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The Effects of Antioxidant Vitamins Via Comet and Mitogenic Assay in Mice Exposed to $AFB_1$ ($AFB_1$에 노출된 마우스에서 Comet Assay와 Mitogenic Assay에 의한 항산화 비타민의 효과)

  • Park, Seon-Ja
    • Journal of Korean Biological Nursing Science
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    • v.3 no.2
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    • pp.21-33
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    • 2001
  • The objective of this study was to examine the effects of antioxidant vitamins on the cellular oxidant damage by observing the mitogenicity in the mouse spleen and the strand breaks of DNA in mouse blood induced by $AFB_2$. Intraperitoneal(i.p.) injections of vitamin C(VC) of 10 mg/kg and vitamin E(VE) of 63.8 mg/kg were repeatedly administered to male ICR mice of 6 weeks old at intervals of 4 times every 2 days. After one hour vitamin treatments, $AFB_1$ of 0.4 mg/kg was injected into the $AFB_2$ plus vitamin treated groups in the same way. On the other hands, into the $AFB_2$ only treated group, only $AFB_2$ was injected without vitamins in the same method as above. The results of the experiment are as follows ; as regard to comet assay, DNA strand breaks were clearly present and they formatted a typical comet tail in the mice blood of the $AFB_2$ only treated groups. However, comet tails apparently disappeared in $AFB_2$ plus antioxidant vitamins treated groups since oxidant damage was controlled in an almost similar level to the control group. Mitogenicity of the spleen also showed a similar tendency as before, and these differences were more remarkably observed in the reaction against Con-A, which is a T-cell mitogen. In these data, the statistical significance was p<0.01. The LDL and VLDL levels were 408.72, 504.47 mg/dl respectively in the $AFB_2$ only treated groups. Compared with the $AFB_1$ only treated groups, those of $AFB_2$ plus antioxidant vitamin treated groups decreased to 272.06(VC), 305.28 mg/dl(VE), respectively. On the other hand, HDL levels were diminished to 32.60, 29.60 mg/dl in $AFB_2$ only treated groups, compared to 42.23, 41.14 mg/dl in the $AFB_2$ plus antioxidant vitamins treated groups. But, blood glucose levels were not statistically significant.

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IDENTITIES ARISING FROM GAUSS SUMS FOR SYMPLECTIC AND ORTHOGONAL GROUPS

  • Chae, Hi-Joon;Kim, Dae-San
    • Journal of the Korean Mathematical Society
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    • v.47 no.2
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    • pp.409-424
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    • 2010
  • We express Gauss sums for symplectic and orthogonal groups over finite fields as averages of exponential sums over certain maximal tori. Together with our previous results, we obtain some interesting identities involving various classical Gauss and Kloosterman sums.

An Experimental Study on Effects of Distilled Red-ginseng Herbal Acupuncture on A549 human ephithelial lung cancer cell in vitro and implanted Sarcoma-180 (A549 폐암세포와 Sarcoma-180 복강암에 대한 홍삼(紅蔘) 증류약침(蒸溜藥鍼)의 영향에 관(關)한 실험적(寶驗的) 연구(硏究))

  • Won, Seung-Hwan;Kwon, Ki-Rok;Lee, Sun-Gu
    • Journal of Pharmacopuncture
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    • v.7 no.2
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    • pp.43-56
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    • 2004
  • Objectives : In order to investigate effects and immune improvement of distilled red-ginseng herbal Acupuncture, expression of Cox-1, Cox-2, and mRNA of Bcl-2 and Bax were analyzed in A549 cell in vivo. Survival time and expression of cytokine mRNA were measured for the mice with Sarcoma-180 induced abdominal cancer. Methods : Balb/c mouse was treated with distilled red-ginseng Herbal Acupuncture at Wisu($BL_{21}$) and Chung- wan($CV_{12}$) to investigate anti-cancer effects and immune response. Results : 1. For expression of mRNA of Cox-1 using RT-PCR, the control group and the experiment groups didn't show significant differences. For Cox-2, both experiment groups and the normal group showed significant differences. 2. For expression of mRNA of Bcl-2 using RT-PCR, experiment groups showed slight decrease compared to the control group. For Bax, no significant changes were shown between the control group and experiment groups. 3. For survival time, all of experiment groups showed 11.1% increase compared to the control group. 4. For IL-2 and IL-4 productivity using Flow cytometry, all of experiment groups didn't show any significance. 5. For IL-2 productivity using ELISA, all of experiment groups didn't show any significance. 6. For expression of cytokine mRNA using RT-PCR, significant increase of IL.-2 and IL-4 were witnessed in the experiment group II compared to the control group. Significant increase of IL-10 was shown in all off experiment groups compared to the control group. Conclusion : According to the results, we can expect that distilled red-ginseng Herbal Acupuncture may be further effccts in anti-cancer and immune improvement if increasing concentration.

