• Korean Journal of Poultry Science
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• v.41 no.3
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• pp.205-215
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• 2014

The aim of this study was to determine the effects of stocking density and strain on the performance and physiological adaptive responses including the plasma corticosterone content and the level of mRNA expression of pro-inflammatory cytokines and antioxidant enzymes in broiler chicks. A total of 300 birds of two strains (150 Ross strain vs. 150 Cobb strain) aged 3-d old were allotted into two stocking densities (standard stocking density,$0.046m^2/bird$ vs. high stocking density, $0.023m^2/bird$) in battery cages by $2{\times}2$ factorial designs with ten replicates until 35 d of age. There was no significant strain effect on body weight, feed intakes and feed to gain ratio and the relative organ weights. However body weight, feed intakes and relative organ weight were found to be significantly (P<0.05) affected by the effect of stocking density. Plasma corticosterone level was not affected by both stocking density and strain effects. Hepatic mRNA expression of pro-inflammatory cytokines including interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6, IL-18 and interferon-gamma (IFN-${\gamma}$) was not significantly changed by the effects of strain and stocking density. However, the mRNA expression of glutathione peroxidase (GPX) was affected by strain, showing that Ross strain decreased (P<0.05) the GPX expression. With respect to the effect of stocking density, there was a significant (P<0.05) increase in superoxide dismutase (SOD) and catalase (CAT) and GPX mRNA expression in the liver from high stocking density group. Splenic pro-inflammatory cytokine expression was not also affected by stocking density and strain, except that IL-18 mRNA significantly (P<0.05) decreased in Cobb strain under high stocking density. The mRNA expression of SOD and CAT was significantly (P<0.05) affected by the effects of stocking density and strain. In conclusion, growth performance was not affected by strain but stocking density. Although mRNA expression of major pro-inflammatory cytokines was not changed by stocking density and strain, antioxidant enzyme was significantly affected by stocking density, strain or even organ in birds under summer conditions. More detailed studies still needed to be explored to elucidate the effects of environmental conditions and genetic background on physiological responses in birds.

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.3
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• pp.69-80
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• 2012

The purpose of this paper was to study the issues related to converting the graveyards within forests into spaces intended for tree burials by means of planting, given the situation that the graveyards have encroached on land and damaged the environment. For the reason, a field survey was performed to determine the width, length, and distance to the nearest tree of 205 graveyards in the capital area. Through this, it was determined that the domestic lands damaged by graveyards amounted to $862km^2$, including the areas that were deforested to manage the graves. This only confirms that land encroachment by graveyards is a serious issue. The methods for making tree burial sites were examined from the perspective of how to meet public demands given the graveyard's spatial distinctiveness. As a result, this study suggested different methods to establish tree burial sites according to the degree of transformation and the term of its formation. This study also classified the graveyards into three types, and identified the planting methods that harmonized the safe growth of trees and the scenic beauty of memorial places based on the standard. This is in order to plant trees that are shade-tolerant and suitable to the forest line, along with which other tree line was and also, to plant aesthetic trees around the empty space. Through applying the developed methods, this study established and monitored two exemplary sites in Yongin and Boryeng. Aesthetic trees were planted in Yongin site which was located in an open area, aod the shade-tolerant trees were planted in Boryeong, which was located in a forest area. As a result, the image of a garden appeared at Yongin site and the image of a tree colony harmonized with the near forest emerged at Boryeong site. Therefore, it is confirmed that the method of planting according to the distribution status of neighboring trees was effective. As a result of monitoring, mulching wood chips were suitable for sites that were small or easy to approach. This is because the weeds were controlled in Yongin site by mulching. Furthermore, by monitoring the growth of 11 species of vegetation, this study confirmed that low and cover-type vegetations were suitable for tree burial sites. In Boryeong site, the wild cherry trees, which were planted as adult trees, all died, and the tilling of snake's beard, which were planted as cover vegetation, was slow. Therefore, this study found that seedlings were more suitable to plant in forest graveyards than adult trees, which were large and difficult to approach, and it was effective to use the remaining lawn and form a low vegetation after the crown of trees had expanded to such places.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.3
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• pp.205-212
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• 2021

