• Title/Summary/Keyword: 2 order system

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Construction of Recombinant Bombyx mori Nuclear Polyhedrosis Virus Using a FLP/FRT System of Yeast, Saccharomyces cerevisiae 2$\mu$m plasmid (Yeast의 FLP/FRT 시스템을 이용한 BmNPV의 유전자 재조합)

  • 강석우;윤은영
    • Journal of Sericultural and Entomological Science
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    • v.40 no.1
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    • pp.52-59
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    • 1998
  • For the construction of plasmid and bmNPV sarrying the FRT recognition site for the FLP recombinases, we synthesized the wild type FRT dligonucleotides. The target FRT sequences consist of three 13bp repeated DNA sequences; two repeats in a direct orientation and one inverted relative to the other two. In addition, there is an 8bp spacer region between the repeats which determune the orientation of the FRT recombination site. In order to place the FRT site both in target BmNPV genome and the transfer vector, we constructed a plasmid, FRT site both in the target BmNPv genome and the transfer vector, we constructed a plasmid, pFRT$\beta$-gal, carrying the FRT sites within the cloning sites of pSV vector and a recombinant BmNPV, vFRTPH, carrying the FRT sites at a downstream of polyhedrin promotor, respectively. In order to test the functionality of the FLP/FRT site-specific recombination system, vFRTPH, pFRT$\beta$-gal and pHsFLP DNA were co-transfected into BmN-4 cells. The resulting recombinant virus was designated a vFRT$\beta$2-gal. From construction analysis of the vFRT$\beta$2-gal with PCR technique it was concluded that the entire pFRT$\beta$-gal plasmid with $\beta$-galactosidase gene and origines of replication flanked by two functional hybrid FRT sequences. The efficiency of recombination was 8.7%, which was higher than that(2.2%) of recombination between a conventional transfer vector and the wild type BmNPV.

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Greenhouse Heating Characteristics of Heat Pump-Latent Heat Storage System (열펌프-잠열축열 시스템의 온실 난방 특성 연구)

  • 강연구;송현갑
    • Journal of Biosystems Engineering
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    • v.25 no.5
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    • pp.379-384
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    • 2000
  • In order to use the natural thermal energy as much as possible for greenhouse heating, the air-air heat pump system involved PCM(phase change material) latent heat storage system was composed, and three types of greenhouse heating system(greenhouse system, greenhouse-PCM latent heat storage system, greenhouse-PCM latent heat storage-heat pump system) were recomposed from the greenhouse heating units to analyze the heating characteristics. The results could be concluded as follows; 1) In the greenhouse heated by the heat pump under the solar radiation of 406.39W/$m^2$, the maximum PCM temperature in the latent heat storage system was 24$^{\circ}C$ and the accumulated thermal energy stored in PCM mass of 816kg during the daytime was 100,320kJ. In the greenhouse without heat pump under the maximum solar radiation of 452.83W/$m^2$, the maximum PCM temperature in the latent heat storage system was 22$^{\circ}C$ and the accumulated thermal energy stored during the daytime was 52.250kJ. 2) In the greenhouse-PCM system without heat pump the heat stored in soil layers from the surface to 30cm of the soil depth was 450㎉/$m^2$. 3) In all of the greenhouse heating systems, the difference between the air temperature in greenhouse and the ambient temperature was about 20~23$^{\circ}C$ in the daytime. In the greenhouse without heat pump and PCM latent heat storage system the difference between the ambient temperature and the air temperature in the greenhouse was about 6~7$^{\circ}C$ in the nighttime, in the greenhouse with only PCM latent heat storage system the temperature difference about 7~13$^{\circ}C$ in the nighttime and in the greenhouse with the heat pump and PCM latent heat storage system about 9~14$^{\circ}C$ in the nighttime.

