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Hormonal Regulation of Insulin-Like Growth Factor Binding Protein Secretion by a Bovine Mammary Epithelial Cell Line

  • Kim, W.Y.;Chow, J.C.;Hanigan, M.D.;Calvert, C.C.;Ha, J.K.;Baldwin, R.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.2
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    • pp.233-239
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    • 1997
  • A mammary epithelial cell line (MAC-T) established as a model for lactation was utilized to identify and characterize effects of various hormones upon insulin-like growth factor binding protein secretion. Ligand and immunoblot analyses of conditioned media indicated that insulin-like growth factor binding protein-2 was secreted by MAC-T cells. Insulin-like growth factor-I stimulated insulin-like growth factor binding protein-2 secretion in a dose-dependent manner, but prolactin and bovine somatotropin did not alter insulin-like growth factor binding protein-2 secretion. Insulin increased and cortisol decreased insulin-like growth factor binding protein-2 secretion. Effects of insulin-like growth factor-I on insulin-like growth factor binding protein-2 secretion support previous studies using primary cultures of bovine mammary cells and bovine fibroblasts. Effects of cortisol and insulin on insulin-like growth factor binding protein-2 secretion may be explained by changes in protein synthesis. In addition, supraphysiological doses of insulin can cross-react with the insulin-like growth factor-I receptor and stimulate insulin-like growth factor binding protein-2 secretion. MAC-T cells provide a model system to study mechanisms that regulate local insulin-like growth factor-I bioactivity.

The Effect of Oxygen Transfer Rate on the Nebramycin Factor 5' Activity and Component Ratio in Streptoalloteichus hindustanus Fermentation. (Streptoalloteichus hindustanus 발효시 Nebramycin Factor 5' 역가 및 구성비율에 대한 산소전달속도의 영향)

  • 김정근;이병규;노용택
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.395-399
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    • 2003
  • Nebramycin is a complex of aminocyclitol compounds that is produced by aerobic culture in fermentation process. The major antibiotic factors produced by Streptoalloteichus hindustanus are nebramycin factor 2, 4, 5'and kanamycin A. A mutant was selected, producing nebramycin factor 5' activity 16.4 times higher than parent strain by microbiological assay using Pseudomonas aeruginosa CH-U34AF. The component ratio of nebramycin factor 5' was dramatically increased from 34% to 70% by the optimization of fermentation condition. It was found that the component ratio of nebramycin factor 5' in fermentation was especially affected by the oxygen transfer rate. Optimum oxygen transfer rate for maximal nebramycin factor 5' productivity and ratio during S. hindustanus fermentation was elucidated to $0.50 mMO_2$/min.

The Sense of Touch of Man-made Leather (인조피혁의 촉감평가)

  • 이정순;신혜원
    • Journal of the Korean Society of Clothing and Textiles
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    • v.24 no.2
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    • pp.277-285
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    • 2000
  • The purpose of this study was to quantify the relationship between the sense of touch and mechanical properties of man-made leather. The first was to develop the five conversion equations which convert mechanical properties of man-made leather into five factor scores, which express five factors of the sense of touch(surface property, stretchiness, thickness & weight, thermal property(warmth & coolness), and moisture property(sticky & clingy)). The second was to develop the conversion equation which converts five factor scores into score of the sense of touch. Five factor scores were predicted by the following mechanical properties; surface property factor by log2HB and (log2HB)2, stretchiness factor by logEM, thickness & weight factor by logT, log2HB, logW, thermal property factor by logT, logHB, logSMd, and moisture property factor by logMMD, RC, RC2, (logEM)2, RT2. Subsequently, these five factor scores were converted into score of the sense of touch. The predictive abilities of the developed equations were satisfied.

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A Study on the Relationships between the Attitudes of University Students toward Money and the Attributes of Choosing Family Restaurants (대학생의 금전에 대한 태도에 따른 패밀리 레스토랑 선택 속성에 관한 연구)

  • Jung, Hyo-Sun;Yoon, Hye Hyun
    • Culinary science and hospitality research
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    • v.14 no.2
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    • pp.99-114
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    • 2008
  • The purpose of this study is to examine the relationships between each type of attitudes toward money and the attributes of choosing family restaurants. A self-administrated questionnaire was completed by 387 students, and data were analysed by frequency, factor, reliability and canonical correlation. Seven factors were obtained from factor analysis of attitudes toward money; Factor 1 "power", Factor 2 "obsession", Factor 3 "retention", Factor 4 "achievement", Factor 5 "anxiety", Factor 6 "distrust", and Factor7 "evaluation". The attributes of family restaurant choice were extracted into six factors: Factor 1 "quality of food", Factor 2 "restaurant event", Factor 3 "interior environment", Factor 4 "value of food", Factor 5 "convenience for approach", and Factor 6 "employees' service". Canonical correlation analysis showed three significant functions. Canonical function 1 showed that the attitudes of considering the power of money, its retention and achievement were indicated to have significantly positive relationships with the quality of food in the attributes of choosing family restaurants. Canonical function 2 showed that significantly negative relationships between distrust and restaurant events and convenience for approach. Canonical function 3 also showed that significantly positive relationships between obsession and anxiety and the interior of restaurants and employees' service, and significantly negative relationships between evaluation and the interior of restaurants and employees' service.

