70 ${\mu}{\ell}/5$ $m{\ell}$ during 3 days of incubation at $30^{\circ}C$ with 8 times diluted oak smoke flavoring. When 50 $m{\ell}s$ of distilled water and distilled water containing 500 ${\mu}{\ell}$ of oak smoke flavoring, which were added with small amount of dilute E. coli, were incubated for 10 days at $20^{\circ}C$, $3{\times}10^3$ CFU/$m{\ell}$ of E. coli and $1.9{\times}10^4$ CFU/$m{\ell}$ of total bacteria was counted in distilled water containing oak smoke flavoring.

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Effects of Seeding Times on the Growth Characteristics and Seed Productivity of Orchardgrass (Dactylis glomerata L.) (파종시기가 오차드그라스의 생육특성 및 종자생산에 미치는 영향)

  • 최기준;정의수;임용우;임영철;김기용;성병렬;김맹중;박근제
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.23 no.2
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    • pp.101-106
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    • 2003
  • This experiment was carried out to investigate the growth characteristics and seed productivities of orchardgrass "Hwabsung 2 ho" according to seeding times in the NLRI from 2001 to 2002. The experimental design was a randomized block arrangement with three replications. Seed was sown on 10th, 20th, and 30th of Aug. and 10th, 20th, and 30th of Sept. in 2000 and 2001, respectively. Establishment of seedling was worse in seeding of Aug. 10th because of rainfall. Number of panicles per $1\textrm{m}^2$ was numerous in seeding of Aug. 20th and was fewer according as seeding time was later. But panicle did not nearly appear in seeding after Sept. 20th. Seeding of Aug. 20th produced seed yield of 759kg/ha and have most seed yield components. But according as seeding times were later than Aug. 20th, the seed yield was linearly decreased with seed yield components worse.nts worse.

Electrochemistry of bis(1,10-phenanthroline)copper(II)-sodium dodecyl sulfate solution in the presence of MgCl2 (염화마그네슘 존재 하의 비스(1,10-페난트롤린) 구리(II)-도데실황산나트륨 용액의 전기화학)

  • Ko, Young Chun
    • Analytical Science and Technology
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    • v.20 no.6
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    • pp.483-487
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    • 2007
  • Electrochemistry of 1.0 mM bis(1,10-phenanthroline)copper(II) $(Cu(ph){_2}^{2+})$ in 100 mM NaCl solution including 27 mM $MgCl_2$ with and without sodium dodecyl sulfate (SDS) is studied. In the presence of SDS, $E_{pa}$ and $E_{1/2}$ of $Cu(ph){_2}^{2+}$ by adding $Mg^{2+}$ shifts to a positive direction compared to the SDS free. The intersection of two lines on ${\Delta}E_p$ vs -log[SDS] plot is determined as a critical micelle concentration (CMC). When $Mg^{2+}$ is added, it seems that the double layer became more compact. And the formation of micelles is retarded.

A Rapid Screening for Aluminum-tolerant and -sensitive in Barley (Hordeum vulgare L.) and Plasma Membrane H+-ATPase Expression (알루미늄 내성과 민감성 보리의 빠른 screening과 원형질막 H+-ATPase의 발현)

  • Kim, Hyun-Sung;Oh, Jung-Min;Ahn, Sung-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.72-79
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    • 2011
  • Here we report a simple screening system using hematoxylin staining (HS) of the root apex. It allowed rapid classification into different aluminum (Al) tolerance from 65 cultivars within one week. Using this system, we selected the most Al-tolerant (Jayae-2) and-sensitive (Pum-2) barley (Hordeum vulgare L.) The results show that the different responses in Al-induced growth inhibition, Al accumulation and expression of plasma membrane (PM) $H^+$-ATPase in root apices of selected two cultivars. It showed strongly Al-induced growth inhibition in a dosedependant manner only in Pum-2 but not in Jayae-2. Aluminum accumulation in root apices (10 mm) was significantly higher in Pum-2 only. The $H^+$-ATPase expression of PM vesicles by western blotting was decreased in Pum-2 but not in Jayae-2 treated with $20{\mu}M$ Al for 24 h. These finding indicate to screen from our system is rapid and reliable and to sustain the expression of PM $H^+$-ATPase at translational level is an important role in root growth as affected by Al.

