• Title/Summary/Keyword: 17-KS

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Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs

  • Han, Yan-Guo;Liu, Gui-Qiong;Jiang, Xun-Ping;Xiang, Xing-Long;Huang, Yong-Fu;Nie, Bin;Zhao, Jia-Yu;Nabeel, Ijaz;Tesema, Birhanu
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.6
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    • pp.835-841
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    • 2018
  • Objective: The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor (KISS1) gene vaccine in immunocastration. Methods: Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0, 3, and 6 of the study. Blood samples were collected from the jugular vein before primary immunization and at weeks 2, 4, 6, 10, 14, 22, and 30 after primary immunization. All ram lambs were slaughtered at 38 weeks of age, and samples were collected. Results: The specific anti-KISS1 antibody titers in vaccinated animals were significantly higher and the serum testosterone level was significantly lower than those in the control groups from week 4 to 14 after primary immunization (p<0.05). No significant difference was observed at weeks 22 and 30 after the primary immunization. Similar results were also found for scrotal circumference, testicular weight, length, breadth, and spermatogenesis in seminiferous tubules in week 30 after primary immunization. KS (KISS1-hepatitis B surface antigen S) fusion fragment of KISS1 gene vaccine was not detected in host cell genomic DNA of 9 tissues of the vaccinated ram lambs by polymerase chain reaction. Conclusion: The effects of KISS1 gene vaccine in immunocastration were reversible and no integration events were recorded.

A Study on the Behavior of the Free Space Scatter dose in X-ray Diagnostic Room (X선촬영실 내에서의 공간산란선량 변동에 관한 연구)

  • Oh, Hyun-Joo;Kim, Sung-Soo;Kim, Young-Il;Lim, Han-Young;Kim, Heung-Tae;Lee, Who-Min;Kim, Hak-Sung;Lee, Sang-Suk
    • Journal of radiological science and technology
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    • v.17 no.2
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    • pp.21-27
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    • 1994
  • In this pauper, when the X-ray exposure condition is 70, 90, 110 kV, 10 mAs, FFD 180 cm, FSO $10{\times}10$, $35{\times}35\;cm$, toward the $36{\times}36{\times}15\;cm$ acryl phantom, the free space scatter dose rate at the 15th points in X-ray diagnostic room was measured by electrometer and 1800 co ionization chamber. Therefore, the free space scatter dose distribution profile was drown, and then, the free space scatter dose contribution percentage was Investigated. The obtained results are summarized as following. 1. The X-ray tube leakage dose rate of the experiment generator at the 1 m from focus was measured maximum 85 mR/hr, minimum 20 mR/hr, therefore, this values was appeared below the KS rules, 2. The free space scatter dose become to larger at the primary X-ray beam around area, and lower at the back ward X-ray tube. The maximum values were 3,812 mR/hr at the front Lt 1 m $45^{\circ}$ point, minimum 117 mR/hr at the back ward 1 m $180^{\circ}C$ point. 3. As the more tube voltage and field size increase, the more free space scatter dose contribution percentage become to increase, as to 90 kV from 70 kV, increase to 12 %, to 110 kV from 90 kV, increase to 18 %, and then, become to 11 % at the $10{\times}10\;cm$ and 87 % at the $35{\times}35\;cm$. 4. The 89 % of the total producted scatter ray occured from acryl phantom, at the X-ray tube housing 6 %, at the front side back wall 5 %. 5. The free space scatter dose contribution percentage at the one point build up 80 % from the phanton direction, 14 % from the X-ray tube and collimator direction, 2.2 % from the front wall, 1.8 % from the side wall, 1.7 % the back wall.

