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SUPEROXIDE DISMUTASE - AND CATALASE - ACTIVITY IN BLOOD PLASMA AND RED BLOOD CELLS IN PERIODONTITIS (치주염 환자의 혈장과 적혈구내 S.O.D와 Catalase 활성도에 관한 연구)

  • Hwang, Seung-Hwan;Kim, Byung-Ok;Han, Kyung-Yoon
    • Journal of Periodontal and Implant Science
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    • v.25 no.1
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    • pp.167-178
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    • 1995
  • It has been believed that the increased release of free oxygen radicals and their tissue damaging potency might be a contributing factor in the pathogenesis of periodontal disease. Antioxidant enzymes such as superoxide dismutase(SOD) and catalase can protect the tissue damage from the free oxygen radicals($O_2^-,H_2O_2$, and $OH^-$). In order to investigate the SOD- and catalase - activity in the blood plasma and red blood cells(RBCs) of the patients with perodontitis, 19 male periodontitis patients($25{\sim}35$ years old) who had good general health, more than 10 teeth with severely inflamed gingiva, attachment loss more than 6mm and bone loss were selected as periodontitis group, and 13 male volunteers($22{\sim}29$ years old) with good general and periodontal health were selected as normal group. After blood plasma and RBC were separated from peripheral blood of 2ml collected from antecubital vein of each subject, SOD- activity in blood plasma and RBCs was measured by the same method that Paoletti et al. did, and catalase - activity in RBC was measured by the same method that Beers et al, did. The difference of SOD- and catalase - activity between the normal and the periodontitis groups was statistically analyzed by Student t-test with SPSS/PC program.The results were as follows : 1. SOD activity in blood plasma was significantly lower in the periodontitis group($1.986{\pm}0.893$) than in the normal group($3.324{\pm}1.044$)(p<0.05). 2. There was no statistical significance in the difference of SOD- activity in RBCs between the periodontitis group($7.753{\pm}3.206$) and the normal group($8.116{\pm}1.192$)(p$242.8{\pm}45.6$) than in the normal group($280.2{\pm}32.6$)(p

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Fifteen Newly Recorded Species of the Subfamily Doryctinae (Hymenoptera) in Korea

  • Lee, Hye-Rin;Belokobylskij, S.A.;Ku, Deok-Seo;Byun, Bong-Kyu
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.1
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    • pp.17-24
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    • 2020
  • Doryctinae is a large and heterogeneous group with more than 1,000 described. It is idiobiont ectoparasitoids on the larvae of wood-boring and xylophagous beetles. Some species attack larvae of wood boring lepidoptera. In the present study, fifteen species belonging to eight genera of the subfamily Doryctinae are recorded for the first time from Korea: Doryctes Haliday (2 species), Eodendrus Belokobylskij (1 species), Heterospilus Haliday (4 species), Monolexis Förster (1 species), Neurocrassus Snoflak (2 species), Rhoptrocentrus Marshall (1 species), Sonanus Belokobylskij et Konishi (1 species), Spathius Nees (3 speices). The genera Eodendrus Belokobylskij, Monolexis Förster, Rhoptrocentrus Marshall, Sonanus Belokobylskij et Konishi and fifteen species are reported for the first times from Korea. Diagnosis and host information are provided.

The Taxonomy of Dirty Data for MPEG-2 TS (MPEG-2 표준을 위한 오류 데이터 분류)

  • 곽태희;최병주
    • Proceedings of the Korean Information Science Society Conference
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    • 2001.04a
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    • pp.691-693
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    • 2001
  • DASE(Digital TV Application Software Environment)는 데이터 방송을 위한 국제 표준으로 MPEG-2 TS(Moving Picture Experts Group-2 Transport Stream) 형식의 데이터를 처리한다. 소스코드 대신 입력 데이터 명세 정보만을 공개하는 특성상 DASE 시스템의 오류를 테스트하기 위해서는 테스트 데이터에 오류를 삽입하는 방법이 적합하고 이를 위해 MPEG-2 표준을 위한 오류 항목을 개발이 요구된다. 본 논문에서는 관계형 데이터 베이스를 위한 데이터 분류인 Kim’s et al 분류를 근거로 하여 MPEG-2 표준을 위한 오류 항목을 개발하였다. 이는 DASE 시스템의 오류 삽입 테스트 기법에 유용하게 사용될 수 있을 것이다.

