• The Korean Journal of Ecology
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• 제10권1호
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• pp.23-31
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• 1987

This study was carried out to investigate by the MGP analysis the composition of oribatid mite in coniferous and broad-leaved forests, Kwang Yng experiment plantation, Chola Nam Do Province, southern part of Korea. In these study area, 157 species of oribatid mites (Acari: Cryptogimata) were identified. Among them 6 species such as; Brachychochthonius jugatus JACOT, B hungaricus BALOGH, Eremulus translamealtus BALOGH et. MAHUNKA, Brachioppiella ctenifera GOLOSOVA, Striatoppia opuntiseta BALOGH et. MAHUNKA, Suctobelba perdentata baculifer BALOGH et. MAHUNKA have not been described in Korea. Species compositions were as follows; Among them 69 species were found in all the six sampling sites, 33 species scattered sporadically in all the area, 12 species found in coniferous forest, 10 species in broad-leaved forest only, and 33 species sampled at one site. According to the MGP analysis I, site B-1, B-2, and B-3 were found to be "Type G" and site C-1, C-2, and C-3 were "Type MG". According to the MGP analysis II, site B were found to be all "Type G". But site C-1 was "Type MG", C-2 was "Type M" and C-3 was "Type G" suggesting that "Group M" increased in the site C.e G" suggesting that "Group M" increased in the site C. in the site C.

• 동의생리병리학회지
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• 제26권6호
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• pp.908-914
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• 2012

To investigate the difference and change of ingredient and efficacy of Galgeun-tang (GGT) according to extraction solvent, water and 70% EtOH, the quantities of index components and the several in vitro activities of two kinds of GGT extract were compared. The contents of extracts were analyzed with HPLC. The biological activities such as anti-inflammatory and anti-obesity effects were measured through cell line-based in vitro assay. The cytotoxicity was measured by CCK-8 assay in RAW 264.7 cell, BEAS-2B cell, HaCaT cell and 3T3-L1 cell. We compared effects of two kinds of extract by measuring NO, PGE2, IL-6 and TNF-${\alpha}$ in RAW264.7 cell, RANTES in BEAS-2B cell, MDC and RANTES in HaCaT cell and GPDH activity and leptin level in differentiated 3T3-L1. 70% EtOH extract of GGT contained more compositions than water extract. The inhibitory effect of water extract of GGT on NO in RAW 264.7 cell and GPDH activity in 3T3-L1 was stronger than that of 70% EtOH. 70% EtOH has a stronger inhibitory effect on PGE2 in RAW264.7 cell, RANTES in BEAS-2B cell, MDC, RANTES in HaCaT cell, leptin in 3T3-L1 cell than water extraction. These results suggest that the ingredient and efficacy from 70% EtOH extract of GGT are more effective than water extract.

• 한국식품영양과학회지
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• 제36권3호
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• pp.298-300
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• 2007

머위 에탄올 추출물이 알코올 투여로 유발된 간손상에 미치는 영향을 알아보기 위하여 흰쥐에게 정상식이군(NOR), 알코올 투여군(35% alcohol 10 mL/kg bw, CON), 머위 에탄올 추출물 투여군(PJ1), 알코올과 머위 에탄올 추출물 저용량(200 mg/kg bw) 병합투여군(PJ2) 및 알코올과 머위 에탄올 추출물 고용량(400 mg/kg bw) 병합투여군(PJ2)의 5군으로 나누어 6주간 사육하였다. 체중증가율 및 식이효율, 혈청 중 ALT와 AST 활성, 간손상 억제효과를 확인하기 위하여 항산화효소인 SOD, catalase, XO 및 GSH-Px 활성을 측정하였고, 지질과산화물과 GSH 함량을 측정하였다. 항산화 지표를 측정한 결과 정상식이만을 급여한 NOR군과 정상식이에 머위 에탄올 추출물을 투여한 PJ1군 간에는 유의차가 없었으나, 알코올을 투여한 군(CON, PJ2 및 PJ3)들 간에는 유의차를 보였으며 특히, 고용량 병합투여 시 항산화효과가 높게 나타났다. 머위 에탄올 추출물 병합투여는 만성 알코올 투여로 감소되어진 체중을 정상 체중으로 회복시켰으며, 알코올 투여로 증가된 유리기 해독계 효소인 catalase 및 GSH-Px 활성 억제와 비효소적 항산화작용을 나타내는 GSH 함량을 증가시킴으로써 지질과산화물에 대한 방어력이 증강됨을 보였고, 또한 혈청 중 AST 활성을 유의하게 감소시킴으로써 알코올 섭취로 유도된 지방간 및 손상된 간조직을 보호하는 항산화효과가 있는 것으로 추정된다.

