• 환경영향평가
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• 제28권5호
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• pp.457-470
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• 2019

서남해 해상풍력 실증단지 해역을 중심으로 구축된 모니터링 자료를 분석하여 해양물리, 해양수질, 해저퇴적물의 환경 특성을 파악하고 현황을 제시하였다. 파고와 부유사 농도, 유속과 부유사 농도의 상관관계를 분석하여 연구 해역 토사의 부유 특성을 고찰하였다. 춘계 시(1 개월) 파고와 부유사 농도 사이에는 상관계수 0.61~0.69의 상관성을 보였으며, 1 m 이상의 높은 파고가 발생하면 75 mg/L 이상의 부유사 농도가 관측되었다. 수질평가지수 산정 시 필요한 항목을 중심으로 수질 현황을 분석하였으며 대부분의 정점에서 1등급(매우 좋음)을 보이고 있었다. 클로로필-a의 변동폭이 다른 항목에 비해 상대적으로 높게 나타나 수질평가지수에 영향을 주는 중요 인자로 파악되었다. 해저퇴적물 내 중금속 현황은 모든 항목의 평균값이 해양환경 주의기준 이하로 검출되었으며 강열감량과 입도분포는 높은 연관성을 보였다.

• 한국미생물·생명공학회지
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• 제6권1호
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• pp.41-46
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• 1978

진균류인 Aspergilus flavus의 대사생성물인 aflatoxin이 발암성물질이카는 것이 알려진 이래 비병원성 미생물이라고 ale어왔던 여러가지 미생물의 독성물질 생성여부에 관한 연구가 많이 이루어져 우리 식생활에 미생물독 즉 mycotoxin에 대한 문제는 더욱 중요하게 되였다. 본 실험에서는 토양에서 분리한 방선균속 균주가 생성하는 살어성물질의 기본적인 성질은 pH 3～7사이는 안정하였으나 PH 8이상에서는 매우 불안정하였으며 열에 대해서도 비교적 불안정한 물질이었으며 어종과 어체중에 따른 독성의 비교는 체중이 증가함에 따라 생존시간이 길었지만 버들붕어와 미꾸라지가 타어종에 비해서 본 독성물질에 대한 저항성이 매우 켰다. 일반적으로 미생물독의 생물에 대한 작용$^{(24~26)}$은 hemolytic necrotizing, proteolytic, neurotoxic, cardiotoxic, hepatotoxic, nephrotoxic 등을 들 수 있으나 대부분의 독성물질은 상기 독작용 가운데 한 두 가지로서 그 주인이 된다. 본 물질의 잉어에 대한 각 조직의 succinic dehydrogenase 활성에 대한 조해작용을 조사한 결과 근육, 심장, 뇌 조직에 강한 조해현상을 나타내었다. 본 독성물질생성에 대한 금속 ion의 영향을 조사한 결과 $Cu^{2＋}$, $Pb^{2＋}$의 첨가가 크게 관계한다는 것을 알았으며 기타 본물질성장에 carbohydrate, amino acid, vitamin이 미치는 영향을 조사해 보고자 하며 어류이외의 타 온혈동물의 조직에 본독성물질을 작용시켜 그 결과를 알고자 한다. 한편 본 물질이 잉어의 각 조직에 대한 succinic dehydrogenase 활성에 미치는 정도 및 본 물질생성에 미치는 금속염의 영향을 볼 때 송$^{19~22}$ 등이 분이한 물질과는 차이가 있음을 알았다.소되었다. Alkaline protease는 배양 24시간에서 48시간까지는 0.1%구와 0.5%구가 대조구보다 효소력이 높았고 그 이상의 인삼엑기스가 첨가된 처리구보다 효소력이 낮았다. 그러나 48시간 이후부터는 효소력은 인삼엑기스 첨가량 순으로 감소를 나타내었다. period of May 13th-June 4th 1976. The study data was collected by the direct interview method based on the questionnaire which the investigator made out. The study result was analyzed by percentage, t - test. The findings can be summarized as follows: 1. General characteristics of nurses doing duty on puerperal ward: a. Nurses′average age was 24.8 years old. b. 84.6% had educational background of 4 years of college. c. 69.2% had a religion. d. 53.8% were married. e. 53.8% had clinical experience of 1 year -3 years. f, 61.5% did duty on puerperal ward during 1 year -3 years. g. 46.2% desired to do duty on obstetric ? gynecologic ward. 2. General characteristics of the women who were studied during their puerperal period: a. Women′s average age was 26.4 years old. b. 79.5% had educational background above high school.