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Prevalence of Antibodies to Human Herpesvirus 8 in Children (소아의 항 Human Herpesvirus 8 항체 양성률)

  • Han, Tae Hee;Chung, Ju Young;Kim, Sang Woo
    • Pediatric Infection and Vaccine
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    • v.12 no.2
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    • pp.108-113
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    • 2005
  • Purpose : Human herpsevirus 8(HHV-8), a gamma herpsevirus, was initially identified from Kaposi sarcoma(KS) lesions and has been known to be associated with several malignancies including Kaposi sarcoma. HHV-8 seroprevalence is variable by different geographic areas and populations. The prevalence of HHV 8 infection in Korean children is unclear. So, we investigated the prevalence of HHV-8 specific antibodies in healthy children in Seoul, Korea. Methods : Sera were obtained from 112 children(age 1~15 years, 64 males and 48 females) who visited our hospital for routine health checkup and used for investigating sero-prevalence of anti-HHV-8 antibodies. An indirect immunofluorescent assay was used to detect the IgG antibodies to the lytic viral antigen(Biotrin, Dublin, Ireland). A peptide mix ELISA kit was used to detect the IgG antibodies to peptides specific for HHV-8 open reading frame (ORF)(Biotrin, Dublin, Ireland). Results : Of 112 children, 4 children younger than 6 years of age were seropositive to HHV-8[all 4(3.5%) were positive by IFA and 2(1.8%) were positive by ELISA]. Conclusion : These results suggest that the prevalence of antibody to HHV 8 in children in Korea is very low.

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Dielectric properties of $0.6Pb(Sc_{1/2}Ta_{1/2})O_3-0.4PbTiO_3$ ceramics prepared by the molten salt synthesis method (용융염 합성법에 의해 제조된 $0.6Pb(Sc_{1/2}Ta_{1/2})O_3-0.4PbTiO_3$ 세라믹스의 유전성)

  • Park, Kyung-Bong;Kim, Tae-Huei;Kwon, Seung-Hyup;Lim, Dong-Ju
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.17 no.2
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    • pp.69-74
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    • 2007
  • [ $0.6Pb(Sc_{1/2}Ta_{1/2})O_3-0.4PbTiO_3$ ] (hereafter PSTT) ceramics were prepared by the molten salt synthesis (MSS) method using KCI as a flux. Formation of perovskite phase was investigated by a differential thermal analysis (DTA) and X-ray diffraction (XRD) analysis in the temperature range from $600^{\circ}C$ to $850^{\circ}C$. A 92% perovskite phase was synthesized at $750^{\circ}C$ for 2 hrs using the MSS method, while 82% perovskite phase was synthesized at $850^{\circ}C$ for 4ks using the calcining of mixed oxide (CMO) method. This result could be due to the improvement in reactivity of $Sc_2O_3$ by melting of KCI. The MSS specimen sintered at $1,100^{\circ}C$ for 4hrs showed a dielectric constant of 11,200, a remnant polarization of $13.5{\mu}C/cm^2$ and a coercive field of 10.198 kV/cm, which was discussed in view of the microstructure.

The Effect of Environmental Factors on the Advent of Chattonella (Raphidophyceae) in Yeosu Coastal Waters, Korea, and the Effect of Nutrients on the Growth of Chattonella (여수 연안해역에서 침편모조류 Chattonella속 출현환경 및 영양염에 대한 성장특성)

