• Title/Summary/Keyword: 2-crossbred

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Detecting Positive Selection of Korean Native Goat Populations Using Next-Generation Sequencing

  • Lee, Wonseok;Ahn, Sojin;Taye, Mengistie;Sung, Samsun;Lee, Hyun-Jeong;Cho, Seoae;Kim, Heebal
    • Molecules and Cells
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    • v.39 no.12
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    • pp.862-868
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    • 2016
  • Goats (Capra hircus) are one of the oldest species of domesticated animals. Native Korean goats are a particularly interesting group, as they are indigenous to the area and were raised in the Korean peninsula almost 2,000 years ago. Although they have a small body size and produce low volumes of milk and meat, they are quite resistant to lumbar paralysis. Our study aimed to reveal the distinct genetic features and patterns of selection in native Korean goats by comparing the genomes of native Korean goat and crossbred goat populations. We sequenced the whole genome of 15 native Korean goats and 11 crossbred goats using next-generation sequencing (Illumina platform) to compare the genomes of the two populations. We found decreased nucleotide diversity in the native Korean goats compared to the crossbred goats. Genetic structural analysis demonstrated that the native Korean goat and cross-bred goat populations shared a common ancestry, but were clearly distinct. Finally, to reveal the native Korean goat's selective sweep region, selective sweep signals were identified in the native Korean goat genome using cross-population extended haplotype homozygosity (XP-EHH) and a cross-population composite likelihood ratio test (XP-CLR). As a result, we were able to identify candidate genes for recent selection, such as the CCR3 gene, which is related to lumbar paralysis resistance. Combined with future studies and recent goat genome information, this study will contribute to a thorough understanding of the native Korean goat genome.

Effects of Gas Composition in the Modified Atmosphere Packaging on the Shelf-life of Longissimus dorsi of Korean Native Black Pigs-Duroc Crossbred during Refrigerated Storage

  • Muhlisin, Muhlisin;Panjono, Panjono;Kim, Dong Soo;Song, Yeong Rae;Lee, Sung-Jin;Lee, Jeong Koo;Lee, Sung Ki
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.8
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    • pp.1157-1163
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    • 2014
  • This study was conducted to observe the effects of gas composition in modified atmosphere packaging (MAP) on the shelf-life of Longissimus dorsi of Korean Native Black Pigs-Duroc Crossbred ($KNP{\times}D$) during refrigerated storage. Muscle sample was obtained from the left side of carcass of seven months old of $KNP{\times}D$ barrow. The sample was sliced into 1 cm in thickness, placed on trays (two slices/tray) and filled with different gas composition, i.e. 0:20:80/$O_2:CO_2:N_2$ (MAP1), 30:20:50/$O_2:CO_2:N_2$ (MAP2) and 70:20:10/$O_2:CO_2:N_2$ (MAP3). Other slices of sample were vacuum packed (VP) as a control. All packs were stored at $5{\pm}1^{\circ}C$. At 12 d of storage, pH value of MAP2 and MAP3 were higher (p<0.05) than that of MAP1 and pH value of MAP1 was higher (p<0.05) than that of VP. At 6 d of storage, redness ($a^*$) value of MAP2 and MAP3 were higher (p<0.05) than that of VP and MAP1 and, at 9 and 12 d of storage, redness value of MAP3 was higher (p<0.05) than that of VP, MAP1, and MAP2. At 3, 6, 9, and 12 d of storage, the 2- thiobarbituric acid reactive substances (TBARS) value of MAP3 was higher than that of MAP2 and TBARS value of MAP2 was higher than that of VP and MAP1. At 3, 6, 9, and 12 d of storage, volatile basic nitrogen values of MAP2 and MAP3 were higher (p<0.05) than those of VP and MAP1. At 3 d of storage, total aerobic plate counts of MAP2 and MAP3 were higher (p<0.05) than those of VP and MAP1 and, at 6 d of storage, total aerobic plate counts of MAP3 was higher (p<0.05) than that of MAP1 and MAP2. However, there was no significant different total aerobic plate count among MAP1, MAP2, and MAP3 at 9 and 12 d of storage. There was no significant different total anaerobic plate count among MAP1, MAP2, and MAP3 during storage. It is concluded that the MAP containing 30:20:50/$O_2:CO_2:N_2$ gas composition (MAP2) might be ideal for better meat quality for $KNP{\times}D$ meat.