The Activities of Agricultural Products Processing and Future Strategies by Rural Women Groups in Japan (일본농촌여성의 농산물가공활동과 발전가능성 - 효고현(兵庫懸)의 농촌여성가공회를 중심으로 -)

  • 노채영
    • Korean Journal of Human Ecology
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    • v.2 no.1
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    • pp.68-79
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    • 1999
  • Investigations were made on the status of women groups on processing activities of agricultural products for the Hyogo prefecture in Japan. The data were analyzed with the Hideyosi for Windows(Version 1.19) and the Econometric View for Quantative Micro Softwere(Version 2.0). The results indicats that many rural women groups have their own experience on the activities of processing agricultural products and many other women groups have gained such experience from the management and establishment of the restaurants and direct sales stores. This kind of systems has influenced the activation of each rural district. Further development of the processing of agricultural products by rural women groups in the future demands better cooperation among diverse groups involved in these establishment centers and consumers. In addition, it is necessary to attract young people and train and upgrade them for successive consumers. (Korean J of Human Ecology 2(1) : 68∼79, 1999)

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The Influence of Protecting Groups on the β-Sheet Structure Stability of Protected Peptides

  • 이진식;이동진
    • Bulletin of the Korean Chemical Society
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    • v.16 no.7
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    • pp.591-594
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    • 1995
  • The influence of protecting groups on the β-sheet-structure-stability of protected peptides was studied in organic solvents. α-amino groups, carboxyl groups and side chain functional groups of model peptides were protected by suitable groups commonly used in peptide synthesis. The difference of the solubilities of model peptides was investigated by the solvent-titration method by using IR absorption spectra. The β-sheet structure of model peptide in CH2Cl2 was easily disrupted by increasing the amounts of DMSO. The β-sheet-structure-stabilizing potentials of each protecting group showed similar behaviors except Npys, Mts and Z2. The result exhibits that the < SPβ > values of protected peptides are almost independent of the kinds of their protecting groups.

THE EFFCT OF TIME DELAY AFTER SILICOATING ON THE BOND STRENGTH BETWEEN THE METAL AND RESIN CEMENT (Silicoating후 시간지연이 금속과 레진 시멘트 간의 접착강도에 미치는 영향)

  • Yang, Jin-Kyoung;Bae, Jeong-Sik
    • The Journal of Korean Academy of Prosthodontics
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    • v.33 no.1
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    • pp.1-12
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    • 1995
  • For the purpose of this study, the silicoating on Ni-Cr-Be alloy surface was carried out by using the Silicoater MD. The effect of time delay after silicoating on the bond strength between the metal and resin cement was examined. The groups were divided into 4 : group 1 : Sandblasted with $250{\mu}m$ aluminium oxide, immediatly cemented group 2 : Sandblasted with $250{\mu}m$ aluminium oxide, silicoated and immediatly cemented group 3 : Sandblasted with $250{\mu}m$ aluminium oxide, silicoated and cemented after 7 days group 4 : Sandblasted with $250{\mu}m$ aluminium oxide, silicoated and coated with unfilled resin and cemented after 7 days Specimens were luted with Comspan, Superbond C&B or Panavia EX. The tensile bond strength between the metal and resin cement was measured by using the universal testing machine. The results were as follows : 1. The bond strength in groups 2, 3 and 4 was higher than that of group 1 (p<0.05). There was no statistically significant difference among the bond strength in groups 2, 3 and 4 (p>0.05). 2. In Comspan-cemented groups, there was no significant difference among the bond strength in groups 2, 3 and 4 (p<0.05). 3. In Panavia EX-cemented groups, the bond strength of group 3 was higher than that of group 2 (p<0.05) and there was no significant difference between groups 3 and 4. 4. In Superbond C&B-cemented groups, there was significant difference between groups 2 and 3 (p<0.05) and the bond strength of group 4 was higher than that of group 3 (p<0.05). 5. The highest tensile bond strength was obtained by using the Superbond C&B and no difference in the Panavia EX and Comspan. 6. The modes of bond failure were mainly cohesive failure. The method of storage and transport indicated in this study seems recommendable for laboratory and clinical use at least up to 7 days.

