• Clinical and Experimental Pediatrics
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• 제49권11호
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• pp.1125-1139
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• 2006

In 1991, the Ministry of Health & Social affairs adopted a nationwide service program for neonatal screening of phenylketonuria, galactosemia, maple syrup urine disease, homocystinuria, histidinemia & congenital hypothyroidism for newborns delivered from low class pregnant women registered in health centers. Government decreased the test items from six to two, PKU & congenital hypothyroidism to increase test numbers with same budget from 1995. Government decided to test PKU & hypothyroidism for all newborns from 1997. 78 laboratories wanted to participate for neonatal screening test in 1999. Government didn't decide laboratory center for a certain district and placed responsibility on free competition. Government are planning to test 573,000 newborns from 1998, Government decided to screen 6 items PKU, congenital hypothyroidism, maple syrup urine disese, homocystinuria, galactosemia and congenital adrenal hyperplasia from 2006. 17 laboratores are participating now. The cost of screening test is supported by both the federal government and local government on a 40-60 basis. In case a patient with an inherited metabolic disease is diagnosed by screening of government program, special milk is provided at government's expense. Interlaboratory quality control was started 6 times a year from 1994. According to the government project, 3,707,773 newborns were screened. 86 PKU, 718 congenital hypothyroidism were detected. So incidence of PKU is 1/43,114 and congenital hypothyroidism is 1/4,612. Maeil dairy company produced new special formula for PKU, MMA and PA, MSUD, urea cycle disorder, homocystinuria, isovaleric acidemia from Oct. 1999. The cost benefit of performing screening procedures coupled with treatment has been estimated to be as high as 1.77 times in PKU, 11.11 times in congenital hypothyroidism than cost without screening. We are trying to increase the budget to test all newborns for Tandem mass sereening & Wilson disease from 2008. Now it is a very important problem to decrease laboratory numbers of neonatal screening in Korea. So we are considering 4-5 central laboratories which cover all newborns and are equipped with tandem mass spectrometer & enzyme immunoassay for TSH, 17OHP & enzyme colorimetric assay for galactose.

• Clinical and Experimental Reproductive Medicine
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• 제21권3호
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• pp.227-232
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• 1994

In order to define the clinical characteristics of Korean women with polycystic ovary syndrome, clinical symptoms, biochemical features and ultrasonographic findings were determined in ninety PCO patients. Frequency of clinical manifestations were as follows:acne 42%, overweight 31 %, oily skin 14 %, hirsutism 10%. Relatively low frequencies of hirsutism is recognized in patients with Korean PCO syndrome. Mean(${\pm}$S.E.) of each hormone was:testosterone 1.18${\pm}$1.07ng/ml, LH 21.47${\pm}$${\pm}$2.6mIU/ml, FSH 7.26${\pm}$2.67mIU/ml, LH/FSH ratio 2.94${\pm}$1.29, prolactin 25.48${\pm}$46.33ng/ml, DHEA-S 333.78${\pm}$309.60ng/dl, 17-0HP 1.72${\pm}$1.74ng/ml. Mean 17-OHP after ACTH stimulation test was 5.07${\pm}$12.01ng/ml. Ultrasonographically measured mean ovarian volume were $11.02{\pm}5.92cm^3$ in right and $9.23{\pm}5.64cm^3$ in left and small sized multiple subcapsular follicular cysts were noted in 43 patients (47.9%) with PCO syndrome.

• 한국수산과학회지
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• 제31권4호
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• pp.599-607
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• 1998

