• Clinical and Experimental Reproductive Medicine
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• 제17권1호
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• pp.21-28
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• 1990

19-nortestosterone(NORA)와 19-norandrostenedione(NT)는 정소내 aromatization과정중 중간 대사물로 검출된다. 본 연구는 이들을 장기간 투여하여 정소 및 부속기관의 무게, 혈청내 testosterone(T)의 농도, 정자형성에 미치는 영향을 조사하였다. NORA, NT 및 TE를 $300/50{\mu}l$농도로 주당 3회씩 12주간 정소에 직접 주사(intratesticular injection, i.t.)하였다. 또한 GnRH antagonist(RS68439)를 처리하여 혈청내 생식소자극호르몬 (GTH)을 감소시킨후 위 호르몬들을 동일방법으로 처리하여 이들의 보상작용을 조사하였다. NORA는 정소무게를 감소시키지 않았으나 GnRH antagonist를 처리하여 감소된 정소무게를 현저하게 보상하였다(P<0.05). NORA, NT, TE는 모두 부속기관의 무게를 증가시켰으며, RS68439가 감소시킨 부속기관의 무게를 현저히 증가시켰다. 그러나 이들은 부정소(epididymis)에는 영향을 주지않았으며 RS68439처리군에서는 보상작용을 나타내었다. 이들의 처리로 혈청내 LH농도는 완전히 감소하였으나 FSH의 농도에는 영향을 나타내지 않았다. 그리고 혈청내 T의 농도를 증가시켰다. NORA는 정자형성과정중 7단계의 spermatid의 수를 현저히 감소시켰다. 위 결과로 보아 NORA는 GnRH antagonist로 FSH의 분비가 억제된 쥐의 FSH분비를 촉진하며, T의 농도를 증가시켜 정자형성과정을 억제하는 것으로 추론된다.

• Clinical and Experimental Reproductive Medicine
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• 제21권3호
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• pp.305-314
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• 1994

19-norandrostenedione(19-NORA) is known as an intermediate in the metabolic pathway from androstenedione to estrone. Administration of esterified 19-nortestosterone, anabolic steroid, reduces serum gonadotropin and testosterone concentration, and results in reversible azoospermia in men. 19-NORA have been isolated from testis, but its function in testis is not clear yet. Therefore, this study was designed to determine the effect of 19-NORA on steroidogenesis and on spermatogenesis. 19-NORA was administrated by single intratesticular injection to adult male rats weighing 350-400 g in dose of 1 mg/50${\mu}l$. The serum and testis were collected on 1, 3, 7, 12, 48 hr after injection. The histological differences in testis were observed by routine paraffin method. The concentrations of testosterone and estradiol in serum and in left testis were determined by the conventional radioimmunoassays. One hour after 19-NORA treatment, serum concentrations of testosterone and estradiol increased significantly, compared to those of pre-treated(0 hr) group, and reduced gradually to the control level on 7 hour after injection. The concentration of testosterone in left testis increased slightly 1 hour after injection, and estradiol level increased significantly(p<0.05). Also, testosterone and estradiol level of control group revealed no difference with pre-treated (0 hr) group. Gonad index, structure of seminiferous tubules, and the number of step 7 th spermatid were simillar to control group. The present study suggests that the elevation of testosterone level results from increment of estradiol followed by the rapid metabolism of 19-NORA at 7 hour after injection, and then testosterone concentration may be recovered to control level by feedback mechanism of hypothalamus-hypothysis-testis axis.

• 약학회지
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• 제16권3호
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• pp.137-147
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• 1972

Estrone, which is obtainable from 19-hydroxycholesterol acetate through microbial oxidation, was chemically transformed to orally active estrogens, ethynylestradiol and methylestradiol. For progestin synthesis, $5{\alpha}$-bromo-619-oxidoisoandrosterone, was harvested from the culture broth(ATCC:19170), in which $5{\alpha}$-bromo-619-oxidocholestanol, acetate was added as a carbon source. Methylestrenolone, a potent orally active progestin, was synthesized from the harvested product via several reactions. Norethindrone, a popular progestin, was also prepared from 19-norandrostenedione. 19-Norandrostenedione is also available through microbial oxidation and chemical modification of $5{\alpha}$-chlor-619-oxidocholestanol, acetate.