  • Noh, Il-Hyeon;Oh, Seok-Jin;Shin, Hyeon-Ho;Kang, In-Seok;Yoon, Yang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.4
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    • pp.362-372
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    • 2010
  • In order to understand what leads to the appearance of harmful Chattonella algae in the Yeosu coastal waters of Korea, we measured environmental parameters every week at one station from May to November, 2006, and April to October, 2007. Four species of Chattonella appeared during the monitoring period: C. antiqua, C. globosa, C. marina and C. ovata. The range of water temperature and salinity were $15.0-27.9^{\circ}C$ and 17.6~33.0 psu, respectively, when Chattonella appeared, and their maximum cell density (4,840 cells/L) was at $27.1^{\circ}C$ and 33.0 psu. During the monitoring periods, the range of dissolved inorganic nitrogen (DIN), phosphate (DIP) and chlorophyll $\alpha$ (Chl-$\alpha$) concentrations in surface waters were $1.20-52.23\;{\mu}M$ ($8.59{\pm}8.97\;{\mu}M$), $0.03-1.56\;{\mu}M$ ($0.47{\pm}0.31\;{\mu}M$) and $0.45-31.12\;{\mu}g/L$ ($3.58{\pm}4.77\;{\mu}g/L$), respectively. Chattonella occurred at low cell density when the Chl-$\alpha$ concentration increased because of supplied nutrients, whereas their cell density increased during the periods of rapid decrease in Chl-$\alpha$. The results of growth experiments based on batch culture showed that the half saturation constant ($K_s$) of C. antiqua on ammonium (${NH_4}^-$), nitrate (${NO_3}^-$) and phosphate (${PO_4}^{2-}$) were $3.89{\mu}M$, $5.01\;{\mu}M$ and $0.63\;{\mu}M$, respectively. These Ks values are higher than those reported for diatoms and other flagellates at the DIP concentration (average $0.47{\mu}M$) of Yeosu coastal waters. Although the maximum specific growth rate (${\mu}_{max}$) of C. antiqua was lower than diatoms, it was higher than those of other flagellates. Therefore, our results indicate that the DIP level in the study area was too low to support Chattonella blooms, although Chattonella species have physiological characteristics that enable them to grow more rapidly than other flagellates when nutrient levels are higher than their $K_s$.

Rapid Determination of Ginsenosides Rb1, Rf, and Rg1 in Korean Ginseng Using HPLC (HPLC를 이용한 고려인삼 중 진세노사이드 Rb1, Rf 및 Rg1의 신속분석 방법 개발)

  • Hong, Hee-Do;Choi, Sang-Yoon;Kim, Young-Chan;Lee, Young-Chul;Cho, Chang-Won
    • Journal of Ginseng Research
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    • v.33 no.1
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    • pp.8-12
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    • 2009
  • A simple gradient HPLC method for rapid determination of major ginsenosides ($Rg_1$ and $Rb_1$) and unique ginsenoside (Rf) of Korean ginseng (Panax ginseng C.A. Meyer) was developed. Within 50min, three ginsenosides have been separated and identified on $\mu$-Bondapak $C_{18}$ column ($3.9{\times}300\;mm$, $10{\mu}m$) with gradient elution using water and acetonitrile as a mobile phase. The method was validated in terms of linearity, accuracy, and precision. The correlation coefficients ($r^2$) for calibration curves of ginsenosides were over 0.9997. The developed HPLC method was successfully applied to the analysis of ginseng samples and the recoveries of ginsenosides were in the range of $101.1{\sim}115%$ with RSD<3.2%. The developed method could be used for rapid evaluation of the ginsenosides $Rg_1$, $Rb_1$, and Rf.

A Comparative Study on Sleep State, Sleep Time, and Quality of Life of Vietnam Migrant Women by Sasang Constitution (베트남 이주여성의 사상체질별 수면상태, 수면시간 및 삶의 질 비교연구)