• Korean Journal of Radiology
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• 제21권7호
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• pp.880-890
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• 2020

Objective: Patients with chronic obstructive pulmonary disease (COPD) are known to be at risk of osteoporosis. The purpose of this study was to evaluate the association between thoracic vertebral bone density measured on chest CT (D_Thorax) and clinical variables, including survival, in patients with COPD. Materials and Methods: A total of 322 patients with COPD were selected from the Korean Obstructive Lung Disease (KOLD) cohort. D_Thorax was measured by averaging the CT values of three consecutive vertebral bodies at the level of the left main coronary artery with a round region of interest as large as possible within the anterior column of each vertebral body using an in-house software. Associations between D_Thorax and clinical variables, including survival, pulmonary function test (PFT) results, and CT densitometry, were evaluated. Results: The median follow-up time was 7.3 years (range: 0.1-12.4 years). Fifty-six patients (17.4%) died. D_Thorax differed significantly between the different Global Initiative for Chronic Obstructive Lung Disease stages. D_Thorax correlated positively with body mass index (BMI), some PFT results, and the six-minute walk distance, and correlated negatively with the emphysema index (EI) (all p < 0.05). In the univariate Cox analysis, older age (hazard ratio [HR], 3.617; 95% confidence interval [CI], 2.119-6.173, p < 0.001), lower BMI (HR, 3.589; 95% CI, 2.122-6.071, p < 0.001), lower forced expiratory volume in one second (FEV₁) (HR, 2.975; 95% CI, 1.682-5.262, p < 0.001), lower diffusing capacity of the lung for carbon monoxide corrected with hemoglobin (DL_CO) (HR, 4.595; 95% CI, 2.665-7.924, p < 0.001), higher EI (HR, 3.722; 95% CI, 2.192-6.319, p < 0.001), presence of vertebral fractures (HR, 2.062; 95% CI, 1.154-3.683, p = 0.015), and lower D_Thorax (HR, 2.773; 95% CI, 1.620-4.746, p < 0.001) were significantly associated with all-cause mortality and lung-related mortality. In the multivariate Cox analysis, lower D_Thorax (HR, 1.957; 95% CI, 1.075-3.563, p = 0.028) along with older age, lower BMI, lower FEV₁, and lower DL_CO were independent predictors of all-cause mortality. Conclusion: The thoracic vertebral bone density measured on chest CT demonstrated significant associations with the patients' mortality and clinical variables of disease severity in the COPD patients included in KOLD cohort.

• Biomolecules & Therapeutics
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• 제6권3호
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• pp.289-295
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• 1998

The bioequivalence of two nilvadipine products was evaluated in 16 normal male volunteers (age 22-32 yr, body weight 57-80 kg) following sidle oral dose. Test product was Overca $l_{R}$ tablet (Choong-Wae Pharm. Corp., Korea) and reference product was Nivadi $l_{R}$ tablet (Hyundai Pharm. Corp., Korea). Both products contain 4 mg of nilvadipine. One tablet of the test or the reference product was administered to the volunteers, respectively, by randomized two period cross-over study (2$\times$2 Latin square method). The determination of nilvadipine was accomplished using a validated capillary column GC with electron-capture detection. As a result of the assay validation, the quantiflcation of nilvadipine in human plasma by this technique was possible down to 0.5 ng/ml using 1 ml of plasma. Absolute overall recovery from five replicate analyses of nilvadipine-spiked sample were 88.4$\pm$ 10.24% (mean$\pm$ 5.D.) for human plasma of 10 ng/ml. The coefficients of variation (C.V.) were less than 20% and the actual concentration of nilvadipine measured by GC ranged from 80 to 99% in all plasma. Average drug concentrations at each sampling time and pharmacokinetic parameters calculated were not significantly different between two products (p>0.05); the area under the curve from time zero to 8 hr (AUCo-$_{8 hr}$) (22.8$\pm$5.90 vs 22.2$\pm$6.10 ng . hr/ml), maximum plasma concentration ( $C_{max}$) (10.0$\pm$2.85 vs 9.3$\pm$3.28 ng/ml) and time to reach maximum plasma concentration ( $T_{max}$) (1.2$\pm$0.31 vs 1.3 $\pm$0.47 hr). The differences of mean AU $Co_{8hr}$ $C_{max}$, and $T_{max}$ between the two products (2.25, 7.65, and 10.30%, respectively) were less than 20%. The power (1-$\beta$) and treaeent difference (7) for AU $Co_{8hr}$, and $C_{max}$ were more than 0.8 and less than 0.2, respectively. Although the power for Tmax was under 0.8, Tm\ulcorner of the two products was not significantly different from each other (p>0. 05). These results suggest that the bioavailability of Overeat tablet is not significantly different from that of Nivadil tablet. Therefore, two products are bioequivalent based on the current results.sults.lts.lts.lts.