The skin fibroblasts of different origins showed different expression levels of MMP-1 in response to TNF-α treatment or UV irradiation. We hypothesized that this is caused by polymorphism in the MMP-1 promoter region. To elucidate it, first of all, we analyzed and classified the genotype of the -1607 site of the MMP-1 promoter in 23 commercially available primary fibroblasts, and then we examined the expression of MMP-1 by TNF-α or UVB stimulation for each classified genotype. As a result of the analysis, fibroblasts with 6 1G/1G genotypes, 10 1G/2G genotypes, and 7 2G/2G genotypes were identified. Hs68 and Detroit 551 cell lines were confirmed to have 1G/2G genotypes. In the 1G/1G genotype, MMP-1 was expressed twice as high as that of the control group by TNF-α treatment, and was hardly expressed by UV light. In the case of the 1G/2G genotype, MMP-1 was expressed 2.45 fold higher by TNF-α treatment, and 1.4 fold by UV light than the control. In the case of the 2G/2G genotype, MMP-1 was expressed 1.35 fold by TNF-α treatment, and was highly expressed by 2.5 fold by ultraviolet rays compared to control. It can be estimated that MMP-1 expression is better induced in the 1G genotype by TNF-α and in the 2G genotype by UV light. In addition, it can be presumed that MMP-1 expression is increased by creating a site where the Ets transcription factor can bind by another G inserted at the -1607 position. These studies have not been conducted at all in fibroblasts in relation to skin aging, so it is an area that needs to be further studied in the future. In conclusion, since the skin is an organ that is affected by both intrinsic aging and photoaging at the same time, when analyzing the expression of MMP-1 as a target for improving skin aging, it is necessary to select cells with a genotype suitable for the experimental conditions of the study.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.14 no.4
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• pp.205-212
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• 2009

To suggest possible to bioremediation by benthic microalgae Nitzschia sp. isolated from the Jinhae Bay, the studies investigated the effects o flight quality and quantity on the growth of Nitzschia sp. and its growth kinetics for phosphate investigated. The Nitzschia sp. was cultured under blue (450 nm), yellow (590 nm) and red wavelength (650 nm) using light emitting diode (LED) and mixed wavelengths using a fluorescent lamp. The maximum specific growth rate showed the Nitzschia sp. under blue wavelength, although photoinhibition was observed above $100\;{\mu}mol\;m^{-2}\;s^{-1}$. Mixed wavelengths were also observed by decreasing the maximum cell density from high irradiances (>$100\;{\mu}mol$ photons $m^{-2}\;s^{-1}$). The compensation photon flux density ($I_0$) calculated from the mixed wavelengths equated to a depth of 4-10 m in Jinhae Bay, and was lower in the summer season than the depth due to suspended matter (ca. 4 m). Thus, the suitable depth for maximum growth of Nitzschia sp. might be extremely limited. In the growth kinetics for phosphate, half-saturation constant ($K_s$) was similar among different wavelengths, although the maximum growth rate was varied among different wavelengths. Because the $K_s$ was high than that of the phytoplankton, Nitzschia sp. might have adapted to the high nutrient concentrations, and have effective nutrient storage in the cell quota. Thus, Nitzschia sp. may be a useful species for bioremediation of the benthic layer in polluted inner bays by means of irradiated specific wavelength as blue.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.58-59
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• 2003

