• Title/Summary/Keyword: 16S-rRNA

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Comparison of Phylogenetic Characteristics of Bacterial Populations in a Quercus and Pine Humus Forest Soil (활엽수림과 침엽수림 부식토 내 세균군집의 계통학적 특성 비교)

  • Han, Song-Ih;Cho, Min-Hye;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.44 no.3
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    • pp.237-243
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    • 2008

  • Chemical and microbial characteristics of bacterial populations were investigated in a quercus and pine humus forest soil. Soil pH was $5.3\pm0.4$ and $4.1\pm0.9$ from each sample of a quercus and pine humus forest soil; C/N ratio of humus forest soil was $17.84\pm4.6%$ and $21.76\pm8%$, respectively. Total organic acid was investigated as 69.57 mM/g dry soil and 53.72 mM/g dry soil in each humus forest soil. Glutamine, pyruvate, succinate, lactic acid and acetic acid of pine humus forest soil were $1.5\sim4.5$ times higher than those of quercus humus forest soil. As we evaluated phylogenetic characteristics of bacterial populations by 16S rRNA-ARDRA analysis with DNA extracted from each humus forest soil. Based on the 16S rRNA sequences, 44 clone from ARDRA groups of quercus humus forest soil were classified into 7 phyla: ${\alpha},{\beta},{\gamma},{\delta}$-Proteobacteria, Acidobacteria, Actinobacteria, and Firmicutes. Thirty-two clone from ARDRA groups of pine humus forest soil were classified into 8 phyla: ${\alpha},{\beta},{\gamma}$-Proteobacteria, Acidobacteria, Bacteroides, Verrucomicrobia, Planctomycetes, and Gemmatomonadetes. According to PCA (Principal Component Analysis) based on 16S rRNA base sequence, there were three main groups of bacteria. All clone of Cluster I were originated from quercus humus forest soil, while 67% clone of Cluster II and 63% clone of Clusters III were separated from pine humus forest soil.

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Novel Sporichthyaceae Bacterium Strain K-07 Skin Barrier, Moisturizing and Anti-inflammatory Activity (신규 Sporichthyaceae Bacterium Strain K-07 배양액의 피부장벽, 보습 및 항염증 활성)

  • Lee, Dong-Geol;Kim, Minji;Kang, Seunghyun;Kim, Youn-Joon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.43 no.2
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    • pp.137-147
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    • 2017

  • The human skin is an ecosystem that provides habitat to various microorganisms. These comprise the skin microbiome and provide numerous benefits in addition to maintaining a symbiotic relation with the host. Various metabolites generated by the skin microbiome exert beneficial effects such as strengthening the skin barrier, and anti-aging and anti-inflammatory functions. In this study, we isolated a novel bacterium, designated Sporichthyacae strain K-07, from the human skin. Analysis of 16S rRNA gene sequences showed that the newly found bacterium shares 93.4% homology with the genus Sporichthya, thus corroborating the discovery of a novel genus. We further analyzed the effect of the novel strain in vitro, by treating HaCaT cells with bacterial metabolite products. Treatment resulted in changes in the mRNA expression levels of filaggrin, claudin1, claudin4, SMase, CERS3, HAS3, aquaporin3, IL-6, TNF-${\alpha}$, TSLP, and TARC. Specifically, the levels of filaggrin, claudin1, claudin4, SMase, CERS3, HAS3, and aquaporin3 were higher in strain K-07 metabolite product-treated cells than in control cells. These results showed that metabolite products of the novel strain K-07 enhanced the skin barrier and exert anti-inflammatory effects. Therefore, these metabolite products could be potentially used for treatment of skin conditions.

  • https://doi.org/10.15230/SCSK.2017.43.2.137 인용 PDF KSCI

Phylogenetic Relationship among the Bumblebees (Hymenoptera: Apidae) Common in Korea Inferred from Mitochondrial 16S rRNA Sequences

  • Yoon, Hyung-Joo;Lee, Myeong-Lyeol;Kim, Sam-Eun;Bae, Jin-Sik;Kim, Iksoo;Lee, Sang-Beom;Kim, Keun-Yong;Jin, Byung-Rae;Sohn, Hung-Dae
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.123-125
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    • 2003

  • We provide a molecular study intending to derive an estimate of the relationships within the genus Bombus, 10 species which span 6 subgenera common collected from several regions of Korea and B. terrestris imported from several foreign countries for pollination using a portion of mitochondrial 16S ribosomal RNA. (omitted)

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