• JSTS:Journal of Semiconductor Technology and Science
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• v.1 no.4
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• pp.239-247
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• 2001

The Fluorescence-Activated Cell Sorter (FACS) is a well-established instrument used for identifying, enumerating, classifying and sorting cells by their physical and optical characteristics. For a miniaturized FACS device, a disposable plastic microchip has been developed which has a hydrodynamic focusing chamber using soft lithography. As the characteristics of the spatially confined sample stream have an effect on sample throughput, detection efficiency, and the accuracy of cell sorting, systematic fluid dynamic studies are required. Flow visualization is conducted with a laser scanning confocal microscopy (LSCM), and three-dimensional flow structure of the focused sample stream is reconstructed from 2D slices acquired at $1\mutextrm{m}$ intervals in depth. It was observed that the flow structure in the focusing chamber is skewed by unsymmetrical velocity profile arising from trapezoidal cross section of the microchannel. For a quantitative analysis of a microscopic flow structure, Confocal Micro-PIV system has been developed to evaluate the accelerated flow field in the focusing chamber. This study proposes a method which defines the depth of the measurement volume using a detection pinhole. The trajectories of red blood cells (RBCs) and their interactions with surrounding flow field in the squeezed sample stream are evaluated to find optimal shape of the focusing chamber and fluid manipulation scheme for stable cell transporting, efficient detection, and sorting

• Smart Structures and Systems
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• v.18 no.6
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• pp.1189-1202
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• 2016

This paper describes an application of wavelet analysis for damage detection of a steel frame structure with bolted connections. The wavelet coefficients of the acceleration response for the healthy and loosened connection structure were calculated at each measurement point. The difference of the wavelet coefficients of the response of the healthy and loosened connection structure is selected as an indicator of the damage. At each node of structure the norm of the difference of the wavelet coefficients matrix is then calculated. The point for which the norm has the higher value is a candidate for location of the damage. The above procedure was experimentally verified on a laboratory-scale 2-meter-long steel frame. The structure consists of 11 steel beams forming a four-bay frame, which is subjected to impact loads using a modal hammer. The accelerations are measured at 20 different locations on the frame, including joints and beam elements. Two states of the structure are considered: healthy and damaged one. The damage is introduced by means of loosening two out of three bolts at one of the frame connections. Calculating the norm of the difference of the wavelet coefficients matrix at each node the higher value was found to be at the same location where the bolts were loosened. The presented experiment showed the effectiveness of the wavelet approach to damage detection of frame structures assembled using bolted connections.

• Publications of The Korean Astronomical Society
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• v.15 no.spc2
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• pp.21-25
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• 2000

The ionosphere, the atmosphere of the earth ionized by solar radiations, has been strongly varied with solar activity. The ionosphere varies with the solar cycle, the seasons, the latitudes and during any given day. Radio wave propagation through or in the ionosphere is affected by ionospheric condition so that one needs to consider its effects on operating communication systems normally. For examples, sporadic E may form at any time. It occurs at altitudes between 90 to 140 km (in the E region), and may be spread over a large area or be confined to a small region. Sometimes the sporadic E layer works as a mirror so that the communication signal does not reach the receiver. And radiation from the Sun during large solar flares causes increased ionization in the D region which results in greater absorption of HF radio waves. This phenomenon is called short wave fade-outs. If the flare is large enough, the whole of the HF spectrum can be rendered unusable for a period of time. Due to events on the Sun, sometimes the Earth's magnetic field becomes disturbed. The geomagnetic field and the ionosphere are linked in complex ways and a disturbance in the geomagnetic field can often cause a disturbance in the F region of the ionosphere. An enhancement will not usually concern the HF communicator, but the depression may cause frequencies normally used for communication to be too high with the result that the wave penetrates the ionosphere. Ionospheric storms can occur throughout the solar cycle and are related to coronal mass ejections (CMEs) and coronal holes on the Sun. Except the above mentioned phenomena, there are a lot of things to affect the radio communication. Nowadays, radio technique for probing the terrestrial ionosphere has a tendency to use satellite system such as GPS. To get more accurate information about the variation of the ionospheric electron density, a TEC measurement system is necessary so RRL will operate the system in the near future.