연안정착성 어류인 쏨뱅이는 대부분의 볼락류와 더불어 자어를 출산하는 종이다. 그러므로 종묘생산을 원활히 수행하기 위해서는 건강한 자어를 안정적으로 확보하는 것이 무엇보다 중요한 요소이다. 이번 실험은 쏨뱅이 어미를 실내사육하고 자어 출산을 유도하여, 우량의 자어를 다량 확보할 수 있는 기초를 마련하고자 출산시기, 출산횟수, 자어마리수, 자어크기 등 쏨뱅이의 출산생태에 대하여 1997년부터 1999년까지 3년간 실내 자연출산에 관한 연구를 실시하였다. 1. 쏨뱅이 어미의 출산횟수. 쏨뱅이는 한 마리의 어미가 수회에 걸쳐 자어를 출산하는 것으로 알려져 있다. 1997년에서 1999년까지 전년 12월에서 4월까지 쏨뱅이 출산기간동안 출산횟수를 조사한 결과 1997년에는 24마리의 어미 중 총 8마리의 어미가 출산에 가입하였고, 이중 5마리는 1회 출산이 이루어 졌으며, 2마리는 2회, 1마리는 3회 출산이 일어났다. 1998년에는 24마리의 어미를 수용하여, 6마리의 어미에서 출산이 이루어 졌으며, 이중에 4마리가 1회만 출산하였고, 각각한 마리가 2회와 4회 출산하였다. 전장 23.6cm(체중 244.7g)의 쏨뱅이 어미는 총 4회 출산하면서 215,000마리의 자어를 나누어 낳았다. 1999년에는 21마리의 암컷 중 11마리가 출산하였다. 이중 9마리가 1회 출산하였으며 2마리가 2회 출산하였다. 종묘생산 된 쏨뱅이를 대상으로 자어 출산을 조사한 결과 만 2년어부터 출산에 가입하고 있었으며 출산기간동안 1회 출산하였다. 2. 출산자어의 마리수. 1997년과 1998년에 각각 24마리, 1999년에 31마리의 쏨뱅이 어미를 이용하여 출산자어 마리수를 조사하였다. 1997년 쏨뱅이 어미(전장 19.2~23.9cm, 체중 150.0~354.0g)에서 1회 출산 할 때마다 18,000~46,000 마리의 자어를 출산하였다 . 마리당 총출산량은 20,000~56,000 마리 였다. 1998년 쏨뱅이 어미 (전장 19.2~24.7cm, 체중 144.3~317.9g)는 1회 출산에 8,000~90,000마리의 자어를 출산하였고, 마리당 총출산량은 8,000~215,000 마리의 자어를 낳아서 개체에 따라 큰 차이를 나타내었다. 1999년에는(어미 : 전장 20.7~25.5cm, 체중 205.5~396.4g) 1 회 출산때마다 7,000~50,000 마리의 자어를 출산하였다. 3. 출산간격. 다회 출산한 쏨뱅이 어미가 첫 번째 출산에서 다음 출산까지 소요되는 기간을 조사한 결과 13일에서 25일이 걸리는 것으로 나타났다. 특히 4회 출산 한 1998년 쏨뱅이 어미는 첫 번째 출산 후 19일만에 두 번째 출산이 이루어 졌고 각각 13 일과 14 일만에 세 번째, 네 번째 출산이 이루어져 총 46일 동안 4회에 거쳐 자어를 출산하였다. 4. 출산자어의 크기. 1997년 3회 출산한 쏨뱅이의 출산시기별 자어 크기를 조사한 결과 첫 번째 출산에서는 평균전장 4.1$\pm$0.07mm의 자어를 낳았으나 두 번째 출산에서 자어의 크기는 평균전장이 3.9$\pm$0.11mm, 세 번째 3.8$\pm$0.12mm로 점차 작은 경향을 나타내었다.

• Korean Journal of Microbiology
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• v.49 no.2
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• pp.205-210
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• 2013

Lumazine protein is a fluorescent protein isolated from the bioluminescent bacteria of Photobacterium species. To generate minimal size of lumazine protein with possessing fluorescent characteristic, the gene coding for the wild type N-terminal domain of lumazine protein (N-LumP 118) containing amino acids up to 118 from Photobacterium leiognathi was produced. In addition, the genes coding for the variant proteins of N-LumP 118, replaced with one tryptophan amino acid (N-LumP 118 V41W, S48W, T50W, D64W, and A66W), were also constructed by Polymerase Chain Reaction and Site Directed Mutagenesis. These proteins were expressed in Escherichia coli by transformation with recombinant plasmids and purified by 6X-His tagging system. Spectroscopic studies have show that the purified proteins are capable of binding to the fluorescent ligand 6,7-dimethyl-8-ribityllumazine, resulted in showing of fluorescent characteristic with the minimal size of protein. From these studies, the mutant proteins containing single tryptophan amino acid residue, possessing its own intrinsic flouophore character at the different position, will be able to the use as a probe for further studies to deduce their three dimensional structure and the binding modes.