  • Jang, Eunsu;Kim, Yunyoung;Baek, Younghwa;Moon, Sujeong;Lee, Siwoo;Kim, Sang-Hyuk
    • Journal of Sasang Constitutional Medicine
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    • v.32 no.1
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    • pp.39-49
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    • 2020
  • The purpose of this study was to find out how sleep characteristics by Sasang constitution (SC) appear in Vietnamese immigrant women in Korea, and how sleep aberration by SC affected the quality of life. The number of 217 Vietnamese immigrant women joined this study. We surveyed SC with Korea Sasang Constitutional Diagnostic Questionnaire (KS-15) and sleep characteristics with Pittsburgh Sleep Quality Index(PSQI) questionnaire. We evaluated quality of life(QoL) with Euro Quality of Life-5Dimension(EQ 5D) questionnaire. A Chi-square test and a one-way ANOVA test were conducted according to SC, and T test also used to compare PSQI groups. Significant p was .05. The number(rate) of Taeeumin(TE), Soeumin(SE), and Soyangin(SY) were 40(18.4%), 55(25.3%), and 122(56.2%) respectively. The distribution of obstructive factors of sleep were significantly different according to SC(Too Cold in Sleeping, x =13.378, p=.037, Too Hot in Sleeping x2=12.834, p=.046). The distribution of daytime drowsiness(x2=15.379, p=.018) and falling concentration power (x2=17.533, p=.008) was significantly different according to SC. The sleep status of the subjects showed a significant negative correlation with the EQ 5D Visual Analogue Scale(VAS) (r =-.399, p<.001), and the EQ 5D Index (r=-.211, p=.002). In the EQ VAS, TE showed a difference between the good and bad sleeping groups (t=3.955, p<.001). SE showed a difference between the two groups in the EQ 5D Index (t=2.332, p=.037). SY did not show a difference between the two groups. This study reveals that sleeping is closely related with QoL and distribution of obstructive factors of sleep may be different according to SC. Further study to confirm the constitutional difference in race at Vietnam is needed.

A Study on the Preventive Measures against Fall Injuries in Manufacturing Industry Focusing on the Portable Ladders (이동식 사다리를 중심으로 한 제조업에서의 추락재해 예방대책 연구)

  • Kim, Hyung-Seok;Lee, Seok-Won;Jung, Won-Jae;Ryu, Bo-Hyuk
    • Journal of the Korean Society of Safety
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    • v.24 no.6
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    • pp.136-143
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    • 2009
  • The injuries as a result of fall accidents is the one of the most common kind of injuries in Korea, especially fall from height is the top most common kind for fatal injuries. Futhermore, the number of fall injuries in manufacturing industry has been steadily increasing from 2000 to 2007. In 2007 the number of fall fatal injuries increased 10.6 % in comparison with the number in 2006. These numbers are 6~17 times higher than those for foreign countries such as the U.K and the USA. So, it is needed to make a study to reduce these numbers for manufacturing industry. To get the goals, we did research as followings. First, this study analyzed statistics by industry, by fall height, by common agent and so on to gain an insight into real aspect of fall injuries. It showed that ladder is the commonly cited agent for manufacturing industry, and 3 risky activities ; the ladder work, the load/unload work, and the maintenance work. Secondly, this study made a survey of manufacturing workers for their understandings of the most 3 risky activities. It showed that 'tendency to work easily' and 'difficulty of applying measures due to inherent working conditions' are main reasons for workers not to implement the preventive measures against the fall accidents. Thirdly, this study tested the slip characteristics of ladder base and lungs. The tests were done based on ANSI standard and KS. In addition to the standard condition, this study performed slip tests in wet and sandy conditions and compared the results with the those of standard condition. It showed that friction coefficient is standard condition > wet condition > sandy condition, and the friction coefficient of ladder for sandy condition is measured to be reduced by 63.9% compared with that for standard condition. Finally, this study developed safety work models for using of portable ladders in consideration of the safety standards of foreign countries such as the U.K, the USA, and the Australia.

Introduction and development process of SPS (단체표준(SPS) 소개와 개발 프로세스)

