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Agronomic and Genetic Evaluation on a Dull Mutant Line Derived from the Sodium Azide Treated 'Namil', a Non-Glutinous Japonica Rice (남일벼 돌연변이 유래 중간찰 계통의 작물학적 특성 및 배유특성 지배유전자위 표지)

  • Chun, Jae-Buhm;Jeung, Ji-Ung;Cho, Seong-Woo;Kim, Woo-Jae;Ha, Ki-Young;Kang, Kyung-Ho;Ko, Jae-Kwon;Kim, Hyun-Soon;Kim, Bo-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.448-457
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    • 2015
  • Developing rice lines with various amylose contents is necessary to diverse usages of rice in terms of raw materials for processed food production, and thereby to promote rice consumption in Korea. A rice mutant line, 'Namil(SA)-dull1' was established through sodium azide mutagenesis on 'Namil', a non-glutinous Korean Japonica rice cultivar. Namil(SA)-dull1' had dull endosperm characteristics and the evaluated amylose content was 12.2%. A total of 94 F2 progenies from a cross between 'Namil(SA)-dull1' and 'Milyang23', a non-glutinous Tongil-type rice cultivar, was used for genetic studies on the endosperm amylose content. Association analyses, between marker genotypes of 53 SSR anchor markers and evaluated amylose contents of each 94 F2:3 seeds, initially localized rice chromosome 6 as the harboring place for the modified allele(s) directing low amylose content of 'Namil(SA)-dull1'. By increasing SSR marker density on the putative chromosomal region followed by association analyses, the target region was narrowed down 0.94 Mbp segment, expanding from 28.95 Mbp to 29.89 Mbp, on rice chromosome 6 pseudomolecule. Among the SSR loci, RM7555 explained 84.2% of total variation of amylose contents in the $F_2$ population. Further physical mapping on the target region directing low amylose content of 'Namil(SA)-dull1' would increase the breeding efficiency in developing promising rice cultivars with various endosperm characteristics.

Effect of Supplementary Radiation on Growth of Greenhouse-Grown Kales (온실재배 케일의 생장에 미치는 보광효과)

  • Heo, Jeong-Wook;Kim, Hyeon-Hwan;Lee, Kwang-Jae;Yoon, Jung-Boem;Lee, Joung-Kwan;Huh, Yoon-Sun;Lee, Ki-Yeol
    • Korean Journal of Environmental Agriculture
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    • v.34 no.1
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    • pp.38-45
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    • 2015
  • BACKGROUND: For commercial production of greenhouse crops under shorter day length condition, supplementary radiation has been usually achieved by the artificial light source with higher electric consumption such as high-pressure sodium, metal halide, or incandescent lamps. Light-Emitting Diodes (LEDs) with several characteristics, however, have been considered as a novel light source for plant production. Effects of supplementary lighting provided by the artificial light sources on growth of Kale seedlings during shorter day length were discussed in this experiment. METHODS AND RESULTS: Kale seedlings were grown under greenhouse under the three wave lamps (3 W), sodium lamps (Na), and red LEDs (peak at 630 nm) during six months, and leaf growth was observed at intervals of about 30 days after light exposure for 6 hours per day at sunrise and sunset. Photosynthetic photon flux (PPF) of supplementary red LEDs on the plant canopy was maintained at 0.1 (RL), 0.6 (RM), and $1.2(RH){\mu}mol/m^2/s$ PPF. PPF in 3 W and Na treatments was measured at $12{\mu}mol/m^2/s$. Natural light (NL) was considered as a control. Leaf fresh weight of the seedlings was more than 100% increased under the 3 W, Na and RH treatment compared to natural light considering as a conventional condition. Sugar synthesis in Kale leaves was significantly promoted by the RM or RH treatment. Leaf yield per $3.3m^2$ exposed by red LEDs of $1.2{\mu}mol/m^2/s$ PPF was 9% and 16% greater than in 3W or Na with a higher PPF, respectively. CONCLUSION: Growth of the leafy Kale seedlings were significantly affected by the supplementary radiation provided by three wave lamp, sodium lamp, and red LEDs with different light intensities during the shorter day length under greenhouse conditions. From this study, it was suggested that the leaf growth and secondary metabolism of Kale seedlings can be controlled by supplementary radiation using red LEDs of $1.2{\mu}mol/m^2/s$ PPF as well as three wave or sodium lamps in the experiment.

