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GENOTYPE (BREED) AND ENVIRONMENT INTERACTION WITH PARTICULAR REFERENCE TO CATTLE IN THE TROPICS - Review

  • Vercoe, J.E.;Frisch, J.E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.3
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    • pp.401-409
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    • 1992
  • Genotype $\times$ environment (G $\times$ E) interactions must be understood if they are to be exploited to improve animal production, particularly in production systems associated with large environmental variations. The measurement and evaluation of G $\times$ E are discussed. Examples are presented that demonstrate G $\times$ E in different breeds of beef cattle for high temperatures, internal and external parasites and changes in quantity and quality of nutrition. It is demonstrated that productivity differences between genotypes or breeds under grazing conditions arise because of differences between genotypes in the combination of production potential and resistance to environmental stresses in relation to the levels of the relevant environmental stresses that are operating at the time. The $F_1$ cross between genotypes with high production potential (e.g. European Bos Taurus breeds) and those with high resistance to environmental stress (e.g. Asian and African Bos indicus and sanga breeds) is an exceptional genotype with a unique combination of these two sets of attributes. The principles for G $\times$ E developed for beef cattle are briefly discussed in relation to dairy cattle, pigs, poultry and buffalo.

Urinary Mercury Levels Among Workers in E-waste Shops in Nakhon Si Thammarat Province, Thailand

  • Decharat, Somsiri
    • Journal of Preventive Medicine and Public Health
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    • v.51 no.4
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    • pp.196-204
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    • 2018
  • Objectives: To determine urinary mercury levels in e-waste workers in Southern Thailand and the airborne mercury levels in the e-waste shops where they worked, to describe the associations between urinary and airborne mercury levels, and to evaluate the prevalence of mercury exposure-related health effects among e-waste workers. Methods: A cross-sectional study was conducted by interviewing 79 workers in 25 e-waste shops who lived in Nakhon Si Thammarat Province, Thailand. Information on general and occupational characteristics, personal protective equipment use, and personal hygiene was collected by questionnaire. Urine samples were collected to determine mercury levels using a cold-vapor atomic absorption spectrometer mercury analyzer. Results: The e-waste workers' urinary mercury levels were $11.60{\mu}5.23{\mu}g/g$ creatinine (range, 2.00 to $26.00{\mu}g/g$ creatinine) and the mean airborne mercury levels were $17.00{\mu}0.50{\mu}g/m^3$ (range, 3.00 to $29.00{\mu}g/m^3$). The urinary and airborne mercury levels were significantly correlated (r=0.552, p<0.001). The prevalence of self-reported symptoms was 46.8% for insomnia, 36.7% for muscle atrophy, 24.1% for weakness, and 20.3% for headaches. Conclusions: Personal hygiene was found to be an important protective factor, and should therefore be stressed in educational programs. Employers should implement engineering measures to reduce urinary mercury levels and the prevalence of associated health symptoms among e-waste workers.

SOME PROPERTIES OF THE GENERALIZED GOTTLIEB GROUPS

  • Yoon, Yeon Soo
    • Journal of the Chungcheong Mathematical Society
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    • v.14 no.1
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    • pp.1-6
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    • 2001
  • We investigate the relationships between the Gottlieb groups and the generalized Gottlieb groups, and study some properties of the generalized Gottlieb groups. Lee and Woo [5] proved that $G_n(X,i_1,X{\times}Y){\simeq_-}G_n(X){\oplus}{\pi}_n(Y)$. We can easily re-prove the above main theorem of [5] using some properties of the generalized Gottlieb groups, and obtain a more powerful result as follows; if $F{\rightarrow}^iE{\rightarrow}^pB$ is a homotopically trivial fibration, then $G_n(F,i,E){\simeq_-}{\pi}_n(B){\oplus}G_n(F)$.

