Electrophoretic analysis was performed to determine the interspecific genetic relationships of the genus Raja collected in Korea waters. We confirmed 5 species [Raja (Okamejei) meerdervoortii Bleeker, R.(O).acutispina Ishiyama, R.(O) kenojei Muller et Henle, Raja (Dipturus) kwangtungensis Chu, and R.(D.) pulchra Liu] inhabting in Korea. As results of starch gel electrophoresis, 24 genetic loci were obtained from 13 enzymic and non-enzymic proteins, and allele frequencies of each locus were calculated . Even thought their various dorsal color patterns, the mean genetic similarity among 4 conspecific populations of R.(O) kenojei showed highly close genetic relationships (S=0.966) R.(O) kenojei and R.(D) kwangtungensis which belong to different subgenus , revealed subspecific level of differentiation (S=0.829). R. (Diptuuus ) kwangtungensis was genetically much closer to Raja(Okamejei) species than to Raja (Dipturus) species group. R.(O) acutispina and R.(D) kwangtungensis were unrecorded species to Korea.
Proceedings of the Mineralogical Society of Korea Conference
/
2002.05a
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pp.1-3
/
2002
The eastward extension of the suture zone between the Sino-Korea and Yangtze cratons in the Korean Peninsula and Japanese islands remains debatable (Hiroi, 1981; Cluzel et al., 1991; Yin and Nie, 1993; Sohma and Kunugiza, 1993; Isozaki, 1997; Arakawa et at., 2000), and is related to our understanding of the continent-continent collision orogeny. The collision orogeny varies in tectono-metamorphic processes and the timing differs from place to place, as exemplified by the absence of coesite and micro-diamond in the Korean Peninsula and Japanese islands, because it is a long-lived process of more than several tens of million years from subduction to exhumation in the Wilson cycle, and because the suture zone extends more than several thousand kilometers with a curved shape from the Qinling area of China to the Hida highland area of Japan. Hiroi (1981) is the first paper to correlate the Unazuki metamorphic rocks of the Hida metamorphic belt in Japan with the Ogcheon belt in the Korean Peninsula based on the presence of 240 Ma medium P/T metamorphic rocks in both belts, but there is a lack of recent studies on this correlation. To resolve the correlationship, there are two approaches: 1) petrological studies characterizing the origin and P-T history of rocks and 2) in-situ micro-dating of fine-grained, zoned minerals of zircon, monazite, uraninite and thorite using the EPMA (U-Th-Pb chemical dating or CHIME depending on calibration method) and the SHRIMP (Sensitive High-resolution ion Microprobe) to decipher the timing of geological events. As a first step of these approaches, micro-dating was undertaken to rocks of the Hida metamorphic belt and its Mesozoic cover (Tetori Group) in the Hida highland area, central Japan.
In the present study, overexpression, purification, and characterization of Aeropyrum pernix K1 chaperonin B in E. coli were investigated. The chaperonin $\beta$-subunit gene (ApCpnB, 1,665 bp ORF) from the hyperthermophilic archaeon A. pernix K1 was amplified by PCR and subcloned into vector pET21a. The constructed pET21a-ApCpnB (6.9 kb) was transformed into E. coli BL21 Codonplus (DE3). The transformant cell successfully expressed ApCpnB, and the expression of ApCpnB (61.2 kDa) was identified through analysis of the fractions by SDS-PAGE (14% gel). The recombinant ApCpnB was purified to higher than 94% by using heat-shock treatment at $90^{\circ}C$ for 20 min and fast protein liquid chromatography on a HiTrap Q column step. The purified ApCpnB showed ATPase activity and its activity was dependent on temperature. In the presence of ATP, ApCpnB effectively protected citrate synthase (CS) and alcohol dehydrogenase (ADH) from thermal aggregation and inactivation at $43^{\circ}$ and $50^{\circ}$, respectively. Specifically, the activity of malate dehydrogenase (MDH) at $85^{\circ}$ was greatly stabilized by the addition of ApCpnB and ATP. Coexpression of pro-carboxypeptidase B (pro-CPB) and ApCpnB in E. coli BL21 Codonplus (DE3) had a marked effect on the yield of pro-CPB as a soluble and active form, speculating that ApCpnB facilitates the correct folding of pro-CPB. These results suggest that ApCpnB has both foldase and holdase activities and can be used as a powerful molecular machinery for the production of recombinant proteins as soluble and active forms in E. coli.