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Determination of Optimum Timing of Paddy Harvesting Based on Grain Loss and Milling Quality (수호손실과 도정수율을 기초로 한 벼의 수호적기결정에 관한 연구)

  • 강화석;이종호;정창주
    • Journal of Biosystems Engineering
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    • v.2 no.1
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    • pp.56-80
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    • 1977
  • This experimental work was conducted in order to find out the optimum time of harvest of Japonica-type (Akibare) and Indica-type(Tong-il) rice variety for three harvesting systems by investigating the harvesting losses and milling quality. The study was also concerned about the nature and amount of grain losses incurred during the each sequence of post-harvest technologies, and based on these result, a modification of existing systems giving a minimum grain loss was attempted. Binder, combine, and traditional systems were tested in this study and five grain moisture levels were disposed according to the decrease of grain moisture . The results are summarized as follows ; 1. The total losses of Akibare variety were ranged from 1.1 to 1.5 per cent for the traditional harvesting system , 2.1 to 4.8 per cent for the harvesting system by use of binder, and 2.8 to 4.3 per cent for the harvesting system by use of combine as the grain moisture content was reduced from 24 to 15 percent. Milling recovery of the harvesting system by use of binder, 74.8 ∼75.7 percent, was a little higher as it was compared to that of traditional harvesting system, 74.3 ∼75.0per cent, and that of the harvesting system by use of combine, 73.8 ∼75.0 per cent. Head rice recovery of mechanically dried paddy samples was higher than that of sun-dried paddy samples. 2. The total losses of Tong-il variety were ranged from 3.8 to 5.0 per cent for the traditional harvesting system, 5.2 to 10.0 percent for the system by use of binder, and 3.0 to 5.0 per cent for the system by use of combine as the grain moisture was reduced from 28 to 16 per cent. 3. Milling recovery of Tong-il variety harvested by the traditional harvesting system was 72.3 ∼73.6 percent and it was lower when compared to that of 72.3∼75.0 per cent harvested by binder, and 73.0∼74.6 percent harvested by combine. 4. Head rice recovery of Tong-il variety harvested by the traditional harvesting system (58.

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Screening of Anti-Atopic Dermatitis Material by Using NC/Nga Mouse Whole Blood System (NC/Nga 마우스 전혈을 이용한 항 아토피 피부염 물질 탐색)

  • Park, Dong-Hoon;Kim, Youn-Uck
    • IMMUNE NETWORK
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    • v.8 no.3
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    • pp.98-105
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    • 2008
  • Background: Allergic inflammation was induced by activated Th2 lymphocytes, leading to IgE production and eosinophil activation. A Th2 disproportion was shown in atopic children soon after birth. During specific allergen stimulation, an increase of Th2 cells was observed in most cases. In this study, we prepared new screening "whole blood" system for searching the anti-atopic materials. Cytokine production and IgE secretion from whole blood system were assessed and we confirmed the results by using animal system. Methods: Pathological features in NC/Nga mice are similar to those observed in human atopic dermatitis. Whole blood from NC/Nga mouse was stimulated by using TNCB (Th2 activator) or candidate materials of anti-atopic dermatitis, and the production of cytokines (IL-4, IL-12, and IFN-${\gamma}$) were measured by ELISA. In order to confirm the results of whole blood system, in vivo test was done by using NC/Nga mice. Results: In whole blood system, LPS and extracts of green tea, hardy orange and onion induced the production of IL-12 and IFN-${\gamma}$ while they reduced the production of IL-4. Also, LPS and extracts of onion reduced IgE production. Though atopic dermatitis was observed from a mouse stimulated with TNCB, it was not when a mouse was co-stimulated in LPS or extracts of onion. The results are same as those observed in whole blood system. Conclusion: Whole blood system was simple and speedy methods for searching a materials compared with the conventional high-cost animal system. And the results using whole blood system was proved to be reliable in our experiments for screening anti-atopic material. We expect that the system can be applied to other experiments for searching similar materials.

A Study on the Optimization of Nucleation and Crystal Growth in Diopside-Devitrite System (투휘석-Devitrite계 복합용융체의 핵생성 및 결정성장의 최적화에 관한 연구)

  • Ahn, Young-Pil;Oh, Bong-Inn;Choi, Long
    • Journal of the Korean Ceramic Society
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    • v.16 no.3
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    • pp.135-141
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    • 1979
  • This study seeks to find optimum conditions for the heating schedule of the Diopside-Devitrite system, to find the amounts and the kinds of nucleus which effect the crystal growth and forming nucleus. Generally, crystallization in the glass depends on the number of nucleus growing in the internal system and the rate of crystal growth. In order to obtain homogeneous polystalline phae, Diopside as MgO source and $ZrO_2$.$P_2O_5$, $TiO_2$, NaF, $CaF_2$ as nucleating agents were added to the $Na_2O$.CaO.$6SiO_2$ glass. The results obtained were Summarized as follows. 1) Optimum Batch Composition of base glass is 76.82 wt.% $SiO_2$, 5.84 wt.% CaO, 4.54 wt.% MgO and 9.80 wt.% $Na_2O$. 2) Best heating schedule.140$0^{\circ}C$(Melting)coolinglongrightarrow95$0^{\circ}C$reheatinglongrightarrow$1100^{\circ}C$coolinglongrightarrowRoom Temp. 3) The optimum amounts of $ZrO_2$.$P_2O_5$, $TiO_2$ and $CaF_2$ are 3wt.% and that of NaF is 4 wt.% as a nucleating agents.