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A Comparative Study on Quantifying Uncertainty of Vitamin A Determination in Infant Formula by HPLC (HPLC에 의한 조제분유 중 비타민 A 함량 분석의 측정불확도 비교산정)

  • Lee, Hong-Min;Kwak, Byung-Man;Ahn, Jang-Hyuk;Jeon, Tae-Hong
    • Korean Journal of Food Science and Technology
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    • v.40 no.2
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    • pp.152-159
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    • 2008
  • The purpose of this study was to determine the accurate quantification of vitamin A in infant formula by comparing two different standard stock solutions as well as various sample weights using high performance liquid chromatography. The sources of uncertainty in measurement, such as sample weight, final smaple vloume, and the instrumental results, were identified and used as parameters to determine the combined standard uncertainty based on GUM(guide to the expression of uncertainty in measurement) and the Draft EURACHEM/CITAC Guide. The uncertainty components in measuring were identified as standard weight, purity, molecular weight, dilution of the standard solution, calibration curve, recovery, reproducibility, sample weight, and final sample volume. Each uncertainty component was evaluated for type A and type B and included to calculate the combined uncertainty. The analytical results and combined standard uncertainties of vitamin A according to the two different methods of stock solution preparation were 627 ${\pm}$ 33 ${\mu}$g R.E./100 g for 1,000 mg/L of stock solution, and 627 ${\pm}$ 49 ${\mu}$g R.E./100 g for 100 mg/L of stock solution. The analytical results and combined standard uncertainties of vitamin A according to the various sample weighs were 622 ${\pm}$ 48 ${\mu}$g R.E./100 g, 627 ${\pm}$ 33 ${\mu}$g R.E./100 g, and 491 ${\pm}$ 23 ${\mu}$g R.E./100 g for 1 g, 2 g, and 5 g of sampling, respectively. These data indicate that the preparation method of standard stock solution and the smaple amount were main sources of uncertainty in the analysis results for vitamin A. Preparing 1,000 mg/L of stock solution for standard material sampling rather than 100 mg, and sampling not more than 2 g of infant formula, would be effective for reducing differences in the results as well as uncertainty.

Medium Optimization for Phytase Production by Recombinant Escherichia coli Using Statistical Experimental Design

  • Choi, Won-Chan;Oh, Byng-Chul;Kim, Hyung-Kwoun;Lee, Eun-Sook
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.490-496
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    • 2002
  • The production of E. coli WC7 phytase from a recombinant E. coli strain was optimized using a statistical experimental design approach. Two-level complete factorial designs with seven variables were used for the media optimization. In the first optimization step, the influence of disodium succinate, yeast extract, $K_2HPO_4,\;NH_4H_2PO_4,\;MgSO_4$, NaCl, and trace elements on phytase production was evaluated. As a result, disodium succinate, yeast extract, $NH_4H_2PO_4$, NaCl, and the trace elements were found to have a positive influence on the phytase production, while $K_2HPO_4\;and\;MgSO_4$ had a negative influence. In the second step, the concentrations of disodium succinate and yeast extract were further optimized using central composite designs. The maximum phytase activity obtained was 234 U/ml using 15.9 g/1 disodium succinate, 20 g/1 yeast extract, 5 g/1 K_2HPO_4,\;10 g/1 NH_4H_2PO_4,\;1.5 g/1 MgSO_4$, 4 g/1 NaCl, and 1.5 m1/1 trace elements, which was about a 14-fold increase in comparison with that obtained using the basal medium.

THE CLASSIFICATION OF COMPLETE GRAPHS $K_n$ ON f-COLORING

  • ZHANG XIA;LIU GUIZHEN
    • Journal of applied mathematics & informatics
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    • v.19 no.1_2
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    • pp.127-133
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    • 2005
  • An f-coloring of a graph G = (V, E) is a coloring of edge set E such that each color appears at each vertex v $\in$ V at most f(v) times. The minimum number of colors needed to f-color G is called the f-chromatic index $\chi'_f(G)$ of G. Any graph G has f-chromatic index equal to ${\Delta}_f(G)\;or\;{\Delta}_f(G)+1,\;where\;{\Delta}_f(G)\;=\;max\{{\lceil}\frac{d(v)}{f(v)}{\rceil}\}$. If $\chi'_f(G)$= ${\Delta}$f(G), then G is of $C_f$ 1 ; otherwise G is of $C_f$ 2. In this paper, the classification problem of complete graphs on f-coloring is solved completely.