Visual fatigue in Watching 3 Dimension Television (3D TV 시청에 있어서 시청 피로)

  • Yoon, Jeong Ho;Lee, Ikhan;Kim, Taehyun;Kim, Jae-Do
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.1
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    • pp.47-52
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    • 2012
  • Purpose: This study was to evaluate visual fatigue with passing of watching 3D TV in short term and with experience of watching 3DTV in long term. Methods: 98 adult subjects aged $33.5{\pm}5.5$ years (22 to 51 years; 12 females and 86 males) agreed to participate in this study. Subjects were asked to watch 52 inch LED 2D and 3D television (Shutter glasses method) at 2.7 meters for 65minutes with wearing their habitual glasses or contact lenses. For evaluating visual fatigue, subjects were verbally responded to 11 questions : eye straining, eye paining, dry eye, sore eye, watery eye, photophobia, blur vision, diplopia, eye fatigue, headache, and dizziness with scale 0 to 3 at each measurement while watching 3D and 2D TV. Results: The mean scores of visual fatigue were $2.08{\pm}2.14$, $3.19{\pm}3.02$, $3.40{\pm}3.37$, $3.53{\pm}3.07 $for after 5 minutes, 25 minutes, 45 minutes, and 65 minutes respectively for 3D TV, and $0.40{\pm}1.03$, $0.22{\pm}0.70$, $0.22{\pm}0.58$, and $0.17{\pm}0.52$ after 25, 45, and 65 minutes respectively for 2D TV. Visual fatigue for watching 3D TV was significantly higher than for watching 2D TV at all measurements sessions (paired t-test, p < 0.001). The visual fatigue significantly increased during watching 3D TV for 65 minutes (p < 0.001, RM-ANOVA). The visual fatigue during watching 3D TV was significantly increased until 25 minutes (paired t-test, p < 0.001), stable after that. For correlation between visual fatigue and 3D watching experience, the more 3D watching experiences were significantly the less visual fatigues in photophobia, blur vision, diplopic and dizzy symptoms (ANOVA, all F(1, 96) = 4.500, all p < 0.05), but there was not significantly different in the other symptoms (ANOVA, F (1, 96) = 2.123, p = 0.148). Conclusions: Visual fatigue for watching 3D TV was higher than for watching 2D TV, increase by 25 minutes. It was different by symptoms for correlation between visual fatigue and 3D watching experience.

Gomphina (Macridiscus) veneriformis (Lamark, 1818) (Bivalvia: Veneridae) in the East Sea of Korea

  • Kim, Yong Ho;Kim, Sung Han;Chung, Ee-Yung;Lee, Chang-Hoon;Kwak, Cheol Woo
    • The Korean Journal of Malacology
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    • v.29 no.4
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    • pp.313-323
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    • 2013
  • Gonad development, the reproductive cycle, first sexual maturty and size at 50% of group sexual maturity (the biological minimum size) of Gomphina (Macridiscus) veneriformis were investigated for clams collected from the coastal waters of Donghae City, the East Sea of Korea by histological, and morphometric analysis. Monthly variations of the gonad index showed a pattern similar to that of the reproductive cycle. The reproductive cycle with the gonad developmental stages in female and male G. (M.) veneriformis can be classified into five successive stages: early active stage (December to March), late active stage (March to June), ripe stage (June to July), partially spawned stage (June to August), and spent / inactive stage (September to December). The spawning period continued from June to August, with a peak between July and August when the seawater temperature exceeds $20^{\circ}C$. The percentages of first sexual maturities of female and male clams ranging from 25.1 to 30.0 mm were 56.3% in females and 61.1% in males, and for clams over 30.1 mm shell length, it was 100%. Shell lengths at 50% of group sexual maturity (biological minimum size, $RM_{50}$) were 27.71 mm in females and 26.31 mm in males. Because harvesting clams < 26.31 mm in shell length could potentially cause a drastic reduction in recruitment, a measure indicating a prohibitory fishing size should be taken for adequate fisheries management.