• Natural Product Sciences
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• 제17권2호
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• pp.147-159
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• 2011

A high-performance liquid chromatography-electrospray ionization-tandem mass spectrometric method was developed to determine simultaneously eight marker constituents of Forsythiae fructus, and subsequently applied it to classify its two botanical origins. The marker compounds of Forsythia suspensa were phillyrin, pinoresinol, phillygenin, lariciresinol and forsythiaside; those of F.viridissima were arctiin, arctigenin and matairesinol. Separation of the eight analytes was achieved on a phenyl-hexyl column (150${\times}$2.0 mm i.d., 3 ${\mu}M$) using gradient elution with the mobile phase: (A) 10% acetonitrile in 0.5% acetic acid, (B) 40% aqueous acetonitrile. A few fragment ions specific to the types of lignans, among the product ions generated by collisonally induced dissociation (CID) of molecular ion clusters, such as [M-H]$^-$ or [M+OAc]$^-$ were used not only for fingerprinting analysis but for the quantification of each epimer by using multiple-reaction monitoring mode. It was shown good linearity ($r^2{\geq}$ 0.9998) over the wide range of all analytes; intra- and inter-day precisions (RSD, %) were within 9.14% and the accuracy ranged from 84.3 to 115.1%. The analytical results of 40 drug samples, combined with multivariate statistical analyses - principal component analysis (PCA) and hierarchical cluster analysis (HCA) - clearly demonstrated the classification of the test samples according to their botanical origins. This method would provide a practical strategy for assessing the authenticity or quality of the herbal drug.

• 한국강구조학회 논문집
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• 제16권5호통권72호
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• pp.661-669
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• 2004

철골구조에 있어서 기둥 이음부의 설계는 단순히 적당한 틈에 용접이나 볼트에 의한 이음으로 해석 및 설계가 이루어지고 있는 것이 보편적인 현실이다. 이때 이음부에서 메탈터치에 의하여 상부의 응력을 하부로 전달하는 정도를 규정하는 기준은 각 나라마다 다르다. 미국은 설계자의 판단에 따라 모두 전달 가능하다는 규정아래 설계가 이루어지고 있으며, 우리나라와 일본의 경우는 25%까지 전달 가능하다는 규준에 의해 설계가 이루어지고 있어 그 차이가 너무 크다. 따라서 본 연구에서는 메탈터치에 의한 이음의 응력체계를 규명하여 이음의 합리성을 증진시키고, 이음의 경제성 및 시공성을 위해 틈의 위치 및 이음방법을 매개변수로 하여 메탈터치 실험을 실시하여 미국 및 일본 규준과 비교하였다. 또한 메탈터치 이음부의 거동을 실험적인 방법과 해석적인 방법을 통하여 분석하여 메탈터치 이음부의 설계에 필요한 기초 자료를 제공하고자 한다.

• 한국동물위생학회지
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• 제28권3호
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• pp.203-213
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• 2005

Oxytetracycline, tetracycline, chlortetracycline and doxycycline in honey were separated by solid phase extraction (SPE) and determined with high performance liquid chromatography (HPLC) with UV/Visible detector. Analysis was carried out using following conditions: XTerra $C_8$ column $(3.9\times150mm\;i.d. 5{\mu}m)$, mobile phase composed of 0.01M oxalic acid : methanol : acetonitrile (820 : 80 : 100, v/v/v), isocratic pump at a flow rate of 0.9 ml/min. and $50{\mu}l$ of injection volume, UV/Visible detector with wavelength of 360nm. The calibration curves of four tetracyclines showed linearity $(\gamma^2>0.999)$ at concentration range of $100\~1,000 ng/ml$. The recoveries in fortified honey represented more than $70\%$ with low coefficient of variation $(<10\%)$ for concentration range of four tetracyclines. The detection limits for oxytetracycline, tetracycline, chlortetracycline and doxycycline were 13.8, 14.6, 26.2 and 24.9ng/g in acacia honey. respectively. We also monitored tetracyclines residue in domestic honey [n : 38, acacia (20), wild flower (18) ] and foreign honey [n=22, legally distributed (13), illegally distributed (9)] using modified Charm II screening and HPLC confirmation methods. Seven of the 60 samples $(11.7\%)$ were suspect positive using modified Charm II screening test. Chlortetracycline residue was found in one foreign honey (illegally distributed) tested at concentrations of 0.22 ppm. Conclusively, for more effective control of tetracyclines used in beekeeping should be further survey for residues in honey and also national guidelines (maximum residue limit : MRL) and methods should be obligatory.