동사리, Odontobutis platycephala 암컷의 생식주기를 밝히고자 1995년 12월부터 1991년 11월까지 생식소 중량지수(gonadosomatic iadex GSI), 난소의 난소여포 발달 및 혈중 성스테로이드 호르몬 변화 양상을 조사하였다. GSI는 7월과 8힐에 0.35$\~$0.72였으며, 난소내 대부분의 난모세포들은 염색인기와 주변인기 단계였다(출현 빈도: $87\%\~96\%$). 9월의 GSI는 1.20$\pm$0.12였으며, 일부의 난황포기의 난모세포들과 난황형성기의 난모세포도 출현하였으나 극히 일부에 지나지 않았다(각각의 출현 빈도:$22.8\%,\;2.2\%$). 10월부터 GSI는 완만하게 증가하여 12월 4.59$\pm$0.61에 달하였으며 난황형성기 난모세포들이 약간 증가하였다(12월의 출현 빈도: $22.1\%$). 1월에는 GSI의 큰 변화는 없었으나 난황형성기 난모세포들은 12월보다 더 증가하였다(출현 빈도: $51.2\%$). 2월부터 GSI는 급격히 증가하여 3월에 10.51$\pm$1.04로 연중 가장 높았으며, 난소의 난황형성기 난모세포들도 연중 최고 수준에 이르렀다(3월의 출현 빈도. $60\%$). 4월부터 GSI는 감소하여 6월에는 1.11$\pm$0.35였으며 성숙기의 난모세포를 가진 난소여포들과 배란 후 퇴화되는 난소여포들이 나타났고, 이들의 출현 빈도는 4월에 가장 높았다(성숙기 난모세포출현 빈도: 4월 $40\%$, 5월 $12\%$, 6월 $5\%$). Estradiol-17$\beta$($E_2$)의 혈중 농도는 8월에 0.84$\pm$0.20$ng/m{\ell}$로 12월까지는 비슷한 수준에 머물렀으나 1월부터는 증가하여 3월에 2.85$\pm$0.35$ng/m{\ell}$로 연중 가장 높았으며, 4월부터는 감소하여 7월에 0.14$\pm$0.02$ng/m{\ell}$로 가장 낮았다(P<0.05). 17$\alpha$-hydroxprogesterone(17$\alpha$-OHP)의 혈중 농도는 3월에 13.37$\pm$0.52$ng/m{\ell}$로 높았을 뿐 다른 시기동안은 $3ng/m{\ell}$이하로 유의한 변화를 보이지는 않았다(P<0.05). 17$\alpha$, 20$\beta$-dihydroxy-4-pregnen-3-one(17$\alpha$, 20$\beta$-P)의 혈중 농도는 4월에 0.74$\pm$0.09$ng/m{\ell}$로 최고 수준이었고 5월까지는 0.54$\pm$0.07$ng/m{\ell}$로 높은 농도를 유지했지만 그외의 시기에는 0.26ng/ml 이하로 연중 유의한 변화는 없었다(P<0.05). 이러한 결과를 종합하면, 동사리의 완숙 및 산란기는 4월$\~$6월로서 주산란기는 4월과 5월 사이이며, 7월$\~$8월은 휴지기, 9월$\~$12월은 성장기, 1월$\~$3월은 성숙기로 구분되는 생식주기를 보였으며, 이러한 변화는 성스테로이드 호르몬의 변화양상과 밀접한 관련이 있는 것으로 생각된다.

• 대한유전성대사질환학회지
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• 제14권1호
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• pp.1-9
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• 2014

Many inborn errors of metabolism can be completely cured with early detection and early treatment. This is why neonatal screening on inborn errors of metabolism is implemented worldwide. In 1991, the Ministry of Health & Social affairs adopted a nationwide service program for neonatal screening of phenylketonuria, galactosemia, maple syrup urine disease, homocystinuria, histidinemia and congenital hypothyroidism for newborns delivered from low class pregnant women registered in health centers. Government decreased the test items from six to two, PKU and congenital hypothyroidism to increase test numbers with same budget from 1995. 78 laboratories wanted to participate for neonatal screening test in 1999. Government decided to screen six items of PKU, congenital hypothyroidism, maple syrup urine disease, homocystinuria, galactosemia and congenital adrenal hyperplasia from 2006. In 2014, thirteen laboratories are participating. Inter laboratory quality control was started 6 times a year from 1994. In case a patient with an inherited metabolic disease is diagnosed by screening of government program, special milk is provided at government's expense. According to the government project, from 1997 to 2013, 7,080,569 newborns were screened. 144 PKU, 2.451 congenital hypothyroidism were detected. So incidence of PKU is 1/49,170 and congenital hypothyroidism is 1/2,888. The cost benefit of performing screening procedures coupled with treatment has been estimated to be as high as 1.77 times in PKU, 11.11 times in congenital hypothyroidism than cost without screening. By January 2007, many European countries had expanded of their newborn screening programs by inclusion of Tandem mass spectrometry. We are trying to increase the budget to test all newborns for Tandem mass spectrometry from 2016. We are considering four to five central laboratories which cover all newborns and are equipped with tandem mass spectrometer & enzyme immunoassay for TSH, 17OHP & enzyme colorimetric assay for galactose. And I hope to expand test including Wilson disease screening test and lysosomal storage diseases.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7105-7112
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• 2014