A Study on Improvement of Dual Purpose Breeder Stocks - Selection of Superior Fowl by Means of Cross Breeding - (겸용종계 개량에 관한 연구 - 우량 교배조합선발시험 -)

  • 상병찬;김동곤;박상문;한성욱
    • Korean Journal of Poultry Science
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    • v.6 no.2
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    • pp.75-81
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    • 1979
  • This study was carried out to select superior dual purpose hens which could spread to poultry farming for subsidiary work and general farmhouse. Three hundred and fifty chicks of 7 kinds of 2-way crossbred from mating of Rhode Island Red, New Hampshire and Australorps were tested for dual purpose performance. The results obtained were as follows: 1. The average viability of brooding, rearing and laying Period hen were 92.3% 98.3% and 93.1% respectively. There was significant difference at 1% level among crossbreds for brooding viability, but other viabilitys were no significant difference. 2. The average body weight of 8 weeks was 854.41g, but there was no significant difference among crossbreds, respectively. Average body weight at 300 days, 500 days were 2507.15g, 2632.25g respectively, but there was significant difference at 1% level among crossbreds. 3. Sexual maturity of individual and 50% egg production were 173.7 days and 199.4 days, respectively. There was significant difference at 5% level among crossbreds. 4. The average hen day egg production was 65.3% and the highest was R.N crossbred (70.5%). There was significant difference at 5% level among crossbreds and average hen housed egg Production was 20.2 eggs and the highest was 220.4 eggs in R.A. crossbred. 5. The average egg weight was 57.36g and the highest was A.N crossbred (58.3g). Therc was no significant difference among crossbreds. 6. The average feed consumption per hen per day by stag. was 42.9g (brooding period), 83.7g (rearing period), 131.1g (laying period). Average feed requirement per 1kg. egg production was 3.79 and th. R.A. crossbred was superiority (3.42). 7. On the basis of the results of this study, the R.A and R.N crossbreds were best for superior dual commercial chicks under farming management conditions.

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Development of a Device for Estimating the Optimal Artificial Insemination Time of Individually Stalled Sows Using Direct-reflex Photo-sensors (직접반사형 포토센서를 이용한 스톨 사육 모돈의 인공수정적기 예측 장치 개발)

  • Kim D. J.;Yeon S. C.;Chang H. H.
    • Journal of Biosystems Engineering
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    • v.29 no.6 s.107
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    • pp.515-520
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    • 2004
  • A device far estimating the optimal artificial insemination time consisted of a computer, RS module, and six direct-reflex photo-sensors. Program was written in LabWindows CVI. In order to establish references for estimating optimal artificial insemination time, lying rate of the thirty three $Berkshire{\times}Hampshire crossbred sows (parity 5 to 6) was recorded from 2 days after moving into stalls to artificial insemination using a time lapse VCR and was measured every hour with one minute interval using the scan point sampling method. Twenty low hours in a day were divided into three comparing periods : 21:00 to 06:59, 07:00 to 13:59, and 14:00 to 20:59. If sum of the percentages of tying during a comparing period was less than the reference value, the starting hour of the comparing period was considered as onset of estrus and the optimal artificial insemination time was estimated at 20 to 24 hours after onset of estrus. The experimental device was evaluated with twenty five $Berkshire{\times}Hampshire$ crossbred sows (parity 2 to 7) and 23 of 25 sows $(92\%)$ were pregnant. This result suggests that the experimental device is excellent in performance for estimating optimal artificial insemination time.