• 자원환경지질
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• 제35권2호
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• pp.149-154
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• 2002

오염된 토양의 복원에서 계면 활성제 용액의 조건에 따른 영향을 조사하기 위하여 주상실험이 실행되었다. 계면 활성제 용액의 조건에는 농도와 pH가 선택되었으며 모델 소수성 유기화합물은 삼염화벤젠, 그리고 DOSL라 OPEE 계면활성제가 본 연구에 사용 되었다. 또한 미국의 아이오와 토양이 본 연구에 이용 되었다. 실험 결과, 계면활성제 용액의 최적 조건은 농도 4%(v/v), pH 10 이며, 이러한 최적 조건이 적용될 때 93-98%의 삼염화벤젠이 오염된 토양으로부터 회수되었다. 규명된 최적 조건은 게면 활성제를 이용하여 삼염화벤젠으로 오염된 토양 복원에 유용하게 사용 될 수 있다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2000년도 Proceedings of 2000 KSAM International Symposium and Spring Meeting
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• pp.53-60
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• 2000

Strain BH5 was isolated from naturally fermented Kimchi and identified as a bacteriocin producer, which has bactericidal activity against Micrococcus flavus ATCC 10240. Strain BH5 was identified tentatively as Lactococcus lactis by the API test and some characteristics. Lactococcus lactis BH5 showed a broad spectrum of activity against most of the non-pathogenic and pathogenic microorganisms tested by the modified deferred method. The activity of lacticin BH5, named tentatively as the bacteriocin produced by Lactococcus lactis BH5, was detected at the mid-log growth phase, reached its maximum during the early stationary phase, and decreased after the late stationary phase. Lacticin BH5 also showed a relatively broad spectrum of activity against non-pathogenic and pathogenic microorganisms as tested by the spot-on-lawn method. Its antimicrobial activity on sensitive indicator cells was completely disappeared by protease XIV or ${\alpha}$-chymotrypsin. The inhibitory activities of lacticin BH5 were detected during treatments up to 100$^{\circ}C$ for 30 min. Lacticin BH5 was very stable over a pH range of 2.0 to 9.0 and was stable with all the organic solvents examined. The cell concentration and bacteriocin production in strain BH5 were maximum when grown at 30$^{\circ}C$ in a modified MRS medium supplemented with 0.5% tryptone, 1.0% yeast extract, and 0.5% beef extract as nitrogen sources. It demonstrated a typical bactericidal mode of inhibition against Micrococcus flavus ATCC 10240. Lacticin BH5 was purified through ammonium sulfate precipitation, ethanol precipitation, and CM-Sepharose column chromatography. The apparent molecular mass of lacticin BH5 was estimated to be in the region of 3.7 kDa, by the direct detection of bactericidal activity after SDS-PAGE. Mutant strain NO141 which was isolated by nitrosoguanidine mutagenesis produced about 4 fold more bacteriocin than the wild type.

• 한국지반공학회논문집
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• 제22권1호
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• pp.35-44
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• 2006

일반적으로 현장지반은 대부분 일정량의 세립분을 포함하고 있으므로 세립분 함유량을 고려한 지반 거동에 관한 연구가 요구된다. 이와 더불어, 기초 지반은 하중초기단계부터 상당한 비선형 거동특성을 나타내고 있어, 응력단계에 따른 지반의 비선형성 또한 매우 중요한 고려사항이다. 본 연구에서는 일련의 실내시험을 수행하여 지반의 전단강도 및 비선형 감쇠 특성을 도출하고자 하였으며, 수정 Hyerbolic 모델을 적용하여 분석된 실트질 모래 지반의 비선형 거동특성은 실트함유량과 상대밀도에 의해 정량화되었다. 실트질 모래 시료의 응력-변형률 곡선을 도출하기 위해 세립분 함유량을 변화시켜가며 일련의 삼축압축시험이 수행되었다. 또한 비선형 특성의 정규화에 요구되는 미소변형률 구간의 초기전단탄성계수의 도출을 위해서 삼축압축시험의 시료조건과 유사한 시료에 대해 공진주시험이 수행되었다. 또한 실트함유량별로 도출된 비선형 특성치는 상대밀도의 영향이 가장 큰 것으로 나타났으며, 상대밀도 증가에 따라 파괴시의 탄성계수의 비인 f값은 감소하고, 지반강성도 감쇠율을 나타내는 9의 경우 증가하는 경향을 나타내었다. 이와 더불어 비선형 특성치를 상대밀도 $D_R$을 간극비로 환산한 절대적 간극비 $e_{sk}$에 따른 정량화 결과의 경우, $e_{sk}$의 증가에 따라 f값은 증가하고 g값은 감소하는 경향을 나타내었다.