  • Tae Hyeong Kim;Se Hyun Park;Dae Sung Jung
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.6-6
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    • 2023
  • 국토교통부는 R&D 사업인 '분산공유형 건설연구인프라구축사업(2004년~2009년, 6종)'과 '국토교통기술실험인프라 2단계 구축사업(2013년~2019년, 6종)'을 통해 첨단 연구시설·장비를 전국에 분산 구축하여 실험시설이 부족한 중소기업·대학 등이 공동활용할 수 있도록 총 12종의 건설분야 대형실험시설을 구축하였다. 국토교통 R&D 성과(기술·제품)의 품질 및 신뢰성 확보를 통해 개발기술의 상용화 촉진을 위해서는 이러한 국토교통 대형실험시설·장비를 활용한 표준화된 성능평가 시험기반(표준실험절차, 단체표준 등)을 구축하여 연구인프라의 공동활용을 촉진하고 기술개발 성공률 제고와 함께 건설분야 시험인증 산업을 육성할 필요가 있다. 국토교통 대형실험시설을 활용하여 건설분야 기술·제품에 대한 성능평가 기반의 실험절차를 표준화함으로써 성능평가 결과의 신뢰성 확보 및 기술시험서비스 역량 제고를 위한 '건설분야 기술·제품의 검증을 위한 성능기반 표준실험절차 개발(연구기간: '17.06~'22.06, 연구비: 총99억원, 주관기관: 국토교통연구인프라운영원)' 과제가 성공적으로 수행 완료되었다. 이 과제를 통해 총 53개의 표준실험절차서와 20개의 단체표준이 제정되었다. 이어서, 2단계 대형실험시설 준공완료에 따라 '건설분야 성능기반 표준시험절차 개발 사업(연구기간: '21.04~'25.12, 연구비: 총143.8억원)이 수행되고 있다. 현재, 국토교통연구인프라운영원은 이 사업 내 국제융합수리(주관기관: 한국농어촌공사 농어촌연구원), 극한성능(주관기관: 서울대학교) 및 기상환경재현(주관기관: 한국건설기술연구원) 표준실험절차 개발 과제에 공동기관으로 참여하여 단체표준 개발업무를 담당하고 있다. 본 논문에서는 단체표준 소개 및 현황에 대해 설명하고, 1단계 표준실험절차 개발과제를 통해 정립되었고 현재 2단계 표준실험절차 개발과제에도 적용되고 있는 시험관련 단체표준(안) 개발 프로세스에 대해 기술하고자 한다. 개발된 단체표준은 건설관련 실험·시험시설을 보유한 기관(대학, 공공기관, 산업체 연구소 등)의 성능검증 시험, 신기술 및 신제품 인증 표준절차에 활용가능하며, 시방서와 설계기준에 KS표준과 같이 시험 및 검사방법에 있어 참조표준으로도 활용 가능하다.

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Physicochemical Characteristics of Marinated Abalone Haliotis discus hannai in Seasoned Soy Sauce for the Elderly Using Texture Modification Technology (물성조절 기술을 활용한 고령친화식 전복(Haliotis discus hannai)장의 품질 특성)

  • Sohn Suk Kyung;Yeon Joo Bae;Sun Young Park;Hye Jeong Cho;Kil Bo Shim;Mi-soon Jang;Jaeyoung Oh;Jin-Soo Kim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.4
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    • pp.429-437
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    • 2024
  • This study aimed to develop and commercialize abalone Haliotis discus hannai marinated in seasoned soy sauce (AM-S) for the elderly by adjusting its texture (hardness) and strengthening its nutritional components. The number of pounding cycles [X1, 2-14 times] and heating time in a retort [X2, 5-19 min] were selected as independent variables, and hardness (Y1) and overall acceptance (Y2) were selected as dependent variables. The optimal conditions for X1 and X2 were 12 pounding cycles and heating in a retort for 17 min, respectively. The hardness values 209.2×103 N/m2 for the third method and 259.1×103 N/m2 for the first method. The nutritional value was 13.1 g/100 g crude protein and vitamins A, D, and C were not detected. The mineral content was 33.2 mg/100 g calcium and 258.7 mg/100 g potassium. Organisms from the coliform group and Escherichia coli were not detected. Therefore, AM-S for the elderly was classified as a hardness- and nutrient-controlled product based on the standards and specifications of senior-friendly foods provided in the MFDS and classified as the first step of senior-friendly foods according to the standards and specifications of senior-friendly foods provided in the KS.