Performance of Crossbred Cattle Fed Chopped Green Sugarcane Tops and Supplemented with Wheat Bran or Lentil Chuni Concentrates

  • Gendley, M.K.;Singh, P.;Garg, A.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.10
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    • pp.1422-1427
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    • 2002
  • Fifteen crossbred cattle bulls of about 22-24 months age (mean body weight ranged from 291.0 to 298.1 kg) were randomly divided into 3 groups of 5 animals following randomized block design. Animals in group 1 were fed concentrate mixture containing wheat bran 97%, mineral mixture 2% and salt 1% plus ad libitum chopped green sugarcane tops (SCT) as a roughage source. Animals in group 2 were fed concentrate mixture containing lentil chuni 97%, mineral mixture 2% and salt 1% while group 3 concentrate contained wheat bran 48.5%, lentil chuni 48.5%, mineral mixture 2% and salt 1% plus ad libitum chopped green SCT to meet their maintenance requirements. During metabolism trial period, the mean total intake and digestibility of DM in groups 1, 2 and 3 was $8.09{\pm}0.41$, $8.41{\pm}0.49$ and $7.86{\pm}0.16kg/d$ and 60.24, 63.24 and 65.05%, respectively. The intake of CP (p<0.05) and EE (p<0.01) was significantly higher in group 2 and group 1, respectively as compared to remaining 2 groups, which were comparable. Digestibility of CP and EE was significantly (p<0.01) higher in group 3 and that of CP was comparable with group 2. The intake and digestibility of total carbohydrates, NDF, ADF, Hemi-cellulose and cellulose were similar among 3 groups. The mean body weight changes in group 1, 2 and 3 were 173.4, 253.4 and 203.4 g/d, respectively, which was significantly (p<0.05) higher in group 2. The total nitrogen (N) and phosphorus (P) intakes were significantly higher in group 2 and group 1, respectively. The total volatile fatty acids, ammonia-N, trichloro-acetic acid precipitable nitrogen (TCA ppt.-N; p<0.05), total-N concentrations and pH were significantly (p<0.01) higher in group 3, however, pH and TCA ppt.-N was comparable with group 2 and 1, respectively. From the results, it may be deduced that the feeding of chopped green SCT supplemented with a concentrate mixture having both wheat bran (48.5%) and lentil chuni (48.5%) has shown a better performance with respect to intake and digestibility of nutrients and growth as well.

Effect of Level of Feeding on the Performance of Crossbred Cows during Pre- and Post-partum Periods

  • Singh, Jatinder;Singh, Balwant;Wadhwa, M.;Bakshi, M.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1749-1754
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    • 2003
  • The study was undertaken to see the effect of elevated feeding during pre-partum or pre- as well as post-partum period on the productive and reproductive performance of crossbred cows. The experiment lasted for 60 d pre-partum to 120 d post-partum. Eighteen dry pregnant crossbred cows divided into three equal groups were fed either as per NRC feeding standard (C) or 20% above NRC during 60 d pre-partum ($T_1$) or fed 20% above NRC during both 60 d pre-partum to 120 d post-partum ($T_2$) period. During prepartum period body weight gain was significantly ($p{\leq}0.05$) higher in $T_1$ and $T_2$ groups than that of control group. The animals fed at higher plane of nutrition ($T_1$ and $T_2$) took significantly lesser time for complete relaxation of pelvic muscles, act of calving and for expulsion of placenta than that of control group. Moreover, such cows delivered 2 to 3 kg heavier calves as compared to normal fed dams. During post-partum period, the average daily milk yield was significantly higher in $T_2$ group than that in $T_1$ and control groups. The peak yield was significantly higher in $T_2$ group, it took longer time to reach peak production but it was more persistent in this group as compared to $T_1$ and control groups. Average milk fat, solids-not-fat (SNF) and total solids were significantly higher in $T_1$ and $T_2$ groups as compared to control group. Body weight losses incurred during early lactation were not even compensated by end of 4th month of lactation in C and $T_1$ groups whereas the animals in $T_2$ group gained 2.0 kg. The 1st post-partum estrus and conception rate were better in high fed groups ($T_1$ and $T_2$) than that of control group. The returns over feed cost of milk production were higher in $T_2$ group followed by $T_1$ and control groups indicating the advantage of elevated feeding during pre- and post-partum periods.

