• Title/Summary/Keyword: 2-phase model

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Nitric Oxide Exerts Different Functions on Porcine Oocytes Cultured in Different Models, Which is Affected by Beta-mercaptoethanol

  • Tao, Yong;Xia, Guoliang;Bo, Shumin;Zhou, Bo;Zhang, Meijia;Wang, Fenghao
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.3
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    • pp.317-324
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    • 2004
  • The present study was conducted to investigate the involvement of nitric oxide (NO) in cumulus expansion, oocyte mortality and meiotic maturation of porcine cumulus enclosed oocytes (CEOs) cultured in two different models when gonadotropins, including follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG) were presented or not. And the interaction between NO and $\beta$-mercaptoethanol ($\beta$-ME), a free radical scavenger was also investigated. Two models refer to spontaneous maturation model and hypoxanthine (HX) medium model. All the 3,433 eligible CEOs were incubated at $39^{\circ}C$ and the cumulus expansion, oocyte morphology and nuclear phase were evaluated 44 h after incubation. (1) In spontaneous maturation model, NO stimulates the cumulus expansion and $\beta$-ME delayed it. NO doesn't affect the oocyte meiotic resumption but inhibits the oocytes to develop to metaphase II. (2) In HX medium model, NO or $\beta$-ME doesn't affect the expansion in the absence of gonadotropins, but in the presence of gonadotropins, NO or $\beta$-ME inhibits the expansion. In the presence of gonadotropins, NO inhibits the oocyte meiotic resumption and it especially inhibits the oocyte to develop to metaphase II, and $\beta$-ME reverses such inhibitory effects. The cooperation of gonadotropins and $\beta$-ME stimulates the meiotic resumption and especially, promotes the CEOs to develop to metaphase II in both models. Moreover, HX might contribute to the fragility of oocyte zona pellucida and gonadotropins, nitric oxide and $\beta$-ME could alleviate it separately, and cooperatively. It is concluded that NO exerts different functions in two models and $\beta$-ME affected the functions of NO in different models.

Computational Fluid Dynamics(CFD) Simulation for a Pilot-scale Selective Non-catalytic Reduction(SNCR) Process Using Urea Solution (요소용액을 이용한 파일럿규모 SNCR 공정에 대한 CFD 모델링 및 모사)

  • Nguyen, Thanh D.B.;Kang, Tae-Ho;Lim, Young-Il;Kim, Seong-Joon;Eom, Won-Hyeon;Yoo, Kyung-Seun
    • Korean Chemical Engineering Research
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    • v.46 no.5
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    • pp.922-930
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    • 2008
  • The selective non-catalytic reduction(SNCR) performance is sensitive to the process parameters such as flow velocity, reaction temperature and mixing of reagent(ammonia or urea) with the flue gases. Therefore, the knowledge of the velocity field, temperature field and species concentration distribution is crucial for the design and operation of an effective SNCR injection system. In this work, a full-scale two-dimensional computational fluid dynamics(CFD)-based reacting model involving a droplet model is built and validated with the data obtained from a pilot-scale urea-based SNCR reactor installed with a 150 kW LPG burner. The kinetic mechanism with seven reactions for nitrogen oxides($NO_x$) reduction by urea-water solution is used to predict $NO_x$ reduction and ammonia slip. Using the turbulent reacting flow CFD model involving the discrete droplet phase, the CFD simulation results show maximum 20% difference from the experimental data for NO reduction. For $NH_3$ slip, the simulation results have a similar tendency with the experimental data with regard to the temperature and the normalized stoichiometric ratio(NSR).