The Remediation Characteristic of Soil Contaminated with Heavy Metal and Total Petroleum Hydrocarbon (TPH) by Enhanced Electrokinetic with Fenton Oxidation and Soil Flushing Method (펜톤 산화와 토양 세정이 보강된 동전기에 의한 중금속 및 총 석유 탄화수소(TPH)로 오염된 토양의 정화 특성)

  • Seo, Seok-Ju;Na, So-Jeong;Kim, Jung-Hwan;Park, Joo-Yang
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.34 no.3
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    • pp.885-893
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    • 2014
  • This research reports the enhanced Electrokinetic (EK) with $H_2O_2$ and sodium dodecyl surfate (SDS), which are commonly used in Fenton oxidation and soil flushing method, in order to remediate soil contaminated with heavy metals and Total Petroleum Hydrocarbons (TPH) simultaneously. In addition, influences of property of soil and concentration of chemical solution were investigated through experiments of different types of soils and varying concentration of chemical reagents. The results indicated, in the experiments using artificially contaminated soil, the highest removal efficiency of heavy metals using 10% $H_2O_2$ and 20mM SDS as electrolytes. However, in the experiments using Yong-San soils (study area), remediation efficiency of heavy metals was decreased because high acid buffering capacity. Through experiment of 20% $H_2O_2$ and 40mM SDS, increased electric current influences the remediation of heavy metals due to decrease in the soil pH. In the experiments of Yong-San soils, the remediation efficiency of TPH was decreased compared with artificially spiked soils because high acid buffering capacity and organic carbon contents. Furthermore, the scavenger effect of SDS influenced TPH oxidation efficiency under the conditions of injected 40mM SDS in the soils. Therefore, the property of soil and concentration of chemical reagents cause the electroosmotic flow, soil pH, remediation efficiency of heavy metals and TPH.

Fast Factorization Methods based on Elliptic Curves over Finite Fields (유한체위에서의 타원곡선을 이용한 고속 소인수분해법에 관한 연구)

  • Kim, Yong-Tae
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.10
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    • pp.1093-1100
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    • 2015
  • Since the security of RSA cryptosystem depends on the difficulty of factoring integers, it is the most important problem to factor large integers in RSA cryptosystem. The Lenstra elliptic curve factorization method(ECM) is considered a special purpose factoring algorithm as it is still the best algorithm for divisors not greatly exceeding 20 to 25 digits(64 to 83 bits or so). ECM, however, wastes most time to calculate $M{\cdot}P$ mod N and so Montgomery and Koyama both give fast methods for implementing $M{\cdot}P$ mod N. We, in this paper, further analyze Montgomery and Koyama's methods and propose an efficient algorithm which choose the optimal parameters and reduces the number of multiplications of Montgomery and Koyama's methods. Consequently, the run time of our algorithm is reduced by 20% or so than that of Montgomery and Koyama's methods.

Two Cases of Corneal Edema Due to Vaporized Amine (기화된 아민에 의한 각막부종 2예)