1) An experiment was made at the newly constructed glass house of Chun chan Agriculture callege to find out facts about the late-raising of Cortinellus edodes (Berk) Ido et Imai during the winter season. 2) The host timbers used in this experiment were Quercus Serrata Thumb and Quercus crispulo BI.; 5 years old each, the diameter ranging from 6cm to 17cm, dividing them into 12 diameter groups with 10 timbers in each group. The total number of timbess was 240 in two blocks. 3) As the pre-treatment of host timber, the autumn generation in 1962 was inhibited, and before being put into the glass-house, they were stimulated of generation for 3 days after 3 days, water-soaking. 4) The temperature control was made by using two coal stoves and the humidity control by spraying and two full water tanks. During cloudy weather and night, the green house was covered to preserue constant temperature of $5^{\circ}C{\sim}10^{\circ}C$. 5) The result; Quercus Serrata Thumb. with lesser diameter than 9cm showed no generation either in as big timber as one with diameter 11 cm. On the other hand, too big timbers showed low rate of generation; the reason, at a guess, is the too short period of cultivation and insufficient water soaking and inappropriate temperature. 6) The most standard size of host timber was found out be those with diameter 12cm~15cm in Quercus serrata Thumb, and those with diameter 14cm~17cm in Quercus crispula BI. Quercus crispula seemed to have slightly greater efficiency of generation than Quercus Serrata Thumb., but it could not be concluded as "decisive". Lastly, the host timber used in this experiment were old and the rate of generation was low as a whole.
Purpose: This study was performed to understand health perception, health practice, and depression of the elderly and to identify their depression level according to health perception and health practice. Method: The subjects were 463 elders who have lived in the Jeju Island and data were collected from June to August in 2005. Data were analyzed by SPSS 10.0. Result: The mean score of depression was 12.94 out of 30, and had a statistically significant correlation with educational level (F=2.943 p=.033), occupation (F=4.611. p=.010), and the number of chronic diseases (F=5.303. p=.001). When the cutoff of 18 points was applied based on Jung et al. (1997), the depression ratio was 12.4%, and when the cutoff parameter by Yesavage et al (1993) was applied, themoderate depression ratio was 74.8% and the severe depression ratio was 3.4%. In health recognition, consideration of usual health state had a significant correlation with depression (F=3.553 p=.007) but consideration of health state compared to the previous year wasn't. In health practice, sleeping was in a significant correlation with depression (F=3.574 p=.011), but smoking, alcohol drinking, exercise, and rest were not. Conclusion: Based on above results, we need further study on another sample group and the development, application, and verification of health management, education, and counseling programs for the elderly. Also, additional research should be made on significant factors of elderly depression.
To investigate antioxidative effects of phenolic compounds separated from persimmon leaves(PL)(Diospyros kaki Thunb.) on the ethanol-induced hepatotoxicity in rat, Sprague-Dawley rats weighing 100-150 g were divided into 5 groups; control group(CON), PL(70 mg/kg) administered group(PEl), ethanol(5 mL/kg, 25%) administered group(ETH), PL(70 mg/kg) and ethanol administered group (PE2), and PL(140 mg/kg) and ethanol administered group(PE3), respectively. The antioxidative activity of persimmon leaves decreased in order of ethylacetate>interphase materials>n-butanol>chloroform>n-hexane>water fraction. The growth rate and feed efficiency ratio decreased by ethanol were gradually increased to the adjacent level of CON by administering PL. The serum activities of ALT, alkaline phosphatase and lactic acid dehydrogenase elevated by ethanol were decreased significantly. It was also observed that the activities of SOD, catalase, and GSH-Px of rat liver increased by ethanol were markedly decreased in PL administered group as compared to ETH. The GSH content of liver was decreased by ethanol, but that was increased in PE1 and PE2 compared with ETH as a dose-dependant manner. These results suggested that phenolic compounds separated from persimmon leaves have a possible protective and relievable effect on the ethanol-induced hepatotoxicity in rats.