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Visual Evaluation to the types of Set-in Sleeve (Set-in Sleeve의 형태에 따른 시각적 평가)

  • Kim, Ye-Kyung;Lee, Young-Ju
    • Korean Journal of Human Ecology
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    • v.17 no.2
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    • pp.305-313
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    • 2008
  • This subject investigated visual evaluation of preference and fitness level of set-in sleeve in sleeve types(form)and studied suitable sleeve type at silhouette and targeted female students in twenties. The results of study were as follows: 1. Visual evaluation factors in set-in sleeve of sleeve types were selected 6 factors; the 1st factor is decorativeness, the 2nd factor is attractiveness, the 3rd factor was activeness, the 4th factor was feminine, the 5th factor was charming and 6th factor was a figural element. Decorativeness and attractiveness factors in visual evaluation of sleeve types were the most important level. 2. The visual evaluation differences of sleeve types in set-in sleeve were as follows; in the 1st factor, S and P2 were the most decorative sleeve type. in the 2nd factor, S, P2 and P3 are the most attractive sleeve type. in the 3rd factor, B and P3 were the most active sleeve type. in the 4th factor, L was the most feminine sleeve type. in the 5th factor, P1, L and P3 were charming sleeve type. in the 6th factor, P3 was evaluated that it shows narrowened shoulder sleeve types. 3. The analysis result of preference and wearing level in each sleeve types were as follows. 20's female university students like to wear S, P1, P2 and P3 sleeve types and the most favorite sleeve types are S, P2 and P3. 4. The analysis result of silhouette set-in sleeve in sleeve types; S, B and P2 in H-type silhouette, P2, S and P1 in A-type silhouette, B and P3 in O-type silhouette, L and P3 in X-types silhouette were show the most suitable sleeve types.

Improved Method for Indeterminate forces of Two-span Steel-concrete Composite Girders (2경간 연속 강합성거더의 개선된 부정정력 계산 방법)

  • Ji, Gu Sam;Kim, Choong Eon;Shin, Dong Ki;Choi, Dong Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.2
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    • pp.423-431
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    • 2013
  • The k-factor method has been widely used in design of steel-concrete composite bridges to determine indeterminate stresses. The accuracy of k-factor method is examined by a comparative analysis with the equivalent load method in a continuous 2-span composite bridge. To improve the accuracy, the direction of creep stress has to be modified and the variety of section must be considered. This paper suggests j-factor method which can improve the accuracy of k-factor method with simple modification.

Co-relation between Questionnaire for Buzhongyiqi-Tang and Electrogastrography (보중익기탕 변증설문지와 위전도의 상관성 연구)

  • Kim, Sun-Hyeok;Park, Young-Bae;Park, Young-Jae;Oh, Hwan-Sup
    • The Journal of the Society of Korean Medicine Diagnostics
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    • v.13 no.2
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    • pp.34-44
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    • 2009
  • Background : Currently, as a method of standardization of prescription, questionnaire for Buzhongyiqi-Tang[補中益氣湯] was developed, and which is a Objectives : The purpose of this thesis is to testify whether differentiation of Lao Juan Shang[勞倦傷] etiology is relative to mobility of gastric smooth muscle. Methods : The subjects(20 to 65 years old; 14 males, 46 females) were isolated from drinking alcohols for 24 hours before the experiment, and fasted for 8 hours, and measured for electrogastrography(EGG) and they filled out Questionnaire for Buzhongyiqi-Tang. Results : 1. Six factors from the factor analysis of Questionnaire for Buzhongyiqi -Tang were named and classified as Spleen-Qi deficiency syndrome factor [脾虛] (factor 1), Lung-Qi deficiency syndrome factor [肺虛] (factor 2), Working factor [習慣] (factor 3), Yin-Fire factor [陰火] (factor 4), Jung-Qi deficiency syndrome factor [中氣虛] (factor 5), and Stomach-Qi deficiency syndrome factor [胃虛] (factor 6). 2. As for the reliability of Questionnaire for Buzhongyiqi-Tang, we used Cronbach's alpha coefficient. Cronbach's alpha coefficient was 0.772 for the mean of the item-total. 3. Lung-Qi deficiency syndrome factor(factor2) had significant correlation with Bradygastria Time (r=-0.312, p<0.05). 4. Working factor(factor3) had significant correlation with Bradygastria Time (r=-0.329, p<0.05). 5. Yin-Fire factor(factor4) had significant correlation with Power Ratio (r=-0.328, p<0.05). Conclusions : It is shown that Bradygastria Time and Power Ratio tended to decrease against postprandial DP increased and postprandial frequency decreased in normal case.