• Particle and aerosol research
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• v.7 no.3
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• pp.87-97
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• 2011

In this study, we organized the test results obtained from the performance tests for the CA certificated air cleaners which had been commercially available in Korea since 2003, and analyzed the correlation among the test parameters such as flow rate, particle collection efficiency, clean air delivery rate (CADR), ozone emission, odor removal efficiency and noise level etc. The noise level of 267 air cleaners were increased as concentrated at the 45, 50, 55 dB, which are the required noise level for CA certification according to flow rate, and ozone emissions from the CA air cleaners were significantly lower than the requirement limit, 50 ppb for 24 hour operation. The average particle collection efficiency and odor removal efficiency were 89.3 and 80.8%, approximately 20% higher than the requirement of CA certification, regardless of flow rates. The particle removal performance of an air cleaner was clearly discriminated by its CADR, and the CADR was obtained with a simple calculation: 0.79 x flow rate. The low differentiation of gas removal performance of air cleaners by the current CA gas test method was improved by 3.2, 751.3, 13.4 times for ammonia, acetic acid, respectively, by adopting the CADR concept and the real time measurement method, FTIR, for gas removal performance test.

• Environmental Mutagens and Carcinogens
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• v.21 no.2
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• pp.89-94
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• 2001

Recently, single cell gel electrophoresis, also known as comet assay, is widely used for the detection and measurement of DNA strand breaks in vitro and in vivo in many toxicological fields such as radiation exposure, human monitoring and toxicity evaluation. As well defined, comet assay is a sensitive, rapid and visual method for the detection of DNA strand breaks in individual cells. Briefly, a small number of damaged cells suspended in a thin agarose gel on a microscope slide were lysed, unwinded, electrophoresed, and stained with a fluorescent DNA binding dye. The electric current pulled the charged DNA from the nucleus such that relaxed and broken DNA fragments migrated further. The resulting images which were subsequently named for their appearance as comets, were measured to determine the extent of DNA damages. However, some variations could be occurred in procedures, laboratories's conditions and kind of cells used. Hence, to overcome and to harmonize these matters in comet assay, International Workshop on Genotoxicity Test Procedure (IWGTP) was held with several topics including comet assay at Washington D.C. on March, 1999. In spite of some consensus in procedures and conditions in IWGTP, there are some problems still remained to be solved. In this respect, we attempted to set the practical optimal conditions in the experimental procedures such as lysis, unwinding, electrophoresis and neutralization conditions and so on. First of all, we determined optimal lysis and unwinding time by using 150 $\mu$M methyl methanesulfonate (MMS) which is usually used concentration. And then, we determined optimal positive control concentrations of benzo(a)pyrene (BaP) and MMS in the presence and absence of S9 metabolic activation system, respectively.

• KSBB Journal
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• v.4 no.1
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• pp.34-39
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• 1989

Growth kinetics of mammalian cell, Chinese Hamster Ovalry(CHO) was investigated to effectively produce pharmaceutically important Erythropoeitin under perfusion chemostat conditions. Perfusion rate, D is correlated with total viable is to be an essential factor in controlling growth kinetic parameters under this kind of operations. It is also found that the measurement of oxygen uptake rates is a relatively accurate method to understand cell growth, in case that the traditional cell count method is no longer useful due to heavy cell clumpings. True growth yield, Ymax and maintenance coefficient, me associated with mammalian cell growth were estimated as $2.86{\times}10^8$ cells/ g of glucose and 0.0063 g of glucose/ cells/ hr, respectively.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.28 no.8 s.227
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• pp.910-920
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• 2004

The heat/mass transfer characteristics in a rotating two-pass duct with and without rib turbulators are investigated in the present study. The square duct has a hydraulic diameter ($D_h$) of 26.7 mm, and $1.5\;mm{\times}1.5\;mm$ square $90^{\circ}$-rib turbulators are attached on the leading and trailing walls. The pitch-to-rib height ratio (p/e) is 10. The Reynolds number based on the hydraulic diameter is kept constant at 10,000 to exclude the Reynolds effect, and the rotation number is varied from 0.0 to 0.20. In the smooth duct, the curvature of the $180^{\circ}$-turn produces Dean vortices that enhance heat/mass transfer in the post-turn region. When rib turbulators are installed, heat/mass transfer is augmented 2.5 times higher than that of the smooth duct since the main flow is turbulated by reattaching and separating in the vicinity of the duct surfaces. The duct rotation results in heat/mass transfer discrepancy so that Sherwood number ratios are higher on the trailing surface in the first-pass and on the leading surface in the second-pass. In the turning region, Dean vortices shown in the stationary case transform into one large asymmetric vortex cell, and subsequent heat/mass transfer characteristics also change. As the rotation number increases, the heat/mass transfer discrepancy enlarges.