Effect of Feeding Graded Levels of Undegraded Dietary Protein on Voluntary Intake, Milk Production and Economic Return in Early Lactating Crossbred Cows

  • Chaturvedi, O.H.;Walli, T.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.8
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    • pp.1118-1124
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    • 2001
  • Fifteen lactating crossbred cows were randomly allotted to three groups of 5 each, and fed three isoproteinous concentrate mixture varying in RDP and UDP ratios, viz. 71: 29 (T1) and 58: 42 (T2) and 44: 56 (T3), along with green maize and wheat straw given as 2/3 and 1/3 of total roughage respectively, for a period of 120 days. The DM intake (kg/d) differed significantly (p<0.01) among the treatments as well as among the fortnights over a period of 120 days. DMI (kg/d) progressively increased from first to eighth fortnight in all the treatments. The daily DMI (% BW) was significantly (p<0.01) lower in T1 (2.37) than those of T2 (2.82) and T3 (2.67). The body weights of cows decreased up to 4th fortnight in T1 and up to 3rd fortnight in T2 and T3, then it showed an increasing trend till the end of the experiment in all the treatments. Cows in T1 lost 10 kg body weight but cows in T2 and T3 gained 23 and 12 kg the body weight, respectively. Both the milk and FCM yield differed significantly (p<0.01) among the fortnights. The FCM yield increased up to 2nd fortnight in all the treatments and thereafter, the FCM yield declined gradually as the lactation advanced. The FCM yield (kg/d) was significantly (p<0.05) higher in T3 (10.47) than in T2 (9.81) and T1 (9.68), however, milk yield, SCM yield and milk energy yield did not differ among the treatments as well as among the fortnights. Fat and protein % in milk increased as the lactation advanced. However, fortnightly SNF % in milk showed an irregular trend. The % fat, protein, SNF and total solids in milk differed significantly (p<0.01) among the fortnights. The % fat and protein in milk varied significantly (p<0.01) among the treatments, being lowest in T1 and highest in T3. The feed efficiency for milk production showed a non-significant variation among the treatments as well as among the fortnights, but increased with the increase in UDP level. It is concluded that by increasing the UDP level from 29 to 56 per cent of CP in the diet of medium producing cows, the milk production increases and cost of milk production reduces.

Effects of Sex and Breed on Meat Quality and Sensory Properties in Three-way Crossbred Pigs Sired by Duroc or by a Synthetic Breed Based on a Korean Native Breed

  • Kim, Yong Min;Choi, Tae Jeong;Cho, Kyu Ho;Cho, Eun Seok;Lee, Jung Jae;Chung, Hak Jae;Baek, Sun Young;Jeong, Yong Dae
    • Food Science of Animal Resources
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    • v.38 no.3
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    • pp.544-553
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    • 2018
  • This study was conducted to determine the effects of breed and sex on meat quality and sensory properties of the loin in three-way crossbred pigs: $Landrace{\times}Yorkshire{\times}Duroc$ (LYD) and $Landrace{\times}Yorkshire{\times}Woori$ (LYW) black pig synthesized by Korean native breed. Carcass traits did not differ by breed. Carcass weight and backfat thickness were higher in castrates than in gilts (p<0.01). LYW showed significant high values in fat content, cooking loss, and water-holding capacity (WHC) than LYD (p<0.05). Redness and yellowness of the meat were higher in LYW than in LYD (p<0.01). Further, LYW had lower pH and shear force than LYD (p<0.001). Significant high scores in color and flavor were obtained in LYW or gilts compared to LYD or castrates by sensory panel, respectively (p<0.05). However, other sensory traits did not differ by breed or sex. Capric acid (C10:0) was higher in LYD than LYW (p<0.001). However, stearic acid (C18:0) and saturated fatty acid (SFA) contents were higher in LYW than LYD (p<0.05). Eicosenoic acid (C20:2) and the n6/n3 ratio were higher in gilts than in castrates, whereas SFA content was higher in castrates than in gilts. These results suggest that certain physicochemical qualities of meat and sensory properties are improved in LYW compared to LYD. This study could provide basic data on meat quality of crossbred pigs with Woori black pig as a terminal sire.