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A Performance Comparison of CR-MMA and CM-MMA Equalization Algorithm in 2-dimensional QAM System (2차원 QAM 시스템에서 CR-MMA 와 CM-MMA 등화 알고리즘의 성능 비교)

  • Lim, Seung-Gag
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.4
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    • pp.85-90
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    • 2018
  • This paper compares the adaptive eualization performance of CR-MMA (Constellation Reduction-MMA) and CR-MMA (Constellation Matching-MMA) in order to minimization of intersymbol interference that is occur in channel for 2-dimensional QAM signal. For obtain the error signal in order to updating the tap coefficient of conventional adaptive equalization algorithm MMA, the CR-MMA converts the constellation reduction concept of high order 2-dimensional QAM nonconstant modulus signal to constant modulus signal and the CM-MMA use the constellation matchine error concept in order to force the matching the $2^{nd}$ and $4^{th}$ cumulant of equalizer output and transmitted signal constellation. By applying the different method for getting the error signal, these algorithm are possible to obtain the improved equalization performance compared to the conventional MMA algorithm, the improved performance of CR-MMA and CM-MMA were compared by computer simulation in this paper. As a result of simulation, the CM-MMA has more better performance in the equalizer internal performance than CR-MMA, but not in equalizer external performance as in SER.

Ecosystem Structure and Improvement of Naturalness in Urban Area -In the Case of Kangseo-gu in Seoul- (도시생태계 현황파악 및 자연성 증진 방안 -서울시 강서구를 사례로-)

  • 이수동;이경재
    • Journal of the Korean Institute of Landscape Architecture
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    • v.32 no.3
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    • pp.1-17
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    • 2004
  • The focus of this study is the promotion of green area volumes and their naturalness, water circulation system, decline of entropy, creation of biological habitats and linkage of separated urban green space. Re-presentative urban biotope survey sites were categorized as urban biotope, semi-natural biotope, and natural forest. In the urban biotope, a residential biotope was constructed near the Han river and in mountain areas. The green-area ratio at the housing complex was about 25%. GVZ(Grunvolumenzahl) was 0.35m$^3$/m$^2$ at the 5∼10-story housing complex, and 1.53m$^3$/m$^2$ over the 11-story. As for the green-area structure of the housing complex, canopy layer, understory layer, and shrub layer were not differentiated and the green-area volume was not high enough. The green-area ratio of school areas as a public area biotope was 5∼20%. GVZ was 1.12m$^3$/m$^2$ at Myungduk High School, and 1.78m$^3$/m$^2$ at Jeonggok Elementary School. In order to convert the urban biotope into an ecological area, green areas around the buildings should be connected to urban buffer green areas, and multi-layer structures should be established with natural plant species. In the semi-natural biotope, neighbor parks were created park in the vicinity of the natural forests. GVZ was 0.28m$^3$/m$^2$, and plantation was established with single layer structure and was definitely insufficient for the area. The urban buffer green areas have been established in strip corridors with the width of 20∼123m. In those areas, GVZ was 0.16∼0.27m$^3$/m$^2$ and had a deficient canopy layer, understory layer, and shrub layer. Soil conditions were not favorable for tree growth. In the natural biotope, GVZ of the plantation was 1.03∼1.5m$^3$/m$^2$ but the high crown closure of this area reduces the chance of species change and succession. GVZ of natural forest was 2.53∼2.57m$^3$/m$^2$. It is desirable to plant diverse plants and the natural forest should be succeeded by broad-leaf deciduous tree species. To improve the value of biotope at Kangseo-Gu, building height needs to be limited to reduce the environmental deterioration in the city. In order to maintain the water circulation system, water-permeable material is recommened when the urban surface areas are paved. The establishment of a water circulation system will improve ground water levels, soil moisture, water quality, and habitats. In order to improve biological diversity, it is desirable to have multi-layer structures in urban green areas with native species.