• Clinical and Experimental Reproductive Medicine
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• 제31권3호
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• pp.169-176
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• 2004

Objective: This study was performed to evaluate and compare the embryonic developmental capacity and pregnancy rates in conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) with ejaculated sperm or testicular sperm cycles. Materials and Methods: Fertilization was examined in the following morning after IVF (group I), ICSI (group II) or TESE-ICSI cycles (group III). Fertilized oocytes were co-cultured with Vero cells until embryo transfer (ET). On day 2 and $5{\sim}7$, grades of embryos (<4- or $\geq$4-cell) and blastocysts (BG1, 2, 3 or early) were evaluated. Clinical pregnancy rate was determined by detecting G-sac with transvaginal ultrasonogram. We analyzed the results by $X^2$ and Student's t-test and considered statistically significant when P value was less than 0.05. Results: Fertilization rate was significantly higher (p<0.05) in group I ($79.0{\pm}21.2%$) than in group II and III ($56.8{\pm}21.6%$ and $36.7{\pm}25.3%$). Cleavage and blastulation rate of group I ($95.8{\pm}13.8%$ and $59.5{\pm}25.3%$) were significantly higher (p<0.05) than those of group III ($83.4{\pm}18.6%$ and $40.4{\pm}36.5%$). Clinical pregnancy rate was significantly higher (p<0.05) in group I and II (40.7% and 41.7%) than that in group III (12.5%). No differences were found in the rates of multiple pregnancy and abortion among three groups. Embryonic implantation rate was higher in group I ($15.1{\pm}20.2%$, p<0.05) and II ($14.7{\pm}20.6%$, NS) than that in group III ($5.1{\pm}15.6%$). However, embryonic implantation rate was increased in ET with blastocyst(s) among three groups. Conclusions: Fertilized oocytes obtained from TESE-ICSI were harder to be successfully cultured to blastocyst stage for 5$\sim$7 days than that from IVF cycles. However, all blastocyst(s) ET increased the embryonic implantation rate equally in IVF, ICSI and TESE-ICSI cycles.

• Asian-Australasian Journal of Animal Sciences
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• 제29권6호
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• pp.838-844
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• 2016

This study assessed the influence of genetic type (Embden-Anser anser, EE; Toulouse-Anser anser, TT and F1 cross, ET) for meat characteristics (carcass, meat quality and fatty acid (FA) profiles), of domestic geese "Anser anser domesticus" raised in dehesa as an alternative, organic feeding system. Carcass and breast muscle weight (p<0.01) were greater for the ET group at the same live weight. None of the groups showed differences in the production of fatty liver with this type of feeding. Higher values were found for maximum Warner-Bratzler shear force (between 7.62 and $8.87kg/cm^2$), which implies the improvement of this parameter. High levels of oleic FAs were obtained, especially for the TT group. The polyunsaturated/saturated FA ratio was highest for the ET group (p<0.001), reflecting the optimum nutritional values as a component of a healthy consumer diet.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1154-1154
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• 2001