Evaluation of coat color inheritance and production performance for crossbreed from Chinese indigenous Chenghua pig crossbred with Berkshire

  • Li, Yujing;Yuan, Rong;Gong, Zhengyin;Zou, Qin;Wang, Yifei;Tang, Guoqing;Zhu, Li;Li, Xuewei;Jiang, Yanzhi
    • Animal Bioscience
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    • v.35 no.10
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    • pp.1479-1488
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    • 2022
  • Objective: This work was to determine coat inheritance and evaluate production performance for crossbred pigs from Berkshire×Chenghua (BC) compared with Chinese indigenous Chenghua (CH) pigs. Methods: The coat color phenotypes were recorded for more than 16,000 pigs, and the genotypes of melanocortin 1 receptor (MCIR) gene were identified by sequencing. The reproductive performance of 927 crossbred BC F4 gilts and 320 purebred CH gilts was recorded. Sixty pigs of each breed were randomly selected at approximately 60 days of age to determine growth performance during fattening period, which lasted for 150 days for BC pigs and 240 days for CH pigs. At the end of the fattening period, 30 pigs of each breed were slaughtered to determine carcass composition and meat quality. Results: The coat color of BC pigs exhibits a "dominant black" hereditary pattern, and all piglets derived from boars or sows genotyped ED1 ED1 homozygous for MC1R gene showed a uniform black coat phenotype. The BC F4 gilts displayed a good reproductive performance, showing a higher litter and tear size and were heavier at farrowing litter and at weaning litter than the CH gilts, but they reached puberty later than the CH gilts. BC F4 pigs exhibited improved growth and carcass characteristics with a higher average daily live weight gain, lower feed-to-gain ratio, and higher carcass lean meat rate than CH pigs. Like CH pigs, BC F4 pigs produced superior meat-quality characteristics, showing ideal pH and meat-color values, high intramuscular fat content and water-holding capacity, and acceptable muscle-fiber parameters. C18:1, C16:0, C18:0, and C18:2 were the main fatty acids in M. longissimus lumborum in the two breeds, and a remarkably high polyunsaturated/saturated fatty acid ratio of ~0.39 was observed in the BC F4 pigs. Conclusion: The BC F4 pigs exhibit a uniform black coat pattern and acceptable total production performance.

Effect of Pre-partum Feeding of Crossbred Cows on Growth Performance, Metabolic Profile and Immune Status of Calves

  • Panigrahi, B.;Pandey, H.N.;Pattanaik, A.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.661-666
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    • 2005
  • The effects of pre-partum feeding management in terms of birth weight, growth, metabolic profile and immunity of calves were studied using 24 crossbred (Bos taurus${\times}$Bos indicus) cows, divided into three equal groups. The dietary treatments included feeding of either 3.0 kg concentrate/head/d throughout the 60 d pre-partum (T$_1$), or 3.0 kg concentrate during 60-22 d pre-partum and thereafter at an increased allowance at 0.25 kg/d during the next 21 d till it reached 1% of live weight (T$_2$). The third group of cows was fed similar to T$_2$, except that the concentrate feeding during 60-22 d pre-partum was reduced to 2.0 kg (T$_3$). All the groups had access to ad libitum green fodder throughout. The results revealed that the mean daily dry matter (DM) intake by the cows was similar (p>0.05) among the three groups during the 60 days of the pre-partum but T2 animals tended to gain more live weight (41.25 kg) than T$_1$ (38.12 kg) and T$_3$ (36.25 kg). The body condition score of the cows did not change appreciably over the experimental period. The mean birth weight of the calves was 24.00${\pm}$1.10, 24.63${\pm}$1.17 and 23.25${\pm}$1.19 kg for the three groups, respectively, with the corresponding average daily gain of 154.2, 155.0 and 169.7 g during the subsequent 60 days; both these parameters did not vary significantly ascribable to prepartum feeding regimens of their dams. The total immunoglobulin (Ig) concentration in the colostrum was 6.31${\pm}$0.34, 5.80${\pm}$0.21 and 6.13${\pm}$0.30 g/dl for the three groups, respectively, showing no influence of dietary treatments. The mean serum Ig levels (T$_1$ 2.10${\pm}$0.09, T$_2$ 2.05${\pm}$0.09 and T$_3$ 2.10${\pm}$0.12 g/dl) of calves at 5 d of age were similar among the dietary groups as was the case with various serum biochemical constituents. It is concluded that the variations in pre-partum dietary management elicited no significant influence on the calf performance including the immune status.