Development Success of the Secondary Mirror Prototype for Giant Magellan Telescope

  • Koh, Ju Heon;Kim, Young-Soo;Jung, Ho June;Jung, Hwa Kyoung;Yang, Ho-Soon;Kim, Ho-Sang;Lee, Kyoung-Don;Cho, Myung;Park, Won Hyun;Ahn, Hyo-Sung;Hwang, Narae;Yuk, In-Soo;Park, Byeong-Gon
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.85.1-85.1
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    • 2013
  • GMT(Giant Magellan Telescope)는 25.4m의 주경과 3.2m의 부경으로 이루어진 거대망원경이다. 3.2m의 부경은 빠른 tip-tilt로 망원경을 제어하는 Fast Steering Mirror(FSM)와 적응광학계로 이루어진 Adaptive Secondary Mirror (ASM)로 구분된다. 한국천문연구원은 국내외 협력기관들과 함께 부경 FSM의 시험모델개발을 수행하고 있다. 부경은 1.06m의 반사경 일곱 개로 이루어져 있는데, 한 개의 광축 반사경 주위로 여섯개의 비축 반사경이 둘러싸여서 하나의 3.2m 반사경과 같은 기능을 하고 있다. 비축 비구면 반사경에 대한 시험모델의 반사면을 가공한 결과 표면가공 정밀도가 11.7nm rms의 정밀도를 갖는 반사경을 가공하였다(target : <20nm rms). 또한 test-bed를 제작하여 tip-tilt 정밀도를 제어한 결과 성능요구 조건 값인 0.03arcsec 제어에 성공하였고, 더 나아가 0.01arcsec의 tip-tilt 제어도 가능한 것으로 판단된다.

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Effects of Bojeongjeongcheon-tang on Cytokines and Immunoglobulin E in B Cells (보정정천탕의 Cytokine 및 IgE에 대한 조절효과)

  • 권혁성;정주호;김성훈;정승기
    • The Journal of Korean Medicine
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    • v.25 no.2
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    • pp.51-66
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    • 2004
  • Objectives : To evaluate experimentally the clinical effect of Bojeongjeongcheon-tang, we observed the cytokines ($IL-1{\beta}$/TEX>, IL-4, IL-5, IL-6, IL-10, TNF-{\alpha},{\;}TGF-{\beta},{\;}IFN-{\gamma}$) and what effect they have on IgE in B cells of a rat. Methods : First of all, we extracted the spleens of healthy Balb/c mice and separated B cells from them. These B cells were cultured with anti-CD40 mAb (500 ng/ml), rmIL-4 (500 U/ml), Bojeongjeongcheon-tang (100 ug/ml, 10 ug/ml, 1 ug/ml). We used rmiL-10 (50 ng/ml) as a control group. Furthermore, we analyzed the expression of IgE, CD23, CD69 and the coherence of HRF in B cells using a flow cytometer. We also analyzed the cytokine gene expression in B cells by reverse transcriptase-PCR. We also measured B cells proliferation using the Liquid Scintillation Counter. Results : In this study, the Bojeongjeongcheon-tang treated group showed a tendency to decrease depending on the density compared with the control group in the expression of IgE+, CD23+, CD69, HRF. All of the Bojeongjeongcheon-tang treated group showed inhibitory effects with $IL-1{\beta}$, IL-4, IL-5 and proliferating effects with IL-6, IL-10, and $IFN-{\gamma}$ on cytokines transcript expression depending on the density. Meanwhile, $TNF-{\alpha}$ increased in all density. In IgE production, there was inhibitory effect on Bojeongjeongcheon-tang (both 100 ug/ml and 10 ug/ml) of significance (p < 0.01, p < 0.05). Also in B cell proliferation, the result revealed an inhibitory effect of Bojeongjeongcheon-tang (both 100 ug/ml and 10 ug/ml), of significance (p < 0.001, p < 0.01). Conclusions : This study shows that Bojeongjeongcheon-tang has an inhibitory effect on the production and activity of B cells. Also it inhibited CD23, IL-4 activity and IgE production and activation. It is obvious that Bojeongjeongcheon-tang treats asthma by inhibiting the production of histamine and HRF, IL-5 and proliferating IL-10. Also Bojeongjeongcheon-tang has some preventive effects on bronchial change by inhibiting $TGF-{\beta}$, which stimulates the bronchial transformation.

