• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1054-1059
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• 2005

An extracellular protease was identified from Bacillus subtilis KCCM 10257 by N-terminal sequencing and mass spectral analysis. The molecular mass of the extracellular protease was estimated to be 28 kDa by SDS-PAGE. Sequencing of the N-terminal of the protease revealed the sequence of A(G,S,R)QXVPYG(A)V(P,L)SQ. The N-terminal sequence exhibited close similarity to the sequence of other proteases from Bacillus sp. A mass list of the monoisotopic peaks in the MALDI-TOF spectrum was searched after peptide fragmentation of the protease. Six peptide sequences exhibiting monoisotopic masses of 1,276.61, 1,513.67, 1,652.81, 1,661.83, 1,252.61, and 1,033.46 were observed from the fragmented protease. These monisotopic masses corresponded to the lytic enzyme L27 from Bacillus subtilis 168, and the Mowse score was found to be 75. A doubly charged Top product (MS) at a m/z of 517.3 exhibiting a molecular mass of 1034.6 was further analyzed by de novo sequencing using a PE Sciex QSTAR Hybrid Quadropole-TOF (MS/MS) mass spectrometer. MS/MS spectra of the Top product (MS) at a m/z of 517.3 obtained from the fragmented peptide mixture of protease with Q-star contained the b-ion series of 114.2, 171.2, 286.2, 357.2, 504.2, 667.4, 830.1, and 887.1 and y-ion series of 147.5, 204.2, 367.2, 530.3, 677.4, 748.4, 863.4, and 920.5. The sequence of analyzed peptide ion was identified as LGDAFYYG from the b- and y-ion series by de novo sequencing and corresponded to the results from the MALDI-TOF spectrum. From these results the extracellular protease from Bacillus subtilis KCCM 10257 was successfully identified with the lytic enzyme L27 from Bacillus subtilis 168.

• Journal of Plant Biotechnology
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• 제35권3호
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• pp.203-208
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• 2008

Philodendron cannifolium을 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실험을 실시하였다. Philodendron의 경정에서 부정 다아체 형성은 BA $2.0{\sim}5.0$ mg/L 첨가배지와 TDZ $0.05{\sim}1.0$ mg/L 첨가배지에서 양호하였다. 그러나 TDZ이 첨가된 배지에서 생육한 식물체는 모두 엽록소가 결핍되었다. 신초경정에서 형성된 부정 다아체를 $5{\sim}7$ mm 정도로 종으로 절단하여 배양한 결과 BA $1.0{\sim}3.0$ mg/L가 첨가된 배지에서 신초수가 30개 이상으로 신초의 증식이 양호하였다. 그러나 BA가 고농도(10.0 mg/L)로 첨가된 배지 및 TDZ이 첨가된 배지는 기부의 callus가 이상 비대하였다. 증식된 신초의 색도 cream색으로 엽록소가 부족하였다. 증식된 신초의 생육 및 발근은 IBA $0.5{\sim}2.0$ mg/L가 첨가된 MS 배지에서 양호하였다. 발근된 신초의 순화는 vermiculite와 perlite 및 vermiculite의 1:1 혼합용토가 적합하였다. MS배지에 활성탄 10 g/L와 sucrose 30 g/L가 첨가된 액체배지 15 mL를 첨가하여 신초를 생육시킨 다음, IBA $500{\sim}2,000$ ppm이 첨가된 용액에서 10 초간 침지하여 perlite와 vermiculite가 1:1로 혼합된 용토에 재식하였을 때, 신초의 생육 및 발근이 양호하였다.

• 한국전기전자재료학회논문지
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• 제30권9호
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• pp.583-588
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• 2017

Thermal batteries are used in military power sources that require robustness and long storage life for applications in missiles and torpedoes. $FeS_2$ powder is currently used as a cathode material because of its high specific energy density, environmental non-toxicity, and low cost. $MS_2$ (M = Fe, Ni, Co) cathodes have been explored as novel candidates for thermal batteries in many studies; however, the discharge characteristics (1, 2, 3 plateau) of single cells in thermal batteries with different cathodes have not been elucidated in detail. In this study, we independently analyzed the discharge voltage and calculated the total polarizations of single cells using $MS_2$ cathodes. Based on the results of this study, we propose $NiS_2$ as a potential cathode material for use in thermal batteries.

