• Bioinformatics and Biosystems
• /
• v.2 no.1
• /
• pp.17-23
• /
• 2007

Shot interfering RNA (siRNA) can be used to silence specific gene expression and have many potential therapeutic applications. However, how to design an effective siRNA is still not clear. Highly effective siRNA has sequence-specific properties which are low G/C content, low internal stability at the sense strand 3'-terminus, sense strand base bias(position 1 is G/C, position 19 is /AU). Recently, mRNA secondary structure playsan important role in RNAi. Target site of siRNA in high-ordered structure (i.e hairpin loop, multi loop) or base pair of many hydrogen bonds dramatically reduce function of siRNA mediated gene silencing. Possible off-target effects of siRNA is detecting from BLAST search.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.8 no.3
• /
• pp.179-192
• /
• 2008

A multiphase compensation method with mismatch linearization technique, is presented and demonstrated in a $\Sigma-\Delta$ fractional-N frequency synthesizer. An on-chip delay-locked loop (DLL) and a proposed delay line structure are constructed to provide multiphase compensation on $\Sigma-\Delta$ quantizetion noise. In the delay line structure, dynamic element matching (DEM) techniques are employed for mismatch linearization. The proposed $\Sigma-\Delta$ fractional-N frequency synthesizer is fabricated in a $0.18-{\mu}m$ CMOS technology with 2.14-GHz output frequency and 4-Hz resolution. The die size is 0.92 mm$\times$1.15 mm, and it consumes 27.2 mW. In-band phase noise of -82 dBc/Hz at 10 kHz offset and out-of-band phase noise of -103 dBc/Hz at 1 MHz offset are measured with a loop bandwidth of 200 kHz. The settling time is shorter than $25{\mu}s$.

• ALGAE
• /
• v.18 no.3
• /
• pp.183-190
• /
• 2003

Except for a group I intron in trnL-uaa occuring in eubacteria and plastids, group I introns are rarely documented in plastid genomes. Here, we report that a green alga, Caulerpa sertularioides, contains three group IA3 introns in the 16S gene (cpSSU), CS-cpSSU.i1, CS-cpSSU.i2 and CS-cpSSU.i3. Each intron has an open reading frame with LAGLIDADG motifs. CS-cpSSU.i1orf and CS-cpSSU.i3orf occur at Loop 6 in the intron secondary structure and CScpSSU. i2orf at Loop 8. CS-cpSSU.i1orf and CS-cpSSU.i2orf contain both LAGLI-DADG motifs but CS-cpSSU.i3orf has only one. CS-cpSSU.i1 and CS-cpSSU.i2 share the insetion sites and the ORFs at Loop 6 and 8 with CpSSU·1 and CpSSU·2 introns of Chlamydomonas pallidostigmatica (Chlorophyceae). In contrast, CS-cpSSU.i3, containing 28 copies of GAAATAT at Loop 6, is a novel intron found only in Caulerpa sertularioides. Possible scenarios of the evolution of the three introns and their possible use in systematic research are discussed.

• Journal of information and communication convergence engineering
• /
• v.10 no.2
• /
• pp.103-107
• /
• 2012

An end-launched adapter combining a coaxial loop within a rectangular waveguide has been analyzed and designed. The accurate electromagnetic modeling of input impedance for the adapter design has been developed using a modal analysis method. Also, design parameters to improve the end-launched adapter have been investigated. Numerical and experimental results of the proposed model have been compared with the results of previous works, and verified by an electromagnetic simulator of High Frequency Structure Simulator (HFSS). The input voltage standing wave ratios (VSWRs) are smaller than 2 over the wide frequency band from 7.5 GHz to 10.5 GHz.

• The Transactions of the Korean Institute of Electrical Engineers P
• /
• v.61 no.1
• /
• pp.5-9
• /
• 2012

In this paper, multi folded dual monopole antenna for WLAN communication of dual bend is designed and fabricated. The proposed multi folded dual monopole antenna are consisted of two folded rectangle loops by microstrip fed that is modified dual monopole antenna. Therefore, the outside rectangle loop structure of the proposed antenna is extended a dual monopole. The characteristics of the proposed antenna is analyzed return loss and radiation patterns by the FDTD tools. As a result a bandwidth of proposed antenna has about 0.82GHz from 2.0 to 2.82[GHz] and 0.7GHz from 5.46 to 6.16[GHz]. It is used WLAN communications of 2[GHz] and 5[GHz].