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Biomechanical Validation about Dumbbell Curl Exercise Effects of Virtual Environment (가상환경 변화에 따른 덤벨 컬 운동효과에 관한 운동역학적 검증)

  • Hong, Ah Reum;Kim, Jai Jung;So, Jae Moo
    • Korean Journal of Applied Biomechanics
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    • v.30 no.1
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    • pp.111-119
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    • 2020
  • Objective: The purpose of this study is to apply exercise learning effect to various subjects through training effect and information accumulation based on verification of the effect on dumbbell curl exercises applied with virtual reality. Method: To analyze the effect on the dumbbell curl exercise in the virtual environment, a total of 20 persons with 10 males and 10 females who does not have orthopedics diseases were selected. The dumbbell weight of the subjects was set to a weight of 70% strength of 1RM. At this time, the virtual environment situation was set to four types; presence/absence of virtual environment, preferred colors, and unfavorable colors to perform dumbbell curl exercise. The anaysis of muscle activity was conducted by adhering four surface electrodes (Biceps Brachii, Triceps Brachii, Brachioradialis Muscle, Extensor Carpi Radialis Longus Muscle) on the right upper limbs. Independent sample t-test using SPSS (24.0) program was carried out to analyze average values and standard deviations for each variable depending on the presence/absence of virtual environments and changes in color (preferred colors, unfavorable colors) and the level of significance was set to a=.05. Results: In the eccentric contraction, males showed high muscle activity in the Biceps Brachii under virtual reality. On the other hand, females had high muscle activity in the Biceps Brachii in the absence of virtual reality. Also, in case of a change of colors in the virtual environment, females had the high muscle activity in the unfavorable color in the eccentric contraction. Conclusion: During the dumbbell curl exercise, results of different exercises present depending on gender. When males put VR on and performs a basic dumbbell curl exercise, the effect of Biceps presents Brachii for them while exercising in unfavorable colors. However, since it is the basic research data of muscle exercise using virtual reality, it is necessary to verify whether or not it is effective for myopachynsis through long-term training rather than unity.

Mapping QTL for ratooning ability in advanced backcross lines from an Oryza sativa × O. rufipogon cross

  • Ji, Shi-Dong;Luo, Xiao;Ahn, Sang-Nag
    • Korean Journal of Agricultural Science
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    • v.41 no.1
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    • pp.1-7
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    • 2014
  • Ratooning ability is one of the major different traits from perennial to cultivated rice and annual type. We developed a set of 126 introgression lines derived from a cross between Hwayeong and W1944 (O. rufipogon) to gain an insight into the genetic factors underlying differences between common wild rice and cultivated rice. One IL, CR6 among the 126 ILs of $BC_3F_4$ showed a significant difference in rationing ability compared with Hwayeong. To further characterize the rationing ability, CR6 was selected and crossed to Hwayeong to produce three secondary populations, $BC_4F_2$, $BC_4F_3$ and $BC_5F_2$. In the Hwayeong background, the W1944 allele was associated with an increase in rationing ability. QTL analysis showed that the qRAT5 for rationing ability was linked to RM194 ($R^2$=6.6%, 19.6%, and 44.5% in the $BC_4F_2$, $BC_5F_2$, and $BC_5F_3$, respectively). The putative qRAT5 was also tightly linked to QTLs for spikelets per panicle and grain weight indicating that this region harbors a QTL cluster related to domestication. To our knowledge, this is the first report to map the major QTL for ratooning ability in rice. The SSR markers linked to qRAT5 would be useful in marker-assisted selection for breeding lines with enhanced ratooning ability.