Growth curve estimates for wither height, hip height, and body length of Hanwoo steers (Bos taurus coreanae)

  • Park, Hu-Rak;Eum, Seung-Hoon;Roh, Seung-Hee;Sun, Du-Won;Seo, Jakyeom;Cho, Seong-Keun;Lee, Jung-Gyu;Kim, Byeong-Woo
    • Korean Journal of Agricultural Science
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    • v.44 no.3
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    • pp.384-391
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    • 2017
  • Growth curves in Hanwoo steers were estimated by Gompertz, Von Bertalanffy, Logistic, and Brody nonlinear models using growth data collected by the Hanwoo Improvement Center from a total of 6,973 Hanwoo (Bos taurus coreanae) steers 6 to 24 months old that were born between 1996 and 2015. The data included three parameters: A, mature size of body measurement; b, growth ratio; and, k, intrinsic growth rate. Nonlinear regression equations for wither height according to Gompertz, Von Bertalanffy, Logistic, and Brody models were $Y_t=144.7e^{-0.5869e^{-0.00301t}}$, $Y_t=145.3(1-0.1816e^{-0.00284t})^3$, $Y_t=143.1(1+0.7356e^{-0.00352t})^{-1}$, and $Y_t=146.8(1+0.4700e^{-0.00249t})^1$, respectively, while those for hip height were $Y_t=144.5e^{-0.5549e^{-0.00312t}}$, $Y_t=145.0(1-0.1724e^{-0.00295t})^3$, $Y_t=143.1(1+0.6863e^{-0.00360t})^{-1}$, and $Y_t=146.2(1+0.4501e^{-0.00263t})^1$, respectively. Equations for body length $Y_t=174.1e^{-0.8342e^{-0.00289t}}$, $Y_t=175.8(1-0.2500e^{-0.00265t})^3$, $Y_t=170.0(1+1.1548e^{-0.00363t})^{-1}$, and $Y_t=180.3(1+0.6077e^{-0.00215t})^1$, respectively, for the same models. Among the four models, the Brody model resulted in the lowest mean square error, with mean square errors of 31.79, 30.57, and 42.13, respectively, for wither height, hip height, and body length. Also, an estimated birth wither height, birth hip height, and birth body length (77.98, 80.57, and 70.97 cm, respectively) were lower in the Brody model than in other models. An inflection point was not observed during the growth phase of Hanwoo steer according to the growth curves calculated using Gompertz, Von Bertalanffy, and Logistic models. Based on the results, we concluded that the regression equation using the Brody model was the most appropriate among the four growth models. To obtain more accurate parameters, however, using data from a wider production period (from birth to shipping) would be required, and the development of a suitable model for body conformation traits would be needed.

A Study on the Application of 3D Virtual Human in the prior stage of Design Works - With the emphasis on the determining design layout of a forklift truck (디자인 초기단계에서의 3차원 가상인간(Virtual Human) 활용에 관한 연구 - 지게차 레이아웃 결정 단계를 중심으로)

  • 김관명
    • Archives of design research
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    • v.12 no.4
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    • pp.294-303
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    • 1999
  • With the breakthrough development of computing environment, the design phases have been changed a lot nowadays. In the case of prior phases of transportation design such as cars and forklift-trucks design, designers have depended on surveys and 2D line drawings for fixing a product layout and extracting ergonomic data. In this method, designers don't meet only the problem of reliability of measuring data but also, the problems of unknown situation of operators' fatigue and comfort in work situation. In these methods, it has much less creditability to have a 2D human model to check the real world motion due to the limitation of the 3 Dimension. Even though with a 2D human model, perfect layout is determined, it is still difficult to measure about comfort and fatigue for a user because it measuring an analysing method is static. The development of computer hardware and software have not only changed the flow in the social-wide range but also immerged design into Virtual Environment. In conventional design method, visualization and data transferring have been the main issues but, in virtual environment, determining of design layout and analysing ergonomic data with sophisticated feeling about comfort and fatigue are possible by using 3D virtual human. In this study, the general characteristics of virtual environment was discussed and the possibility of digital process of design was treated. For these studies, layout design for forklift-trucks was tested. Eventually, the merits of each design phase applied virtual environment are discussed.