Objective: To elucidate 1) whether there are any differences in the urine concentrations of steroid hormone metabolites between patients with leiomyoma and normal controls 2) the correlation between urinary profiles of steroid hormones and leiomyomas of the uterus according to their type, location, volume, and weight. Materials of Methods : The study population consisted of 37 premenopausal patients with uterine leiomyoma and the control group consisted of 25 premenopausal normal volunteer women without uterine leiomyoma. Confirmation of the existence of uterine leiomyoma was done by ultrasonography and histopathological examination after surgery. The volume of the leiomyoma was estimated by trans-abdominal and/or trans-vaginal ultrasonography. The Leiomyomas were divided into 3 types (subserosal, intramural and submucosal). Seventeen patients had subserosal type of leiomyoma, 10 with the intramural type and 10 with the submucosal type. The locations of the leiomyoma were also divided into 3 groups (fundus, body and isthmus). Seventeen patients showed a fundus location, 10 in body, and 10 in isthmus. We compared urinary profiles of the endogenous steroids between patients with leiomyomas and normal controls, and also investigated the relationship between urinary profiles of the endogenous steroids and leiomyomas according to their type, location, volume and weight by using highly sensitive Gas Chromatography-Mass Spectrometry (GC-MS) system. Results: The mean ages of the patients with leiomyomas and the control group were $43.1{\pm}5.6$ and $40.6{\pm}7.2$ years, the weights were $63.4{\pm}7.3$ and $59.4{\pm}8.1\;kg$, and their heights were $155.4{\pm}4.8$ and $159.3{\pm}4.8\;cm$ respectively. Seventeen patients had subserosal, 10 had intramural, and 10 had submucosal leiomyomas. There were 17 patients with leiomyoma located in fundus, 10 in body and 10 in isthmus. $17{\beta}$-estradiol, 5-AT, 11-keto ET, $11{\beta}$-hydroxy An, $11{\beta}$-hydroxy Et, THS, THA, THE, a-cortolone, a-cortol, $\beta$-cortol, $11{\beta}$-OH Et/$11{\beta}$-OH An and E2/E1 were significantly increased in patients with leiomyoma than in the control group. $17{\beta}$-estradiol was significantly increased in the intramural and the submucosal types than in the subserosal type. There was no significant difference in the concentrations of urinary steroids according to the locations of leiomyomas. There was no significant relationship between the concentration of urinary steroids and the volume of the leiomyomas. $17{\beta}$-estradiol significantly decreased as the weight of uterus increased (r=-0.322, p=0.04). Conclusion: The concentrations of steroid hormone metabolites were generally increased in patients with leiomyoma but were not significantly related to the volume and weight of the leiomyomas. Our study suggests that steroid hormones may be involved in the initiation of leiomyomas but may not be involved in their progression. In addition, the concentrations of steroid hormone metabolites are not related to the leiomyoma type and location.
Kim, Mi-Ryang;Lee, Chang Won;Dunham, Michael M.;Evans, Neal J II;Kim, Gwanjeong;Allen, Lori E
The Bulletin of The Korean Astronomical Society
/
v.41
no.2
/
pp.38.3-39
/
2016
We present the results of a search for Very Low-Luminosity Objects (VeLLOs) in the Gould Belt (GB) clouds using infrared and sub-millimeter (sub-mm) data from 1.25 to $850{\mu}m$ and our N2H+ (J = 1-0) observations. We modified the criteria by Dunham et al. to select the VeLLOs in the GB clouds, finding 95 VeLLO candidates, 79 of which are newly identified in this study. Out of 95 sources, 44 were detected in both sub-mm continuum and N2H+ emission and were classified as Group A (the VeLLOs), and 51 sources detected in either sub-mm emission or N2H+ emission were classified with Group B as candidate VeLLOs. We find that these VeLLOs and the candidates are forming in environments different from those of the likely VeLLOs. Seventy-eight sources are embedded within their molecular clouds, and thus are likely VeLLOs forming in a dense environment. The remaining 17 sources are located in low-level extinction regions (Av < 1) connected to the clouds, and can be either background sources or candidate substellar objects forming in an isolated mode. The VeLLOs and the candidates are likely more luminous and their envelopes tend to be more massive in denser environments. The VeLLOs and the candidates are more populous in the clouds where more YSOs form, indicating that they form in a manner similar to that of normal YSOs. The bolometric luminosities and temperatures of the VeLLOs are compared to predictions of episodic accretion models, showing that the low luminosities for most VeLLOs can be well explained by their status in the quiescent phases of a cycle of episodic mass accretion.