Near infra-red (NIR) spectroscopy has been used for the non-invasive assessment of intact fruit for eating quality attributes such as total soluble solids (TSS) content. However, little information is available in the literature with respect to the robustness of such calibration models validated against independent populations (however, see Peiris et al. 1998 and Guthrie et al. 1998). Many studies report ‘prediction’ statistics in which the calibration and prediction sets are subsets of the same population (e. g. a three year calibration validated against a set from the same population, Peiris et al. 1998; calibration and validation subsets of the same initial population, Guthrie and Walsh 1997 and McGlone and Kawano 1998). In this study, a calibration was developed across 84 melon fruit (R$^2$= 0.86$^{\circ}$Brix, SECV = 0.38$^{\circ}$Brix), which predicted well on fruit excluded from the calibration set but taken from the same population (n = 24, SEP = 0.38$^{\circ}$Brix with 0.1$^{\circ}$Brix bias), relative to an independent group (same variety and farm but different harvest date) (n = 24, SEP= 0.66$^{\circ}$ Brix with 0.1$^{\circ}$Brix bias). Prediction on a different variety, different growing district and time was worse (n = 24, SEP = 1.2$^{\circ}$Brix with 0.9$^{\circ}$Brix bias). Using an ‘in-line’ unit based on a silicon diode array spectrometer, as described in Walsh et al. (2000), we collected spectra from fruit populations covering different varieties, growing districts and time. The calibration procedure was optimized in terms of spectral window, derivative function and scatter correction. Performance of a calibration across new populations of fruit (different varieties, growing districts and harvest date) is reported. Various calibration sample selection techniques (primarily based on Mahalanobis distances), were trialled to structure the calibration population to improve robustness of prediction on independent sets. Optimization of calibration population structure (using the ISI protocols of neighbourhood and global distances) resulted in the elimination of over 50% of the initial data set. The use of the ISI Local Calibration routine was also investigated.

• 한국응용과학기술학회지
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• 제1권1호
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• pp.33-36
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• 1984

백쥐의 사료(飼料)에 석이버섯의 수용성(水溶性) 및 EtOH 추출물(抽出物)을 지(知)하여 지질대사(脂質代謝) 및 cholesterol대사(代謝)에 미치는 영향을 검토(檢討)한 결과(缺課), 다음과 같은 결론(結論)을 얻었다. (1) 간희(肝曦) 및 혈(血) cholesterol 저하작용(低下作用)에 관해서는 수용성(水溶性) 추출물(抽出物)이나 EtOH 추출물(抽出物)이 동시(同時)에 유핵(有劾)하다는 결과(結果)를 얻었다. 그리고 EtOH 추출물(抽出物)이 수용성(水溶性) 추출물(抽出物)보다 약간 높은 경향을 보였다. (2) 이 cholesterol 저하작용(低下作用)에 관여하는 유핵성출(有劾性物)은 물이나 EtOH의 추출(抽出)에 따라서 어느 쪽의 추출액으로도 이행(移行)되는 성질(性質)을 가지고 있다고 추정(推定)된다.

• Clinical and Experimental Reproductive Medicine
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• 제20권2호
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• pp.117-123
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• 1993