• Journal of the Korean Chemical Society
• /
• v.42 no.2
• /
• pp.209-213
• /
• 1998

The higher order structure of Pseudomonas alcaligenes 5S rRNA has been investigated by using ($\eta^{6}$-mesitylene) manganese (Ⅰ) tricarbonyl hexafluorophosphate[MTH-Mn (Ⅰ)], dimethylpyrocarbonate, potassium permanganate as chemical probes. The sequences cleaved strongly by MTH-Mn (Ⅰ) on the tertiary structure of the 5S rRNA are $G_{12}AUGG_{16}$ of loop a, $G_{51}AAGUGAAGC_{60}$ of the region b-C, $U_{65}-AGCG_{69}$. of the region B-a, and $G_{72}AUGG_{76}$ of loop d. Based on such cleavage patterns of 5S rRNA by MTH-Mn(Ⅰ) and other chemical probes, we presume that the sequences strongly cleaved form pocket-like structure as in the the corner of L structure of $tRNA^{Phe}$. We also presume that the region b-C and loop d together play a role of hinge in forming the pocket-like structure in the 5S rRNA.

• Journal of Internet Computing and Services
• /
• v.2 no.4
• /
• pp.91-99
• /
• 2001

Since roost of the program execution time is spent in the loop structure, the problem of extracting parallelism from sequential loop has been one of the most important research issues. However. roost programs have Implicit interprocedure parallelism. This paper presents a generalized method extracting parallelism in loops having the procedure calls. Most parallelization of loops having procedure calls focus on the uniform code where data dependency distance is constant. We present algorithms which can be applied to uniform code, nonuniform code, and complex code. The performance of the proposed algorithm, loop extraction, loop embedding and procedure cloning transformation methods have been evaluated using CRAY-T3E. The result shows the effective of the proposed algorithm.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.21 no.2
• /
• pp.339-344
• /
• 2017

A novel structure of phase locked loop (PLL) which has small size and fast locking time with Early-late detector, Duty-rate modulator, and Lock status indicator (LSI) is proposed in this paper. The area of loop filter usually occupying the larger portion of the chip is minimized using a single small capacitor. While the conventional PLL with a single capacitor loop filter cannot work stably, the proposed PLL with two charge pumps works stably because the output voltage waveform of the proposed a single capacitor loop filter is the same as the output voltage waveform of the conventional 2nd-order loop filter. The two charge pumps are controlled by the Early-late detector which detects early-late status of UP and DN signals, and Duty-rate modulator which generates a steady duty-rate signal. Fast locking time is achieved using LSI. It has been simulated and proved by HSPICE in a CMOS $0.18{\mu}m$ 1.8V process.

• Bulletin of the Korean Chemical Society
• /
• v.26 no.12
• /
• pp.2033-2037
• /
• 2005

In vitro selection was used to isolate $Mg^{2+}$-dependent self-cleaving ribozymes with cis-cleavage activity from a pre-tRNA library having 40-mer random sequences attached to 5'-end of E. coli $tRNA^{Phe}$. After 8 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment), RNA molecules which can self-cleave at the high concentration of $Mg^{2+}$ were isolated. The selected ribozymes can carry out the self-cleavage reaction in the presence of 100 mM $Mg^{2+}$ but not in 10 mM $Mg^{2+}$. The cleavage sites of the ribozymes are located at +3 and +4 of $tRNA^{Phe}$, compared with +1 position of 5'-end cleavage site of pre-tRNA by RNase P. New RNA constructs deprived of its D stem-loop, anticodon stem-loop, variable loop and T stem-loop, respectively showed the cleavage specificity identical to a ribozyme having the intact tRNA structure. Also, the new ribozyme fused with both a ribozyme and $tRNA^{Leu}$ showed the cleavage activities at the various sites within its sequences, different from two sites of position +3 and +4 observed in the ribozyme with $tRNA^{Phe}$. Our results suggest that the selected ribozyme is not structural-specific for tRNA.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.18 no.12
• /
• pp.2816-2822
• /
• 2014

In this paper, a wideband loop antenna for UWB applications is studied. The structure of the proposed wideband loop antenna is a circular loop antenna with appended circular sectors to obtain an ultra-wideband characteristic. The circular sectors are used instead of conventional triangular sectors to match with the 50 ohm feed line. Optimal design parameters are obtained by analyzing the effects of the gap between the circular sectors and the radius of the circular loop on the input reflection coefficient and gain characteristics. The optimized wideband loop antenna is fabricated on an FR4 substrate with a dimension of 41 mm by 41 mm. Experiment results show that the proposed antenna has a frequency band of 3.1-11.0 GHz for a VSWR < 2.25, which assures the operation in the UWB band. Measured gain ranges 1.3-5.3 dBi in the UWB band.