Effect of Continuous Exposure to Reactive Oxygen Species on ${\gamma}$-Glutamyltranspeptidase Expression and Activity in HepG2 Cells (HepG2 세포에서 지속적인 활성 산소 노출이 ${\gamma}$-Glutamyltranspeptidase 발현과 활성에 미치는 영향)

  • Kim, Young-Whan;Choe, Dal-Ung
    • Journal of Environmental Health Sciences
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    • v.30 no.3
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    • pp.230-238
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    • 2004
  • The adverse health effects of a number of environment pollutions are related to the formation of free radicals. Induction of antioxidant defensive system in the response to an oxidative attack is an essential element of the cell to survive. CYP2E1 is easily induced by organic solvents and induces continuous formation of reactive oxygen species (ROS). ${\gamma}$-Glutamyltranspeptidase (${\gamma}$GT) plays an important role in glutathione metabolism and xenobiotic detoxification. To evaluate the characteristic of oxidative stress which induces GGT expression and to understand human antioxidant defensive response against oxidative stress induced by CYP2E1, we studied regulation of ${\gamma}$GT enzyme expression in response to various oxidative stresses in human HepG2 cells. The ${\gamma}$GT activity was not modified after exposure of acute oxidative stress inducing agents (ferric nitrilotriacetate, cumene hydroperoxide, ADP-Fe, O-tetradecanoylphorbol-13-acetate, tumor necrosis factor-alpha). To induce continuous exposure of cells to ROS, HepG2 cells were transfected by human CYP2E1 gene transiently. The CYP2E1 activity was verified with chlorzoxazone hydroxylation. Transfection of CYP2E1 showed continuous 60% increase in intracellular ROS and 240 % increase in microsomal ROS. CYP2E1 overexpressing cells showed increased ${\gamma}$GT activity (2.5-fold). The observed enhancement of ${\gamma}$GT activity correlated with a significant increase of ${\gamma}$GT mRNA (2.1-fold). Treatment with antioxidant strongly prevented the increase in ${\gamma}$GT activity. The CYP2E1 overexpression did not modify toxicity index and increased glutathione levels. These results show that continuous exposure of cells to ROS produced by CYP2E1 up-regulates ${\gamma}$GT; This may be one of the adaptive antioxidant responses of cells to oxidative insult. Present study also suggests that the induction of ${\gamma}$GT could be used as a marker of oxidative stress induced by exposure to organic solvents.

Nannochloris eucaryotum growth: Kinetic analysis and use of 100% CO2

  • Concas, Alessandro;Lutzu, Giovanni Antonio;Locci, Antonio Mario;Cao, Giacomo
    • Advances in environmental research
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    • v.2 no.1
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    • pp.19-33
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    • 2013
  • Microalgae are receiving an increasing attention because of their potential use as $CO_2$ capture method and/or as feedstock for biofuels production. On the other hand the current microalgae-based technology is still not widespread since it is characterized by technical and economic constraints that hinder its full scale-up. In such contest the growth kinetics of Nannochloris eucaryotum (a relatively unknown marine strain) in batch and semi-batch photobioreactors is quantitatively investigated with the aim of obtaining the corresponding kinetic parameters suitable for process engineering and its optimization. In particular the maximum growth rate was evaluated to be 1.99 $10^{-3}\;h^{-1}$. Half saturation concentrations for nitrates ($K_N$) and phosphates uptake ($K_P$) were evaluated as 5.4 $10^{-4}\;g_N\;L^{-1}$ and 2.5 $10^{-5}\;g_P\;L^{-1}$, respectively. Yield factors for nitrogen ($Y_N$) and phosphorus ($Y_P$) resulted to be 5.9 $10^{-2}\;g_N\;g^{-1}$ biomass and 6.0 $10^{-3}\;g_P\;g^{-1}{_{biomass}}$, respectively. The possibility of using 100% (v/v) $CO_2$ gas as carbon source is also evaluated for the first time in the literature as far as N. eucaryotum is concerned. The strain showed a good adaptability to high concentrations of dissolved $CO_2$ as well as to low pH. The lipid content under 100% $CO_2$ is about 16.16 %wt $wt^{-1}$ and the fatty acid methyl esters composition of the extracted oil is in compliance with the European regulation for quality biodiesel.