The condition of the endometrium is an important factor which may influence the success or failure in IVF-ET. This study was undertaken for evaluation of the value of endometrial growth as an early predictor for the success of IVF. Ultrasonographic endometrial measurement were performed in 43 IVF cycles that conceived, 101 cycles that did not with an IVF-ET There was no significant difference in the endometrial thickness and the serum concentration of estradiol in the pregnant versus nonpregnant group(10.4 vs. 9.9 mm: 2348 vs. 2017 pg/ml no hCG administration day). No correlation was found between the ultrasound image and serum estradiol levels around the time of hCG administration(r=0.54, p=0.13 no Day 2; r=0.45, p=0.14 no Day 1). The duration of gonadotropin treatment, number of follicles, number of oocytes retrieved, and fertilization rate were not statistically different in the two groups, however, there was a significant difference in the number of embryos in the pregnant versus nonpregnant group)p< 0.05). A higher pregnancy rate and ongoing pregnancy rate occured with an endometrial thickness over 11 mm compared with below 7mm(p< 0.05, p< 0.005). however, no significant differences were noted in the implantation rate and abortion rate among the groups that classified according to their endmetrial thickness. The endometrial growth(${\Delta}$) from hCG administration day(DO) to D6 was greater in the women who achieved pregnancy than in the nonpregnant group(p< 0.01). There were no significant differences in serum estradiol levels, implantation rate, pregnancy rate, and abortion rate among the groups that classified according to the pattern of echogenesity of endometrium, however, significantly higher ongoing pregnancy rate was noted in group A, B compared with group C.(p< 0.0001, p< 0.001) These results suggest that there were no ultrasonographically detectable differences in the patterns of endometrial growth and development around the time of hCG administration in patients who conceive versus those that do not in IVF-ET.

• 대한한의학방제학회지
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• 제26권2호
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• pp.113-122
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• 2018

Objectives : Alcoholic fatty liver is an early and reversible consequence of excessive alcohol consumption. The initial hepatocyte cell death stimulates subsequent inflammatory responses, leading to further liver injury and fibrosis. The objective of this study is to investigate the effects of Injinsaryung-san extract on the alcoholic fatty liver by chronic EtOH administration. Method : Male Sprague Dawley rats were used in this study. All animals were randomly divided into Normal group, treated with saline (n=10); EtOH group, treated with ethanol (n=10); EtOH+IS group, treated with ethanol+Injinsaryung-san extract (n=10). For oral administration of ethanol in Control and Sample group, the ethanol was dissolved in distilled water in concentrations of 25%(v/v). Throughout the experiment of 8 week, the rats were allowed free access to water and standard chow. Sample group were administrated by Injinsaryung-san extract daily for 8 weeks. Results : The levels of hepatic marker such as aspartate aminotransferase and alanine aminotransferase were altered. Histopathological changes were reduced and the expression of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) was markedly attenuated by Injinsaryung-san extract. Conclusion : These data suggest that Injinsaryung-san extract could be effective in protecting the liver from alcoholic fatty liver. The hepatoprotective mechanisms of Injinsaryung-san may be related to attenuation of $TNF-{\alpha}$ protein, as well as to the inhibition of inflammatory response in the liver. Therefore, Injinsaryung-san can be a candidate to protect against alcoholic fatty liver.

• 대한지하수환경학회지
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• 제1권1호
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• pp.6-9
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• 1994

지하수의 Ar/$N_2$가스의 비율은 탈질화(denitrification)의 정도를 측정하는 하나의 방법으로 사용된다. (Barnes et al., 1975; Vogel et al., 1981; Mariotti et al., 1988) 그 비율 측정은 일반적으로 질량분석기 (Mass Spectrometer)를 사용한다. 본 실험에서 사용한 Mass Spectrometer는 일반적인 Static Mass Spectrometer가 아닌 Ar과 $N_2$을 시간적으로 동시에 측정하여 오차를 줄일 수 있는 VG Optima isotope ratio Mass Spectrometer를 사용하였다. 두 지역 지하수의 Ar/$N_2$가스의 비율을 측정한 결과 Site 1(Grand Island, NE)의 경우 대기가스가 포화된 증류수의 Ar/$N_2$가스 비율인 0.026보다 낮은 0.015-0.018로서 탈질이 일어났음을 알 수 있으며, Site 2(Shelton, NE)의 경우 0.023-0.02로서 탈질이 일어나지 않음을 알 수 있다. 본 실험의 목적은 지하수에 응해되어 있는 Ar/Nitrogen의 비율을 정확히 측정하는 방법을 개발하므로서 탈질의 정도를 정확히 측정하며 탈질의 전개 과정을 관찰하기 위한 것이다.