• 대한치매학회지
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• 제17권4호
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• pp.131-136
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• 2018

Alzheimer's disease (AD) related genes have been elucidated by advanced genetic techniques. Familial autosomal dominant AD genes founded by linkage analyses are APP, PSEN1, PSEN2, ABCA7, and SORL1. Genome-wide association studies have found risk genes such as ABCA7, BIN1, CASS4, CD33, CD2AP, CELF1, CLU, CR1, DSG2, EPHA1, FERMT2, HLA-DRB5-HLA-DRB1, INPP5D, MEF2C, MS4A6A/MS4A4E, NME8, PICALM, PTK2B, SLC24A4, SORL1, and ZCWPW1. ABCA7, SORL1, TREM2, and APOE are proved to have high odds ratio (>2) in risk of AD using next generation sequencing studies. Thanks to the promising genetic techniques such as CRISPR-CAS9 and single-cell RNA sequencing opened a new era in genetics. CRISPR-CAS9 can directly link genetic knowledge to future treatment. Single-cell RNA sequencing are providing useful information on cell biology and pathogenesis of diverse diseases.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 춘계학술대회
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• pp.1020-1022
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• 2013

본 논문에서는 펄스방사선 선량 측정을 위한 Peak Detector를 설계하였다. 펄스방사선은 수ns ~ 수십ns의 초고속의 신호로 Peak 값을 측정하기 어렵다. Peak Detector는 센서에서 발생한 Peak 전압을 수ms 동안 유지하므로 ADC를 이용하여 데이터를 쉽게 측정할 수 있다. Peak Detector 시뮬레이션 결과 Peak 값을 1ms 이상 유지하는 것을 확인하였으며 펄스방사선 조사시험 결과 $1.95{\times}10^6rad/s$의 선량을 확인하였다.

• 전기학회논문지
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• 제59권2호
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• pp.355-358
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• 2010

As CMOS technology continues to scale down, signal processing is favorably done in the digital domain, which requires Analog-to-Digital (A/D) Converter to be integrated on-chip. This paper presents a design methodology of 12-bit 1-MS/s Rail-to-Rail fully differential SAR ADC using Deep N-well Switch based on binary search algorithm. Proposed A/D Converter has the following architecture and techniques. Firstly, chip size and power consumption is reduced due to split capacitor array architecture and charge recycling method. Secondly, fully differential architecture is used to reduce noise between the digital part and converters. Finally, to reduce the mismatch effect and noise error, the circuit is designed to be available for Rail-to-Rail input range using simple Deep N-well switch. The A/D Converter fabricated in a TSMC 0.18um 1P6M CMOS technology and has a Signal-to-Noise-and-Distortion-Ratio(SNDR) of 69 dB and Free-Dynamic-Range (SFDR) of 73 dB. The occupied active area is $0.6mm^2$.

• 가정과삶의질연구
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• 제28권5호
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• pp.167-181
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• 2010

This research was designed to explore the meaning of housing among Korean Chinese in Harbin, China. In particular, the meaning of housing was examined by using the pathway approach. Utilizing qualitative research methods, this study administered the in-depth interview on the oral history of an individual life, and the 5 elderly persons in their 60s and 70s participated in the individualized interviews that were conducted from May 28 to 31 in 2010. The main findings of meaning of housing were as follows; 1. Similarly to the meaning of housing in 1970s and 1980s in Korea, house was viewed as both a shelter for family members and relatives and a place for their comfort. 2. Prior to multi-story residences, Harbin had only 3 different forms of single-story houses available; Chinese style with Kang and soil room(地室), Korean style with 'Ondol', and Russian style with open floor and Pechka, The promotion at work enabled participants to move to multi-story residences, their moving time varied from 1970 to 1991, and the residential moving determined their current housing status. 3. Multi-story residences were available around 1970s, floor-heating system was introduced from 1990s, and high-rise apartments were built from 1998. Korean Chinese(朝鮮族) weren't satisfied with the spatial composition of individual units embedded into the Chinese culture, especially, entrance, kitchen, bathroom and veranda. 4. Based on assimilation through socialism, adaptation to socialist society and capitalist acculturation, the lifestyles of the interviewees were categorized into five types - capitalist-proactive(Ms. KS), socialist-pragmatic(Ms. J), socialist-inducive(Ms. KY), family centered-conservative(Ms. L), and socialist-adaptive(Ms. P). This study implies that housing-related services for Korean Chinese are necessarily provided so as to embrace their life style and cultural identity in housing design, and further studies need to be explored.