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α-Mangostin and Apigenin Induced Cell Cycle Arrest and Programmed Cell Death in SKOV-3 Ovarian Cancer Cells

  • Ittiudomrak, Teeranai;Puthong, Songchan;Roytrakul, Sittiruk;Chanchao, Chanpen
    • Toxicological Research
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    • v.35 no.2
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    • pp.167-179
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    • 2019
  • Ovarian cancer is the fifth main cause of pre-senescent death in women. Although chemotherapy is generally an efficient treatment, its side effects and the occurrence of chemotherapeutic resistance have prompted the need for alternative treatments. In this study, ${\alpha}$-mangostin and apigenin were evaluated as possible anticancer alternatives to the chemotherapeutic drug doxorubicin, used herein as a positive control. The ovarian adenocarcinoma cell line SKOV-3 (ATCC No. HTB77) was used as model ovarian cancer cells, whereas the skin fibroblast line CCD-986Sk (ATCC No. CRL-1947) and lung fibroblast line WI-38 (ATCC No. CCL-75) were used as model untransformed cells. Apigenin and doxorubicin inhibited the growth of SKOV-3 cells in a dose- and time-dependent manner. After 72 hr exposure, doxorubicin was mostly toxic to SKOV-3 cells, whereas apigenin was toxic to SKOV-3 cells but not CCD-986Sk and WI-38 cells. ${\alpha}$-Mangostin was more toxic to SKOV-3 cells than to CCD-986Sk cells. A lower cell density, cell shrinkage, and more unattached (floating round) cells were observed in all treated SKOV-3 cells, but the greatest effects were observed with ${\alpha}$-mangostin. With regard to programmed cell death, apigenin caused early apoptosis within 24 hr, whereas ${\alpha}$-mangostin and doxorubicin caused late apoptosis and necrosis after 72 hr of exposure. Caspase-3 activity was significantly increased in ${\alpha}$-mangostin-treated SKOV-3 cells after 12 hr of exposure, whereas only caspase-9 activity was significantly increased in apigenin-treated SKOV-3 cells at 24 hr. Both ${\alpha}$-mangostin and apigenin arrested the cell cycle at the $G_2/M$ phase, but after 24 and 48 hr, respectively. Significant upregulation of BCL2 (apoptosis-associated gene) and COX2 (inflammation-associated gene) transcripts was observed in apigenin- and ${\alpha}$-mangostin-treated SKOV-3 cells, respectively. ${\alpha}$-Mangostin and apigenin are therefore alternative options for SKOV-3 cell inhibition, with apigenin causing rapid early apoptosis related to the intrinsic apoptotic pathway, and ${\alpha}$-mangostin likely being involved with inflammation.

The Design of Controller and Modeling for Bi-directional DC-DC Converter including an Energy Storage System (에너지 저장장치를 포함하는 양방향 DC-DC 컨버터 모델링 및 제어기 설계)

  • Kim, Seung-Min;Yang, Seung-Dae;Choi, Ju-Yeop;Choy, Ick;An, Jin-Woong;Lee, Sang-Chul;Lee, Dong-Ha
    • Journal of the Korean Solar Energy Society
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    • v.32 no.spc3
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    • pp.235-244
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    • 2012
  • This paper presents a design and simulation of bi-directional DC/DC boost converter for a fuel cell system. In this paper, we analyze the equivalent model of both a boost converter and a buck converter. Also we propose the controller of bi-directional DC-DC converter, which has buck mode of charging a capacitor and boost mode of discharging a capacitor. In order to design a controller, we draw bode plots of the control-to-output transfer function using specific parameters and incorporate proper compensator in a closed loop. As a result, it has increased PM(Phase Margin) for better dynamic performance. The proposed bi-directional DC-DC converter's 3pole-2zero compensation method has been verified with computer simulation and simulation results obtained demonstrates the validity of the proposed control scheme.