A Comparison of Muscle Activity and Fatigue Between Maximal and Submaximal Repetitions Exercise During the Bench Press (최대 및 최대하 벤치프레스 반복운동 중에 근활성도 및 근피로도의 비교)

  • Seo, Sang-Won;Lee, Ho-Seong
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.65 no.4
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    • pp.678-683
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    • 2016
  • The study aim was to investigate a comparison of muscle activity fatigue between maximal and submaximal strength gains during the bench press in men. It was hypothesized that maximal voluntary contraction (%MVC) would similar gains between maximal and submaximal strength gains during the bench press, but median frequency (MDF) would increase in maximal compared with submaximal exercise. Employing a randomized, counterbalanced crossover design, 12 men were asked to perform maximal repetitions and submaximal repetitions (concentric: 1-s, eccentric: 1-s, 2-s/repetition) to failure with a load of 85% of 1RM for the bench press, with a 3-minute recovery between the sets. Each subject was tested for the number of repetitions and sets, total work in bench press. Surface electromyography (EMG) was recorded from the pectoralis major, deltoid anterior, and triceps brachii for %MVC and MDF. Total work was significantly higher in the submaximal repetition exercise than that the maximal repetition exercise (p<.05). Muscle fatigue of pectoralis major, deltoid anterior and triceps brachii were significantly smaller in the submaximnal repetition exercise than that the maximal repetition exercise (p<.05, respectively). However, muscle activity of pectoralis major, deltoid anterior, and triceps brachii were not significantly different between exercises. Our study showed that a smaller muscle fatigue in submaximal repetitions, despite higher in total work and a similar in muscle activity were observed. These results suggest that submaximal repetitions during the bench press would be enhanced intensity and/or volume compared with maximal repetitions exercise.

Antiplasmodial and Cytotoxic Activities of Toad Venoms from Southern Amazon, Brazil

  • Banfi, Felipe Finger;Guedes, Karla de Sena;Andrighetti, Carla Regina;Aguiar, Ana Carolina;Debiasi, Bryan Wender;Noronha, Janaina da Costa;Rodrigues, Domingos de Jesus;Vieira, Gerardo Magela Junior;Sanchez, Bruno Antonio Marinho
    • Parasites, Hosts and Diseases
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    • v.54 no.4
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    • pp.415-421
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    • 2016
  • The drug-resistance of malaria parasites is the main problem in the disease control. The huge Brazilian biodiversity promotes the search for new compounds, where the animal kingdom is proving to be a promising source of bioactive compounds. The main objective of this study was to evaluate the antiplasmodial and cytotoxic activity of the compounds obtained from the toad venoms of Brazilian Amazon. Toad venoms were collected from the secretion of Rhinella marina and Rhaebo guttatus in Mato Grosso State, Brazil. The powder was extracted at room temperature, yielding 2 extracts (RG and RM) and a substance ('1') identified as a bufadienolide, named telocinobufagin. Growth inhibition, intraerythrocytic development, and parasite morphology were evaluated in culture by microscopic observations of Giemsa-stained thin blood films. Cytotoxicity was determined against HepG2 and BGM cells by MTT and neutral red assays. The 2 extracts and the pure substance ('1') tested were active against chloroquine-resistant Plasmodium falciparum strain, demonstrating lower $IC_{50}$ values. In cytotoxic tests, the 2 extracts and substance '1' showed pronounced lethal effects on chloroquine-resistant P. faciparum strain and low cytotoxic effect, highlighting toad parotoid gland secretions as a promising source of novel lead antiplasmodial compounds.