• 분석과학
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• 제17권2호
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• pp.130-144
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• 2004

최근 A, B 공단지역 및 주변 지역의 대기오염문제가 중요한 이슈가 됨에 따라 이 지역의 대기 중 VOCs를 측정하였다. 시료는 air sampler를 사용하여 Carbotrap에 흡착시킨 후 열탈착시스템을 사용하여 탈착시켜 가스크로마토그래피/질량분석법(GC/MS)으로 분석하였다. 모든 휘발성유기화합물들은 좋은 크로마토그래프 성질을 보였고 GC/MS-SIM에서 높은 감도를 보였다. 검출한계는 0.01-0.1 ppt(v/v)를 검량선의 직선성은 0.995이상을 보였다. 분석 결과 benzene, toluene, ethylbenzene, xylene, n-hexane, fluorotrichloromethane, carbon tetrachloride, 1,2-dichloroethane, 1,1,1-trichloroethane, trichloroethylene, tetrachloroethylene, styrene, 1,3,5-trimethylbenzene, 1,2,4-trimethylbenzene, sec-butylbenzene, naphthalene 등 16개의 VOCs가 검출되었으며, 평균 농도로서 0.81, 5.02 1.30, 3.0, 0.81, 37.9, 0.07, 0.15, 0.15, 0.79, 0.06, 0.33, 0.03, 0.12, 0.23 그리고 0.35 ppb(v/v)으로 나타났다. 전반적으로 총 VOCs의 농도는 여름철보다 가을 및 겨울철에 더 높게 검출되었으나 WHO의 기준치를 초과한 경우는 없었다.

• 전자공학회논문지C
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• 제34C권11호
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• pp.66-75
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• 1997

We derive the system design parameters to implement the receiving system for the PCS mobile station to satisfy the J-sTD-018 which is the PCS mobile station(MS) minimum performance. Also we analyze the system performance and intermodulation spurious due to the values of a device cause the system performance degradation, is proposed. The simulation shows the receiver's maximum system noise figure to satisfy the receiver selectivity is approximately 11 dB. While the MS noise figure is 10dB with system margin 1 dB, the minimum selectivity is -71 dB at 1.25MHz frequency offset from the carrier frequency. And the input 3rd order intercept point of the MS class I and the MS class II~V is -9.5 dBm and -14dBm respectively. When the interference power level at the receiver is small, the receiver has better performance as we increase the gain of the LNA. However, when the interference level at the receiver is large, the receiver performance is heavily affected by the spurious as we increase the gain of the LNA. Thus, we proved the effectiveness of the LNA On/Off switching technique as to reduce the effect of the spurious.

• 대한화학회지
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• 제54권1호
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• pp.59-64
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• 2010

일련의 불소화된 티아디아졸 3, 트리아졸 4, 그리고 옥사디아졸 5이 (2-(6-Methyl-2-P-tolyl-lH-imidazo[1,2-a]pyridin-3-yl)핵종을 가지고 있는 티오세미카르바지드로 부터 합성되어진다. 초음파조사 방법 뿐만 아니라 일반적인 방법에 의해 반응이 진행되었다. 모든 생성물들은 IR, 1H NMR, MS로 구조가 결정되었고, 이들 화합물의 항균활성을 스크닝하였다.