In this study, we investigated oxidative stress and tumor marker levels of polycyclic aromatic hydrocarbons (PAHs) in 136 coke oven workers and in 60 control subjects, and evaluated the correlation between oxidative stress and tumor marker levels. Questionnaires on basic demographic information were also administered. Significant differences in employment time and percentages of alcohol drinkers were observed between the control and exposed groups. PAH exposure was assessed using urinary 1-hydroxy-pyrene (1-OHP) levels and was found to be significantly higher in workers than in the controls. Significant differences (P<0.001) of MDA, GST, LDH, NSE, Cyfra21-1, and of SCC and TNF-a (P<0.0001 and P<0.05, P<0.001, respectively) levels were observed among controls and coke-oven workers, except for bottom coke oven workers. Associations between age and risk of increased TNF-a, smoking and increased GST activities, and drinking with increased MDA concentrations, were marginal (P=0.055, P=0.048, P=0.057, respectively). The association between smoking with MDA (P=0.004), NSE (P=0.005), SCC (P=0.004) andTNF-a (P<0.001), and drinking with TNF-a levels was significant (P=0.012). In addition, a significant positive correlation between oxidative stress and tumor markers was found in the present study. These results suggest that a synergistic increase of oxidative stress and tumor markers induced by PAHs may play a role in toxic responses for PAHs in coke oven workers.

• 핵의학기술
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• 제13권3호
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• pp.159-164
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• 2009

목적 : 본원에서 시행하고 있는 혈액 검사 경고치 자동통보 시스템의 목적은 환자의 혈액 검사 결과에 따라 신속한 치료를 받아야 할 상황이 발생되면 구축된 자동통보 시스템을 통해 즉시 환자 주치의에게 결과를 알려주고, 이에 따른 적절한 처치가 시행되어 환자에게 발생될 수 있는 치명적 영향을 줄이기 위함이다. 최근 핵의학과 혈액 검사실에서도 TSH, FT4, $17{\alpha}$-OHPGR 3종목에 대해 혈액 검사 경고치 자동통보 시스템을 실시함에 따라 운영현황을 소개 하고자 한다. 실험재료 및 방법 : 대상은 2009년 2월 19일부터 5월 11일까지 신생아과로부터 의뢰된 TSH, FT4, $17{\alpha}$-OH-PGR 처방환자 중 다음 기준에 해당하는 환자들로 하였다. N2340 Thyroid-Stimulating Hormone: >$10{\mu}IU/mL$(참고치: 0.4~5.0) N2360 Free-Thyroxine: <$0.8{\mu}g/dL$(참고치: 0.8~1.9), N2444 $17{\alpha}$-OH-Progesterone: >$30{\mu}g/dL$(참고치: 남성(0.6~3.42), 여성 난포기(0.19~1.8)) 방법은 OCS Program에 대상 검사 종목, 진료과, 경고치보고 기준 값을 입력해 놓은 후 확정된 결과가 입력된 조건에 해당하면 처방의 및 주치의의 PDA로 SMS를 전달하게 된다. 결과 : 경고치 자동통보 시스템으로 보고된 결과는 2009년 2월 19일부터 3월 31일까지 총15건이었고 2009년 4월 1일부터 5월 11일까지 총 18건으로 3건의 증가가 있었다.