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Effects of the Feeding Methods on Body Growth and Sexual Maturity in Broiler Breeder Males (육용종웅계의 사양방법이 체성장 및 성 성숙에 미치는 영향)

  • 연성흠;이규승;정일정;정선부
    • Korean Journal of Poultry Science
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    • v.13 no.2
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    • pp.197-208
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    • 1986
  • This experiment was conducted to find out the effects of the six different feeding methods on the development of body weight, testis, comb and pituitary gland, and the sexual maturity of White Plymouth Rock cockerels. From hatching to 22 weeks of age, the weights of whole body, testis, comb and pituitary gland, and the histological changes of testis and the semen characteristics were checked every other week. The results obtained in this expeniment were as follows: 1. The growth rates of the self-feeding groups were faster than those of the limited feeding groups (70 percent of the self-feeding) by about 2 weeks. The weights of testis and comb showed the most marked increase at 20 weeks of age in the self-feeding groups and at 22 weeks of age in the limited feeding groups, respectively. 2. The weights of pituitary gland from hatching to 22 weeks of age at all observation weeks were not recognised significantly among the compared groups except 4, 14 and 16 weeks of age. 3. Correlations between week of age, body weight, testis, comb and pituitary gland, in the course of 22 weeks, were highly significant. 4. The diameters of lumina and tubules in the seminiferous tubules increased very slowly until 10 weeks of age. They showed the most marked increase at 12 weeks of age in the self-feeding groups and at 14 weeks of age in the limited feeding groups, and then continuously increased until 32 weeks of. age. 5. Primary spermatocytes appeared at first at 8 weeks in the all treatment groups, Secondary spermatocytes appeared at first at 10 weeks in the self-feeding groups and at 12 weeks in the limited feeding groups. At 14 weeks of age spermatids and spermatozoa were found at first in the self-feeding groups but spermatids were found in the limited feeding groups. 6. Age of the first ejaculation was between 14 and 16 weeks of age in the all treatment groups. The Average semen. volume and sperm concentration ranged from 0.1-0.2$m\ell$/ ejaculate and 5.6-9.8${\times}$10$\^$8/ sperm/$m\ell$ at the age of the first ejaculation but 0.30-0.35$m\ell$/ ejaculate and 22.4-42.7${\times}$10$\^$8/ sperm int at the 20 weeks of age in the all treatment groups.

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Quality Changes of Sulgiduk Added Green Tea Powder during storage (가루녹차를 첨가한 설기떡이 저장중 품질 변화)

  • 이순재;홍희진;최정화;최경호;최상호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1064-1068
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    • 1999
  • This study was quality changes of sulgiduk added green tea powder during storage. Sulgiduk prepared with various concentrations of green tea powder; 0%(control group), 0.5%(GT 0.5 group), 1%(GT 1.0 group), 1.5%(GT 1.5 group), 2%(GT 2.0 group), and the changes of its quality during 7 days of storage were investigated. Total microbe numbers, the acidity and pH in sulgiduk during storage were decreased with increasing the added amounts of a green tea powder, and especially those of GT 1.0 and GT 1.5 groups had relatively the lower than other groups. The "L" value(lightness) of the control group was the highest among five groups, and its value was decreased throughout storage, and especially GT 0.5 groups had the lowest brightness. The "a" value(reddness) of the control group was higher than other four groups, and increased GT 2.0>GT 1.5>GT 1.0>GT 0.5 groups, in that order. The "b" value(yel lowness) was increased in the order; GT 2.0>GT 1.5>GT 1.0>GT 0.5 groups. These results indicated that GT 1.0 group showed the best quality than other groups of sulgiduk during storage. of sulgiduk during storage.