• Journal of Preventive Medicine and Public Health
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• v.32 no.4
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• pp.427-434
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• 1999

Objectives: This study was conducted to determine, by reviewing the literature, whether treatment of Helicobacter pylori infection in patients with non-ulcer dyspepsia affects symptoms. Methods: We retrieved the literature using MEDLINE search, with nonulcer dyspepsia and Hericobacter pylori and treatment as key words, which were reported from 1984 to 1998, and manual literature search. The criteria for inclusion was as follows; 1) The paper should have confirmed nonulcer dyspepsia as case definition. 2) The paper should have peformed a randomized, blind trial. 3) Confirmation of Helicobacter pylori eradication should be done 4 weeks after treatment. 4) studies with no information on measurement of symptoms after treatment were not accepted. The percentage of patients with symptom improvement after eradication therapy for Helicobacter pylori infection was calculated. Cumulative odds ratio was compared by fixed effect model and random effect model as sensitivity and funnel plot was used to evaluate publication bias. Results: The overall effect size of symptom improvement was calculated by cumulative odds ratio. Cumulative odds ratio of random effect model was 4.16(95% CI: 1.55-11.19). Before integrating each effect sizes into common effect size, the homogeneity test was conducted and random effect model was selected(Cochran's Q=41.08 (d.f=10, p<0.001)). The heterogeneity across studies was evaluated and the different methodological aspects of studies led to differences between study results Conclusions: The results suggest that the eradication of Helicobacter pylori in patients with non-ulcer dyspepsia results more symptom improvement. In studios that shows the opposite results there are methodological aspects explaining the heterogeneity.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.538-541
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• 2005

Tidal flats develop along the south coast ofthe Korean peninsula. These areas are famous for sea farming. Specially, strong and coherent radar backscattering signals are observed over oyster sea farms that consist of artificial structures. Tide height in oyster farm is possible to measure by using interferometric phase and intensity of SAR data. It is assumed that the radar signals from oyster farm could be considered as double-bouncing returns by vertical and horizontal bars. But, detailed backscattering mechanism and polarimetric characteristics in oyster farm had not been well studied. We could not demonstrate whether the assumption is correct or not and exactly understand what the properties of back scattering were in oyster farm without full polarimetric data. The results of AIRSAR L-band POLSAR data, experiments in laboratory and JERS-l images are discussed. We carried out an experiment simulating a target structure using vector network analyser (Y.N.A.) in an anechoic chamber at Niigata University. Radar returns from vertical poles are stronger than those from horizontal poles by 10.5 dB. Single bounce components were as strong as double bounce components and more sensitive to antenna look direction. Double bounce components show quasi-linear relation with height of vertical poles. As black absorber replaced AI-plate in bottom surface, double bounce in vertical pole decreased. It is observed that not all oyster farms are characterized by double bounced scattering in AIRSAR data. The image intensity of the double bounce dominant oyster farm was investigated with respect to that of oyster farm dominated by single bounce in JERS-l SAR data. The image intensity model results in a correlation coefficient (R2 ) of 0.78 in double bounce dominant area while that of 0.54 in single bouncing dominant area. This shows that double bounce dominant area should be selected for water height measurement using In8AR technique.

• Tribology and Lubricants
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• v.33 no.4
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• pp.134-140
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• 2017

With the increase in adoption of tilting pad journal bearings (TPJBs), various failure mechanisms related to TPJBs have been reported, of which pad wear is a frequently reported one. Pad wear causes change in geometry of the bearing, which can sometimes result in the failure of the entire system. The objective of this research is to investigate the influence of pad wear on the pad temperature, which is one of the widely used condition monitoring methods for TPJBs. For the theoretical investigation, thermohydrodynamic (THD) analysis was conducted by solving the generalized Reynolds equation and the 3D energy equation. The results of the analysis show that the temperature of the loaded pad increases while that of the unloaded pad decreases, when there is wear on the loaded pads. In addition, the minimum film thickness decreases with an increase in the wear depth. A validation test was conducted with a test rig, which mimics the axial turbine when a test rotor is supported by two TPJBs. The test bearing consists of five pads with a diameter of 60 mm, and a resistance temperature detector (RTD) is installed in the pad for temperature monitoring. The test was performed by replacing the two loaded pads with the worn pad. The test result for the TPJB with wear depth of $30{\mu}m$ show that the temperatures of the loaded pads are $8^{\circ}C$ higher and that of the unloaded pad is $2.5^{\circ}C$ lower than that of the normal TPJB. In addition, the predicted pad temperature shows good agreement with the measured pad temperatures.