• 한국간호교육학회지
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• 제3권2호
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• pp.255-275
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• 1997

This study was conducted to find out the effect of sexual education for knowledge and attitude toward sex are elementary school students. Therefore, the present study aims to understand the changes of the knowledge are the attitude toward sex before and after the sex education, in elementary school students and then finally to provide some basic data to suggest directions for sex education. The subjects were 500 male and female elementary students from the fifth and sixth grades of 4 public elementary schools in Pusan. The subjects were assigned to a experimental (250) and a control (250) group. The research tools were sex education program and consists of physiology(menache, ejaculation), psycological(pleasure of growth), sociological(hetero sexual relationship) are about set The sex education program was given to experimental group for 6 hours by the trained school nurse's lecture using slide film and OHP film. The data were obtained before and after the sex education. Data collection was done during the period betwen Feb. 3. 1997. to Feb. 20. 1997. The collected data were analyed using frequency, percentages, $X^2$-test, means, standard deviation, t-test, ANOVA, MANCOVA with SPSS program. The results were obtained as follows : 1. The first hypotheses attained that the experimental group(19.72) is higher than the control group(12.12) on the knowledge toward sex(F=467.30, P=.000). At the physical area, the experimental group(8.84) is higher than the control group(3.96) (F=687.39, P=.000). At the psychological area the experimental group (5.18) is higher than the control group(2.63) (F=411.66, P=.000). At the social area, the experimental group (5.70) is higher than the control group (5.54), this is not significant difference (F=.67, P=.413). 2. The second hypotheses attained that the experimental group(60.42) is higher than the control group(52.48) on the attitude toward sex(F=215.70, P=.000). At the physical area, the experimental group(23.10) is higher than the control group(20.68) (F=128.68, P=.000), At the psychological area, the experimental group(20.11) is higher than the control group(16.40) (F=108.64, P=.000). At the social area, the experimental group(17.22) is higher than the control group(16.00), this is area are significant differences (F=70.37, P=.000). 3. Knowledge toward sex according to sex, religon, atmosphere of family, mother age and educational background are significant differance (P<0.05). Attitude toward sex according to sex is significant differance (P<0.05).

• 한국수산과학회지
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• 제30권2호
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• pp.282-290
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• 1997

Vitellogenin 합성 메카니즘을 밝히기 위한 연구의 일환으로써, 뱀장어의 간세포배양계를 이용하여 Vg합성에 대한 $E_2$ 및 뇌하수체호르몬들의 영향에 대해 조사하였다. 일반적으로 어류를 비롯한 난생 척추동물들의 Vg은 $E_2$의 자극에 의해 간장에서 합성분비되어 지나 뱀장어 Vg은 $E_2$으로는 충분한 합성은 이루어지지 않았다. 그러나 $E_2$와 GH 및 PRL을 함께 첨가하면 Vg합성이 활발하게 유도되어 지는 것이 관찰되었다. $E_2$ 만으로 Vg이 합성되어지지 않는다면 세포내에 Vg합성 및 $E_2$ 의 활성을 억제하는 내인성 요소들이 있을 것으로 생각되어 세포를 8일간 호르몬 첨가없이 preculture하여 시행하였으나, $E_2$만으로 Vg합성은 유도되어 지지 않고 GH 및 PRL의 도움이 필요했다. 또한 $E_2$ 만으로 10일간 배양하여 장기간 $E_2$ 로 노출시켜 Vg합성에 대한 영향을 조사한 바, 약 10일간의 $E_2$ 노출에 의해 Vg합성은 전기영동상의 확인에 의해 양적으로 다소 증가하는 것이 관찰되었으나 $E_{2}+GH$ 및 $E_{2}+PRL$의 첨가에 비해 현저한 차이를 나타내어 뱀장어의 Vg합성 유도에는 뇌하수체 호르몬들이 직접 관련되는 것으로 나타났다. 또한 $E_{2}+GH+PRL$을 함께 첨가하여 6일간 배양하여 Vg이 왕성하게 합성되어 질때에도 계속해서 GH 및 PRL들이 필요한가를 조사하였는데, 3일간 관찰한 결과 Vg의 합성 정도는 GH 및 PRL등의 첨가유무에 커다란 차이를 나타내지 않았다.는 초기균수 $3.4{\times}10^3\;CFU/g$ 및 $3.4{\times}10^4\;CFU/g$에서 각각 $2.8{\times}10^4\;CFU/g$와$9.8{\times}10^5\;CFU/g$로 $1\~1.5-log-unit$가 증가하였다.평가는 0.005였다.5IU/ml의 배지에서는 대조구의 배란율과 차이를 보이지 않았다. 이러한 결과로 $E_2$를 제외한 스테로이드와 HCG는 동자개의 난모세포 성숙과 배란을 in vitro에서 유도할 수 있으며, $17\alpha\;20\alpha\;OHP$와 HCG는 다른 스테로이드에 비해 높은 율의 난모세포 성숙과 배란을 유도하였다.체로 잘 일치하였다. 또한 진해만의 저서동물 군집의 전반적인 계절별 공간분포는 다양도 분석 결과에서도 분명하게 나타나고 있으며, 주로 퇴적물 내의 유기물 함량에 의해 영향을 받고 있음을 알 수 있었다., Chlorella sp., yeast를 rotifer에 먹이로 투여했을 때 $S\;\pi-I$에서 lysine이 가장 높았고, PSB, Chlorellaal sp. 순으로 분석되었다. yeast에서는 검출되지 않았다./TEX>, betaine류$(0.1\~0.9\%)$의 순이었다. 연어 엑스분중의 질소분포 특징은 모든 시료에서 공통적으로 anserine과 creatine이 중요한 함질소 성분으로서 엑스분 질소에 대한 양 성분질소의 비율은 anserine이 평균 $38\%$, creatine이 평균 $31\%$로서 이 두 성분만으로 엑스분질소의 $60.9\~76.5\%$에 달하였다. 분석된 성분에 의한