  • Hwang, Yousook;Cho, Yang Kyung
    • Journal of The Korean Ophthalmological Society
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    • v.59 no.11
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    • pp.1077-1081
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    • 2018
  • Purpose: We report two cases of corneal edema in patients who presented with bilateral blurry vision due to vaporized amines while working in a polyurethane processing plant. Case summary: A 28-year-old male presented with bilateral blurred vision. His work involved solidifying polyurethane liquid and he often found himself exposed to polyurethane heat and gas. Purpose: We report two cases of corneal edema in patients who presented with bilateral blurry vision due to vaporized amines while working in a polyurethane processing plant. Case summary: A 28-year-old male presented with bilateral blurred vision. His work involved solidifying polyurethane liquid and he often found himself exposed to polyurethane heat and gas. On examination, the patient's uncorrected visual acuity (UCVA) was 20/40 (right) and 30/50 (left). A slit lamp examination revealed subepithelial microbullae in both eyes. The central corneal thickness (CCT) was also increased in both eyes, measuring $698{\mu}m$ (right) and $672{\mu}m$ (left). After prescribing 0.5% moxifloxacin and, 1% fluorometholone eye drops for 3 days in both eyes, the UCVA recovered to 20/40 (right) and 20/20 (left). The CCT decreased to $644{\mu}m$ (right) and $651{\mu}m$ (left), and the microbullae improved significantly in the left eye. The second patient was a 34-year-old female who presented with bilateral decreased visual acuity while at work. She worked in a factory that produced car seat filling. Her UCVA was 20/25 (right) and 20/20 (left). The CCT by specular microscopy was $537{\mu}m$ (right) and $541{\mu}m$ (left). On slit lamp examination, both eyes demonstrated bilateral central subepithelial edema. The patient did not attend any follow-up outpatient appointments after the initial presentation. Conclusions: Exposure to vaporized amines such as polyurethane may causereversible corneal toxicityeven without direct contact. Further consideration should be given to ocular safety and protection from amine compounds in the industrial field.On examination, the patient's uncorrected visual acuity (UCVA) was 20/40 (right) and 30/50 (left). A slit lamp examination revealed subepithelial microbullae in both eyes. The central corneal thickness (CCT) was also increased in both eyes, measuring $698{\mu}m$ (right) and $672{\mu}m$ (left). After prescribing 0.5% moxifloxacin and, 1% fluorometholone eye drops for 3 days in both eyes, the UCVA recovered to 20/40 (right) and 20/20 (left). The CCT decreased to $644{\mu}m$ (right) and $651{\mu}m$ (left), and the microbullae improved significantly in the left eye. The second patient was a 34-year-old female who presented with bilateral decreased visual acuity while at work. She worked in a factory that produced car seat filling. Her UCVA was 20/25 (right) and 20/20 (left). The CCT by specular microscopy was $537{\mu}m$ (right) and $541{\mu}m$ (left). On slit lamp examination, both eyes demonstrated bilateral central subepithelial edema. The patient did not attend any follow-up outpatient appointments after the initial presentation. Conclusions: Exposure to vaporized amines such as polyurethane may causereversible corneal toxicityeven without direct contact. Further consideration should be given to ocular safety and protection from amine compounds in the industrial field.

Two Cases of Long-Term Changes in the Retinal Nerve Fiber Layer Thickness after Intravitreal Bevacizumab for Diabetic Papillopathy (당뇨병유두병증에서 유리체강내 베바시주맙 주입술 후 망막시경섬유층 두께의 장기간 변화 2예)