Genotypic Difference in Tocopherol and Tocotrienol Contents of Rice Bran (벼 품종별 미강의 Tocopherol과 Tocotrienol 함량 평가)

  • 박경열;강창성;조영철;이용선;이영현;이영상
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.469-472
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    • 2003
  • The rice bran has been known to contain tocopherols and tocotrienols carrying antioxidant and cholesterol-lowing activities. The content of 8 isomers of vitamin E : $\alpha\textrm{-}$, $\beta\textrm{-}$, $\gamma\textrm{-}$, $\delta\textrm{-}$tocoperols (T) and tocotrienols ($\textrm{T}_3$) were extracted from 18 major rice varieties and quantified with an HPLC. Tested varieties exhibited T, $\textrm{T}_3$ and total vitamin E ($\textrm{TT}_3$) contents ranging 9.1-14.8, 22.4-37.1, 34.9-46.5 mg/100g with averages of 11.1, 28.0, 39.2 mg/100g, respectively. Among tested varieties, Seojinbyeo and Hwasungbyeo showed high T contents and Andabyeo, Damakum were high in $\textrm{T}_3$, and Andabyeo and Seojinbyeo were high in total $\textrm{TT}_3$ contents. Regardless of varieties, the average 8 isomer contents (in mg/100g) were in descending order of $\gamma\textrm{-}$$\textrm{T}_3$(17.9) >$\alpha\textrm{-}$$\textrm{T}_3$(8.8) >$\alpha\textrm{-}$T (7.8) >$\gamma\textrm{-}$T(2.6) >$\delta\textrm{-}$$\textrm{T}_3$ (0.9)>$\beta\textrm{-}$T (0.7)>$\beta\textrm{-}$$\textrm{T}_3$ (0.4)>$\delta\textrm{-}$T (0.1). In most varieties, $\gamma\textrm{-}$$\textrm{T}_3$, a strong antioxidant and anticancer compound, consisted 64% of total tocotrienol and 46% of total vitamin E in vice bran.

Cottonseed Extract Improves the Function of Osteoblastic MC3T3-E1 Cells

  • Choi, Eun-Mi;Lee, Kyung-Hee
    • Food Quality and Culture
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    • v.2 no.2
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    • pp.85-88
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    • 2008
  • We have investigated the effects of cottonseed extract on the proliferation, differentiation and lipopolysaccharide (LPS)-induced production of local factors in murine clonal osteoblastic MC3T3-E1 cells. Ethanol extract of cotton seed ($4{\sim}63{\mu}g/mL$) significantly increased the proliferatin of MC3T3-E1 cells (p<0.05). Moreover, cottonseed extract ($10{\sim}50{\mu}g/mL$) caused a significant elevation of alkaline phosphatase (ALP) activity and collagen content in the cells. Lipopolysaccharide (LPS) is a potent stimulator of bone resorption in inflammatory diseases. We examined the effect of cottonseed extract on the LPS-induced production of tumor necrosis factor a (TNF-$\alpha$) and nitric oxide (NO) in MC3T3-E1 cells. Treatment with cottonseed extract ($10{\sim}50{\mu}g/mL$) decreased the $5{\mu}g/mL$ LPS-induced production of TNF-$\alpha$ and NO in osteoblasts, suggesting that the antiresorptive action of cottonseed extract may be mediated by decrease in these local factors. This study suggests that cottenseed may contribute to antiresorptive action against osteoblastic cells, resulting in a beneficial effect in promoting the function of osteoblastic cells.

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