• Clinical and Experimental Reproductive Medicine
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• 제40권3호
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• pp.135-140
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• 2013

Objective: To evaluate correlations between serum anti-M${\ddot{u}}$llerian hormone (AMH) levels, phenotypes of polycystic ovary syndrome (PCOS), obesity, and metabolic parameters in patients with PCOS. Methods: A total of 175 patients with PCOS were diagnosed according to the Rotterdam Consensus were included. Exclusion criteria were age over 40, FSH>25 mIU/mL, and 17a-OHP>1.5 ng/mL. The Phenotypes of PCOS were divided into a severe form (oligo-anovulation, ANOV/hyperandrogenism/polycystic ovary morphology [PCOM]; n=59) and a mild form without HA (ANOV/PCOM, n=105). The serum AMH levels were classified into 3 groups (<5 vs. 5-10 vs. >10 ng/mL). Obesity was defined as body mass index (BMI) ${\geq}25kg/m^2$ (n=34). Results: The mean age was $25.9{\pm}5.7$ year and mean AMH level was $10.1{\pm}5.4$ ng/mL. The BMI ($kg/m^2$) was higher in group 1 ($24.2{\pm}6.3$) than in group 2 ($21.9{\pm}4.3$, p=0.046) or group 3 ($21.6{\pm}3.3$, p=0.019). There was no difference among the three groups in age, menstrual interval, antral follicle counts, androgens, or other metabolic parameters. The obesity group showed significantly lower AMH ($7.7{\pm}3.9$ ng/mL vs. $10.7{\pm}5.6$ ng/mL), p=0.004) and low-density lipoprotein levels ($93.1{\pm}21.2$ mg/dL vs. $107.5{\pm}39.3$ mg/dL, p=0.031), and showed higher total T ($0.74{\pm}0.59$ L vs. $0.47{\pm}0.36$ ng/mL, p=0.001), free T ($2.01{\pm}1.9$ vs. $1.04{\pm}0.8$ pg/mL, p=0.0001), and free androgen index ($6.2{\pm}7.9$ vs. $3.5{\pm}3.0$, p=0.003). After controlling for age factors and BMI, the serum AMH levles did not show any significant correlations with other hormonal or metabolic parmeters. Conclusion: For PCOS patients under the age 40, serum AMH is not negatively correlated with age. High serum AMH levels can not predict the phenotype of PCOS and metabolic disturbances in PCOS patients in the non-obese group. Further study might be needed to define the relation more clearly.