  • Kim, Jong Jin;Im, Jong Chan;Shin, Jae Pil;Kim, In Taek;Park, Dong Ho
    • Journal of The Korean Ophthalmological Society
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    • v.54 no.9
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    • pp.1445-1451
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    • 2013
  • Purpose: To report long-term changes in the average retinal nerve fiber layer (RNFL) thickness in 2 patients who had intravitreal bevacizumab (IVB) injection for diabetic papillopathy. Case summary: A 36-year-old patient with diabetes complained of decreased visual acuity (20/200) in the right eye. The fundus examination showed optic disc swelling in both eyes. The average RNFL thickness based on optical coherence tomography (OCT) increased to $278{\mu}m$ and Goldmann perimetry showed nasal visual field defect in the right eye. The IVB was injected into the right eye. Three weeks after the IVB injection, RNFL thickness decreased to $135{\mu}m$ and visual acuity improved to 20/25 in the right eye. However, RNFL thickness increased from 126 to $207{\mu}m$ and visual acuity decreased to 20/32 in the left eye. Thus, IVB was injected into the left eye. In week 3, RNFL thickness decreased to $147{\mu}m$ and visual acuity improved to 20/20 in the left eye. At 12 months after IVB injection, RNFL thickness was $87{\mu}m$ in the right eye and $109{\mu}m$ in the left eye. A 57-year-old patient with diabetes complained of decreased visual acuity (20/200) and showed optic disc swelling in the right eye. The average RNFL thickness increased to $252{\mu}m$ and Goldmann perimetry showed an enlarged blind spot in the right eye. IVB was injected into the right eye. After 3 weeks, RNFL thickness decreased to $136{\mu}m$ and visual acuity improved to 20/70 in the right eye. Six months after IVB injection, RNFL thickness was $83{\mu}m$ in the right eye. Conclusions: Visual acuity progressively improved within 3 weeks and RNFL thickness measured by spectral domain OCT showed progressive thickness reduction in 2 cases of diabetic papillopathy patients who had IVB injections.

Discharge Characteristics of the Indicator Microorganisms of Combined Sewer Overflows (합류식 하수관거 월류수의 지표미생물 배출 특성)

  • Kim, Geonha
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.4
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    • pp.627-635
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    • 2006
  • Combined sewer overflow (CSOs) is a primary diffuse source degrading water quality of urban streams. In this study, CSOs caused by 5 different rainfall events at an urban watershed located in Daejeon city were monitored for the indicator microorganism concentrations. Event mean concentration (EMC) of the indicator microorganisms were: total coliform = $2.46{\times}10^6CFU/100mL$; fecal coliform = $1.01{\times}10^6CFU/100mL$; E.coli = $5.20{\times}10^5CFU/100mL$; and Fecal Streptococci = $6.08{\times}10^5CFU/100mL$. In addition, coliform concentrations were well correlated with suspended solid concentrations and the first flush effects were identified. Settling tests were carried out to estimate removal rate of indicator organisms by sedimentation from CSOs. As microorganisms are discharged in association with suspended solid, ten minutes of settlement can lower 44% of indicator microorganism leading.

Effect of Alpha Lipoic Acid as an Antioxidant Supplement during In Vitro Maturation Medium on Bovine Embryonic Development

  • Hassan, Bahia M.S.;Fang, Xun;Roy, Pantu Kumar;Shin, Sang Tae;Cho, Jong Ki
    • Journal of Embryo Transfer
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    • v.32 no.3
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    • pp.123-130
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    • 2017
  • This study was conducted to investigate the effects of alpha-lipoic acid (aLA) as an antioxidant that decrease the reactive oxygen species (ROS) in bovine embryonic development. Slaughterhouse derived bovine immature oocytes were collected and 4 different concentrations (0, 5, 10 and 20 mM) of aLA was supplemented in bovine in vitro maturation (IVM) medium. After 20 hrs of IVM, maturation rates, levels of ROS and glutathione (GSH), and further embryonic development after parthenogenetic activation (PA) and in vitro fertilization (IVF) was investigated according to aLA concentrations. Maturation rate was significantly higher in 10 mM group than other groups (80.5% vs. 62.9, 73.9, 64.2%; P<0.05). In the levels of ROS and GSH in matured oocytes as an indicator of oocyte quality, significantly better results were shown in 5 and 10 mM groups compared with other 2 groups. After IVM, significantly higher rates of blastocyst formation were shown in 10 mM groups in both of PA (27.9% vs. 18.8, 22.3, 14.2%; P<0.05) and IVF (32.6% vs. 23.9, 27.3, 16.2%; P<0.05) embryos. In addition, significantly more cell total cell number and higher inner cell mass ratio in 10 mM PA and IVP blastocysts showed developmental competence in 10 uM groups. Therefore, based on the entire data from this study, using $10{\mu}M$ of aLA confirmed to be the optimal concentration for bovine oocyte maturation and embryonic development.