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Study on pharmacological activities of the roots of Cicorium intibus

  • Yim, Dong-sool;Kim, Kwang-sub;Cheong, Jae-hoon;Lee, Sook-yeun
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1997.04a
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    • pp.74-74
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    • 1997
  • Chicory is used popularly. We use leaves of the plant as ordinary mea1, and roots as a substitute of tea materials. It also has been asserted that it has clinical effects on weakness, hepatic disease, diabetes, etc. However, experimental evidences are so insufficient that we started these studies. For antiinflammatory activity, MeOH Ex. was orally administered to rats, and decreased amounts of paw edema induced by carrageenan injection were measured. For bile secretion increament, rats were administered total MeOH, EtOAc fraction, and BuOH fraction Ex. respectively. One hour later, bile ducts were cannulated, and we collected bile every 20 minutes for 4 hours. For hepatoprotective activity, CCl$_4$-intoxicated mouse were treated with MeOH Ex., then s-GPT, S-GOT, and liver weight were measured. For antidiabetic activity, rats were induced diabetes by streptozocin 45mg/kg(i.v) injection. One week later, 1000mg/kg of total MeOH Ex. of chicory root was orally administered. We divided rats into three groups. Group 1 rats were administered only once, group 2 ones once a day for one week, and group 3 ones for three weeks. The concentrations of serum glucose were measured before and after administration. For antihypertensive activity, SHR were administrated total MeOH Ex. of chicory once a day for 8 days, and were measured blood pressure on 1st, 3rd, 6th and 8th day. Total MeOH, EtOAc fraction, and BuOH fraction Ex. increased bile secretion in rats, and decreased liver toxicity induced by CCl$_4$ in mouse. Total MeOH, Ex. of chicory roots has antiinflammatory effect, and decreased blood glucose concentration in group 2 and 3 rats. It was revealed not lowering blood pressure significantly in SHR.

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A Formula for Calculating Dst Injection Rate from Solar Wind Parameters

  • Marubashi, K.;Kim, K.H.;Cho, K.S.;Rho, S.L.;Park, Y.D.
    • Bulletin of the Korean Space Science Society
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    • 2009.10a
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    • pp.36.3-37
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    • 2009
  • This is an attempt to improve a formula to predict variations of geomagnetic storm indices (Dst) from solar wind parameters. A formula which is most widely accepted was given by Burton et al. (1975) over 30 years ago. Their formula is: dDst*/dt = Q(t) - Dst*(t)/$\tau$, where Q(t) is the Dst injection rate given by the convolution of dawn-to-dusk electric field generated by southward solar wind magnetic field and some response function. However, they did not clearly specify the response function. As a result, misunderstanding seems to be prevailing that the injection rate is proportional to the dawn-to-dusk electric field. In this study we tried to determine the response function by examining 12 intense geomagnetic storms with minimum Dst < -200 nT for which solar wind data are available. The method is as follows. First we assume the form of response function that is specified by several time constants, so that we can calculate the injection rate Q1(t) from the solar wind data. On the other hand, Burton et al. expression provide the observed injection rate Q2(t) = dDst*/dt + Dst*(t)/$\tau$. Thus, it is possible to determine the time constants of response function by a least-squares method to minimize the difference between Q1(t) and Q2(t). We have found this simple method successful enough to reproduce the observed Dst variations from the corresponding solar wind data. The present result provides a scheme to predict the development of Dst 30 minutes to 1 hour in advance by using the real time solar wind data from the ACE spacecraft.

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Domain Expression of ErmSF, MLS (macrolide-lincosamide-streptogramin B) Antibiotic Resistance Factor Protein (MLS (macrolide-lincosamide-streptogramin B) 항생제 내성인자 단백질인 ErmSF의 domain발현)

  • 진형종
    • Korean Journal of Microbiology
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    • v.37 no.4
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    • pp.245-252
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    • 2001
  • Erm proteins, MLS (macrolide-lincosamide-streptogramin B) resistance factor proteins, show high degree of amino acid sequence homology and comprise of a group of structurally homologous N-methyltransferases. On the basis of the recently determined structures of ErmC` and ErmAM, ErmSF was divided into two domains, N-terminal end catalytic domain and C-terminal end substrate binding domain and attempted to overexpress catalytic domain in E. coli using various pET expression systems. Three DNA fragments were used to express the catalytic domain: DNA fragment 1 encoding Met 1 through Glu 186, DNA fragment 2 encoding Arg 60 to Glu 186 and DNA fragment 3 encoding Arg 60 through Arg 240. Among the pET expression vectors used, pET 19b successfully expressed the DNA fragment 3 and pET23b succeeded in expression of DNA fragment 1 and 2. But the overexpressed catalytic domains existed as inclusion body, a insoluble aggregate. To assist the soluble expression of ErmSF catalytic domains, Coexpression of chaperone GroESL or Thioredoxin and lowering the incubation temperature to $22^{\circ}C$ were attempted, as did in the soluble expression of the whole ErmSF protein. Both strategies did not seem to be helpful. Solubilization with guanidine-HCl and renaturation with gradual removal of denaturant and partial digestion of overexpressed whole ErmSF protein (expressed to the level of 126 mg/ι culture as a soluble protein) with proteinase K, nonspecific proteinase are under way.