• Child Health Nursing Research
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• 제7권1호
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• pp.118-140
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• 2001

The purpose of this study was to measure the effect of safety education program in preschool children for accident prevention and improve their health through more systematic method. Data were collected from 584 preschoolers(247 preschooler are assigned to experimental group and 337 preschoolers are assinged to control group) from 4 to 6 years old using APP paper test which consists of questions and drawings. To experimental group, safety education were done 4 times within the time of 30 minutes per 1 time using education books, drawings, OHP. The findings of this study are as follows: 1. There were significant difference in movement(χ²=18.732, p=.0000), behavioral character(χ²=27.785, p=.000), synthetic judgement(χ²=12.02, p=0.002). So, safety education program have effect on preschooler. 2. In the accident proneness on preschooler between experimental group and control group according to general characteristics, it proved significant difference in the case of accident prevention education were done, reasoning power(χ²=10.48, p=.005), movement speed(χ²=7.341, p=.025) and behavioral character(χ²=18.86, p=.000), in the case of housing pattern is private house(individual house, yard?), reasoning power(χ²=6.683, p=.035), movement speed(χ²=12.76, p= .002) and behavioral character(χ²=12.24, p=.002), in the case of housing pattern is mixed-type, movement speed(χ²=6.935, p= .031) and behavioral character(χ²=10.816, p=.004), in the case of housing pattern is over six stories, movement speed(χ²=7.543, p=.023), in the case of subjects' age is 4 years old, movement speed(χ²=16.5, p= .000) and behavioral character(χ²=12.18, p=.002), in the case of subjects' age is 5 years old, movement speed(χ²=7.519, p= .023), watchfulness(χ²=6.372, p=.041), behavioral character(χ²=14.74, p=0.001) and synthetic judgement(χ²=14.5, p=.001), in the case of subjects' sex is male, life safety(χ²=6.406, p=.041), movement speed(χ²=22.86, p= .000), behavioral character(χ²=13.72, p= .001) and synthetic judgement(χ²=13.82, p=.001), in the case of subjects' sex is female, reasoning power(χ²=12.57, p=.002) and behavioral character(χ²=13.16, p= .001), in the case of childrens have past accidental experience, traffic safety(χ²= 6.683, p=.035), in the case of childrens have no past accidental experience, reasoning power(χ²=8.384, p=.015), movement speed(χ²=20.6, p=.000), behavioral character(χ²=25.1, p=.000) and synthetic judgement(χ² =10.79, p=.005), in the case of children's order is first, reasoning power(χ²=11.15, p=.004), movement speed(χ²=11.92, p= .003) and behavioral character(χ²=7.003, p=.030), in the case of children's order is second, movement speed(χ²=6.694, p= .035), behavioral character(χ²=26.9, p= .000) and synthetic judgement(χ²=14.3, p= .001), in the case of nuclear family, reasoning power(χ²=8.777, p=.012), movement speed(χ²=19.0, p=.000), behavioral character (χ²=26.4, p=0.000) and synthetic judgement (χ²=9.999, p=.007), in the case of mothers' school career is under high school graduate, life safety(χ²=8.023, p=.018), movement speed(χ²=10.99, p=.004) and behavioral character(χ²=6.777, p=.034), in the case of mothers' school career is beyond college graduate, reasoning power(χ²=6.717, p= .035), movement speed(χ²=8.963, p=.011), behavioral character(χ²=25.03, p=.000) and synthetic judgement(χ²=15.19, p=.001), in the case of mothers' age ranged 31-34, movement speed(χ²=12.29, p=.002) and behavioral character(χ²=14.17, p=.001), in the case of mothers' age ranged 35-39, movement speed(χ²=9.859, p=.007), behavioral character(χ²=9.095, p=.011) and synthetic judgement(χ²=7.810, p=.020), in the case of mothers' age is over 40, life safety(χ² =5.593, p=.025), in the case of mothers' job is full-time, traffic safety(χ²=6.032, p=.049) and reasoning power(χ²=8.502, p= .014), in the case of mothers' job is part- time., movement speed(χ²=10.99, p=.004) and behavioral character(χ²=7.895, p= .019), in the case of mothers have no job, movement speed(χ²=6.410, p=.041), movement stability(χ²=6.879, p=.032), behavioral character(χ²=27.72, p=.000) and synthetic judgement(χ²=18.11, p=.000). The difference of accident proneness between experimental group and control group according to general characterists, it also showed that there were significant difference in behavioral character compared to other area.. From this findings, we can guess that safety education program change and guide preschoolers' behavioral character to desirable direction.