A Semi-graphical Analysis on the Sublattice Anisotropy of a Two Sublattice System with Uniaxial Anisotropy: Application to Pr2Fe14B

  • Kim, Y.B.;Jin-Han-Min
    • Journal of Magnetics
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    • v.3 no.3
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    • pp.74-77
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    • 1998
  • A method to analyze the anisotropy constants of a two sublattice system with uniaxial anisotropy has been investigated by extending the Sucksmith and Thompson's method to higher order anisotropy terms. Using the method, a set of anisotropy constants for Pr-sublattice of $Pr_2Fe_{14}B at 4.2K has been obtained as K_{1Pr}=5210 J/kg, K_{2Pr}=-7200 J/kg, K_{3Pr}=-770 J/kg, K_{4Pr}=4940 J/kg and K_{SPr}=700 J/kg for N_{PrFe}=2.2 T/Am^2kg^{-1}.$ The magnetization calculated by an energy minimum method by using the sublattice anisotropy constants well reproduced the experimental results and satisfied the simulation assumptions.

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Zeroth-Order Resonant Antenna with Frequency Reconfigurable Radiating Structures (주파수 재구성 가능한 방사 구조를 갖는 영차 공진 안테나)

  • Lee, Hongmin
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.9
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    • pp.12-20
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    • 2013
  • In this paper, a co-planar waveguide(CPW) fed zeroth-order resonant(ZOR) antenna with frequency reconfigurable radiating structures is fabricated and measured. The unit cell of proposed antenna consists of a series metal-insulator-metal(MIM) capacitor and two shunt line inductors which are shorted through the via. The proposed antenna is designed based on a composite right/left-handed(CRLH) transmission line with two unit cells and it has open ended structure in order to radiate electromagnetic energy mainly on the shunt arm. In order to reduce the antenna size and to exhibit a frequency reconfigurable ability using diode switches four straight strips bent by 90 degrees are used as shunt inductors. The total size of fabricated antenna is $0.22{\lambda}_0{\times}0.16{\lambda}_0$ at zeroth-order resonant(ZOR) frequency. The measured maximum gain and bandwidth (VSWR ${\leq}2$) are 3.1 dBi and 56MHz at ZOR frequency of 2.97 GHz, respectively. This type of antenna can be applied to a frequency reconfigurable antenna system with triple bands.

Synthesis of Spinel Pigment on ZnO-Fe2O3 System (ZnO-Fe2O3계 Spinel안료에 대한 연구)

  • 이진성;이응상
    • Journal of the Korean Ceramic Society
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    • v.26 no.2
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    • pp.187-194
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    • 1989
  • Synthesis of spinel pigment on ZnO-Fe2O3 system. The object of this research is the synthesis of new spinel pigments on the basic of ZnO-Fe2O3 system which was substituted by ZnO-Fe2O3 by MgO-Al2O3. This research was progressed by measuring the X-ray diffraction and the reflectances of the substitued ZnO-Fe2O3 group. Which was obtained by sintering at the temperature of 1,00$0^{\circ}C$, 1,10$0^{\circ}C$, 1,20$0^{\circ}C$ and 1,25$0^{\circ}C$ and them by regrinding. In order to coloring test, here basic compositions of Barium glaze, Zinc glaze, Lime glaze, Lead glaze and Talc glaze used in this experiment are obtained from the ceramic work. Adding synthetic stains in these basic glazes with 3%, mixing and glazing on the specimen. The specimens was fired at 1,28$0^{\circ}C$ in reducing and oxidizing atmosphere in the gas kiln. The results of the research as follow. 1. Many kinds of spinel pigment was produced on ZnO-Fe2O3 system that is to say, not always only spinel. 2. Spinel peak was observed strongly on the ZnO-Fe2O3 system withsubstituting by MgO-Fe2O3 and MgO-Al2O3 group(the ratio of MgO, Al2O3 being increased, observed more strongly). 3. The most effective temperature ranges was 1,20$0^{\circ}C$~1,25$0^{\circ}C$. 4. The color of spinel pigments on this system was observed by "stable YR". 5. It was yellow red in oxidizing and green in reducing atmosphere on the coloring test.ring test.

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