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An Empirical Analysis on Public Transportation Demand and TOD Design Factors in Seoul subway adjacent area (서울시 역세권의 TOD환경과 대중교통이용수요 관계분석)

  • Moon, Young-Il;Rho, Jeong-Hyun
    • International Journal of Highway Engineering
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    • v.13 no.4
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    • pp.211-220
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    • 2011
  • TOD(Transit Oriented Development) has recently been active, which presents that TOD planning elements should be comprehensively taken into consideration in order to enhance domestic transit ridership by changing environments in rail station areas and an empirical analysis on the type of rail station areas and transportation demand should be a prerequisite for usage of future development planning. This study aims to grasp a variety of TOD of influence factors in Seoul rail station area and to perform analysis to identify relationship between public transportation demand and these TOD design factors. To make it come true, we gathered data with respect to Density, Diversity, and Accessibility as representative TOD planning elements and carried out factorial and regression analysis. Consequently, we drew 7 influence factors base on factorial analysis: Factor 1(Diversity/ -Use Mix(LUM)), Factor 2(Density/development density), Factor 3(Accessibility/public transportation facility supply), Factor 4(Design/street design), Factor 5(Green/access mode (pedestrian, bike), Factor 6(Design/subway size), Factor 7(Accessibility/Public transit operation) As the result of model development by using factorial and regression analysis, positive influence factors on passenger flow in rail station area are Factor 1(Diversity : Land-Use Mix), Factor 3(Accessibility : public transportation facility supply), Factor 2(Density : development density), Factor 5(Design/ access mode) and Factor 6(subway size) Next, negative influence factor on passenger flow in rail station area shows Factor 7(Accessibility/Public transit operation) as the most influential factor. This is because the growth of service interval of linked subway and bus leads to reduced demand.

Role of Nuclear Factor Erythroid 2-Related Factor 2 in Chronic Obstructive Pulmonary Disease

  • Ban, Woo Ho;Rhee, Chin Kook
    • Tuberculosis and Respiratory Diseases
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    • v.85 no.3
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    • pp.221-226
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    • 2022
  • Chronic obstructive pulmonary disease (COPD) is characterized by airflow limitation due to chronic airway inflammation and destruction of the alveolar structure from persistent exposure to oxidative stress. The body has various antioxidant mechanisms for efficiently coping with such oxidative stress. The nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) is a representative system. Dysregulation of the Nrf2-ARE pathway is responsible for the development and promotion of COPD. Furthermore, COPD severity is also closely related to this pathway. There has been a clinical impetus to use Nrf2 for diagnostic and therapeutic purposes. Therefore, in this work, we systematically reviewed the clinical significance of Nrf2 in COPD patients, and discuss the value of Nrf2 as a potential COPD biomarker.

A Study on Ion Distribution and Behavior of Acidrain in Mokpo and Yeochon Area (목포, 여천지역 강수의 무기이온 성분농도와 거동에 관한 연구)

  • 오길영;양수인;이완진
    • Journal of Korean Society for Atmospheric Environment
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    • v.15 no.4
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    • pp.385-392
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    • 1999
  • This study was carried out to investigate the constituents of inorganic ions of precipitation in Mokpo and Yeochon area from Jan. 1996 to Dec. 1997. The volume-weighted mean pH of precipitation was 5.7(4.8~7.8) at Mokpo, and 5.6(5.1~7.4) at Yeochon area, respectively. The non-seasalt(nss) anion concentrations were found in order of $nss-SO^{2-}_4>NO^-_3>nss-Cl^-$ at two areas. The portion of $nss-SO^{2-}_4;and;NO^-_3$ was 71~84% out of anions. The the non-seasalt cation concentrations were found in order of $NH^+_4>nss-Ca^{2-}>nss-Mg^{2+}>nss-K^-$ at two areas. The portion of $NH^+_4;and; nss-Ca^{2+}$ was 85~92% out cations. Compared regionally the year concentraton of $nss-SO^{2-}_4$, the result of Yeochon was 3 times higher than that of Mokpo in 1996, while the results of two regions were almost similar in 1997. The reason was that Yeochon was restricted area of the use of 0.5% sulfur B-C oil, controlled air pollution emission area, controlled semitotal amounts of air pollution emission area and partial operating of factories in 1997. There were no ions having high correlation with $H^+,;but;nss-SO^{2-}_4,;NO^-_3,;NH^+_4;and;nss-Ca^{2+}$ showed high correlation coefficient each other. It seems that these ions have little correlation with $H^+$ because they are washed out on binding state. Factor analysis showed that the first factor was complicated factor containing anthropogenic and soil resource, the second factor was sea-salt resource and the third factor was independent behavior of hydrogen at Mokpo. While, the first factor was complicated factor containing anthropogenic and sea-salt resource, the second factor was $Ca(NO_3)_2$ salt and the third factor was $NH^+_4;and;SO^{2-}_4$ synergied by resource and combination at Yeochon.

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