System Level Space Environment Testing of Satellite Digital Transponder (디지털 위성중계기에 대한 시스템 단위의 우주환경 검증 시험)

  • Song, Young-Joong;Kim, Jung-Ho;Lee, Sue-Hyun;Seo, Hak-Geum;Shin, Guan-Ho;Jin, Bong-Chul
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38C no.12
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    • pp.1159-1169
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    • 2013
  • EQM Model Digital transponder applying the Korean's own designing and manufacturing technology has gone through a series of trials and errors during the development. In particular, lack of thermal designs expedience for the vacuum causes variety of errors in designing, assembling transponder and setting up the test at the first thermal vacuum test (TVAC). Since the first TVAC test could not accomplished its aims successfully, so the second TVAC should be performed as make up test with revised Digital transponder. In this paper, the defects that identified in the first TVAC are analyzed and applied solutions and its results at the second TVAC are presented. Using the lessons from the first and second TVAC, we will be able to make more reliable digital transponders in the next phase of project. In addition it also be useful as a reference when we design another satellite payloads.

Effects of Torsional Flexibility on a Flapping Airfoil (플랩핑 에어포일에 대한 비틀림 유연성의 영향)

  • Cho, Moon-Sung;Bae, Jae-Sung;Kim, Hark-Bong;Kim, Woo-Jin
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.36 no.12
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    • pp.1146-1151
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    • 2008
  • In this paper, the effects of torsional flexibility on a flapping airfoil are investigated. The aerodynamic forces of a torsional flexible flapping airfoil is computed using 2-D unsteady vortex panel method. A typical-section aeroelastic model is used for the aeroelsatic calculation of the flapping airfoil. Torsional flexibility and excitation frequency are considered as main effective parameters. Under heavy airfoil condition , the thrust peak is observed at the points where the frequency ratio is about 0.75. Based on this peak criterion, there exists two different motions. One is an inertia driven deformation motion and the other is an oscillation driven deformation motion. Also, in the thrust peak condition, the phase angle is kept 85 degrees, independent of the torsional flexibility and the excitation frequency.

Dynamic Analysis and Control Loop Design of ZVS-FB PWM DC/DC Converter (ZVS-FB PWM DC/DC 변환기의 동특성 해석 및 제어기 설계)

  • 이득기;윤길문;차영길;김흥근
    • The Transactions of the Korean Institute of Power Electronics
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    • v.3 no.3
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    • pp.231-239
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    • 1998
  • This paper presents the dynamic analysis and control loop design of a zero voltage switching full bridge (ZVS-FB) PWM DC/DC converter. The small-signal model is derived incorporating the effects of phase shift control and the utilization of transformer leakage inductance and power FET junction capacitance to achieve zero voltage resonant switching. These effects are modeled by introducing additional feedforward and feedback terms for duty cycle modulation. Based on the results of the small-signal analysis, the control loop is designed using a simple two-pole one-zero compensation circuit. To show the validity of the design procedures, the small signal analysis of the closed loop system is carried out and the potential of the zero voltage switching and the superiority of the dynamic characteristics are verified through the experiment with a 2 kW prototype converter.

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Estimation of Reservoir Discharge to Support TMDL Management in the Geum River Basin (금강수계 오염총량관리를 고려한 저수지 방류량산정)

  • Noh Joon-Woo;Kim Soo-Jun;Kim Jeong-Kon;Koh Ick-Hwan
    • Journal of Korea Water Resources Association
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    • v.39 no.7 s.168
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    • pp.627-636
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    • 2006
  • This study estimates adequate discharge to meet the specified target water quality concentration using the pollutant load of the Geum river basin given in TMDL (Total Maximum Daily Load) report. During the 1st phase, BOD is chosen as a target water quality constituent under regulation of the Ministry of Environment in Korea. BOD, TN, and TP loads estimated based on the TMDL and provincial zones were re-distributed for 10 major tributaries, and the remaining areas along the main river are classified as 15 incremental flow areas. Water quality modeling was conducted using Qual2E for the low flow period of a year (i.e. $March{\sim}April$). The results of the model simulation showed that about 30 cms from the Daechung dam would be sufficient to satisfy the target water quality in the Geum river downstream of the Daechung multipurpose Dam.