Journal of Physiology & Pathology in Korean Medicine
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v.25
no.1
/
pp.71-77
/
2011
Gastric ulcer has multifactorial etiology, and the development of ulcer is known to be caused by gastric acidity, pepsin secretion, gastric motility and gastric mucosal blood flow. The ulcer results from the tissue necrosis and apoptotic cell death triggered by mucosal ischemia, free radical formation and cessation of nutrient delivery. The gastric mucosa is usually exposed to a wide range of aggressive insults, and has developed efficient mechanisms to repair tissue injury. The apoptotic process of gastric mucosa is triggered by the induction of such proapoptotic gene expression, such as BAX. The Bcl-2 family of proteins plays a pivotal role in the regulation of apoptosis. The maintenance of gastric mucosa integrity depends upon the ratio between cell proliferation and cell death. Stress-inducing factors may affect Bcl-2/BAX ratio and thus the rate of apoptosis through modulation of the expression of both proteins depends upon the experimental model. In addition to the regulation of apoptosis, new vessels have to be generated in order to ensure an adequate supply of oxygen and nutrients to the healing gastric mucosa. This events are regulated by several factors. Among them, such polypeptide growth factors, such as vascular endothelial growth factor (VEGF) regulates essential cell functions involved in tissue healing including cell proliferation and differentiation. The purpose of this study was carried to investigate whether Rhei Rhizoma administration might protect apoptotic cell death and promote angiogenesis in gastric mucosa. Sprague-Dawley rats were randomly divided into 4 groups; normal, saline, cimetidine and Rhei Rhizoma-treated group. The saline, cimetidine and Rhei Rhizoma extracts were orally administrated to each group and gastric ulcer was induced by HCl-EtOH solution. After 1 hour, the stomachs were collected for histological observation and immunohistochemistry. In results, Rhei Rhizoma proves to promote to heal wound in gastric ulcer in conclusion and the significant changes of BAX, Bcl-2 and VEGF quantity in gastric mucosa were observed. These results suggest that Rhei Rhizoma extract may promote incision wound healing and has protective effects on gastric ulcer in rats.
Kim, Joong-Hark;Hong, Sung-Kil;Hwang, Sung-Jue;Son, Sang-Wook;Choi, Yong-Soon
Korean Journal of Medicinal Crop Science
/
v.20
no.2
/
pp.108-116
/
2012
In this study, to develop hair growth agents using natural products which has excellent ability to promote hair growth effect and fewer side effect, animal experiment and clinical trials was performed to evaluate hair growth promotion effect of herbal product containing $Rosa$$mutiflora$ roots extracts as a main component (RMHP). 4-week-old male C57BL/6 mice were removed the dorsal hair using thioglycolate, and applied 15% EtOH solution as a negative control, 5% minoxidil as a positive control and RMHP to dorsal skin. In the results of macroscopy and photo-interpretation, RMHP group recorded 100% (+++++) of hair growth was proved to significantly stimulate hair growth against 80% (++++) negative control group. 37 patients were treated with RMHP and evaluated the therapeutic effect at 16 weeks. Hair density was significantly increased at 16 weeks after applying RMHP ($125.0{\pm}4.9\;FU/cm^2$) compared to before treatment ($104.3{\pm}4.7\;FU/cm^2$, p < 0.05), and hair thickness were also significantly increased ($0.066{\pm}0.003$ mm) than before treatment ($0.055{\pm}0.002$ mm, p < 0.05). The result of clinical photo-interpretation using 7-point rating scale assessment, after 16 weeks clinical symptoms were evaluated to significantly improve with $1.23{\pm}0.05$ (p < 0.05). Therefore, the results of this study were observed that RMHP have hair loss prevention effect and hair growth promotion effect to hair loss patients.
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