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The Effect of Music Experiences on Language Development in Child Care Toddler (만2세 영아의 음악경험이 언어발달에 미치는 영향)

  • Hwang, Inju
    • Korean Journal of Child Studies
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    • v.29 no.2
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    • pp.73-87
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    • 2008
  • This research studied the effects of music experiences on the language development of toddlers in the day care setting. Subjects were 44(23 experimental group; 21 control group) 29- to 42-month-old children. The experimental group experienced 10 weeks of music activities, including musical sense, singing, listening, playing instruments, improvisation, finger plays, movement, simple games, word-sound music games and music routines. Language development was tested by the Preschool Receptive-Expressive Language Scale(Kim et al. 2003). Significant differences were found in the receptive language development of 30- to 36-month-old and in the expressive language development of 36- to 42-month-old toddlers, respectively.

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Effects of Administration of Oxytocin Antagonist on Implantation and Pregnancy Rates in Patients with Repeated Failure of IVF/ICSI Treatment (체외수정시술의 반복적인 실패 환자에서 옥시토신 길항제 주입이 착상 및 임신에 미치는 영향)

  • Ahn, Jun-Woo;Kim, Chung-Hoon;Kim, So-Ra;Jeon, Gyun-Ho;Kim, Sung-Hoon;Chae, Hee-Dong;Kang, Byung-Moon
    • Clinical and Experimental Reproductive Medicine
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    • v.36 no.4
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    • pp.275-281
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    • 2009
  • Objective: This study was performed to evaluate the effect of oxytocin antagonist on the outcome of IVF/ICSI cycles in infertile patients with repeated failure of IVF/ICSI treatment. Method: Forty patients who had experienced two or more failures of IVF/ICSI treatment without low ovarian reserve, were recruited for this prospective randomized study. All patients received controlled ovarian stimulation (COS) using GnRH antagonist multidose protocol (MDP). For the intervention group, intravenous administration of atosiban (mixed vasopressin $V_{1A}$/oxytocin antagonist) started with a bolus dose 6.75 mg one hour before embryo transfer (ET) and continued at an infusion rate of 18 mg/hour. After ET, administered atosiban was reduced to 6 mg/hour and continued for 2 hours. The main efficacy endpoints were clinical pregnancy rate and implantation rate. Results: Patients' characteristics were comparable in the intervention and control groups. COS parameters and IVF results were also similar. The number of uterine contractions for 3 minutes measured just before ET was significantly lower in the intervention group than control group ($3.5{\pm}1.4$ vs $8.7{\pm}2.2$, p<0.001). While there was no statistically significant difference in the clinical pregnancy rate between control group and intervention group (20.0% and 40.0%, p=0.168), the implantation rate was significantly higher in the intervention group, with 16.9% (11/65) compared with 6.0% (4/67) in the control group (p=0.047). There were no differences in ectopic pregnancy rate and miscarriage rate between the two groups. Conclusion: This study demonstrates that administration of oxytocin antagonist during ET can improve the implantation rate probably by decreasing the frequency of uterine contractions in infertile patients undergoing IVF/ICSI treatment.

ZERO-KNOWLEDGE GROUP IDENTIFICATION AND HIDDEN GROUP SIGNATURE FOR SMART CARDS USING BILINEAR PAIRINGS

  • Lee, Young Whan;Choi, Byung Mun
    • Journal of the Chungcheong Mathematical Society
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    • v.20 no.4
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    • pp.355-366
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    • 2007
  • In this paper, we propose a new blind group identification protocol and a hidden group signature protocol as its application. These protocols involve many provers and one verifier such that (1) the statement of all the provers are proved simultaneously, (2) and also all the provers using computationally limited devices (e.g. smart cards) have no need of computing the bilinear pairings, (3) but only the verifier uses the bilinear pairings. A. Saxena et al. proposed a two-round blind (group) identification protocol in 2005 using the bilinear pairings. But it reveals weakness in the active-intruder attack, and all the provers as well as the verifier must have devices computing bilinear pairings. Comparing their results, our protocol is secure from the active-intruder attack and has more fit for smart cards. In particular, it is secure under only the assumption of the hardness of the Discrete-Logarithm Problem in bilinear groups.

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The Effects of Literature-based Reading Instruction on Children's Literacy (문학작품을 통한 읽기 지도 전략이 초등학교 아동의 문식성에 미치는 효과)

  • Kim, Sun-Deok;Jang, Yeon-Jip
    • Korean Journal of Child Studies
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    • v.21 no.4
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    • pp.243-257
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    • 2000
  • This empirical test of the efficacy of the literature-based reading instruction was conducted with 63(31 male and 32 female) 2nd grade elementary school children. Subjects in the experimental group had 40-45 minutes literature-based reading instruction twice weekly; those in the control group had only basic text reading. Procedures included a pilot study, pre-test, experimental period, and post-test. Research instruments included the Basic Learning Skill Test(Park et al., 1988), the Qualitative Reading Inventory(Leslie & Caldwell, 1990), and the Elementary Reading Attitude Survey(Mckenna & Kear, 1990). Data were graded and scored by each research question and then analyzed with a t-test of differences between the groups. The experimental group showed higher word recognition, text comprehension, and story grammar strategies than the control group. They also showed more improvement in each of these categories than the control group.

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