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Prevention of Adhesion Formation by Use of Carboxymethylcellulose and Ibuprofen in Rats (쥐에서 Carboxymethylcellulose 및 Ibuprofen을 이용한 유착형성 방지에 관한 연구)

  • Choi Min-Cheol;Lee Hyo-Jong;Kim Gon-Sup
    • Journal of Veterinary Clinics
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    • v.10 no.2
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    • pp.203-214
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    • 1993
  • With the use of a rat surgical model, the ability of carboxymethylcellulose and ibuprofen in the reduction of abdominal adhesion was examined. Seventy seven female rats were randomly divided into 7 groups : (1) control, (2) 2% CMC, (3) 3% CMC, (4) ibuprofen 25mg, (5) ibuprofen 50mg, (6) combination of ibuprofen 25mg and 2% CMC and(7) combination of ibuprofen 50mg and 3%, CMC. Following induction of abrasion injuries on ileum, colon and both uterine horns with a surgical blads, the rats in groups (2), (6) were infused with 2% CMC solution singly or in combined Infection of 25 mg/kg of ibuprofen for three consecutive days, the rats In groups (3), (7) were infused with 3% CMC solution singly or In combined Injection of 50mg/kg of ibuprofen for three consecutive days. The rats in groups (4), (5) were injected only with 25 mg or 50 mg/kg of ibuprofen for three consecutive days. After 10 days the abdominal cavities were opened and the appearance of formed adhesion were graded. The changes of body weight, CBC and blood chemicals were also evaluated at 3, 6 and 10 days after operation. In ileum, the rats in the groups (2), (6) and (7) showed less adhesion formation. In colon, there were significant differences(p<0.05) in adhesion formation in all treated groups as compared to control. In both uterine horns, there were significant decrease(p<0.05) of adhesion formation in groups(2), (6) and (7) in comparison with other groups. The increasing rate of body weight was evident in group (3) and fibrinogen concentrations at 6 and 10 days revealed significant decrease (p<0.01) in group (7), whereas there was no consistent change in CBC and blood chemicals. Therefore, it can be sugested that the infusion of 2% CMC solution with or without the injection of 25 mg/kg of ibuprofen and 3% CMC solution with the injection of 50 mg/kg of ibuprofen are effective and safe following abdominal surgery,

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A Randomized Study, Double-Blind, Placebo-Controled Study to Herbal Shampoo & Essence about Dandruff (한방 샴푸/에센스의 비듬에 대한 무작위배정, 이중맹검, 위약 대조군 연구)

  • Kim, Chagn-Hun;Hwang, Dong-Sung;Kim, Jung-Tae;Jung, Hyeon-A;Roh, Seok-Seon;Lim, Nam-Kyoo
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.20 no.3
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    • pp.222-235
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    • 2007
  • Background : Dandruff is a chronic scalp condition characterized by scaling. The common causative agent is now accepted to be the lipophilic Pityrosporum ovale. Obstecive : We conducted a randomized, double-blind, parallel-group study to investigate the efficacy of Herbal Shampoo & Essense and placebo in patients with mild to moderate dandruff. Mathods : Forth-seven patients with dandruff were assigned to one of two treatment goups: Test (contaning the medication) or control (vehicle). After administering through randomizion, two groups were used with Negative sampoo for first two weeks, and used with test or control products for second two weeks. Results : 1. After 2 weeks of treatment, the numbers of P. ovale in test groups were decreased in a statistically meaningful way(p<0.01) compared with control groups. 2. After 2 weeks of treatment, average of sebum-amount in test groups were decreased from 117.55 to 92.88, in a statistically meaningful way(P<0.05, paired t-test) compared with control groups that were decreased from 115.53 to 112.46. 3. In clinical score, average of control groups is decreased from 2.82 to 1.09. on the other sides, average of test groups is decreased from 3.88 to 2.44. The change of test groups is bigger than that of control groups, both two groups was decreased in a statistically way(<0.01) Conclusion : The study demonstrated that Herbal Shampoo & Essense can be used by patients with scalf safely and effectively with almost no side-effect.

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