• The Journal of Dong Guk Oriental Medicine
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• v.6 no.1
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• pp.205-229
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• 1997

JuJinHyoung(朱震亨) is a well-known physcian of the four schools in the Jin(金) and Yuan(元) Dynasties. He lived in ZhejiangSheng(浙江省) which located in the southeast region of China. There was humid and heatful climate, this kinds of environments influenced to his medical thoughts. He persisted in the theory that Yang is ever in excess while Yin is ever deficient(陽常有餘陰不足論) and exhorted the therapy of subduing excessive Yang and making up deficient Yin by nourishing it, and he said about the theory of ministerial fire(相火論). So he was regarded as a representative of the nourising Yin school(滋陰派). And he said that the method of "Clearing away heat and nourishing blood" is suitable in pregnancy. So Stucellaria baicalensis GEORGI and Atractylodes macrocephala KOIDZ. Can be apply in case of treatment on Anti-abortion. Thus these theories have influenced on Obstetric & Gynecology in Korea.

• Journal of Bio-Environment Control
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• v.7 no.3
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• pp.191-205
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• 1998

Among cauliflower, ‘Snow king’ and ‘Snow crown’, which are early maturing cultivars, showed active early vegetative growth. However, ‘Snow dress’ showed good curd characteristics and in marketability, ‘Green beauty’was superior and can also be grown in Cheju. All the cauliflower and broccoli Plots transplanted on Sep. 3 and on Sep. 17 had fewer number of days from the planting date to the harvesting date than did the plots transplanted on Oct. 1. h the case of broccoli, those transplanted on Oct. 1 had the highest marketability. In the case of cauliflower cultivars, ‘Snow dress’a late maturing cultivar, demonstrated the greatest reduction in growing stage when cultivated in an unheated plastic film houses and broccoli, growing stage is similar between those grown in an open field and those grown in unheated plastic film houses. For all the cauliflower and broccoli, the contents of vitamin A and C showed no significant difference between those grown in an open field and those grown in unheated plastic film houses.

• The Korean Journal of Food And Nutrition
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• v.8 no.3
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• pp.199-205
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• 1995

The study was carried out to compare the factors of overall preference In the sensory test to the analyzation of some compositions in the 7 kinds of old grasses : An Evening Primerose, a Spiderworts, the flower of a Convolvulus, So Ru Jang Yl, Shoe Bl Rum.O Yi Pul, Jip Sean Na Mul. Results were summaries as follows. 1. The Tannin contents of fresh sample and cooked samples were determined as 0.27~2.4g%, 0.25~1.439% respectively. The largest amount of fresh samples was contained In a Shoe Bi Rum. The smallest amount of them was in an Evening Primerose. The highest level of cooked samples was found in a Shoe Bi Rm, and the lowest was in the O Yi Pul. These results were similar to sensory test. 2. The free amino acid contents of 2 kinds of samples were determined as 25.15~179.5mg%, 1.86~13. 6mg% respectively. The largest amount of sweet taste of them was 0 Yi Pul and So Ru Jaeng Yl respectively. But So Ru Jaeng Yi is not appeared sweety becase this have much tannin. The smallest amount of sweet taste was a Spiderwort. The highest level of bitter taste was So Ru Jaeng Yl the and lowest was Jip Sean Na Mul. Among of them Jlp Sean Na Mul is similar to organoleptic test but So Ru Jaeng Yi is not strong bitter taste in sensory evaluation. The highest level of sour taste of cooked samples was So Ru Jaeng Yi and the lowest of them was Shoe Bi Rum. The reducing sugar contents of fresh sample and cooked samples were determined as 1.80~ 4.9g%, 1.84 ~3.579% respectively. The largest of fresh samples were So Ru Jaeng Yl and the lowest was Shoe Bi Rum. The highest of cooked samples were an Evening Primerose and the lowest was a Convolvulus. Among of these results an Evening Primerose was not similar to sensory test because it has much other components. The level of chlorophyll of fresh samples and cooked samples were determined as 11.7~39mg%, 11.3~40.3mg% respectively. The highest of fresh samples was Shoe Bl Rum and the lowest was J ip Sean Na Mul. The largest of cooked samples was So Ru Jaeng Yi and the lowest was a Jlp Seu Na Mul.